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Tescalcin is an unfavorable prognosis factor that regulats cell proliferation and survival in hepatocellular carcinoma patients

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Cancer Communications 2020年第8期40卷 355-369页

作者： Zhong-Guo Zhou Jin-Bin Chen Rong-Xin Zhang Ling Ye Jun-ChengWang Yang-Xun Pan xiao-huiwang Wen-Xuan Li Yao-Jun Zhang Li Xu Min-Shan Chen State Key Laboratory of Oncology in South China Collaborative Innovation Center for Cancer MedicineSun Yat-sen University Cancer CenterGuangzhouGuangdong 510060P.R.China Department of Liver Surgery Sun Yat-sen University Cancer CenterGuangzhouGuangdong 510060P.R.China Department of Colorectal Cancer Sun Yat-sen University Cancer CenterGuangzhouGuangdong 510060P.R.China Department of Oncology The First Affiliated Hospital of Jinan UniversityGuangzhouGuangdong 510630P.R.China

Background:Hepatocellular carcinoma(HCC)is a major health problem and a primary cause of cancer-related death *** great advances have achieved recently by large-scale high-throughput analysis,the precise molecular mechanism underlying HCC progression remains to be clearly *** investigated the relationship between Tescalcin(TESC),a candidate oncogene,and clinicopathological features of HCC patients and explored the role of TECS in HCC ***:To identify new genes involved in HCC development,we analyzed The Cancer Genome Atlas liver cancer database,and TESC was selected for further *** tissue microarray analysis for TESC and its association with clinicopathological features were performed to investigate its clinical *** was knocked down by using short-hairpin *** proliferation was analyzed by WST-1 assay and cell *** apoptosis was tested by fluorescence-activated cell sorting.A subcutaneous xenograft tumor model in nude mice was established to determine the in vivo function of *** microarray was used to identify its molecular ***:TESC was significantly increased in HCC tissues compared with the adjacent normal liver *** expression of TESC was detected in 61 of 172 HCC patients by tissue *** tumor(>5 cm)and elevated total bilirubin were associated with high TESC expression(both P<0.050).In multivariate analysis,TESC was identified as an independent prognostic factor for short overall survival of HCC *** knockdown impaired HCC cell growth in vitro and in *** knockdown significantly increased cell apoptosis in HCC cell ***,Affymetrix microarray analysis revealed that TESC knockdown inhibited tumor proliferation-related pathways while activated cell death-related ***:TESC was identified as an independent prognostic factor for short overall survival of HCC patients,and was critical for HCC cell proli

关键词： Apoptosis Cell proliferation Hepatocellular carcinoma Oncogene Tescalcin

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短程硝化/厌氧氨氧化工艺处理氧化铁红废水

中国给水排水 2019年第1期35卷 102-106页

作者：冯兴会汪晓军陈振国陈婧华南理工大学环境与能源学院工业聚集区污染控制与生态修复教育部重点实验室广东广州510006 佛山市化尔铵生物科技有限公司广东佛山528300

采用短程硝化/厌氧氨氧化工艺对氧化铁红废水进行脱氮处理,分析沸石曝气生物滤池(Z-BAF)亚硝化的可行性,以及碱度和氨氮的比值对亚硝化作用的影响,并对比了Na_2CO_3和NaHCO_3作为碱度供体的经济成本。结果表明,废水经Z-BAF生化处理后,... 详细信息

采用短程硝化/厌氧氨氧化工艺对氧化铁红废水进行脱氮处理,分析沸石曝气生物滤池(Z-BAF)亚硝化的可行性,以及碱度和氨氮的比值对亚硝化作用的影响,并对比了Na_2CO_3和NaHCO_3作为碱度供体的经济成本。结果表明,废水经Z-BAF生化处理后,可实现氨氮的部分亚硝化,通过调节进水碱度能够控制氨氮的亚硝化率,使出水NO_2^--N/氨氮值稳定在1. 0～1. 4之间。以Na_2CO_3作为碱度的来源,采用两级Z-BAF分步投加碱度法,可以避免一次投加导致pH值过高问题,从而降低对生化反应的影响,并且使用Na_2CO_3代替常用的NaHCO_3作为碱度供体可节省约一半的成本。反应器稳定运行阶段,一级Z-BAF的亚硝态氮产率为0. 36 kg/(m^3·d),二级Z-BAF的亚硝态氮产率为0. 67 kg/(m^3·d),厌氧氨氧化反应器经过100 d运行后,总氮去除率可稳定在70%以上。

关键词：氧化铁红废水短程硝化厌氧氨氧化沸石曝气生物滤池

The Effects of Reclamation Activity and Yellow River Runoff on Coastline and Area of the Laizhou Bay,China

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Journal of Ocean University of China 2021年第3期20卷 729-739页

