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快速酶解发酵法制备豆酱时酶解条件的优化

中国调味品 2017年第2期42卷 15-18,48页

作者：王晓婧汪建明李倩天津科技大学食品工程与生物技术学院天津300457

采用快速酶解发酵的方法制备豆酱,与传统发酵法相比,大大地缩短了生产周期。采用正交的方法对豆酱酶解过程进行优化,在以蛋白酶添加量、淀粉酶添加量、酶解时间、酶解温度、水料比为单因素试验的基础上,以氨基态氮含量和还原糖含量为指... 详细信息

采用快速酶解发酵的方法制备豆酱,与传统发酵法相比,大大地缩短了生产周期。采用正交的方法对豆酱酶解过程进行优化,在以蛋白酶添加量、淀粉酶添加量、酶解时间、酶解温度、水料比为单因素试验的基础上,以氨基态氮含量和还原糖含量为指标,进行了正交试验,得到的最佳工艺参数为:蛋白酶添加量2.5%,淀粉酶添加量4%,酶解时间7h,酶解温度60℃。验证实验表明:此豆酱在感官、口感方面与传统豆酱差异不显著,研究方法可行。

关键词：蛋白酶淀粉酶酶解氨基态氮还原糖

组织工程化皮肤:从形态和功能安全替代的前景

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中国组织工程研究 2017年第16期21卷 2600-2605页

作者：王晓静王国伟惠光艳赵铱民解放军海军青岛第一疗养院口腔科山东省青岛市266071 解放军海军第401医院崂山分院口腔科山东省青岛市266061 解放军第四军医大学口腔医院陕西省西安市710032

背景:组织工程学是近年来迅速发展起来的一门新学科,涉及皮肤,角膜、骨、软骨、肌腱等多个领域,组织工程化皮肤为近年年的研究热点,组织工程化皮肤为临床解决皮肤缺损的修复提供了良好的前景。目的:综述近年来组织工程化皮肤研究新进展... 详细信息

背景:组织工程学是近年来迅速发展起来的一门新学科,涉及皮肤,角膜、骨、软骨、肌腱等多个领域,组织工程化皮肤为近年年的研究热点,组织工程化皮肤为临床解决皮肤缺损的修复提供了良好的前景。目的:综述近年来组织工程化皮肤研究新进展。方法:由第一作者用计算机检索Medline数据库(1996年至2017年),检索词为"Epidermis;dermis;tissue engineered skin",语言设定英文,从表皮和真皮皮肤替代物对该领域的最新研究现状进行综述,探讨皮肤组织工程目前存在的问题及未来发展方向。结果与结论:纳入文献65篇。复合皮肤替代物虽然包括了表皮与真皮两层结构,但缺乏毛囊、汗腺和皮脂腺等皮肤附属器。如何解决组织工程化皮肤缺少附属器的问题,仍是目前皮肤组织工程研究的热点和重要方向。组织工程化皮肤的构建要在短时间内,生产出能够永久覆盖的皮肤替代物,并可将黑色素细胞和朗格罕氏细胞构建入组织工程皮肤替代物结构中去。现存的生物组织工程皮肤替代物既无法从形态上也无法从功能上完全替代皮肤组织,还需未来进行深入研究。

关键词：组织工程生物相容性材料皮肤组织构建皮肤黏膜组织构建表皮替代物真皮替代物黑色素细胞朗格罕氏细胞国家自然科学基金

TAMs对Smad4-/-头颈部鳞癌模型肿瘤干性的影响效应研究

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TAMs对Smad4-/-头颈部鳞癌模型肿瘤干性的影响效应研究

TAMs清除联合PI3K抑制剂对头颈鳞癌生长的影响及机制研究

2018年中华口腔医学会第十次全国口腔黏膜病学术大会暨第八次全国口腔中西医结合学术大会

作者：吴芳龙 xiao-jing wang 周红梅口腔疾病研究国家重点实验室 Department of Pathology University of Colorado

