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Identification of a Resistance Gene bls1 to Bacterial Leaf Streak in Wild Rice Oryza rufipogon Griff

Journal of Integrative Agriculture 2012年第6期11卷 962-969页

作者： HE Wen-ai HUANG Da-hui LI Rong-bai QIU Yong-fu SONG Jian-dong YANG Hai-ning ZHENG Jia-xing HUANG Yue-yue LI xiao-qiong LIU Chi ZHANG Yue-xiong MA Zeng-feng YANG Yong Guangxi University Library Guangxi University Nanning 530004 P.R.China Guangxi Crop Genetic Improvement and Biotechnology Laboratory/Rice Research Institute Nanning 530007 P.R. China State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources China Agricultural College Guangxi University Nanning 530004 P.R.China Guangxi Academy of Agricultural Sciences Guangxi University Nanning 530004 P.R

Bacterial leaf streak （BLS） of rice, caused by Xanthomonas oryzae pv. oryzicola （Xoc） is a worldwide destructive disease. Development of resistant varieties is considered to be one of the most effective and eco-friendly ways to control the disease. However, only a few genes/QTLs having resistance to BLS have been identified in rice until now. In the present study, we have identified and primarily mapped a BLS-resistance gene, blsl, from a rice line DP3, derived from the wild rice species Oryza rufipogon Griff. A BC2F2 （9311/DP3//9311） population was constructed to map BLS-resistance gene in the rice line DP3. The segregation of the resistant and susceptible plants in BCzFz in 1：3 ratio （Z2=0.009, Z20 05,1=3.84, P〉0.05）, suggested that a recessive gene confers BLS resistance in DP3. In bulked segregant analysis （BSA）, two SSR markers RM8116 and RM584 were identified to be polymorphic in resistant and susceptible DNA bulks. For further mapping the resistance gene, six polymorphic markers around the target region were applied to analyze the genotypes of the BC2F2 individuals. As a result, the BLS-resistant gene, designated as blsl, was mapped in a 4.0-cM region flanked by RM587 and RM510 on chromosome 6.

关键词： bacterial leaf streak Xanthomonas oryzae pv. oryzicola Oryza rufipogon Griff. gene mapping marker-assisted selection (MAS)

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Process mineralogy of copper-nickel sulphide flotation by a cyclonic-static micro-bubble flotation column

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Mining Science and Technology 2009年第6期19卷 784-787页

作者： CAO Yi-jun GUI Xia-hui MA Zi-long YU xiao-xia CHEN xiao-dong ZHANG Xiu-pin School of Chemical Engineering and Technology China University of Mining ＆ Technology Xuzhou Jiangsu 221008 China Jinchuan Group Ltd. Jinchang Gansu 737100 China

In our study we investigated a refractory copper-nickel sulfide ore separation by using a cyclonic-static micro-bubble flotation column (FCSMC). The process mineralogy of the main products was studied. Using a scanning electron microscope-energy dispersive system (SEM-EDS) and an X-ray spectrometer the mineral category and content of samples were analyzed. By using a mineral liberation analyzer (MLA) the mineral liberation characteristics were revealed. It is shown that in roughing feed the monomers liberation degree of nickel pyrite and chalcopyrite take up 84.11% and 88.82%, respectively. In tailings, the lost nickel pyrite and chalcopyrite are mainly monomers. Therefore, strengthening the micro-fine particle recovery capacity is the key to increase recovery.

关键词： cyclonic-static micro-bubble flotation column process mineralogy copper-nickel sulphide micro-fine particles

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A molecular half-adder and half-subtractor based on pyrylium

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Chinese Science Bulletin 2010年第25期55卷 2799-2802页

作者： CAO ChaoTun LIU hui ZHU xiao-Qing CHENG Jin-Pei Department of Chemistry State Key Laboratory of Elemento-Organic Chemistry Nankai University Tianjin 300071 China

A very simple molecular cation, 4-(4-dimethylaminophenyl)-2,6-diphenylpyrylium, has been demonstrated to have a function of molecular half-adder and half-subtractor according to the detectable spectroscopic changes of the molecular system in response to the inputs of acid and base. Distinct algebraic operations can be performed in this reconfigurable molecular logic system.

