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Insect Science 2022年第2期29卷 333-343页

作者： xiao-jian liu Guo Jun xiao-Yu Liang Xue-Yao Zhang Ting-Ting Zhang Wei-Min liu jian-Zhen Zhang Min Zhang Research Institute of Applied Biology Shanxi UniversityTaiyuanChina

Ecdysone-induced protein 93F(E93)plays important roles during the metamorphosis process in *** this study,a cDNA of the LmE93 gene was identified from the transcriptome of Locusta migratoria,which consists of the 3378-nucleotide open-reading frame(ORF)and encodes 1125 amino acids with helix–turn–helix(HTH)*** transcription quantitative polymerase chain reaction analysis revealed that LmE93 was highest expressed in *** LmE93 expression level was markedly low from the 3rd to 4th instar nymphs,and greatly increased in 1-day-old 5th instar nymphs with a peak on middle nymphal days,then declined in the late nymphal ***,injected dsLmE93 into 4th and 5th instar nymphs greatly reduced LmE93 transcripts,respectively,and prevented the process of metamorphosis,causing supernumerary nymphal ***–eosin staining of the integument showed that the apolysis occurred in advance in 4th instar nymphs,and old cuticle degradation was decreased in dsLmE93-injected locusts of 5th instar *** and no fully developed wings with reduced columns between the anterior and posterior regions were found in N6 and N7 supernumerary *** addition,the development of the ovary in dsLmE93-injected locusts was severely blocked,the yolk was almost not formed and there was no development of *** results indicated that LmE93 play key roles in the metamorphosis,cuticle,wing and ovarian development of locusts.

关键词： expression function Locusta migratoria RNA interference transcription factor E93

Material selection combined with optimal structural design for mechanical parts

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Journal of Zhejiang University-Science A(Applied Physics & Engineering) 2013年第6期14卷 383-392页

作者： Le-miao QIU Liang-feng SUN xiao-jian liu Shu-you ZHANG State Key Laboratory of Fluid Power Transmission and Control Zhejiang University

To reduce the environmental impact of mechanical parts, an approach integrating structural design and material selection was studied. Adding the discrete variable of material, a hybrid optimization model was built with the aim of minimizing environmental impact and based on an ordinary structure optimization model. An optional material set was built by combining measures of qualitative and quantitative screening, while the lifecycle environmental impact of the materials was quantified using the method of Eco-indicator 99. Two groups of structurally optimal solutions were calculated with ideal and negative-ideal materials selected respectively, and then the hybrid model was simplified by comparing the solutions. A material environmental performance index was calculated using an analytic method. By comparing this index for every material in the optional material set, the optimal material can be found and the structural solutions calculated. This method was applied to a dowel bar design process as a case study. The results show that the environmental impact of each material has a significant effect on the optimal structural solution, and it is necessary to study the integration of structural design and material selection.

关键词： Green design Structure optimization Material selection Environmental performance index

Identification of LmUAP1 as a 20-hydroxyecdysone response gene in the chitin biosynthesis pathway from the migratory locust, Locusta migratoria

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Insect Science 2018年第2期25卷 211-221页

作者： xiao-jian liu Ya-Wen Sun Da-Qi Li Sheng Li En-Bo Ma[] jian-Zhen Zhang Research Institute of Applied Biology College of Life Science Shanxi University Taiyuan China Key Laboratory of Insect Developmental and Evolutionary Biology Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai China

In Locusta migratoria, we found that two chitin biosynthesis genes, UDP N-acetylglucosamine pyrophosphorylase gene LmUAP1 and chitin synthase gene Lm- CHS1, are expressed mainly in the integument and are responsible for cuticle formation. However, whether these genes are regulated by 20-hydroxyecdysone （20E） is still largely unclear. Here, we showed the developmental expression pattern ofLmUAP1, LmCHS1 and the corresponding 20E titer during the last instar nymph stage of locust. RNA interference （RNAi） directed toward a common region of the two isoforms of LmEcR （LmEcRcom） reduced the expression level of LmUAP1, while there was no difference in the expression of LmCHS1. Meantime, injection of 20E in vivo induced the expression of LmUAP1 but not LmCHS1. Further, we found injection-based RNAi of LmEcRcom resulted in 100% mortality. The locusts failed to molt with no apolysis, and maintained in the nymph stage until death. In conclusion, our preliminary results indicated that LmUAP1 in the chitin biosynthesis pathway is a 20E late-response gene and LmEcR plays an essential role in locust growth and development, which could be a good potential target for RNAi-based pest control.