目的:肿瘤干细胞在肿瘤中扮演重要角色,研究发现肿瘤相关巨噬细胞(tumor associated macrophages, TAMs)可以调控肿瘤干性从而影响肿瘤进程,但在Smad4缺失型(Smad4-/-)头颈部鳞癌(headandnecksquamouscellcarcinomas,HNSCCs)中尚未见相关的研究报道。本研究将从肿瘤干性角度出发,探究TAMs在Smad4-/-HNSCCs动物模型中对肿瘤干性的影响及分子机制。方法:(1)采用腹腔注射氯膦酸盐脂质体清除小鼠体内的TAMs;(2)采用流式细胞术进行侧群(side population, SP)细胞和非SP细胞及其亚型的分选,并将分选出来的细胞进行二次成瘤;(3)通过WB、ELISA等检测方法探究影响肿瘤干性的可能分子机制。结果:(1)清除TAMs可抑制Smad4-/-HNSCCs的生长(P <0.05);(2)TAMs清除后下调SP细胞并上调非SP细胞(P <0.05);分选获得的SP细胞再次成瘤后发现,实验组SP细胞所形成的肿瘤生长速度慢于对照组(P <0.05);(3)肿瘤组织的IHC和血清ELISA检测均显示,TGF-β表达下降(P <0.05)。清除组中Sox2,TGF-β二型受体和pSmad3表达也下降。结论:在Smad4-/-HNSCCs的研究模型中,清除TAMs可能通过抑制非经典的TGF-β信号通路下调肿瘤干性,抑制肿瘤生长。

关键词： Smad4缺失头颈部鳞癌肿瘤相关巨噬细胞肿瘤干性肿瘤生长

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TAMs清除联合PI3K抑制剂对头颈鳞癌生长的影响及机制研究

Poorly controlled type II diabetes mellitus significantly enhances postoperative chemoresistance in patients with stage III colon cancer

2018年中华口腔医学会第十次全国口腔黏膜病学术大会暨第八次全国口腔中西医结合学术大会

作者：吴芳龙 xiao-jing wang 周红梅口腔疾病研究国家重点实验室四川大学华西口腔医院口腔黏膜病科 Department of Pathology University of Colorado

目的:在Smad4敲除引起自发性头颈部鳞状细胞癌(headandneck squamous cell carcinomas, HNSCCs)的小鼠模型(Smad4-/-HNSCCs)中,课题组发现有大量的肿瘤相关巨噬细胞(tumorassociatedmacrophages, TAMs)浸润,提示TAMs可能发挥促进作用。本研究将以TAMs为研究对象,探究TAMs在Smad4-/-HNSCCs动物模型中的作用及其分子机制。方法:(1)采用腹腔注射氯膦酸盐脂质体清除小鼠体内的TAMs;(2)观察TAMs清除后对荷瘤小鼠肿瘤的影响,用TUNEL和WB检测细胞凋亡及可能的机制;(3)通过WB等检测手段探究TAMs清除后对细胞增殖的影响及可能的机制,并联合PI3K信号通路抑制剂,观察对肿瘤的影响。结果:(1)清除TAMs可以显著抑制Smad4-/-HNSCCs的生长(P <0.05);(2)清除TAMs可上调Caspase信号通路促进细胞的凋亡;(3)TAMs清除后激活PI3K信号通路可能使肿瘤维持较高的增殖,而联合应用PI3K信号通路抑制剂,可显著抑制肿瘤的增殖(P <0.05),使肿瘤维持惰性的生长模式。结论:在Smad4-/-HNSCCs研究模型中,清除TAMs可诱导凋亡从而抑制肿瘤的生长;同时可以激活PI3K信号通路,而联合应用PI3K信号通路抑制剂具有更好的抑癌效果。

