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rAd-p53 enhances the sensitivity of human gastric cancer cells to chemotherapy

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World Journal of Gastroenterology 2011年第38期17卷 4289-4297页

作者： Guang-Xia Chen Li-Hong Zheng Shi-Yu Liu xiao-hua he Department of Gastroenterology First People＇s Hospital of Xuzhou Xuzhou 221002 Jiangsu Province China Yantai Economic and Technological Development Zone Hospital Yantai 264006 Shandong Province China

AIM:To investigate potential antitumor effects of rAd-p53 by determining if it enhanced sensitivity of gastric cancer cells to chemotherapy.METHODS:Three gastric cancer cell lines with distinct levels of differentiation were treated with various doses of rAd-p53 alone,oxaliplatin(OXA) alone,or a combination of both.Cell growth was assessed with an 3-(4,5)-dimethylthiahiazo(-z-y1)-3,5-diphenytetrazoliumromide assay and the expression levels of p53,Bax and Bcl-2 were determined by immunohistochemistry.The presence of apoptosis and the expression of caspase-3 were determined using flow cytometry.RESULTS:Treatment with rAd-p53 or OXA alone inhibited gastric cancer cell growth in a time-and dosedependent manner;moreover,significant synergistic effects were observed when these treatments were combined.Immunohistochemical analysis demonstrated that treatment with rAd-p53 alone,OXA alone or combined treatment led to decreased Bcl-2 expression and increased Bax expression in gastric cancer cells.Furthermore,flow cytometry showed that rAd-p53 alone,OXA alone or combination treatment induced apoptosis of gastric cancer cells,which was accompanied by increased expression of caspase-3.CONCLUSION:rAd-p53 enhances the sensitivity of gastric cancer cells to chemotherapy by promoting apoptosis.Thus,our results suggest that p53 gene therapy combined with chemotherapy represents a novel avenue for gastric cancer treatment.

关键词：胃癌细胞化疗药物 RAD P53 敏感性 caspase 免疫组化法流式细胞仪

SYNTheSIS,SELF-ASSEMBLY AND RESPONSIVE PROPERTIES OF PEG-b-PDMAEMA-b-PMMAzo TRIBLOCK COPOLYMERS

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Chinese Journal of Polymer Science 2013年第5期31卷 833-840页

作者： Yao-hui Xing 林绍梁林嘉平 xiao-hua he Shanghai Key Laboratory of Advanced Polymeric Materials Key Laboratory for Ultrafine Materials of Ministry of EducationSchool of Materials Science and EngineeringEast China University of Science and Technology Department of Chemistry East China Normal University

A novel amphiphilic copolymer poly（ethylene glycol）-block-poly（N,N-dimethylamino-2-ethylmethacrylate）-block- poly[6-（4-methoxy-azobenzene-4＇-oxy） hexyl methacrylate] （PEG-b-PDMAEMA-b-PMMAzo） was prepared by ATRP polymerization. The self-assembly and responsive behaviors were investigated by SEM, TEM, LLS and UV-Vis spectra. The results indicated that the copolymers can self-assemble into spherical structures in aqueous media. The aggregate size can be tuned by pH and temperature. The trans-cis isomerization behavior of the formed aggregates was also examined. Upon irradiation with a linear polarized light, the elongation degree of the aggregates was increased with the irradiation time.

关键词： zobenezene Copolymer Self-assemble Responsive.

IL-1β: an important cytokine associated with febrile seizures?

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Neuroscience Bulletin 2012年第3期28卷 301-308页

作者： Hong-Mei Yu Wan-Hong Liu xiao-hua he Bi-Wen Peng Department of Physiology School of Basic Medical SciencesWuhan UniversityWuhan 430071China

Febrile seizures （FSs） are the most common convulsions in childhood. Studies have demonstrated a significant relationship between a history of prolonged FSs during early childhood and temporal sclerosis, which is responsible for intractable mesial temporal lobe epilepsy. It has been shown that interleukin-1β （IL-1β） is intrinsically involved in the febrile response in children and in the generation of FSs. We summarize the gene polymorphisms, changes of IL-1β levels and the putative role of IL-1β in the generation of FSs. IL-1β could play a role either in enhancing or in reducing neural excitability. If the enhancing and reducing effects are balanced, an FS does not occur. When the enhancing effect plays the leading role, an FS is generated. A mild imbalance can cause simple FSs while a severe imbalance can cause complex FSs and febrile status epilepticus. Therefore, anti-IL-1β therapy may help to treat FSs.