作者： XU Yandong GAO huiwang WEI xiao ZHU Jinlong Key Laboratory of Marine Environment and Ecology Ministry of EducationOcean University of ChinaQingdao 266100China Shandong Provincial Key Laboratory of Restoration for Marine Ecology Shandong Marine Resource and Environment Research InstituteYantai 264006China Laboratory for Marine Ecology and Environmental Science Pilot National Laboratory for Marine Science and Technology(Qingdao)Qingdao 266237China

Study on morphological changes of a bay can help to identify the effects of anthropogenic activities on coastal environ-ment and guide the exploration of marine *** this paper,morphological data including coastline and water areas in five discrete years between 1968 and 2015 were selected and extracted from the remote sensing images and historical marine charts to study the morphological changes in Laizhou Bay(LZB),one of the bays in the southwest of the Bohai Sea.A systematic analysis on spatial variations of the coastline and the surface areas of different types of waters in LZB was *** results showed that the surface area of LZB was decreased by 1253.2km^(2)in the last half century,which is 17.4%of the total in the *** areas of the natural wetland and the intertidal zone were decreased by 17.2%and 56.1%,respectively,and the average water depth varied from 9.05 m to 8.16m at low tide level from 1968 to *** coastline and shape variations of the bay turned to be complex after the 1980s,and the shape index of LZB showed an increasing trend in more recent *** centroid of the bay generally migrated to the northeast direction,i.e.,the direction of the center of the Bohai Sea,and the shrinking direction of the bay was consistent with the migration direction of the *** reclamation area during 1968-2015 in LZB was 1201.7km^(2),and 94.1%was in the inter-tidal *** overall morphological change of the bay during the last half century was mainly controlled by the coastal reclamation activities,and the Yellow River runoff including the river course change and sediment load variation was also an important control-ling factor.

关键词： morphological change coastal reclamation Yellow River runoff Laizhou Bay

Chill injury in the eggs of the migratory locust, Locusta migratoria （Orthoptera： Acrididae）： the time-temperature relationship with high-temperature interruption

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Insect Science 2005年第3期12卷 171-178页

作者： xiao-HONGJING XIAN-huiwang LEKANG State Key Laboratory of Integrated Management of Pest Insects and Rodents Institute of ZoologyChinese Academy of SciencesBeijingChina

Mortality of the overwintering egg of the migratory locust, Locustamigratoria L., was attributed to chill injury because of its occurrence well above the egg' ssupercooling point. In this study, two parameters, upper limit of chill injury zone (ULCIZ) and sumof the injurious temperature (SIT), were used to examine the locust egg's cold hardiness. The valueof ULCIZ for the locust egg is 1.06 ± 0.54℃, and the SIT is -329.7 (hour ? degree). Thesuperoxide dismutase (SOD) and catalase (CAT) activities changed dramatically after cold stress,indicating that oxygen and hydroxide free radicals are probably efficiently detoxified at lowtemperatures. It was suggested that the nature of chill injury in locust egg might be a complex ofmetabolic disorder and a non-proportional decrease in enzymatic reaction and transports, because theLDH activity at low temperature increased significantly and the ATPase activity decreased withprolonged duration of exposure to low temperatures. The results from high temperature interruptionrevealed that the high temperature intervals significantly increased the survival of locust eggs.

关键词： locusta migratoria chill injury antioxidant enzymes cold hardiness

Identification and characterization of cul-3b,a novel hominine CUL-3 transcript variant

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Asian Journal of Andrology 2005年第2期7卷 205-211页

作者： LiLu Zuo-MingZhou xiao-YanHuang MinXu Lan-LanYin huiwang Zhi-YangXu Jia-HaoSha KeyLaboratoryofReproductiveMedicine NanjingMedicalUniversityNanjing210029China

Aim:To identify genes related to the human testis development by substrate hybridization ***:A human testis cDNA microarray was constructed and hybridized with probes prepared from human adult and fetal testes and spermatozoa mRNAs by reverse transcription *** differentially expressed genes were sequenced. And a newly identified cullin-3 (CUL-3) transcript variant (designated cul-3b) was bio-informatically analyzed with an online GenBank ***-tissue reverse transcription polymerase chain reaction (RT-PCR) was used to deter- mine the tissue expression profile of ***:Cul-3b,a novel CUL-3 transcript variant,was *** expression level of cul-3b in adult testes was 3.79-fold higher than that in fetal ***-3b differed from cul-3 (including NM_003590 and AY337761) in the opening reading frame and had three internal ribosomal entry sites (IRESes) in the 5'-*** led to a 24 amino acid (aa) truncation at N-terminus of CUL-3b as compared with CUL-3 and a more motivated expression pattern of cul-3b under some strict ***,cul-3b expressed ubiquitously in human tissues according to multi-tissue ***:Cul-3b is a novel transcript variant of CUL-3,which may be important not only for the development of human testis but also for that of other organs.