关键词： Smad4缺失头颈部鳞癌肿瘤相关巨噬细胞肿瘤生长 PI3K抑制剂

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World Journal of Gastroenterology 2025年第3期31卷 29-41页

作者： Ruo-Yu Guan Jia-Wei Wu Zi-Yun Yuan Zhi-Yuan Liu Zi-Zhu Liu Zhi-Cong xiao jing-Hui Li Cheng-Zhi Huang Jun-Jiang wang Xue-Qing Yao Guangdong Cardiovascular Institute Guangdong Provincial People’s HospitalGuangdong Academy of Medical SciencesSouthern Medical UniversityGuangzhou 510080Guangdong ProvinceChina Department of Gastrointestinal Surgery Department of General SurgeryGuangdong Provincial People’s Hospital(Guangdong Academy of Medical Sciences)Southern Medical UniversityGuangzhou 510080Guangdong ProvinceChina Department of General Surgery Guangdong Provincial People’s Hospital Ganzhou Hospital(Ganzhou Municipal Hospital)Ganzhou 341000Jiangxi ProvinceChina Shantou University Medical College Shantou 515041Guangdong ProvinceChina School of Medicine South China University of TechnologyGuangzhou 510006Guangdong ProvinceChina Gannan Medical University Ganzhou 341000Jiangxi ProvinceChina

BACKGROUND Type II diabetes mellitus(T2DM)has been associated with increased risk of colon cancer(CC)and worse prognosis in patients with *** effects of T2DM on postoperative chemoresistance rate(CRR)and long-term disease-free survival(DFS)and overall survival(OS)in patients with stage III CC who receive curative resection remain *** To investigate whether T2DM or glycemic control is associated with worse postoperative survival outcomes in stage III *** This retrospective cohort study included 278 patients aged 40-75 years who underwent surgery for stage III CC from 2018 to *** on preoperative T2DM history,the patients were categorized into non-DM(n=160)and DM groups(n=118).The latter was further divided into well-controlled(n=73)and poorly controlled(n=45)groups depending on the status of glycemic ***,OS,and CRR were compared between the groups and Cox regression analysis was used to identify risk *** Patients in the DM and non-DM groups demonstrated similar DFS,OS,and CRR(DFS:72.03%vs 78.75%,P=0.178;OS:81.36%vs 83.12%,P=0.638;CRR:14.41%vs 7.5%,P=0.063).Poorly controlled DM was associated with a significantly worse prognosis and higher CRR than well-controlled DM(DFS:62.22%vs 78.07%,P=0.021;OS:71.11%vs 87.67%,P=0.011;CRR:24.40%vs 8.22%,P=0.015).High preoperative fasting plasma glucose[DFS:Hazard ratio(HR)=2.684,P<0.001;OS:HR=2.105,P=0.019;CRR:HR=2.214,P=0.005]and glycosylated hemoglobin levels(DFS:HR=2.344,P=0.006;OS:HR=2.119,P=0.021;CRR:HR=2.449,P=0.009)indicated significantly poor prognosis and high CRR,while T2DM history did not(DFS:HR=1.178,P=0.327;OS:HR=0.933,P=0.739;CRR:HR=0.997,P=0.581).CONCLUSION Increased preoperative fasting plasma glucose and glycosylated hemoglobin levels,but not T2DM history,were identified as risk factors associated with poor postoperative outcomes and high CRR in patients with stage III CC.

关键词： Colon cancer Chemoresistance Diabetes mellitus Prognosis Type II diabetes mellitus

BIBR1532 inhibits proliferation and metastasis of esophageal squamous cancer cells by inducing telomere dysregulation

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World Journal of Gastrointestinal Oncology 2025年第1期17卷 214-225页

作者： Qin wang Qing-Rong Li Lei Xu Zi-Chun Yuan xiao Liu Mao-Ju Tang Man Luo xiao-Wu Zhong Qiang Ma xiao-Lan Guo Department of Clinical Laboratory Affiliated Hospital of North Sichuan Medical CollegeNanchong 637000Sichuan ProvinceChina School of Laboratory Medicine&Translational Medicine Research Center North Sichuan Medical CollegeNanchong 637000Sichuan ProvinceChina