关键词： febrile seizures IL-1β cytokines gene polymorphism

Dysfunction of hippocampal interneurons in epilepsy

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Neuroscience Bulletin 2014年第6期30卷 985-998页

作者： Yu-Qiang Liu Fang Yu Wan-Hong Liu xiao-hua he Bi-Wen Peng Department of Physiology School of Basic Medical SciencesWuhan University Department of Pathology Zhongnan Hospital of Wuhan University Department of Immunology School of Basic Medical SciencesWuhan University Department of Pathophysiology School of Basic Medical SciencesWuhan University

Gamma-amino-butyric acid（GABA）-containing interneurons are crucial to both development and function of the brain. Down-regulation of GABAergic inhibition may result in the generation of epileptiform activity. Loss, axonal sprouting, and dysfunction of interneurons are regarded as mechanisms involved in epileptogenesis. Recent evidence suggests that network connectivity and the properties of interneurons are responsible for excitatory-inhibitory neuronal circuits. The balance between excitation and inhibition in CA1 neuronal circuitry is considerably altered during epileptic changes. This review discusses interneuron diversity, the causes of interneuron dysfunction in epilepsy, and the possibility of using GABAergic neuronal progenitors for the treatment of epilepsy.

关键词： interneuron diversity therapy GABA progenitor cell

Experimental characterization of anisotropic tensile mechanical behavior of pure titanium tube

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Journal of Iron and Steel Research(International) 2019年第1期26卷 91-101页

作者： Jin-biao Zhou Peng-yan Sun Chang-yu Zhou xiao-hua he School of Mechanical and Power Engineering Nanjing Technology UniversityNanjing 211816JiangsuChina

The anisotropic tensile mechanical properties of pure titanium(TA2)tubes were studied by means of theoretical analysis,finite element simulation and tensile tests.Based on the stress analysis of circumferential tensile of TA2 tube,an equation for calculating the tangential force of circumferential tensile specimen was established,and the relationship among tangential force,friction and position of ring specimen was analyzed.The finite element simulation analysis of circumferential tensile process of TA2 ring specimen was carried out,which verified the tangential force equation derived from theoretical analysis.The effect of gauge length and friction on necking and tensile load was analyzed,and the optimal gauge length was selected for the ring hoop tensile test.Finally,the axial and circumferential tensile digital image correlation experiments were carried out to verify the theoretical and finite element simulation results.The friction coefficient between TA2 tube and D-blocks(using Teflon lubricant)was determined by the inverse finite element method and the friction experiment,and the true stress-strain curve of TA2 tube was obtained.The results show that the axial and circumfere ntial mechanical behaviors of TA2 tubes are significantly different and an isotropic.

关键词： Pure titanium(TA2)tube Ring hoop tensile test Digital image correlation An isotropy Strain rate

Orientation and strain rate dependent tensile behavior of single crystal titanium nanowires by molecular dynamics simulations

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Journal of Materials Science & Technology 2018年第5期34卷 864-877页

作者： Le Chang Chang-Yu Zhou Hong-Xi Liu Jian Li xiao-hua he School of Mechanical and Power Engineering Nanjing Tech University Nanjing 211816 China School of Mechanical Engineering Jiangsu University of Science and Technology Zhenjiang 212000 China

Molecular dynamics simulation was employed to study the tensile behavior of single crystal titanium nanowires（NWs）with[112^-0],[1^-100] and[0001]orientations at different strain rates from 10^8s^-1 to 10^11s^-1.When strain rates are above 10^10s^-1,the state transformation from HCP structure to amorphous state leads to super plasticity of Ti NWs,which is similar to FCC NWs.When strain rates are below 10^10s^-1,deformation mechanisms of Ti NWs show strong dependence on orientation.For [112^-0] orientated NW.{101^-1} compression twins（CTs）and the frequently activated transformation between CTs and deformation faults lead to higher plasticity than the other two orientated NWs.Besides,tensile deformation process along [112^-0] orientation is insensitive to strain rate.For [1^-100] orientated NW,prismaticslip is the main deformation mode at 10^8s^-1.As the strain rate increases,more types of dislocations are activated during plastic deformation process.For[0001]orientated NW,{101^-2} extension twinning is the main deformation mechanism,inducing the yield stress of [0001] orientated NW,which has the highest strain rate sensitivity.The number of initial nucleated twins increases while the saturation twin volume fraction decreases nonlinearly with increasing strain rate.

关键词： Molecular dynamics Single crystal titanium nanowires Strain rate Orientation Plastic deformation mechanisms

Effect of isothermal temper embrittlement and subsequent hydrogen embrittlement on tensile properties of 2.25Cr-1Mo-0.25V base metal and welded metal

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Journal of Iron and Steel Research(International) 2021年第11期28卷 1426-1438页

作者： Zhi-peng Shen Wei Fu Ling-rui Kong Han-han Ma xiao-hua he xiao-chun Yu Chang-yu Zhou School of Mechanical and Power Engineering Nanjing Tech UniversityNanjing 211816JiangsuChina Jiangsu Key Lab of Design and Manufacture of Extreme Pressure Equipment Nanjing 211816JiangsuChina

2.25Cr–1Mo–0.25V base metal(BM)and welded metal(WM)with different temper embrittlement states were obtained by isothermal temper embrittlement test.The ductile–brittle transition temperature and the carbide size of temper embrittled 2.25Cr–1Mo–0.25V BM and WM increased with the isothermal tempering time.The increase in temper embrittlement time leads to a decrease in yield strength(YS)and ultimate tensile strength(UTS).Hydrogen embrittlement(he)can decrease the ductility and increase YS and UTS of the material.The hydrogen embrittlement sensitivity and microstructure analysis both show a combined effect of he and temper embrittlement.The deeper the temper embrittlement,the more sensitive the material to he.When the hydrogen content in the material is low,the WM is less susceptible to he due to its welding defects.