关键词： chitin synthesis Locusta migratoria regulation RNA interference 20-hydroxyecdysone

A knowledge push technology based on applicable probability matching and multidimensional context driving

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Frontiers of Information Technology & Electronic Engineering 2018年第2期19卷 235-245页

作者： Shu-you ZHANG Ye GU xiao-jian liu jian-rong TAN State Key Laboratory of Fluid Power & Mechatronic Systems Zhejiang University

Actively pushing design knowledge to designers in the design process, what we call ‘knowledge push＇, can help improve the efficiency and quality of intelligent product design. A knowledge push technology usually includes matching of related knowledge and proper pushing of matching results. Existing approaches on knowledge matching commonly have a lack of intelligence. Also, the pushing of matching results is less personalized. In this paper, we propose a knowledge push technology based on applicable probability matching and multidimensional context driving. By building a training sample set, including knowledge description vectors, case feature vectors, and the mapping Boolean matrix, two probability values, application and non-application, were calculated via a Bayesian theorem to describe the matching degree between knowledge and content. The push results were defined by the comparison between two probability values. The hierarchical design content models were built to filter the knowledge in push results. The rules of personalized knowledge push were sorted by multidimensional contexts, which include design knowledge, design context, design content, and the designer. A knowledge push system based on intellectualized design of CNC machine tools was used to confirm the feasibility of the proposed technology in engineering applications.

关键词： Product design Knowledge push Applicable probability matching Multidimensional context Personalization

Roles of LmCDA1 and LmCDA2 in cuticle formation in the foregut and hindgut of Locusta migratoria

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Insect Science 2021年第5期28卷 1314-1325页

作者： Min Zhang Peng-Juan Ma Ting-Ting Zhang Zhi-Mei Gao Pan Zhao xiao-Jjian liu Xue-Yao Zhang Wei-Min liu Rong-Rong Yu Bernard Moussian jian-Zhen Zhang Research Institute of Applied Biology Shanxi UniversityTaiyuanChina School of Life Science Shanxi UniversityTaiyuanChina Department of Biology Taiyuan Normal UniversityTaiyuanChina iBV Universite Cote d'AzurParc ValroseFrance

Chitin deacetylases(CDAs,including CDAI and CDA2)are considered key enzymes for body cuticle formation and tracheal morphogenesis in various insect ***,their functions in the formation of the cuticular intima of the foregut and hindgut are ***,we investigated the roles of their respective genes LmCDAI and Lm-CD42 in this process,in the hemimetabolous insect Locusta *** of LmCDAI and LmCDA2 were highly expressed both before and after molting in the *** the hindgut,their expression was high only before *** both the foregut and hindgut,LmCDA1 protein was localized in the basal half of the chitin matrix(procuti-cle),whereas LmCDA2 was detected in the upper half of the procuticle.K nockdown of LmCDAI by RNA interference(RNAi)in 5th-instar nymphs caused no visible defcts of the hindgut *** contrast,the chitinous lamellae of the cuticular intima in the foregut of knockdown animals were less compact than in control *** against LmCDA2 led to thickening of both the foregut and hindgut cuticles,with a greater number of thinner laminae than in the respective control *** together,our results show that LmCDAI and LmCDA2 have distinct,but overlapping,functions in chitin organization in the foregut ***,in the hindgut,this process seems independent of LmCDA1 activity but requires LmCDA2 ***,the CDAs reflect tissue-specific differences in cuticular organization and function,which need further detailed molecular and histological analyses for full comprehension.