BACKGROUND Esophageal squamous cell carcinoma(ESCC)is a malignant tumor with high morbidity and mortality,and easy to develop resistance to chemotherapeutic *** are DNA-protein complexes located at the termini of chro-mosomes in eukaryotic cells,which are unreplaceable in maintaining the stability and integrity of ***,an RNA-dependent DNA polymerase,play vital role in telomere length maintain,targeting telomerase is a promising therapeutic strategy for ***150 and KYSE410 cells were cultured and exposed to various concentrations of *** viability was assessed at 48 hours and 72 hours to determine the IC50 *** effects of BIBR1532 on ESCC cell proliferation,migration,and cellular senescence were evaluated using the cell counting kit-8 assay,plate colony formation assay,scratch assay,transwell assay,andβ-galactosidase staining,*** blotting was performed to detect the expression of RESULTS The IC50 values for KYSE150 and KYSE410 cells after 48 hours of BIBR1532 exposure were 48.53μM and 39.59μM,*** values decreased to 37.22μM and 22.71μM,respectively,following a longer exposure of 72 ***1532 exhibited dose-dependent effects on KYSE150 and KYSE410 cells,including decreased hTERT expression,inhibition of proliferation and metastasis,and induction of cellular ***,BIBR1532 upregulated the expression of the DDR protein,γ-H2AX,and activated the ataxia telangiectasia and Rad3-related protein(ATR)/check point kinase 1(CHK-1)and ataxia-telangiectasia mutated gene(ATM)/CHK2 ***1532 downregulated the expression of telomere-binding proteins,including telomeric-repeat binding factor 1(TRF1),TRF2,protection of telomeres 1,and TIN2-interacting protein *** a nude mouse xenograft model,BIBR1532 significantly suppressed tumor growth,reduced hTERT expression,and increasedγ-H2AX protein *** and eosin staining of various organs,including the heart,liver,s

关键词： Esophageal squamous cell carcinoma BIBR1532 Human telomerase reverse transcriptase DNA damage response Telomere-binding proteins

Unraveling brain aging through the lens of oral microbiota

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Neural Regeneration Research 2025年第7期20卷 1930-1943页

作者： Qinchao Hu Si wang Weiqi Zhang jing Qu Guang-Hui Liu State Key Laboratory of Membrane Biology Institute of ZoologyChinese Academy of SciencesBeijingChina Hospital of Stomatology Guanghua School of StomatologyGuangdong Provincial Key Laboratory of StomatologySun Yat-sen UniversityGuangzhouGuangdong ProvinceChina Advanced Innovation Center for Human Brain Protection and National Clinical Research Center for Geriatric Disorders Xuanwu HospitalCapital Medical UniversityBeijingChina Aging Translational Medicine Center International Center for Aging and CancerBeijing Municipal Geriatric Medical Research CenterXuanwu HospitalCapital Medical UniversityBeijingChina CAS Key Laboratory of Genomic and Precision Medicine Beijing Institute of GenomicsChinese Academy of Sciences and China National Center for BioinformationBeijingChina University of Chinese Academy of Sciences BeijingChina Institute for Stem Cell and Regeneration Chinese Academy of SciencesBeijingChina Aging Biomarker Consortium BeijingChina State Key Laboratory of Stem Cell and Reproductive Biology Institute of ZoologyChinese Academy of SciencesBeijingChina Beijing Institute for Stem Cell and Regenerative Medicine BeijingChina

The oral cavity is a complex physiological community encompassing a wide range of *** of oral microbiota can lead to various oral infectious diseases,such as periodontitis and tooth decay,and even affect systemic health,including brain aging and neurodegenerative *** studies have highlighted how oral microbes might be involved in brain aging and neurodegeneration,indicating potential avenues for intervention *** this review,we summarize clinical evidence demonstrating a link between oral microbes/oral infectious diseases and brain aging/neurodegenerative diseases,and dissect potential mechanisms by which oral microbes contribute to brain aging and *** also highlight advances in therapeutic development grounded in the realm of oral microbes,with the goal of advancing brain health and promoting healthy aging.