关键词： 2.25Cr–1Mo–0.25V base metal Temper embrittlement Hydrogen embrittlement Mechanical property Combined effect

Complement C3 Aggravates Post-epileptic Neuronal Injury Via Activation of TRPV1

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Neuroscience Bulletin 2021年第10期37卷 1427-1440页

作者： Guang-Tong Jiang Lin Shao Shuo Kong Meng-Liu Zeng Jing-Jing Cheng Tao-Xiang Chen Song Han Jun Yin Wan-Hong Liu xiao-hua he Yu-Min Liu Lanzi Gongga Bi-Wen Peng Department of Physiology Hubei Provincial Key Laboratory of Developmentally Originated DiseaseSchool of Basic Medical SciencesWuhan UniversityWuhan 430071China Department of Pathophysiology School of Basic Medical SciencesWuhan UniversityWuhan 430071China Department of Immunology School of Basic Medical SciencesWuhan UniversityWuhan 430071China Department of Neurology Zhongnan HospitalWuhan UniversityWuhan 430071China Medical College Tibet UniversityLhasa 850013China

Epilepsy is a brain condition characterized by the recurrence of unprovoked seizures.Recent studies have shown that complement component 3(C3)aggravate the neuronal injury in epilepsy.And our previous studies revealed that TRPV1(transient receptor potential vanilloid type 1)is involved in epilepsy.Whether complement C3 regulation of neuronal injury is related to the activation of TRPV1 during epilepsy is not fully understood.We found that in a mouse model of status epilepticus(SE),complement C3 derived from astrocytes was increased and aggravated neuronal injury,and that TRPV 1-knockout rescued neurons from the injury induced by complement C3.Circular RNAs are abundant in the brain,and the reduction of circRad52 caused by complement C3 promoted the expression of TRPV 1 and exacerbated neuronal injury.Mechanistically,disorders of neuron-glia interaction mediated by the C3-TRPV1 signaling pathway may be important for the induction of neuronal injury.This study provides support for the hypothesis that the C3-TRFV1 pathway is involved in the prevention and treatment of neuronal injury and cognitive disorders.

关键词： TRPV 1 Complement C3 Epilepsy CircRad52 Cognitive disorder

Vortex identification based on the Liutex method and its effect on fish passage upstream

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Journal of Hydrodynamics 2024年第1期36卷 130-141页

作者： Chun-ying Shen Rui-guo Yang xiao-tao Shi Ming-ming Wang Shi-hua he Faculty of Electrical Power Engineering Kunming University of Science and TechnologyKunming650500China Hubei International Science and Technology Cooperation Base of Fish Passage China Three Gorges UniversityYichang443002China Shanghai Investigation Design and Research Institute Co.LtShanghai200434China

Fishway research is important for mitigating the fragmentation of river habitats caused by hydraulic projects.The vertical slit fishway is a broadly used fishway type because of its high efficiency and adaptability to water levels.However,the resulting vortex current disrupts the fish passage hence directly affecting fish migration.This study aims to accurately capture the vortex structure in the fishway and analyze the effect of vortex elements(vortex structure,vortex intensity,etc.)on fish.We conducted an analysis of the 3-D current flow field in the fishway through the utilization of an experimental model and the large eddy simulation(LES)method.Moreover,we captured the vortex information in the fishway at different flow rates using the Liutex vortex identification method and investigated the effect of the vortex on fish migration.The results revealed that the structures inside the fishway pool occupy most of the room,however,the areas with higher vortex strength were primarily located in the vortex near the vertical seam and the mainstream,the vortex strength inside the fishway gradually increases with increasing flow,suppressing fish migration.Fish experienced significantly increased resistance when encountering strong vortices.This suggests that the vortex may act as a physical barrier to fish migration.These findings highlight the potential negative effects of vortex on fish movement and reiterate the importance of understanding vortex dynamics for aquatic environmental management.As an effective tool for identifying vortices in fluid flow,the Liutex method demonstrates features of vortex within the fishway,thereby providing important insights into the interaction between fluid dynamics and aquatic organisms.

关键词： Vertical slit fishway vortex identification method Liutex large eddy simulation(LES) fish experiment computational fluid dynamics