关键词：分子离子半加器减法吡喃基础逻辑系统氨基苯基功能变化

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Preparation and characterization of HMX/EVA/hBNNSs microcomposites with improved thermal stability and reduced sensitivity

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Defence Technology（防务技术） 2021年第2期17卷 650-656页

作者： Yue Yang xiao-dong Li Yan-tao Sun Jian-an Tian hui-min Liu Bi-dong Wu Jing-yu Wang School of Environment and Safety Engineering North University of ChinaTaiyuan030051ShanxiChina Shanxi Engineering Technology Research Center for Ultrafine Powder North University of ChinaTaiyuan030051ShanxiChina

Hexagonal boron nitride nanosheets(HBNNSs)have huge potential in the field of coating materials owing to their remarkable chemical stability,mechanical strength and thermal ***-layer hBNNSs were obtained by a liquid-phase exfoliation of h-BN powders and incorporated into EVA coatings for improving the safety performance of 1,3,5,7-tetranitro-1,3,5,7-tetrazocane(HMX).HBNNSs and ethylene-vinyl acetate copolymer(EVA)were introduced to HMX by a solvent-slurry *** comparison,the HMX/EVA and HMX/EVA/graphene(HMX/EVA/G)composites were also prepared by a similar *** morphology,crystal form,surface element distribution,thermal decomposition property and impact sensitivity of HMX/EVA/hBNNSs composites were contrastively *** showed that as prepared HMX/EVA/hBNNSs composites were well coated with hBNNSs and EVA,and exhibited better thermal stability and lower impact sensitivity than that of HMX/EVA and HMX/EVA/G composites,suggesting superior performance of desensitization of hBNNSs in explosives.

关键词： Energetic materials 2D(two dimensional)materials HBNNSs(hexagonal boron nitride nanosheets) HMX(1,3,5,7-Tetranitro-1,3,5,7-tetrazocane) Thermal stability Mechanical sensitivity

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基于序贯分析策略的丹红注射液与临床常用输液溶媒配伍稳定性及安全性评价

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药物分析杂志 2024年第3期44卷 493-500页

作者：王安婧朱越韩诗晴韩柱诸葛慧贾小转王迎超杨振中浙江大学药学院杭州310058 浙江大学智能创新药物研究院杭州310018

目的:基于“理化性质-主要成分含量-指纹图谱相似度-类过敏反应”的序贯分析策略,评价丹红注射液与临床常用输液溶媒0.9%氯化钠注射液和5%葡萄糖注射液在不同配伍条件下溶液的稳定性及安全性。方法:根据临床使用情况,将丹红注射液与0.9... 详细信息

目的:基于“理化性质-主要成分含量-指纹图谱相似度-类过敏反应”的序贯分析策略,评价丹红注射液与临床常用输液溶媒0.9%氯化钠注射液和5%葡萄糖注射液在不同配伍条件下溶液的稳定性及安全性。方法:根据临床使用情况,将丹红注射液与0.9%氯化钠注射液或5%葡萄糖注射液分别按20 mL:100 mL、30 mL:100 mL、40 mL:100 mL和30 mL:250 mL、40 mL:250 mL的比例进行配伍,在室温条件下10 h内,分别观察和检测各溶液在配伍后不同时间点的性状、渗透压、不溶性微粒和pH;测定溶液在配伍后不同时间点的各主要成分含量;计算配伍后不同时间点各溶液HPLC指纹图谱和^(1)H NMR指纹图谱的相似度;采用RBL-2H3细胞脱颗粒体外类过敏反应检测体系,评价各配伍溶液的安全性。结果:配伍10 h内溶液性状、渗透压、不溶性微粒含量、pH无明显变化,主要成分含量无显著差异,HPLC和1H NMR指纹图谱均有良好的相似度,且配伍溶液无明显致RBL-2H3细胞脱颗粒作用。结论:本研究从物理、化学、生物角度综合评价,表明在30 mL:250 mL~40 mL:100 mL浓度范围内,丹红注射液与0.9%氯化钠注射液或5%葡萄糖注射液在配伍10 h内溶液均有良好的稳定性和安全性,可为临床使用提供参考。

关键词：丹红注射液溶媒配伍含量测定指纹图谱类过敏反应序贯分析稳定性研究安全性评价

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气候因子对呼伦贝尔沙化草地生态系统健康性的影响

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生态与农村环境学报 2024年第9期40卷 1191-1200页

作者：图雅王百竹王丹雨朱媛君杨晓晖 Indree Tuvshintogtokh 王百田北京林业大学水土保持学院北京100083 中国林业科学研究院荒漠化研究所/生态保护与修复研究所北京100091 蒙古国科学院植物园与植物研究所蒙古国乌兰巴托13330

健康的生态系统对于提供人类所需的服务至关重要,并具有巨大的社会和经济价值,如何评价生态系统健康性具有重要意义。研究基于群落特征和土壤理化性质各项指标计算呼伦贝尔沙化草地生态系统健康性指数(CVOR)及其准则层的4项组成指数(草... 详细信息

