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Determinants of lexical access in pure-anomic recovery:a longitudinal study

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2009年第5期10卷 341-347页

作者： xiao zhou hui LIANG Ming-wei XU Ben-yan LUO Neuropsychology Laboratory the First Affiliated Hospital School of Medicine Zhejiang University Hangzhou 310003 China

Many studies involving lexical access in picture-naming tasks have been undertaken at a point in time,mainly focusing on age of acquisition(AoA).To identify the real determinates of lexical access in recovery and their traces in the brain,we carried out a longitudinal study on a Chinese pure anomic patient using multiple logistic regression *** found that AoA played an important role in early recovery but not in total recovery,whereas familiarity was significant in the whole *** a new dynamic point of view,our results indicate that AoA and familiarity are the main determinants of lexical access in anomia *** suggest that the changing effects of AoA during recovery may be related to the pathologic process;AoA and familiarity should be taken into account in constructing materials to assess and treat anomic patients.

关键词： Object naming Age of acquisition Anomia Therapy

Senescent mesenchymal stem/stromal cells and restoring their cellular functions

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World Journal of Stem Cells 2020年第9期12卷 966-985页

作者： Qing-Shu Meng Jing Liu Lu Wei hui-Min Fan xiao-hui zhou xiao-Ting Liang Shanghai Heart Failure Research Center Shanghai East HospitalTongji University School of MedicineShanghai 200120China Research Center for Translational Medicine Shanghai East HospitalTongji University School of MedicineShanghai 200120China Institute of Integrated Traditional Chinese and Western Medicine for Cardiovascular Chronic Diseases Tongji University School of MedicineShanghai 200120China Department of Heart Failure Shanghai East HospitalTongji University School of MedicineShanghai 200120China Institute for Regenerative Medicine Shanghai East HospitalSchool of Life Sciences and TechnologyTongji UniversityShanghai 200120China

Mesenchymal stem/stromal cells(MSCs)have various properties that make them promising candidates for stem cell-based therapies in clinical *** include self-renewal,multilineage differentiation,and ***,recent studies have confirmed that aging is a vital factor that limits their function and therapeutic properties as standardized clinical *** the features of senescence and exploration of cell rejuvenation methods are necessary to develop effective strategies that can overcome the shortage and instability of *** review will summarize the current knowledge on characteristics and functional changes of aged ***,it will highlight cell rejuvenation strategies such as molecular regulation,noncoding RNA modifications,and microenvironment controls that may enhance the therapeutic potential of MSCs in clinical settings.

关键词： Mesenchymal stem cells Senescence Features Function Rejuvenation strategy

A Confocal Technique Applicable to Studies of Cellular pH-related Signaling in Plants

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Journal of Integrative Plant Biology 2008年第6期50卷 682-690页

作者： Bing-Bing Lit Zhi-hui Gao xiao-Yan zhou hui-Bo Ren Min Xie Yi-Juan Fan Jian-Fang Hu Wen-Suo Jia College of Agronomy and Biotechnology China Agricultural University Beijing 100094 China

pH may act as a crucial signal in both animal and plant cells. It is very difficult to monitor pH signals and this has largely hindered progress in the investigation of pH signaling, particulady systematic pH signaling. Here, we report the development of a confocal technique to monitor leaf apoplastic pH in intact plants, which is particularly suitable for the studies on root to shoot signaling. A variety of different pH indicators and plant species were tested. It was found that different pH indicators, for example, 2＇,7＇-Bis-（2-carboxyethyl）-5-（and-6）-carboxyfluoresce （BCECF）, SNARF-4F 5-（and-6）- carboxylic acid （SNARF） and DM-NERF （NERF）, were of different properties, and to successfully monitor pH at a sub-cellular level, the comparability between the pH indicator and plant species must be involved according to their suitable pH range and loading characteristics. The loading characteristics of different pH indicators differ with different plant species, cell types and their developing stages. No matter what methods were adopted, BCECF and SNARF could not be loaded specifically in the leaf apoplast in sunflower, tomato, and Comelina communis L. In contrast, regardless of the methods adopted, NERF could be loaded efficiently and specifically in the leaf apoplast in C. communis, but not in other plants. In C. communis, the determination coefficient for in vitro and in situ calibration of NERF was very high, which was respectively 0.9951 and 0.991 6, and therefore, the adoption of NERF together with C. communis could construct an ideal experimental system that is suitable for the investigation of pH systematic signaling. Ratio image analysis demonstrated that the leaf apoplastic pH was about 5.5 in non-stressed conditions, and water deficit could trigger an increase in pH by about half a pH unit, which is the first evidence to directly indicate that pH is able to act as a systematic signal under water deficit conditions.

