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Influence of survivin and caspase-3 on cell apoptosis and prognosis in gastric carcinoma

World Journal of Gastroenterology 2004年第13期10卷 1984-1988页

作者： Yun-HongLi ChenWang Long-BangChert KuiMeng xiao-junzhou DepartmentofGastroenterology GulouHospitalMedicalCollegeofNanjingUniversityNanjing210008JiangsuProvinceChina MedicalCollegeofNanjingUniversity Nanjing210093JiangsuProvinceChina DepartmentofOncology ClinicalSchoolofMedicalCollegeofNanjingUniversity/NanjingGeneralHospitalofNanjingCommandNanjing210002JiangsuProvinceChina DepartmentofPathology ClinicalSchoolofMedicalCollegeofNanjingUniversity/NanjingGeneralHospitalofNanjingCommand.Nanjing210002JiangsuProvinceChina

AIM: To evaluate the role of survivin and caspase-3 in apoptosis of gastric carcinoma, as well as in prognosis of patients with gastric ***: Expressions of survivin and caspase-3 were investigated immunohistochemically in 80 gastric carcinoma patients without a history of chemo-radiation therapy. Tumor cell apoptosis was examined by TUNEL ***: Immunohistochemical analysis showed that survivin expression was positive in 61 of 80 patients (76%) with gastric carcinoma. In contrast, no expression of survivin in adjacent normal tissues was detected. Expression level of caspase-3 was higher in normal tissues than in *** with higher expression of survivin had worse histological grades and pathological stages. Expression of caspase-3 was significantly associated with histological stages, but not with the pathological stages. Although survivin expression in carcinoma was not inversely related to caspase-3, patients with survivin (-) and caspase-3(+) had the maximum apoptosis ***: Expression level of survivin was associated with histological grades and pathological stages of the tumor,indicating that survivin may be a poor prognosis factor for gastric carcinoma. Unlike caspase-3, survivin (an apoptosis inhibitor) can markedly inhibit the apoptosis of tumor cells.

关键词： caspase-3 细胞凋亡预后作用胃癌肿瘤免疫组织化学

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Significance of effector protease receptor-1 expression and its relationship with proliferation and apoptotic index in patients with primary advanced gastric adenocarcinoma

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World Journal of Gastroenterology 2004年第9期10卷 1262-1267页

作者： Xue-QuanYao Fu-KunLin Jie-ShouLi xiao-PingQi BoWu Hong-LinYin Heng-HuiMa Qun-LiShi xiao-junzhou SchoolofMedicine NanjingUniversityDepartmentofGeneralSurgeryJinlingHospital305ZhongshangdongRoadNanjing210002JingsuProvinceChina

AIM:To investigate the expression of effector protease receptor-1 (EPR-1),proliferative index Ki-67 and apoptosis index in patients with primary advanced gastric adenocarcinoma and to clarify the significance of EPR-1 expression and its correlationship with the proliferation and apoptosis ***:Using immmunohistochemical staining and terminal deoxynucleotidyl transferase mediated nick end labelling (TUNEL) technique,we determined the expression of EPR-1,proliferative index (Ki-67) and apoptotic index (AI) in 120 paraffin-embedded specimens of primary advanced gastric adenocarcinoma as well as lymph node metastasis and agjacent normal ***:EPR-1 expression was distributed in the cytoplasm of normal gastric mycoderma,carcinoma cells and smooth muscl *** plsitive rate of EPR-1 expression in the primary gastric adenocarcinomas,invasion tumor node and lymph node metastasis was 65.83%,55.29% and 68%,*** the positive rate in normal gastric mycoderma and smooth muscle cells was 46.7% and 53.3%,*** average positive rate of Ki-67 in EPR-1-positive tumors was 7.00% which was significantly lower than that of 8.53% in EPR-1-negative tumors,but the average AI in EPR-1-positive tumors was 1.25%,which was significantly higher than that of 1.00% observed in EPR-1-negative *** the other hand,the average positive labeling index for Ki-67(ki-67) in EPR-1-positive lymph node metastasis was 7.65%,which was significantly lower than that of 9.44% observed in EPR-1-negative lymph node ***,the average AI in EPR-1-positive lymph node metastasis tumors was 0.99%,which was significantly higher than that of .67% observed in EPR-1-negative lymph node ***:The frequency of EPR-1 exrpession was significantly higher in primary gastric adenocarcinoma and in its lymph node metastasis than that in normal gastric *** of EPR-1 Score is positively correlated with apoptosis index,but is negatively rea