关键词： CDA:cuticle digestion foregut hindgut laminae

MicroRNA-298 determines the radio-resistance of colorectal cancer cells by directly targeting human dual-specificity tyrosine(Y)-regulated kinase 1A

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World Journal of Gastrointestinal Oncology 2024年第4期16卷 1453-1464页

作者： Mei-Zhu Shen Yong Zhang Fang Wu Mei-Zhen Shen Jun-Lin Liang xiao-Long Zhang xiao-jian liu Xin-Shu Li Ren-Sheng Wang Department of Radiotherapy The First Affiliated Hospital of Guangxi Medical UniversityNanning 530021Guangxi Zhuang Autonomous RegionChina Department of Radiotherapy People’s Hospital of Guangxi Zhuang Autonomous RegionNanning 530021Guangxi Zhuang Autonomous RegionChina Department of Colorectal Anal Surgery The First Affiliated Hospital of Guangxi Medical UniversityNanning 530021Guangxi Zhuang Autonomous RegionChina Department of Clinical Medicine Guangxi Medical UniversityNanning 530021Guangxi Zhuang Autonomous RegionChina

BACKGROUND Radiotherapy stands as a promising therapeutic modality for colorectal cancer(CRC);yet,the formidable challenge posed by radio-resistance significantly undermines its efficacy in achieving CRC *** To elucidate the role played by microRNA-298(miR-298)in CRC *** To establish a radio-resistant CRC cell line,HT-29 cells underwent exposure to 5 gray ionizing radiation that was followed by a 7-d recovery *** quantification of miR-298 levels within CRC cells was conducted through quantitative RT-PCR,and protein expression determination was realized through Western *** viability was assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and proliferation by clonogenic ***-induced apoptosis was discerned through flow cytometry *** We observed a marked upregulation of miR-298 in radio-resistant CRC ***-298 emerged as a key determinant of cell survival following radiation exposure,as its overexpression led to a notable reduction in radiation-induced ***,miR-298 expression exhibited a strong correlation with CRC cell *** investigation unveiled human dual-specificity tyrosine(Y)-regulated kinase 1A(DYRK1A)as miR-298’s direct *** Taken together,our findings underline the role played by miR-298 in bolstering radio-resistance in CRC cells by means of DYRK1A downregulation,thereby positioning miR-298 as a promising candidate for mitigating radioresistance in CRC.

关键词： MicroRNA-298 Human dual-specificity tyrosine(Y)-regulated kinase 1A Colorectal cancer Radio-resistance p53 binding protein 1

Interactive Decision Support Algorithm and Its Application

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Journal of Systems Science and Systems Engineering 2001年第4期13卷 475-480页

作者： HONG xiao-kang, liu jian-lin, XIE jian-cang, liu Fu-chao, MA Bin Xi’an University of Technology, Xi’an 710048, China Xi'an University of Technology Xi'an 710048 China

On the bases of the properties of abstract hierarchical structure model and the concrete structure of the mode1 system. which is convenient to solve practical problems, a visual interactive hierarchical coordination method has been proposed. In this paper, a compensation adjustment sub-mode1 for hydropower stations, an optimal operation sub-model for hydro-thermal power systems, and an aggregation model based on the aspiration level theory are built, and these models can be solved with decision support algorithm. The set of objectives and its structure could be made by the decision-maker in visua1 software, which could be decided by AHP. Finally, the application results show that this methodology is feasible, however, the software (DSS) needs further improvement.

关键词： interactive decision support a1gorithm mu1ti-objective and multi-mode1 structurel hydro- thermal power system scheme of power production

Phosphatase and tensin homolog gene inhibits the effect induced by gonadotropin-releasing hormone subtypes in human endometrial carcinoma cells

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Chinese Medical Journal 2010年第9期123卷 1170-1175页

作者： ZHAO Li-jun liu Ning LI xiao-ping WANG jian-liu WEI Li-hui Department of Obstetrics & Gynecology Peking University People's Hospital100044BeijingChina

