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Diversity of culturable heterotrophic bacteria from the Mariana Trench and their ability to degrade macromolecules

Marine Life Science & Technology 2020年第2期2卷 181-193页

作者： Xiuxiu Zhao Jiwen Liu Shun Zhou Yanfen Zheng Yanhong Wu Kazuhiro Kogure xiao-hua zhang MOE Key Laboratory of Marine Genetics and Breeding College of Marine Life SciencesOcean University of China.Qingdao 266003China Laboratory for Marine Ecology and Environmental Science Pilot National Laboratory for Marine Science and Technology(Qingdao)Qingdao 266237China Institute of Evolution and Marine Biodiversity Ocean University of ChinaQingdao 266003China Atmosphere and Ocean Research Institute The University of Tokyo5-1-5.Kashiwanoha.KashiwaChiba 277-8564Japan

The Mariana Trench is the deepest location on earth and harbors unique microbial communities as evidenced by 16S rRNA gene amplicon and metagenomic *** culturable microorganisms from the Mariana Trench will contribute to a further understanding of hadal biogeochemical processes and act as a unique microbial reservoir with potential ***,825 bacterial strains,identified by 16S rRNA gene sequencing,were isolated from 12 water depths(0-10,400 m)of the Mariana Trench with 2216E and R2A media at 4℃ or 28℃ on four cruises during *** bacteria belong to four phyla,nine classes,27 orders,45 families and 108 ***,Gammaproteobacteria,Actinobacteria_c,Bacilli and Flavobacteriia were the most abundant classes,accounting for 37.9%,33.0%,11.8%,8.6%and 8.0%of the total bacterial isolates,respectively.2216E and R2A media were found to have a better selectivity to Bacilli and Flavobacteriia,*** strains were potential novel bacterial species with a 16S rRNA gene similarity<98.65%,and a higher percentage of novel strains were obtained from R2A than 2216E ***,301(150 species)out of 354 strains(178 species)selected from each depth could degrade at least one of the ten kinds of macromolecules *** results indicate that there is a high diversity of culturable bacteria in the Mariana Trench and they can produce a variety of extracellular *** study provides a valuable resource of microorganisms for investigating their biogeochemical roles in the Mariana Trench and for industrial applications.

关键词： Diversity Heterotrophic bacteria The Mariana Trench Macromolecule Water column

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Caspase-1 inhibition alleviates acute renal injury in rats with severe acute pancreatitis

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World Journal of Gastroenterology 2014年第30期20卷 10457-10463页

作者： xiao-hua zhang Min-Li Li Bin Wang Mei-Xia Guo Ren-Min Zhu Department of Gastroenterology Jinling Hospital

AIM: To assess the effect of inhibition of caspase-1 on acute renal injury in rats with severe acute pancreatitis (SAP).

关键词： Severe acute pancreatitis Caspase-1 Interleukin-1 beta Interleukin-18 Acute renal injury

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Isolation and Characterization of a Dichlorvos-Degrading Strain DDV-1 of Ochrobactrum sp.

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Pedosphere 2006年第1期16卷 64-71页

作者： zhang xiao-hua zhang Guo-Shun zhang Zhong-Hui XU Jian-Hong LI Shun-Peng Key Lab of Microbiological Engineering of Agricultural Environment Ministry of Agriculture Nanjing Agricultural University Nanjing 210095 （China）. Jiangsu-Polytechnic College of Agriculture and Forestry Jurong 212400 （China）

The objective of this research was to isolate a dichlorvos (2,2-dichlorovinyl dimethyl phosphate)-degrading strain of Ochrobactrum sp., and determine its effectiveness in remediation of a dichlorvos-contaminated soil. A dichlorvos-degrading bacterium (strain DDV-1) was successfully isolated and identified as an Ochrobactrumsp. based on its 16S rDNA sequence analysis. Strain DDV-1 was able to utilize dichlorvos as a sole carbon source, and the optimal pH and temperature for its cell growth and degradation were 7.0 and 30 ℃, respectively. Also, the growth and degradation of strain DDV-1 showed the same response to dissolved oxygen. In addition, the soil degradation test indicated that in soil spiked with 100 mg L-1 or 500 mg L-1 dichlorvos and inoculated with 0.5% or 1.0% (v/v) strain DDV-1, complete degradation of dichlorvos could be achieved in 24 h. The present study showed that strain DDV-1 was a fast dichlorvos-degrading bacterium in soil. However, further research will be needed to clarify the degradation pathway and the properties of the key enzymes involved in its biodegradation.