关键词：原发性胃腺癌进展期效应器蛋白酶受体-1 细胞增殖细胞凋亡 TUNEL法

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DeepNoise: Signal and Noise Disentanglement Based on Classifying Fluorescent Microscopy Images via Deep Learning

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Genomics, Proteomics & Bioinformatics 2022年第5期20卷 989-1001页

作者： Sen Yang Tao Shen Yuqi Fang Xiyue Wang Jun Zhang Wei Yang junzhou Huang xiao Han Tencent AI Lab Shenzhen 518057China Department of Electronic Engineering The Chinese University of Hong KongHong Kong Special Administrative Region 999077China College of Computer Science Sichuan UniversityChengdu 610065China

The high-content image-based assay is commonly leveraged for identifying the phenotypic impact of genetic perturbations in biology ***,a persistent issue remains unsolved during experiments:the interferential technical noises caused by systematic errors(e.g.,temperature,reagent concentration,and well location)are always mixed up with the real biological signals,leading to misinterpretation of any conclusion ***,we reported a mean teacher-based deep learning model(Deep Noise)that can disentangle biological signals from the experimental ***,we aimed to classify the phenotypic impact of 1108 different genetic perturbations screened from 125,510 fluorescent microscopy images,which were totally unrecognizable by the human *** validated our model by participating in the Recursion Cellular Image Classification Challenge,and Deep Noise achieved an extremely high classification score(accuracy:99.596%),ranking the 2nd place among 866 participating *** promising result indicates the successful separation of biological and technical factors,which might help decrease the cost of treatment development and expedite the drug discovery *** source code of Deep Noise is available at https://***/Scu-sen/Recursion-Cellular-Image-Classification-Challenge.

关键词： Fluorescent microscopy image Biological signal Classification Deep learning Genetic perturbation

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MOLECULAR ENGINEERING STUDIES ON NONTHROMBOGENIC BIOMATERIALS——A NOVEL CLASS OF NONTHROMBOGENIC BIOMATERIALS WITH ZWITTERIONIC STRUCTURE OF CARBOXYBETAINES

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Chinese Journal of Polymer Science 2005年第1期23卷 93-102页

作者： junzhou Yong-zhiQiu xiao-pengZang Chang-wangPan QiangChen JianShen Si-congLin ResearchCenterofSurface＆InterfaceChemistryandEngineeringTechnology NanjingUniversityNanjing210093China

N,N-dimethyl-N-methacryloyloxyethyl-N-carboxyethyl ammonium(DMMCA)was graft-copolymerized onto thesurface of segmented poly(ether urethane)(SPEU)and PE *** carboxybetaine structure on SPEU and PE filmsurfaces was confirmed by ATR-FTIR,XPS and water contact angle *** the experiments with plateletadhesion and protein adhesion assay in vitro,the two materials studied,including poly-DMMCA gel,all show *** confirms once again that the zwitterionic molecular structure on the surfaces of materials isessential for improving their nonthrombogenicity and biocompatibility.

关键词： Nonthrombogenic biomaterials Zwitterionic biomaterial Carboxybetaine structure Surface graftcopolymerization.

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Components of Stem Barks of Winchia calophylla A. DC. and Their Bronchodilator Activities

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Journal of Integrative Plant Biology 2005年第7期47卷 892-896页

作者： Wei-MingZHU Hong-PingHE Yue-MaoSHEN junzhou xiao-JiangHAO StateKeyLaboratoryofPhytochemistryandPlantResourcesinWestChina KunmingInstituteofBotanytheChineseAcademyofSciencesKunming650204China KeyLaboratoryofMarineDrugs MinistryofEducationKeyLaboratoryofMarineDrugsofShandongProvinceMarineDrugandFoodInstituteOceanUniversityofChinaQingdao266003China

Abstract: The Dai medicinal plant Winchia calophylla A. DC. (Apocynaceae) has efficacy as an anti-cough and anti-asthmatic medication. In order to investigate its relative bioactive components, we studied the chemical constituents of this plant. Using repeated column chromatography, 28 compounds, including loganin, six phenolic compounds, 17 indole alkaloids, three pyridine alkaloids, and a quinoline alkaloid, were isolated from the stem barks of W. calophylla. Loganin, paeonol, N (4)-methyl akuammicine, and cantleyine exhibited a moderate relaxation effect on isolated smooth muscles of guinea-pig tracheal spirals and lung strips and may be the bioactive components responsible for the bronchodilation produced by W. calophylla.