关键词： Alzheimer's disease brain aging multiple sclerosis neurodegeneration neurodegenerative diseases oral microbiota Parkinson's disease periodontitis bacteria Porphyromonas gingivalis

Effects of Shenqi Xiangyi granules in advanced gastric cancer chemotherapy

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World Journal of Gastrointestinal Oncology 2025年第1期17卷 169-175页

作者： xiao-jing Shi Yu Song Xue-Xue Liang Ting Chen Huang-Yu Hao Xue Han Ya-Nan Chen Department of Oncology Zhangjiagang First People's HospitalSuzhou 215600Jiangsu ProvinceChina

BACKGROUND Owing to the absence of specific symptoms in early-stage gastric cancer,most patients are diagnosed at intermediate or advanced *** a result,treatment often shifts from surgery to other therapies,with chemotherapy and targeted therapies being the primary options for advanced gastric cancer treatment.A total of 116 patients with advanced gastric cancer,admitted from January 2021 to December 2023,were selected and divided into two groups of 58 each using the random number table *** control group received FOLFOX4 chemothe-rapy(oxaliplatin+calcium+folinate+5-fluorouracil)combined with intravenous *** observation group received the same treatment as the control group,supplemented by oral administration of Senqi Shiyiwei *** groups underwent treatment cycles of 3 weeks,with a minimum of two *** therapeutic efficacy,immune mechanisms,and treatment-related toxicity and side effects were compared between the *** objective remission rate in the observation group(55.17%)was higher than that of the control group(36.21%)(P0.05).CONCLUSION Adjuvant therapy with Shenqixian granules may enhance the efficacy of simudizumab combined with FOLFOX4 chemotherapy in advanced gastric cancer and the immune function by increasing immune cell counts,making it a valuable option in clinical treatment.

关键词： Sindilizumab FOLFOX4 chemotherapy Advanced gastric cancer Ginseng Qi Xiangyi granule Clinic treatment

南京市产业结构与用水结构互动耦合机制及关联分析

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湖南师范大学自然科学学报 2017年第4期40卷 8-13页

作者：陈晓李景保王飞徐志湖南师范大学资源与环境科学学院中国长沙410081

基于2005—2014年南京市产业结构和用水结构的相关统计数据,运用灰色关联分析方法,构建产业结构与用水结构的关联度和耦合度模型,研究南京市产业结构与用水结构的互动耦合机制及其关联性,结果表明:南京市产业结构与用水结构关联度在0.5... 详细信息

基于2005—2014年南京市产业结构和用水结构的相关统计数据,运用灰色关联分析方法,构建产业结构与用水结构的关联度和耦合度模型,研究南京市产业结构与用水结构的互动耦合机制及其关联性,结果表明:南京市产业结构与用水结构关联度在0.5以上,属于中等关联;产业结构对用水结构的关联序为第三产业(0.566)、第一产业(0.563)、第二产业(0.557),用水结构对产业结构的关联序为生活用水(0.696)、农业用水(0.601)、工业用水(0.582);南京市产业结构与用水结构耦合的空间差异明显,主城区属于协调型,江宁区与六合区属于磨合型,浦口区、高淳区与溧水区属于拮抗型;南京市产业结构与用水结构的耦合度在时间上显示出阶段性波动的特点,第一阶段(2005—2007年)属于磨合时期,第二阶段(2008—2010年)从磨合转向拮抗时期,第三阶段(2011~2014年)逐渐向协调方向发展.

关键词：产业结构用水结构灰色关联分析耦合度南京市