关键词： degradation dichlorvos 16S rDNA soil test strain DDV-1

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Phylotype resolved spatial variation and association patterns of planktonic Thaumarchaeota in eastern Chinese marginal seas

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Marine Life Science & Technology 2023年第2期5卷 257-270页

作者： Jiwen Liu Fuyan huang Jiao Liu xiaoyue Liu Ruiyun Lin xiaosong Zhong Brian Austin xiao-hua zhang Frontiers Science Center for Deep Ocean Multispheres and Earth System and College of Marine Life SciencesOcean University of ChinaQingdao 266100China Laboratory for Marine Ecology and Environmental Science Laoshan LaboratoryQingdao 266237China Institute of Evolution and Marine Biodiversity Ocean University of ChinaQingdao 266003China Key Laboratory of Marine Chemistry Theory and Technology Ministry of EducationQingdao 266100China Institute of Aquaculture University of StirlingStirling FK94LAScotlandUK

The majority of marine ammonia oxidizers belong to Thaumarchaeota,a phylum of Archaea,which is distributed throughout the water *** surface waters contain distinct thaumarchaeotal phylotypes compared to the deeper ocean,but spatial dynamics of the surface-associated lineages are largely *** study of 120 seawater samples from the eastern Chinese marginal seas identifed contrasting distribution and association patterns among thaumarchaeotal phylotypes across diferent ***,Nitrosopumilus-like and Nitrosopelagicus-like phylotypes dominated the surface water(3 m)of the Yellow Sea(YS)and East China Sea(ECS),respectively,along with increased abundance of total free-living Thaumarchaeota in *** compositional changes were observed in the surface *** spatial heterogeneity of particle-attached Thaumarchaeota was less clear in surface microlayers than in surface ***,the Nitrosopelagicus-like phylotype increased in abundance from surface to 90 m in ECS,which led to an increase in the proportion of Thaumarchaeota relative to total *** occurred mainly in the free-living *** results indicate a clear size-fractionated niche partitioning,which is more pronounced at lower depths than in the surface water/surface *** addition,associations of Thaumarchaeota with other microbial taxa varied between phylotypes and size *** results show that a phylotype-resolved and size-fractionated spatial heterogeneity of the thaumarchaeotal community is present in surface oceanic waters and a vertical variation of the Nitrosopelagicus-like phylotype is present in shallow shelf waters.

关键词： Thaumarchaeota Phylotype Association pattern Spatial variation Surface microlayer Chinese marginal seas

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Effects of ω-3 fatty acids on toll-like receptor 4 and nuclear factor-κB p56 in lungs of rats with severe acute pancreatitis

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World Journal of Gastroenterology 2016年第44期22卷 9784-9793页

作者： Bin Wang xiao-Wei Wu Mei-Xia Guo Min-Li Li xiao-Bing Xu Xin-Xin Jin xiao-hua zhang Department of Gastroenterology Jinling Hospital

AIM To determine the effects of ω-3 fatty acids(ω-3FA) on the toll-like receptor 4(TLR4)/nuclear factor κB p56(NF-κBp56) signal pathway in the lungs of rats with severe acute pancreatitis(SAP).METHODS A total of 56 Sprague-Dawley rats were randomly divided into 4 groups: control group, SAP-saline group, SAP-soybean oil group and SAP-ω-3FA group. SAP was induced by the retrograde infusion of sodium taurocholate into the pancreatic duct. The expression of TLR4 and NF-κBp56 in the lungs was evaluated by immunohistochemistry and Western blot analysis. The levels of inflammatory cytokines interleukin-6 and tumor necrosis factor-alpha in the lungs were measured by enzyme-linked immunosorbent assay. RESULTS The expression of TLR4 and NF-κBp56 in lungs and of inflammatory cytokines in serum significantly increased in the SAP group compared with the control group(P < 0.05), but was significantly decreased in the ω-3FA group compared with the soybean oil group at 12 and 24 h(P < 0.05).CONCLUSION During the initial stage of SAP, ω-3FA can efficiently lower the inflammatory response and reduce lung injury by triggering the TLR4/NF-κBp56 signal pathway.