关键词： alkaloid Apocynaceae bronchodilatation Winchia calophylla.

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Two New Monoterpene Diglycosides from Winchia calophylla ***

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Chinese Chemical Letters 2003年第10期14卷 1029-1032页

作者： WeiMingZHU BinGuiWANG WenYiKAN.G XmHONG junzhou xiaoJiangHAO DepartmentofChemistry YunnanNormalUniversityKunming650092 TheStateKeyLaboratoryofPhytochemistryandPlantResourcesinWestChina KunmingInstituteofBotanyChineseAcademyofSciences.Kunming650204

Two new monoterpene diglycosides, wincaloside A (1) and wincaloside B (2), along with loganin (3) were isolated from the stem barks of Winchia calophylla A. DC. The structures of 1 and 2 were established by spectrosco... 详细信息

关键词： Winchia calophylla Apocynaceae monoterpene diglycosides wincaloside.

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Pathological matrix stiffness promotes cardiac fibroblast differentiation through the POU2F1 signaling pathway

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Science China(Life Sciences) 2021年第2期64卷 242-254页

作者： Mingzhe Li Jimin Wu Guomin Hu Yao Song Jing Shen junzhou Xin Zijian Li Wei Liu Erdan Dong Ming Xu Youyi Zhang Han xiao Department of Cardiology and Institute of Vascular Medicine Peking University Third Hospital/NHC Key Laboratory of Cardiovascular Molecular Biology and Regulatory Peptides/Key Laboratory of Molecular Cardiovascular ScienceMinistry of Education/Beijing Key Laboratory of Cardiovascular Receptors ResearchBeijing 100191China Division of Cardiovascular Sciences Faculty of Biology Medicine and HealthUniversity of ManchesterManchester M139PTUK Institute of Cardiovascular Sciences Health Science CenterPeking UniversityBeijing 100191China

Cardiac fibroblast(CF)differentiation into myofibroblasts is a crucial cause of cardiac fibrosis,which increases in the extracellular matrix(ECM)*** increased stiffness further promotes CF differentiation and ***,the molecular mechanism is still *** used bioinformatics analysis to find new candidates that regulate the genes involved in stiffnessinduced CF differentiation,and found that there were binding sites for the POU-domain transcription factor,POU2F1(also known as Oct-1),in the promoters of 50 differentially expressed genes(DEGs)in CFs on the stiffer *** staining and Western blotting revealed that pathological stiffness upregulated POU2F1 expression and increased CF differentiation on polyacrylamide hydrogel substrates and in mouse myocardial infarction tissue.A chromatin immunoprecipitation assay showed that POU2F1 bound to the promoters of fibrosis repressors IL1R2,CD69,and *** expression of these fibrosis repressors was inhibited on pathological substrate *** of POU2F1 upregulated these repressors and attenuated CF differentiation on pathological substrate stiffness(35 kPa).Whereas,overexpression of POU2F1 downregulated these repressors and enhanced CF *** conclusion,pathological stiffness upregulates the transcription factor POU2F1 to promote CF differentiation by inhibiting fibrosis *** work elucidated the crosstalk between CF differentiation and the ECM and provided a potential target for cardiac fibrosis treatment.

关键词： fibroblast differentiation matrix stiffness POU2F1 cardiac fibrosis transcription factor

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Combined gene therapy of endostatin and interleukin 12 with polyvinylpyrrolidone induces a potent antitumor effect on hepatoma

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World Journal of Gastroenterology 2004年第15期10卷 2195-2200页

作者： Pei-YuanLi Ju-ShengLin Zuo-HuaFeng Yu-FeiHe He-junzhou XinMa xiao-KunCai De-AnTian InstituteofLiverDiseases TongjiHospitalTongjiWuhan430030HubeiProvinceChina DepartmentofBiochemistryandMolecularBiology TongjiMedicalCollegeHuazhongUniversityofScienceandTechnologyWuhan430030HubeiProvinceChina