健康的生态系统对于提供人类所需的服务至关重要,并具有巨大的社会和经济价值,如何评价生态系统健康性具有重要意义。研究基于群落特征和土壤理化性质各项指标计算呼伦贝尔沙化草地生态系统健康性指数(CVOR)及其准则层的4项组成指数(草地基况指数C、活力指数V、组织力指数O及恢复力指数R),通过Fisher有序样本聚类法对CVOR指数进行等级划分。结果表明:呼伦贝尔沙化草地生态系统健康性等级共分为4级:1级健康,2级亚健康,3级警戒,4级崩溃;CVOR指数及其准则层4项组成指数与气候因子在不同健康等级样地之间存在显著差异(P<0.05);年平均温度和生长季平均温度与各项健康指数呈显著负相关关系,年平均降水量和生长季降水量与各项指数呈显著正相关关系(P<0.05)。这表明降水量的增加有利于提高呼伦贝尔沙化草地生态系统健康性,而温度升高将不利于维持草地生态系统健康性。在未来全球气候变暖的趋势下,呼伦贝尔沙化草地生态系统健康性存在逐年降低的风险,草场管理人员可以通过降低草场利用强度以及其他管理方式来维持草地生态系统健康性的可持续发展。

关键词：生态系统健康性呼伦贝尔草原 CVOR指数气候变化可持续发展

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Role of exercise training on insulin resistance and TNF-α in high-fat diet rats

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Frontiers of Medicine 2009年第4期3卷 403-407页

作者： hui SUN Xiuling DENG Fangxi xiao Lulu CHEN huiqing LI Department of Endocrinology Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430022china

This study investigated the effect of exercise training on insulin resistance and serum and adipose TNF-αin high-fat diet-induced insulin-resistant *** male Wistar rats were randomly divided into two groups:normal control group(NC;n=8)that accepted normal chow and high-fat diet group(HF;n=22)that fed on high-fat diet to induce insulin resistance *** HF group was randomly assigned to two subgroups after 18 weeks:sedentary group(SE;n=10)and exercise training group(ET;n=12)that performed swimming exercise training for 6 weeks,while both groups continued high-fat *** of body weight,lipid proﬁle,and fasting plasma glucose and insulin were *** insulin sensitivity index(ISI)was *** concentration of TNF-αwas detected by *** expression of TNF-αmRNA and protein in adipose tissue was examined by using real-timeﬂuorescence quantitative polymerase chain reaction(PCR)and Western blot,*** 18 weeks,compared with the NC group,body weight,blood lipid,glucose,and insulin in the HF group were signiﬁcantly elevated,while the ISI decreased obviously,which suggested that insulin resistance appeared in the HF *** exercise training for 6 weeks,compared with the SE group,both ISI and serum TNF-αconcentration in the ET group were decreased signiﬁcantly;however,the expression levels of TNF-αmRNA and protein in adipose tissue increased by 27.5%and 20.5%,*** conclusion,exercise training ameliorates insulin *** reduction of the level of serum TNF-αand the increased expression of TNF-αin adipose tissue by exercise training may be involved in this mechanism.

关键词： exercise training insulin resistance tumor necrosis factor-α

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Endogenous hydrogen sulfide and ERK1/2-STAT3 signaling pathway may participate in the association between homocysteine and hypertension

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Journal of Geriatric Cardiology 2019年第11期16卷 822-834页

作者： Lin SHI xiao-Yun LIU Zhi-Gang HUANG Zhi-Yi MA Yang XI Lu-Yan WANG ning-Ling SUN Hypertension Research Laboratory Department of CardiologyPeking University People’s HospitalBeijingChina Emergency Department Peking University Shenzhen HospitalShenzhenChina

Background Homocysteine(Hcy)is a risk factor for hypertension,although the mechanisms are poorly *** We first explored the relationship between Hcy levels and blood pressure(BP)by analyzing the clinical data of primary hypertensive patients admitted to our ***,we explored a rat model to study the effect of Hcy on blood pressure and the role of *** hyperhomocysteinemia(HHcy)rat model was induced to explore the effect of Hcy on blood pressure and the possible *** carried out tissue histology,extraction and examination of RNA and ***,we conducted cell experiments to determine a likely mechanism through renin-angiotensin-aldosterone system(RAAS)and extracellular signal-regulated kinase 1/2(ERK1/2)signaling *** In primary hypertensive inpatients with HHcy,blood pressure was significantly higher as compared with inpatient counterparts lacking *** the rat model,blood pressure of the Wistar rats was significantly increased with increases in serum Hcy levels and decreased after folate *** converting enzyme 1(ACE1)expression in the Wistar Hcy group was enhanced comparing to controls,but was decreased in the Wistar folate *** II receptor type 1(AGTR1)levels in the kidney tissue increased in the Wistar folate *** serum H2S and kidney cystathionineγ-lyase decreased with elevated levels of serum *** vitro,increased concentrations and treatment times for Hcy were associated with increased expression of collagen type 1 and *** dose and time dependent response was also observed for p-STAT3 and p-ERK1/2 *** Endogenous H2S might mediate the process of altered blood pressure in response to changes in serum Hcy levels,in a process that is partly dependent on activated RAAS and ERK1/2-STAT3 signaling pathway.