关键词： alternative splicing CUL-3 DNA sequence human testis microarray

Seasonal and Spatial Variations of Macro Benthos in the Intertidal Mudflat of Southern Yellow River Delta,China in 2007/2008

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Journal of Ocean University of China 2018年第2期17卷 437-444页

作者： ZOU Li YAO xiao YAMAGUCHI Hitomi GUO Xinyu GAO huiwang WANG Kai SUN Mingyi Key Laboratory of Marine Environmental Science and Ecology Ministry of EducationOcean University of ChinaQingdao 266100China College of Environmental Science and Engineering Ocean University of ChinaQingdao 266100China Environmental Protection Bureau Dongying 257091China Faculty of Agriculture Kagawa University2393 IkenobeKitaMiki 7610795Japan Affiliation&Position National University Corporation Ehime University Ehime 79085775Japan Department of Marine Sciences University of GeorgiaAthens 30602-3636USA

In order to examine the seasonal and spatial distributions of benthic animals in the intertidal mudflat of the southern Yellow River Delta,field investigations were carried out in 2007 and 2008 and multiple methods were *** showed that,the biomass of macro benthos ranged at 0.75-1151.00 g wet m^(-2) and averaged at 156.31 g wet m^(-2),in which Mactra veneriformis accounted for 75.6%-93.4% of the total macro benthic *** than 90% of macro benthos inhabited in the middle and low tide lines,and higher biomass occurred in early summer and lower in *** analysis showed that:1)*** growth was primarily favored at higher temperature and lower salinity;2)after long time interaction,benthic bivalve grazers led to patching distributions of Chlorophyll a(Chl a);3)macro benthic biomass positively related with Chl a when the concentration of Chl a was low,but they were negatively related when Chl a concentration was high;and 4)furthermore,the biomass of benthic bivalves peaked in the sediment with median grain size about 0.55 mm,but decreased gradually in coarse or fine *** secondary productivity ranged at 0.37-283.68 g m^(-2)yr^(-1) and averaged at 47.88 g m^(-2) yr^(-1),in which 69.7% was contributed by *** It was estimated that primary production was transformed to secondary production at a rate of 6.87%approximately,which implies that there is a local sustainability of high bivalve production.

关键词： macro benthos Mactra veneriformis distribution intertidal mudflat southern Yellow River Delta

Identify lymphatic metastasis-associated genes in mouse hepatocarcinoma cell lines using gene chip

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World Journal of Gastroenterology 2005年第10期11卷 1463-1472页

作者： BoSong Jian-WuTang BoWang xiao-NanCui LiHou LuSun Li-MinMao Chun-HuiZhou YueDu Li-huiwang Hua-XinWang Ren-ShuZheng LeiSun DepartmentofPathology DalianMedicalUniversityDalian116027LiaoningProvinceChina ShanghaiJingtaiBiotechnologyCorporationLtd Shanghai200000China

AIM: In order to obtain lymphogenous metastasisassociated genes, we compared the transcriptional profiles of mouse hepatocarcinoma cell lines Hca-F with highly lymphatic metastasis potential and Hca-P with low lymphatic metastasis ***: Total RNA was isolated from Hca-F and Hca-P cells and synthesized into double-stranded cDNA. In vitro transcription double-stranded cDNA was labeled with biotin (i.e. biotin-labeled cRNA, used as the probe). The cRNA probes hybridized with Affymetrix GeneChip() MOE430A (containing 22 690 transcripts, including 14 500 known mouse genes and 4 371 ESTs) respectively and the signals were scanned by the GeneArray Scanner. The results were then analyzed by ***: Out of the 14 500 known genes investigated,110 (0.8%) were up regulated at least 23 fold. Among the total 4 371 ESTs, 17 ESTs (0.4%) (data were not presented) were up regulated at least 23 fold. According to the Gene Ontology and TreeView analysis, the 110genes were further classified into two groups: differential biological process profile and molecular function ***: Using high-throughput gene chip method,a large number of genes and their cellular functions about angiogenesis, cell adhesion, signal transduction, cell motility, transport, microtubule-based process, cytoskeleton organization and biogenesis, cell cycle, transcription,chaperone activity, motor activity, protein kinase activity,receptor binding and protein binding might be involved in the process of lymphatic metastasis and deserve to be used as potential candidates for further *** D1, Fosl1, Hsp47, EGFR and AR, and Cav-1 are selected as the possible candidate genes of the metastatic phenotype, which need to be validated in later *** (data were not presented) might indicate novel genes associated with lymphatic metastasis. Validating the function of these genes is helpful to identify the key or candidate gene/pathway responsible for lymphatic m