关键词： Severe acute pancreatitis ω-3 fatty acids Lung injury Toll-like receptor 4 Nuclear factor-κB p56 Cytokine

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Cadmium Accumulation and Translocation in Two Jerusalem Artichoke(Helianthus tuberosus L.) Cultivars

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Pedosphere 2011年第5期21卷 573-580页

作者： CHEN Liang LONG xiao-hua zhang Zhen-hua ZHENG xiao-Tao Z. RENGEL LIU Zhao-Pu Jiangsu Provincial Key Laboratory of Marine Biology College of Resources and Environmental Sciences Nanjing Agricultural University Nanjing 210095 (China) Soil Science and Plant Nutrition School of Earth and Environment the University of Western Australia 35 Stirling Highway Crawley WA 6009 (Australia)

Jerusalem artichoke(Helianthus tuberosus L.) not just can be used for bioethanol production but may be potentially used in phytoremediation for the removal of heavy metal *** Jerusalem artichoke cultivars,N2 and N5,were subjected to six cadmium(Cd) concentrations(0,5,25,50,100 and 200 mg L1) to investigate Cd tolerance and *** 21 days of growth,the effects of Cd on growth,chlorophyll content,net photosynthetic rate,intercellular CO2 concentration and malondialdehyde content were *** growth parameters were reduced under Cd *** two Jerusalem artichoke cultivars had relatively high Cd tolerance and accumulation capacity(> 100 mg kg1),with N5 being more tolerant and having higher Cd accumulation than *** accumulated more Cd than stems and *** bioconcentration factors(far higher than 1) and translocation factors(lower than 1) decreased with an increase in Cd *** results suggested that Jerusalem artichoke could be grown at relatively high Cd loads,and N5 could be an excellent candidate for phytoremediation of Cd-contaminated soils.

关键词： bioconcentration factor Cd-contaminated soils heavy metal phytoremediation tolerance index

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Endoscopic submucosal dissection vs endoscopic mucosal resection for colorectal tumors: A meta-analysis

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World Journal of Gastroenterology 2014年第25期20卷 8282-8287页

作者： Jing Wang xiao-hua zhang Jian Ge Chong-Mei Yang Ji-Yong Liu Shu-Lei Zhao Department of Gastroenterology Shandong Provincial Hospital Affiliated to Shandong University

AIM: To compare the efficacy and safety of endoscopic submucosal dissection (ESD) and endoscopic mucosal resection (EMR) for the treatment of colorectal tumors.

关键词： Endoscopic submucosal dissection Endoscopic mucosal resection Colorectal tumors En bloc resection Local recurrence Histological resection Complication

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Vertical variation in Vibrio community composition in Sansha Yongle Blue Hole and its ability to degrade macromolecules

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Marine Life Science & Technology 2020年第1期2卷 60-72页

作者： Bei Li Jiwen Liu Shun Zhou Liang Fu Peng Yao Lin Chen Zuosheng Yang xiaolei Wang xiao-hua zhang College of Marine Life Sciences Ocean University of China5 Yushan RoadQingdao 266003China Laboratory for Marine Ecology and Environmental Science Qingdao National Laboratory for Marine Science and TechnologyQingdao 266237China Sansha Track Ocean Coral Reef Conservation Research Institute Sansha 573199China Key Laboratory of Marine Chemistry Theory and Technology Ministry of Education/Institute for Advanced Ocean StudyOcean University of ChinaQingdao 266100China College of Marine Geosciences Ocean University of China.Qingdao 266100China Institute of Evolution and Marine Biodiversity Ocean University of ChinaQingdao 266003China

With the advantages of wide distribution,fast growth,and broad metabolic spectrum to organic carbon compounds,Vibrio may play an important role in organic carbon ***,the ecological roles of Vibrio in many marine environments have not been ***,the world's deepest'blue hole',the Sansha Yongle Blue Hole(SYBH)in the South China Sea,which is a geographically semi-enclosed environment featuring unique chemical characters,was *** abundance,diversity and carbon source utilization capability of Vibrio were studied by quantification and high-throughput sequencing of Vibrio specific 16S rRNA genes and cultivation *** abundance of Vibrio in water column of the SYBH ranged from 3.78×10^4 to 7.35×10^616S rRNA gene copies L_***-living Vibrio was more abundant than particle-associated Vibrio(~1.20×10^6 versus-2.68×10^5 gene copies L_1),indicating that Vibrio prefers a free-living life *** Vibrio assemblages showed clear vertical stratification and could be divided into three groups:aerobic-transition,middle anaerobic and bottom anaerobic *** oxygen(DO),temperature,pH and salinity were the main environmental factors affecting the abundance and community *** Vibrio demonstrated a degrading capability to various macromolecular substrates,including starch,Tween 20/40/80,DNA,gelatin,alginate,casein,chitin,lecithin,K-carrageenan,mannan,xylan and hyaluronic *** suggests that Vibrio could produce a variety of highly active extracellular *** study provides new insights into the distribution pattern and possible role in carbon cycle of Vibrio in the unique environment of a"blue hole'.