关键词： Angiotensin converting enzyme 1 Blood pressure ERK1/2-STAT3 signaling pathway Homocysteine Hydrogen sulfide

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猫泛白细胞减少症病毒YX01株的分离鉴定及致病性分析

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动物医学进展 2024年第9期45卷 12-19页

作者：马建伟付展鸿钟林翰韩言言王以欣姜晓霞孙晓波王美宋厚辉徐义刚李园浙江农林大学动物科技学院/动物医学院

用F81细胞从疑似感染猫泛白细胞减少症病毒(FPV)猫的组织样本中分离出1株FPV毒株，命名为FPV YX01株，通过电镜观察、血凝试验以及化学试剂(20%乙醚和0.1 mol/L HCl)处理，分析了FPV YX01株的生物学特性；基于VP2基因序列，分析了FPV Y... 详细信息

用F81细胞从疑似感染猫泛白细胞减少症病毒(FPV)猫的组织样本中分离出1株FPV毒株，命名为FPV YX01株，通过电镜观察、血凝试验以及化学试剂(20%乙醚和0.1 mol/L HCl)处理，分析了FPV YX01株的生物学特性；基于VP2基因序列，分析了FPV YX01株的遗传进化，通过动物回归试验评价了该分离毒株的致病力。结果显示，FPV YX01毒株感染F81细胞后，细胞出现萎缩拉丝、脱落、生长缓慢等典型病变；电镜观察可见FPV YX01病毒粒子呈圆形，表面无囊膜结构，直径约25 nm,可被FPV特异性抗体识别；遗传进化分析发现，YX01毒株VP2蛋白中决定FPV血凝活性和毒力的关键氨基酸位点未发生突变，且YX01毒株与意大利EU498695毒株高度同源；动物回归试验显示，感染YX01毒株可引起猫出现食欲减退、腹泻、体温双相热、肠胀气、肝脏出血以及白细胞数量急剧减少等猫泛白细胞减少症的典型临床症状，同时病理切片可见肝脏和肠组织出血、炎性细胞浸润等现象。研究结果分离到1株具有较强致病力的FPV毒株YX01株，为FPV调查、检测、试剂研发及抗体制备提供了物质基础。

关键词：猫泛白细胞减少症病毒分离鉴定生物学特性致病性

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Prevention of carbon migration in 9% Cr/CrMoV dissimilar welded joint by adding tungsten inert gas overlaying layer

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Journal of Iron and Steel Research International 2018年第8期25卷 847-853页

作者： Kai Ding hui-jun Ji Xia Liu Peng Wang Quan-liang Zhang xiao-hong Li Yu-lai Gao State Key Laboratory of Advanced Special Steel Shanghai Key Laboratory of Advanced Ferrometallurgy School of Materials Science and Engineering Shanghai University Shanghai 200072 China Shanghai Electric Power Generation Equipment Co. Ltd.Shanghai Turbine Plant Shanghai 200240 China

High chromium （9-12% Cr） steels with excellent heat resistance and CrMoV steels with good toughness were potential candidates for combined rotor for steam turbine operated over 620℃. Two welding techniques were used to fabricate 9% Cr and CrMoV dissimilar welded joint. The results show that the carbon migration only appears in the specimen using narrow gap submerged arc welding （NG-SAW） technique, yet it can be effectively prevented by adding tungsten inert gas （TlG） overlaying process before the NG-SAW. The carbon migration occurred in NG-SAW resulting from the sharp transition of the strong carbide-forming element Cr between the weld （-2.7 wt%） and the base metal （- 9 wt%）. On the contrary, the application of TIG overlaying layers can promote the diffusion of Cr element, and therefore result in its much smaller concentration gradient. That is to say, a gentle transition zone of Cr element can be created among the SAW weld, TIG overlaying layers and the base metal, which effectively prevents the carbon migration and therefore produces a decreased carbon concentration adjacent to the fusion line.

关键词： Carbon migration Tungsten inert gas overlaying Gentle transition zone Narrow gap submerged arc welding Dissimilar welded joint

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