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Molecular Characterization and Expression Analysis of TaZFP15, a C_2H_2-Type Zinc Finger Transcription Factor Gene in Wheat (Triticum aestivum L.)

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Journal of Integrative Agriculture 2012年第1期11卷 31-42页

作者： SUN Zhao-hua ding Chang-huan LI xiao-juan xiao Kai College of Life Science Agricultural University ofHebei Baoding 071001 P.R.China College of Agronomy Agrieultural University of Hebei Baoding 071001 P.R. China

Based on sequencing of part clones in a root subtractive cDNA library, an expressed sequence tag （EST） sharing high similarity to a rice C2H2 zinc finger transcription factor （ZFP15） was obtained in wheat. Through bioinformatics approach, the wheat C2H2-type ZFP gene referred to TaZFP15 has been identified and characterized. As a full-length cDNA of 670 bp, TaZFP15 has an open reading frame of 408 bp and encodes a 135-aa polypeptide. TaZFP15 contains two C2H2 zinc finger domains and each one has a conserved motif QALGGH. The typical L-box, generally identified in the C2H2 type transcription factors, has also been found in TaZFP15. Phylogenetic analysis suggested that TaZFP15 shares high similarities with rice ZFP15 （GenBank accession no. AY286473）, maize ZFP （GenBank accession no. NM_001159094） and a subset of other zinc-finger transcription factor genes in plant species. The expression of TaZFP15 was up-regulated by starved-Pi stress, showing a pattern to be gradually elevated along with the progression of the Pi-stress in a 23-h treatment regime. Similarly, the transcripts of TaZFP15 in roots were also induced by nitrogen deficiency, and abiotic stresses of drought and salinity. No responses of TaZFP15 were detected in roots to nutrition deficiencies of P, Zn, and Ca, and the external treatment of abscisic acid （ABA）. TaZFP15 could be specifically amplified in genome A, B, and D, and without variability in the sequences, suggesting that TaZFP15 has multi-copies in the homologous hexaploid species. Transgenic analysis in tobacco revealed that up-regulation of TaZFP15 could significantly improve plant dry mass accumulation via increasing the plant phosphorus acquisition capacity under Pi-deficiency condition. The results suggested that TaZFP15 is involved in mediation of signal transductions of diverse external stresses.

关键词： Triticum aestivum L. zinc-finger transcription factor gene nutrition deficiency abiotic stress expression pattern

Functional monodisperse microspheres fabricated by solvothermal precipitation co-polymerization

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Chinese Journal of Chemical Engineering 2021年第6期34卷 323-331页

作者： Fenghao Guo Yuanyuan ding Yanyan Wang xiao Gao Zhiyong Chen Shandong Provincial Key Laboratory of Fluorine Chemistry and Chemical Materials School of Chemistry and Chemical EngineeringUniversity of JinanJinan 250022China

Simultaneous achievement in high solid content and high microsphere yield is deemed a challenge in the fabrication of monodisperse microspheres by precipitation *** herein demonstrate that micro-sized monodisperse poly(methacrylic monomer-divinylbenzene)microspheres containing epoxy,lauyl,carboxyl and hydroxyl functions can be fabricated by solvothermal precipitation copolymerization at 20%(mass)monomer loading with over 94%microsphere *** morphology and porosity of the obtained particles can be readily tuned by cosolvent-acetonitrile binary *** of a small amount of cosolvent that has similar solubility parameter to that of the functional monomer can significantly improve the monodispersity of the obtained *** tetrahydrofuran was used as the co-solvent,the surface area of the highly porous microspheres achieved higher than 400 m^(2)·g^(-1).Solvothermal precipitation co-polymerization can be expected in scale-up fabrication of various monodisperse functional microspheres free of any surfactant and additive.

关键词： Monodisperse microsphere Solvothermal Methacrylic monomer Precipitation polymerization Core-shell structure

Antimicrobial power of biosynthesized Ag nanoparticles using refined Ginkgo biloba leaf extracts

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Frontiers of Materials Science 2022年第2期16卷 109-118页

作者： Wenbo ZhuANG Dafeng hu Xudong ZHANG Kai XIONG xiao ding Jian LU Yong MAO Peng YANG Chao LIU Yanfen WAN National Center for International Research on Photoelectric and Energy Materials Advanced Computing CenterMaterials Genome InstituteSchool of Materials and EnergyYunnan UniversityKunming 650091China Kunming Institute of Botany Chinese Academy of SciencesKunming 650201China Department of Nuclear Medicine Yunnan Cancer Hospital&The Third Affiliated Hospital of Kunming Medical UniversityKunming 650118China