AIM: To study the antitumor effect of combined gene therapy of endostatin and interleukin 12 (IL-12) with polyvinylpyrrolidone (PVP) on mouse transplanted ***: Mouse endostatin eukaryotic plasmid (pSecES) with a mouse Igk signal sequence inside and mouse IL-12 eukaryotic plasmid (pmIL-22) were transfected into BHK-22cells respectively. Endostatin and IL-22 were assayed by ELISA from the supernant and used to culture endothelial cells and spleen lymphocytes individually. Proliferation of the latter was evaluated by M-I-r. H22 cells were inoculated into the leg musde of mouse, which was injected intratumorallywith pSecES/PVP, pmIL-12/PVP or pSecES+pmIL-12/PVPrepeatedly. Tumor weight, serum endostatin and serumIL-22 were assayed. Tumor infiltrating lymphocytes, tumormicrovessel density and apoptosis of tumor cells were also displayed by HE staining, CD32 staining and ***: Endostatin and IL-12 were secreted after transfection, which could inhibit the proliferation of endothelial cells or promote the proliferation of spleen *** growth was highly inhibited by 92.8% after injection of pSecES+pmIL-i2/PVP accompanied by higher serum endostatin and IL-22, more infiltrating lymphocytes, fewertumor vessels and more apoptosis cells compared with injection of pSecES/PVP, pmIL-i2/PVP or vector/***: Mouse endostatin gene and IL-12 gene can be expressed after intratumoral injection with *** of hepatoma can be inhibited synergisticly,lymphocytes can be activated to infiltrate, and tumor cells are induced to apoptosis. Hepatoma can be highly inhibited or eradiated.

关键词：组合基因白细胞间介素12 聚乙烯吡咯烷酮 IL-12 感应抗癌作用肝细胞癌肿瘤 PVP

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氨基酸水溶肥料在黄瓜上的应用效果试验

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长江蔬菜 2023年第8期 68-70页

作者：宁俊周萧县农业技术推广中心安徽235200

为验证氨基酸水溶肥在黄瓜上的施用效果,萧县农技推广中心在黄瓜上开展了叶面喷施氨基酸水溶肥田间肥效试验。结果表明,氨基酸能促进黄瓜生长发育,提高果实品质,具有较好的增产和增收效应。叶面喷施氨基酸水溶肥比常规施肥增产297.9 kg/... 详细信息

为验证氨基酸水溶肥在黄瓜上的施用效果,萧县农技推广中心在黄瓜上开展了叶面喷施氨基酸水溶肥田间肥效试验。结果表明,氨基酸能促进黄瓜生长发育,提高果实品质,具有较好的增产和增收效应。叶面喷施氨基酸水溶肥比常规施肥增产297.9 kg/667 m^(2),净增产值582.6元/667 m^(2)。

关键词：黄瓜氨基酸水溶肥产量效益

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水溶性铜锌复合微肥对魔芋光合特性影响研究

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中国林副特产 2023年第4期 9-11页

作者：王正前王潇蒲泽文何紫梅刘春红黄军周广元市科学技术局四川广元628017 旺苍县水磨镇农业技术服务中心四川旺苍628200 广元市经济作物管理站四川广元628017 西南科技大学四川绵阳621000 广元市魔芋协会四川广元628017

水溶性铜锌复合微肥对花魔芋光合特性有很大影响:喷施2次后花魔芋叶片光合速率、蒸腾速率、叶绿素分别提高541.11%、64.20%、67.78%,胞间CO_(2)浓度降低35.88%;花魔芋地径、株高和叶盘分别增加23.3%、增加22.5%、25.5%;处理后的魔芋植... 详细信息

水溶性铜锌复合微肥对花魔芋光合特性有很大影响:喷施2次后花魔芋叶片光合速率、蒸腾速率、叶绿素分别提高541.11%、64.20%、67.78%,胞间CO_(2)浓度降低35.88%;花魔芋地径、株高和叶盘分别增加23.3%、增加22.5%、25.5%;处理后的魔芋植株更高,叶片更宽大,具有更大的光合面积和更强的光合特性,倒苗时间延后20天以上,总产量增加38.31%。

关键词：水溶性铜锌微肥魔芋光合特性

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