关键词： confocal microscope pH signaling water deficit.

High persistence rate of hepatitis B virus in a hydrodynamic injection-based transfection model in C3H/He N mice

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World Journal of Gastroenterology 2015年第12期21卷 3527-3536页

作者： Xiu-Hua Peng xiao-Nan Ren Li-Xiang Chen Bi-Sheng Shi Chun-Hua Xu Zhong Fang Xue Liu Jie-Liang Chen xiao-Nan Zhang Yun-Wen Hu xiao-hui zhou Shanghai Public Health Clinical Center Key Laboratory of Medical Molecular Virology Ministry of Education and Health Fudan University

AIM: To optimize the viral persistence rate in a hydrodynamic injection(HI) based hepatitis B virus(HBV) transfection mouse ***:(1) 5-6-wk-old male C3H/He N and C57BL/6 mice were hydrodynamically injected with 10 μg endotoxin-free p AAV/HBV1.2 plasmid DNA via the tail vein. Hepatitis B surface antigen(HBs Ag), hepatitis B e antigen(HBe Ag) and HBV DNA, both in the serum and liver, were detected at different time points post HI by ELISA, immunohistochemical staining or quantitative polymerase chain reaction(PCR);(2) male C3H/He N and C57BL/6 mice, either hydrodynamically injected mice at 10 wk post HI or na?ve mice, were all immunized subcutaneously with 5 μg HBs Ag formulated in complete Freund's adjuvant three times at a 2-wk interval. Two weeks after the final immunization, splenocytes were isolated for T cell function analysis by ELISPOT assay; and(3) five weeks post HI, C3H/He N mice were intragastrically administered 0.1 mg/kg entecavir once a day for 14 d, or were intraperitoneally injected with 1 mg/kg interferon(IFN)-α twice a week for 2 wk, or were treated with PBS as controls. The sera were collected and assayed for HBV DNA on days 0, 7 and 14 after drug treatment. RESULTS:(1) Approximately 90%(22/25) of the injected C3H/He N mice were still HBs Ag-positive at 46 wk post HI, whereas HBs Ag in C57BL/6 mice were completely cleared at 24 wk. Serum levels of HBe Ag in C3H/He N mice were higher than those in C57BL/6 mice from 4 wk to 46 wk. HBV DNA levels in the hydrodynamically injected C3H/He N mice were higher than those in the C57BL/6 mice, both in the serum(from 4 wk to 46 wk) and in the liver(detected at 8 wk and 46 wk post HI). Histology showed that hepatitis B core antigen and HBs Ag were expressed longer in the liver of C3H/He N mice than in C57BL/6;(2) HBs Ag specific T cell responses after HBs Ag vaccination in hydrodynamically injected C3H/He N and C57BL/6 mice, or naive control mice were detected by ELISPOT assay. After stimulation with H

关键词： Hepatitis B virus Hydrodynamic injection Viral per

Human liver chimeric mouse model based on diphtheria toxin-induced liver injury

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World Journal of Gastroenterology 2017年第27期23卷 4935-4941页

作者： xiao-Nan Ren Rong-Rong Ren Hua Yang Bo-Yin Qin Xiu-Hua Peng Li-Xiang Chen Shun Li Meng-Jiao Yuan Chao Wang xiao-hui zhou Shanghai Public Health Clinical Center Fudan University Key Laboratory of Medical Molecular Virology Ministry of Education and HealthFudan University