Background Type I gonadotropin-releasing hormone （GnRH-l） agonists have been applied for the treatment of steroid-dependent tumors such as breast carcinoma, ovarian cancer and prostatic carcinoma. But the mechanism has not been clarified yet. There are few reports about the treatment of endometrial carcinoma using GnRH-l agonists. Type II GnRH （GnRH-ll） is a new subtype of GnRH. Our aim was to investigate the effects of GnRH-l agonists and GnRH-ll on estrogen receptor-negative human endometrial carcinoma cells and the effect from phosphatase and tensin homolog gene （PTEN） to *** A lentiviral vector-mediated RNAi method was used to establish a PTEN-negative HEC-1A cell clone （HEC-1A-ND）. MTT and flow cytometry were used to detect the cell proliferation, cell cycle and apoptosis of HEC-1A, HEC-1A-NC and HEC-1A-ND cells after treatment with GnRH-l agonist Triptorelin （10-11 mol/L to 10-5 mol/L） or GnRH-ll （10-11 mol/L to 10-5 mol/L）. Western blotting was used to detect AKT and ERK1/2 activation after treatment with different concentrations of Triptorelin or GnRH-ll for 30 minutes in the above mentioned three kinds of cells. Results Triptorelin and GnRH-ll induced apoptosis and inhibited proliferation of HEC-1 A, HEC-1A-ND and HEC-1A-NC in a dose-dependent manner. This effect was augmented in HEC-1 A-ND cells in which PTEN gene was knocked-down. Furthermore, Triptorelin and GnRH-ll inhibited the AKT and ERK activity in HEC-1 A-ND *** Triptorelin and GnRH-ll can promote apoptosis rate and inhibit cell proliferation of estrogen receptor-negative endometrial carcinoma cells in a dose-dependent manner. PTEN gene can inhibit the effects of Triptorelin or GnRH-ll on human endometrial carcinoma cells.

关键词： human endometrial carcinoma gonadotropin-releasing hormone-I agonist estrogen receptor

Improvement of Production Performance of Wool by Coat Treatment in Fine-Wool Sheep

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Animal Husbandry and Feed Science 2011年第6期3卷 42-44,48页

作者： SUN xiao-ping liu jian-bin LANG Xia GUO jian Lanzhou Institute of Animal & Veterinary Pharmaceutics Science of Chinese Academy of Agricultural Sciences and Gansu Key Laboratory of YakBreeding Engineering Lanzhou 730050 China

[ Objective] To explore the effects of coats on production and quality of wool fibers in fine-wool sheep. [ Method] The coats made of pelypropylene were put on fine-wool sheep after shearing and taken off next year, The effects of coats on structure, production and quality of wool f bers, wool sweat secretion, percentage of clean wools, impurities and wool textile process in fine-wool sheep were observed, [ Result] After the sheep were dressed one year later, the average fiber length was increased by 0.33 cm （P 〈 0.05） ; the percentage of clean wools was increased by 12.44% （ P 〈 0.01 ） ; the yield of clean wools were increased by 0.44 kg （ P 〈 0.01 ） ; the wool grease content was increased by 6.44% （ P 〈 0.01 ） ; the length of polluted staple was decreased by 3.10 cm （ P 〈 0.01 ） ; and the impurity content was decreased by 21.25% （ P 〈 0.01 ）. [ Conclusion] Coats remarkably improve quality of wool fibers and production performance in fine-wool sheep.

关键词： Coat Improvement Wool Production Quality

Development of a micro-monitoring system based on the liquid crystal display

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Chinese Journal of Biomedical Engineering(English Edition) 2002年第3期11卷 133-139页

作者： DU xiao-lan,WU Bao-ming,HE Qing-hua, xiao jian, liu Yan

objective: To introduce the development of a portable multi-parameter monitoring system. Methods: Using signal detecting and data processing techniques, this monitor with a graphic liquid crystal display (LCD) and a single-chip microprocessor 8Xc196Kc can dynamically measure electrocardiogram(ECG), breath wave, blood pressure (BP), breath frequency (BF), heart rate (HR) and body temperature (BT) in real-time. Results: This monitor can dynamically display the ECG, breath wave and other parameters in real time. It can also provide a 24h trend graph for each measured parameter and exchange data, through the serial communication interface (RS232), with the PC or hand-held PC (HPC). Conclusions: This portable monitoring system has a man-machine interface friendly. It is capable of performing continuous monitoring and also used as terminal of a central monitoring system or a tele-monitoring system.

关键词： monitor single-chip microcomputer LCD