Silver nanoparticles(Ag NPs),relative to existing antibacterial agents,are more effective,less toxic and more economical,and have shown enormous potential for the nanomedicine *** this work,we report a‘green’method for the rapid and efficient synthesis of Ag NPs using Ginkgo biloba extracts as reducing agent and capping *** properties of Ag NPs against fungi and bacteria were *** results showed that the Ginkgo biloba extracts are crucial for the preparation of uniform and monodispersed Ag *** prepared Ag NPs exhibited remarkable antibacterial *** minimum inhibitory concentrations of Ag NPs for Escherichia coli and Pseudomonas aeruginosa were 0.044 and 0.088μg·mL−1,***,Ag NPs exhibited excellent bactericidal performance against MDR-Pseudomonas *** was found that the effect of the antibacterial activity of Ag NPs on Escherichia coli and Staphylococcus aureus was tightly related to the reactive oxygen species *** research provides guidelines for the efficient green synthesis of Ag NPs and its antibacterial applications.

关键词： Ginkgo biloba extract silver nanoparticle green synthesis antibacterial application

Effects of encoding and retrieval on the mechanism of item+context binding

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Chinese Science Bulletin 2011年第17期56卷 1787-1798页

作者： xiao Xin ding JinHong GUO ChunYan Beijing Key Laboratory of Learning and Cognition and Department of Psychology Capital Normal University Beijing 100089 China

To investigate the neural mechanism of semantic representation and color context binding,we used electroencephalograph time frequency and coherence analyses to reveal local and long-range functional coupling in the encoding and retrieval phases of episodic *** undergraduates participated in the experiment and middle-frequency double-character Chinese words were used as stimuli in two types of study-test tasks (context recall and context recognition tests).Significant differences between item+context and item in the encoding phase were observed at the electrodes in the frontal region at 600-800 ms by time frequency *** differences were observed at 800 ms by independent component analysis:the frontal component and the coherent component of the triangle phase locking structure among the prefrontal,right parietal and left parietal-occipital *** the retrieval phase,differences between item+context and item were found on the electrodes at the central parietal and parietal-occipital regions at 400 ms by time frequency analysis,on the parietal-occipital component at 800 ms by independent component analysis and on the coherence component of the anterior right hemisphere and parietal-occipital regions at 1400 *** conclusion,the different effects of encoding and retrieval processing on 'binding' are reflected by the differing extents that brain regions engaged in cognitive *** the retrieval phase in particular,activities of the parietal-occipital region were specifically associated with 'binding',and coherence between frontal and temporal-parietal regions is a common brain activity in episodic memory.

关键词：神经机制约束力编码时间频率分析提取独立成分分析组成部分色彩范围

Bmmp influences wing morphology by regulating anterior-posterior and proximal-distal axes sdevelopment

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Insect Science 2022年第6期29卷 1569-1582页

作者： Yun-Long Zou Xin ding Li Zhang Li-Feng Xu Shu-Bo Liang Hai hu Fang-Yin Dai xiao-Ling Tong State Key Laboratory of Silkworm Genome Biology:Key Laboratory of Sericultural Biology and Genetic Breeding Ministry of Agriculture and Rural AffairsCollegeof SericultureTextileand Biomass SciencesSouthwest UniversityChongqingChina

Insect wings are subject to strong selective pressure,resulting in the evolution of remarkably diverse wing morphologies that largely determine flight ***,the genetic basis and regulatory mechanisms underlying wing size and shape development are not well *** silkworm Bombyx mori micropterous(mp)mutant exhibits shortened wing length and enlarged vein spacings,albeit without changes in total wing ***,the mp mutant comprises a valuable genetic resource for studying wing *** this study,we used molecular mapping to identify the gene responsible for the mp phenotype and designated it ***-causing mutations were identified as indels and single nucleotide polymorphisms in noncoding *** mutations resulted in decreased Bmmp messenger RNA levels and changes in transcript isoform *** null mutants were generated by clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated protein 9 and exhibited changed wing shape,similar to mp mutants,and significantly smaller total wing *** examining the expression of genes critical to wingdevelopment inwildtype and Bmmp null mutants,we found that Bmmp exerts its function by coordinately modulating anterior-posterior and proximal-distal axes *** also studied a Drosophila mp mutant and found that Bmmp is functionally conserved in *** Drosophila mp mutant strain exhibits curly wings of reduced size and a complete loss of flight *** results increase our understanding of the mechanisms underpinning insect wing development and reveal potential targets for pest control.