AIM To establish an inducible liver injury mouse model and transplant human hepatocytes to obtain liverhumanized *** We crossed three mouse strains,including albumin(Alb)-cre transgenic mice,inducible diphtheria toxin receptor(DTR) transgenic mice and severe combined immune deficient(SCID)-beige mice,to create Alb-cre/DTR/SCID-beige(ADSB) mice,which coincidentally harbor Alb-cre and DTR transgenes and are immunodeficient. As the Cre expression is driven by the liver-specific promoter Alb(encoding ALB),the DTR stop signal flanked by two lox P sites can be deleted in the ADSB mice,resulting in DTR expression in the liver. ADSB mice aged 8-10 wk were injected intraperitoneally(i.p.) with diphtheria toxin(DT) and liver damage was assessed by serum alanine aminotransferase(ALT) level. Two days later,mouse livers were sampled for histological analysis,and human hepatocytes were transplanted into the livers on the same day. A human ALB enzyme-linked immunosorbent assay was performed 7,14,21 and 28 d after transplantation. Human CD68 immunohistochemistry was performed 30 and 90 d after *** We crossed Alb-cre with DTR and SCID-beige mice to obtain ADSB mice. These mice were found to have liver damage 4 d after i.p. injection of 2.5 ng/g bodyweight DT. Bodyweight began to decrease on day 2,increased on day 7,and was lowest on day 4(range,10.5%-13.4%). Serum ALT activity began to increase on day 2 and reached a peak value of 289.7 ± 16.2 IU/m L on day 4,then returned to background values on day 7. After transplantation of human liver cells,peripheral blood human ALB level was 1580 ± 454.8 ng/m L(range,750.2-3064.9 ng/m L) after 28 d and Kupffer cells were present in the liver at 30 d in ADSB *** Human hepatocytes were successfully repopulated in the livers of ADSB mice. The inducible mouse model of humanized liver in ADSB mice may have functional applications,such as hepatocyte transplantation,hepatic regeneration and drug metabolism.

关键词： Liver disease Liver injury Diphtheria toxin Liver chimeric mouse model

Overview of the reporter genes and reporter mouse models

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Animal Models and Experimental Medicine 2018年第1期1卷 29-35页

作者： Shun Li Li-xiang Chen Xiu-hua Peng Chao Wang Bo-yin Qin Dan Tan Cheng-xiao Han Hua Yang xiao-nan Ren Fang Liu Chun-hua Xu xiao-hui zhou Key Laboratory of Medical Molecular Virology Shanghai Public Health Clinical Center Ministry of Education and HealthFudan University School of Agriculture and Biology Shanghai Jiao Tong University

Reporter genes are widely applied in biotechnology and biomedical research owning to their easy observation and lack of toxicity. Taking advantage of the reporter genes in conjunction with imaging technologies, a large number of reporter mouse models have been generated. Reporter mouse models provide systems that enable the studies of live cell imaging, cell lineage tracing, immunological research and cancers etc. in vivo. In this review, we describe the types of different reporter genes and reporter mouse models including, random reporter strains, Cre reporter strains and ROSA26 reporter strains. Collectively, these reporter mouse models have broadened scientific inquires and provided potential strategies for generation of novel reporter animal models with enhanced capabilities.

关键词： Cre reporter strains random reporter strains reporter genes ROSA26 reporter strains

Development of a humanized HLA-A30 transgenic mouse model

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Animal Models and Experimental Medicine 2022年第4期5卷 350-361页

作者： Meng-min Zhu Bo-wen Niu Ling-ling Liu Hua Yang Bo-yin Qin Xiu-hua Peng Li-xiang Chen Yang Liu Chao Wang xiao-nan Ren Chun-hua Xu xiao-hui zhou Feng Li Department of Laboratory Animal Science Shanghai Public Health Clinical CenterShanghaiChina