关键词： Hepatocarcinoma Lymphatic metastasis Cell lines Hca-F and Hca-P Gene chip

莱州湾表层沉积物中重金属污染特征和生态风险评估

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中国海洋大学学报（自然科学版） 2021年第11期51卷 74-85页

作者：徐艳东高会旺魏潇朱金龙中国海洋大学海洋环境与生态教育部重点实验室山东青岛266100 山东省海洋资源与环境研究院山东省海洋生态修复重点实验室山东烟台264006 青岛海洋科学与技术试点国家实验室海洋生态与环境科学功能实验室山东青岛266237

根据2015年8月莱州湾30个站位的表层沉积物中7种重金属调查资料,通过单因子污染指数、重金属污染负荷指数、潜在生态风险指数及主成分分析等4种方法,分析了莱州湾重金属含量空间污染分布特征和主要来源,评估了其生态风险,确定了关键重... 详细信息

根据2015年8月莱州湾30个站位的表层沉积物中7种重金属调查资料,通过单因子污染指数、重金属污染负荷指数、潜在生态风险指数及主成分分析等4种方法,分析了莱州湾重金属含量空间污染分布特征和主要来源,评估了其生态风险,确定了关键重金属污染要素,并探讨了关键污染要素的历史变化。结果表明:研究海域沉积物重金属中Cu、Pb、Zn、Cr、Cd、Hg和As含量平均值分别为14.0、16.1、50.6、36.5、0.2、0.034和10.4 mg/kg,其中Cd、Hg和As的含量存在不同数量的站位高于海洋沉积物第一类质量标准值和渤海沉积物背景值及山东省土壤背景值现象。与中国近海沉积物相比,莱州湾金属含量总体处于中等水平。重金属潜在生态风险由高到低依次为Hg>Cd>As>Pb>Cu>Cr>Zn,潜在生态风险高值区主要分布于湾的中西部、南部和东北部,其中Hg和Cd总贡献率最高。Hg、Cd和As为莱州湾表层沉积物中重金属污染的关键要素,它们的含量均高于1980年代的水平。

关键词：表层沉积物重金属生态风险关键污染要素莱州湾

重度子痫前期胎盘组织中sVCAM-1、GDF-15的表达及其临床意义

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中国现代医学杂志 2022年第17期32卷 67-72页

作者：刘艳秋马小艳贺龙风王荟江苏大学附属医院妇产科江苏镇江212002

目的探讨重度子痫前期(SPE)胎盘组织中可溶性血管内皮黏附分子-1(sVCAM-1)、生长分化因子-15(GDF-15)的表达及临床意义。方法选取2018年5月—2021年5月江苏大学附属医院行剖宫产术终止妊娠的204例妊娠高血压患者,其中单纯妊娠高血压患... 详细信息

目的探讨重度子痫前期(SPE)胎盘组织中可溶性血管内皮黏附分子-1(sVCAM-1)、生长分化因子-15(GDF-15)的表达及临床意义。方法选取2018年5月—2021年5月江苏大学附属医院行剖宫产术终止妊娠的204例妊娠高血压患者,其中单纯妊娠高血压患者87例(妊娠高血压组),子痫前期(PE)患者52例(PE组),SPE患者65例(SPE组),另选取同期该院产检住院行剖宫产分娩的健康妊娠孕妇52例为对照组。通过实时荧光定量聚合酶链反应(qRT-PCR)检测所有研究对象胎盘组织中sVCAM-1、GDF-15的表达。分析不同人群胎盘组织中sVCAM-1 mRNA、GDF-15 mRNA相对表达量,Pearson法分析sVCAM-1 mRNA与GDF-15 mRNA的相关性;多因素Logistic回归模型分析影响SPE发生的因素。结果对照组胎盘组织中sVCAM-1 mRNA、GDF-15 mRNA相对表达量低于妊娠高血压组、PE组及SPE组(P<0.05),妊娠高血压组sVCAM-1 mRNA、GDF-15 mRNA相对表达量低于PE组和SPE组(P<0.05),PE组sVCAM-1 mRNA、GDF-15 mRNA相对表达量均低于SPE组(P<0.05)。Pearson相关性分析结果显示,SPE组sVCAM-1 mRNA与GDF-15 mRNA呈正相关(P<0.05)。多因素Logistic回归分析结果显示,sVCAM-1 mRNA[O^R=3.550(95%CI:1.461,8.628)]、GDF-15 mRNA[O^R=4.059(95%CI:1.670,9.865)]是SPE发生的影响因素(P<0.05)。结论胎盘组织中sVCAM-1、GDF-15的异常表达可能与PE发病有关,sVCAM-1、GDF-15高表达可能使PE患者进展为SPE。

关键词：重度子痫前期胎盘可溶性血管内皮黏附分子-1 生长分化因子-15