关键词： Vibrio community Vertical stratification Carbon source utilization Sansha Yongle Blue Hole

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Biogenic production of DMSP and its degradation to DMS—their roles in the global sulfur cycle

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Science China(Life Sciences) 2019年第10期62卷 1296-1319页

作者： xiao-hua zhang Ji Liu Jingli Liu Guipeng Yang Chun-Xu Xue Andrew R. J. Curson Jonathan D. Todd MOE Key Laboratory of Marine Genetics and Breeding College of Marine Life SciencesOcean University of ChinaQingdao 266003China Laboratory for Marine Ecology and Environmental Science Qingdao National Laboratory for Marine Science and TechnologyQingdao 266237China College of Chemistry and Chemical Engineering Ocean University of ChinaQingdao 266071China School of Biological Sciences University of East AngliaNorwich NR4 7TJUK

Dimethyl sulfide(DMS) is the most abundant form of volatile sulfur in Earth’s oceans, and is mainly produced by the enzymatic clevage of dimethylsulfoniopropionate(DMSP). DMS and DMSP play important roles in driving the global sulfur cycle and may affect climate. DMSP is proposed to serve as an osmolyte, a grazing deterrent, a signaling molecule, an antioxidant, a cryoprotectant and/or as a sink for excess sulfur. It was long believed that only marine eukaryotes such as phytoplankton produce DMSP. However, we recently discovered that marine heterotrophic bacteria can also produce DMSP, making them a potentially important source of DMSP. At present, one prokaryotic and two eukaryotic DMSP synthesis enzymes have been *** heterotrophic bacteria are likely the major degraders of DMSP, using two known pathways: demethylation and *** phytoplankton and some fungi can also cleave DMSP. So far seven different prokaryotic and one eukaryotic DMSP lyases have been identified. This review describes the global distribution pattern of DMSP and DMS, the known genes for biosynthesis and cleavage of DMSP, and the physiological and ecological functions of these important organosulfur molecules, which will improve understanding of the mechanisms of DMSP and DMS production and their roles in the environment.

关键词： marine microorganism dimethylsulfoniopropionate (DMSP) dimethyl sulfide (DMS) DMSP biosynthesis DMSP cleavage

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Deep Brain Stimulation of the Globus Pallidus Internus in Patients with Intractable Tourette Syndrome： A 1-year Follow-up Study

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Chinese Medical Journal 2016年第9期129卷 1022-1027页

作者： xiao-hua zhang Jian-Yu Li Yu-Qing zhang Yong-Jie Li Department of Functional Neurosurgery Beijing Institute of Functional Neurosurgery Xuanwu Hospital Capital Medical University Beijing 100053 China

Background： Deep brain stimulation （DBS） has been a promising treatment for patients with refractory Tourette syndrome （TS） for more than a decade. Despite successful DBS treatment of TS in more than 100 patients worldwide, studies with a large patient sample and long-term follow-up assessments are still scarce. Accordingly, we investigated the clinical efficacy and safety of globus pallidus internus （GPi） DBS in the treatment of intractable TS in 24 patients with a 1-year follow-up assessment. Methods： Bilateral/unilateral GPi-DBS was performed in 24 patients with TS. We evaluated symptoms of tics and obsessive-compulsive disorder （OCD） through the Yale Global Tic Severity Scale （YGTSS） and Yale-Brown Obsessive-compulsive Scale （Y-BOCS）. We used the Wechsler Adult Intelligence Scale-Revised in China （WAIS-RC） to evaluate the safety of the treatment. We conducted follow-up assessments of all patients for at least 12 months （12-99 months）. Results： Symptoms of tics and OCD were significantly relieved at a 12-month follow-up assessment. The mean YGTSS score was 74.04 ± 11.52, 49.83 ± 10.91, 32.58 ± 7.97, and 31.21 ± 8.87 at baseline, 3, 6, and 12 months, respectively. The mean YGTSS scores obtained at the follow-up assessments were significantly different from the baseline （P 〈 0.05）. The improvement in motor tics was superior to that in phonic tics. The mean Y-BOCS scores were 21.61 ± 4.97, 18 ± 4.58, 14.39 ± 3.99, and 13.78 ± 4.56 at baseline, 3, 6, and 12 months, respectively （P 〈 0.05）. We observed a remarkable improvement in psychiatric comorbidities, such as OCD and attention-deficit hyperactivity disorder, after the procedure. WAIS-RC scores were comparable before and after the operation. There were no severe postoperative complications. Conclusion： GPi-DBS appears to comprehensively alleviate tic symptoms and psychiatric comorbidities in patients with TS, thus significantly improving patients＇ quality of life.

关键词： Deep Brain Stimulation Tourette Syndrome Treatment

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