Background:There are remarkable genetic differences between animal major histocompatibility complex(MHC)systems and the human leukocyte antigen(HLA)*** transgenic humanized mouse model systems offer a much better method to study the HLA-A-related principal mechanisms for vaccine development and HLA-Arestricted responses against infection in ***:A recombinant gene encoding the chimeric HLA-A30 monochain was *** HHD molecule contains the following:α1-α2 domains of HLA-A30,α3 and cytoplasmic domains of H-2D~b,linked at its N-terminus to the C-terminus of humanβ2m by a 15-amino-acid peptide *** recombinant gene encoding the chimeric HLA-A30 monochain cassette was introduced into bacterial artificial chromosome(BAC)CH502-67J3 containing the HLA-A01 gene locus by Red-mediated homologous *** BAC CH502-67J3 was microinjected into the pronuclei of wild-type mouse *** humanized mouse model was further used to assess the immune responses against influenza A virus(H1N1)pdm09 clinically isolated from human *** cell population,cytokine production,and histopathology in the lung were ***:We describe a novel humanβ2m-HLA-A30(α1α2)-H-2D~b(α3 transmembrane cytoplasmic)(HHD)monochain transgenic mouse strain,which contains the intact HLA-A01 gene locus including 49 kb 5’-UTR and 74 kb 3’-UTR of HLA-A01**** transgenic lines integrated into the large genomic region of HLA-A gene locus were obtained,and the robust expression of exogenous transgene was detected in various tissues from A30-18#and A30-19#lines encompassing the intact flanking *** cytometry revealed that the introduction of a large genomic region in HLA-A gene locus can influence the immune cell constitution in humanized ***09 infection caused a similar immune response among HLA-A30 Tg humanized mice and wild-type mice,and induced the rapid increase of cytokines,including IFN-γ,TNF-α,and IL-6,in both HLA-A30 humanized

关键词： HLA-A30 humanized mouse immunology major histocompatibility complex(MHC)

Induced pluripotent stem cell-related approaches to generate dopaminergic neurons for Parkinson's disease

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Neural Regeneration Research 2025年第11期20卷 3193-3206页

作者： Ling-xiao Yi hui Ren Woon Genevieve Saw Li Zeng Eng King Tan Zhi Dong zhou National Neuroscience Institute Singapore Nanyang Technology University Singapore Department of Neurology Singapore General HospitalSingapore Signature Research Program in Neuroscience and Behavioral Disorders Duke-NUS Medical SchoolSingapore

The progressive loss of dopaminergic neurons in affected patient brains is one of the pathological features of Parkinson's disease,the second most common human neurodegenerative *** the detailed pathogenesis accounting for dopaminergic neuron degeneration in Parkinson's disease is still unclear,the advancement of stem cell approaches has shown promise for Parkinson's disease research and *** induced pluripotent stem cells have been commonly used to generate dopaminergic neurons,which has provided valuable insights to improve our understanding of Parkinson's disease pathogenesis and contributed to anti-Parkinson's disease *** current review discusses the practical approaches and potential applications of induced pluripotent stem cell techniques for generating and differentiating dopaminergic neurons from induced pluripotent stem *** benefits of induced pluripotent stem cell-based research are *** dopaminergic neuron differentiation protocols from induced pluripotent stem cells are *** emerging three-dimension-based brain organoid models compared with conventional two-dimensional cell culture are ***,limitations,challenges,and future directions of induced pluripotent stem cell–based approaches are analyzed and proposed,which will be significant to the future application of induced pluripotent stem cell-related techniques for Parkinson's disease.

关键词： dopaminergic neurons induced pluripotent stem cells Parkinson's disease stem cell approaches

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PVD涂层刀具切削砂岩的磨损面积研究

组合机床与自动化加工技术 2017年第9期 59-61,65页

作者：杨小军周慧包头钢铁职业技术学院内蒙古包头014010

研究切削参数对PVD涂层刀具磨损面积的影响。利用正交试验进行切削试验,采用网格划分方法计算刀具磨损面积,分析刀具磨损面积与主轴转速、进给速度和切削深度之间的关系,根据试验结果得到:提高主轴转速能够降低刀具磨损面积,增加进给速... 详细信息

研究切削参数对PVD涂层刀具磨损面积的影响。利用正交试验进行切削试验,采用网格划分方法计算刀具磨损面积,分析刀具磨损面积与主轴转速、进给速度和切削深度之间的关系,根据试验结果得到:提高主轴转速能够降低刀具磨损面积,增加进给速度与切削深度将使刀具磨损面积增大。根据经验公式,利用最小二乘法建立刀具磨损面积预测模型,通过F检验可知模型具有较高的显著性,并得到切削参数中影响刀具磨损面积的主次关系为切削深度、主轴转速、进给速度。

关键词：刀具磨损面积切削参数网格划分方法