关键词： anterior-posterior axis Bmmp CRISPR/Cas9 proximal-distal axis silk-worm wing development

Design of Networked Control Systems with Bounded Arbitrary Time Delays

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International Journal of Automation and computing 2012年第2期9卷 182-190页

作者： xiao-Ming Tang Bao-Cang ding College of Automation Chongqing UniversityChongqing 400044PRC

This paper is mainly concerned with the model predictive control （MPC） of networked control systems （NCSs） with uncertain time delay and data packets disorder. The network-induced time delay is described as bounded and arbitrary process. For the usual state feedback controller, by considering all the possibilities of delays, an augmented state space model of the closed-loop system, which characterizes all the delay cases, is obtained. The stability conditions are given according to the Lyapunov method based on this augmented model. The stability property is inherited in MPC which explicitly considers the physical constraints. A numerical example is given to demonstrate the effectiveness of the proposed MPC.

关键词： Model predictive control (MPC) state feedback networked control systems (NCSs) robust control network-induceddelay.

Active set strategy of optimized extreme learning machine

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Chinese Science Bulletin 2014年第31期59卷 4152-4160页

作者： xiao-Jian ding Bao-Fang Chang Science and Technology on Information Systems Engineering Laboratory School of Computer and Information Engineering Henan Normal University

Extreme learning machine(ELM)has been introduced as a simple and efficient learning approach for regression and classification *** the optimization point of view,optimized ELM is equivalent to SVM,but with less constraints in the optimization formulation and random ELM *** paper introduces an active set based optimized ELM approach to solve bound constrained optimization problem in a straightforward way,which operates on a small working set of variables at each ***,the constrained problem can be eventually solved by an unconstrained algorithm,and this enables us to establish a global convergence *** approach requires less time for quadratic programming solving and provides better generalization *** addition,the proposed approach with much smaller number of nonbound support values is significantly faster than SVM with active set strategy for large training data set.

关键词：优化配方学习机有效集策略约束优化问题支持向量机 ELM 学习方法应用程序

Turning machining induced microstructural stability of a high Nb-containing TiAl alloy during high temperature exposure

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Rare Metals 2016年第1期35卷 77-84页

作者： Lu Fang Jun-Pin Lin xiao-Chan Qiu Jin-xiao Ou Xian-Fei ding State Key Laboratory for Advanced Metals and Materials University of Science and Technology Beijing School of Architecture and Engineering Heilongjiang University National Center for Materials Service Safety University of Science and Technology Beijing

Turning machining induced microstructural instability was investigated in a fully lamellar Ti-45Al-8.5Nb-（W,B,Y） alloy during high temperature *** turning machining followed by thermal exposure at900 or 1000℃ for 100,300 and 500 h,a depth-dependent gradient microstructure with random orientations was produced in the region close to the machining *** typical layers,a fine-grained（FG） layer with equiaxed grains and a coarse-grained（CG） layer with elongated grains,are formed in this region in transversal *** thickness of the two layers is up to 120 urn after thermal exposure at 1000℃ for 500 h,which is less than the depth of the hardened region（200 μm） after turning *** of the new grains in FG and CG layers are constituted of γ single phase,while short α_2 segments and few B2 particles are precipitated at the γ/γ interface or inside the γ *** and phase boundary bulging are found to be the major mechanisms responsible for lamellar degradation in FG layer and CG layer,*** residual deformation energy stored is considered to be the main driving force of this process.

关键词： High Nb-containing TiAl alloy Residualdeformation energy Microstructural instability Recrystallization Phase transformation

New hydrous phases in the Al_(2)O_(3)-SiO_(2)-H_(2)O system under the mantle transition zone conditions

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Science China Earth Sciences 2023年第4期66卷 730-737页

作者： Baoyun WANG Jin LIU Suyu FU Pu xiao Li LI Xing ding Wansheng xiao College of Material and Chemical Engineering Tongren UniversityTongren 554300China Center for High Pressure Science and Technology Advanced Research(HPSTAR) Beijing 100193China Department of Earth and Planetary Science the University of TokyoTokyo 1130033Japan State Key Laboratory of Isotope Geochemistry Guangzhou Institute of GeochemistryChinese Academy of SciencesGuangzhou 510640China Key Laboratory of Mineralogy and Metallogeny Guangzhou Institute of GeochemistryChinese Academy of SciencesGuangzhou 510640China

Hydrous minerals in the subducting slabs are potential water carriers into the deep mantle,and thus the synthesis of new hydrous phases is significant in our understanding of water circulation throughout the Earth’s *** this study,we report the two new hydrous phases,Al_(2)SiO_(6)H_(2)and Al_(5.5)Si_(4)O_(18)H_(3.5)(hereafter referred to simply as phases Psi and Phi,respectively),which are synthesized in the Al_(2)O_(3)-SiO_(2)-H_(2)O system at 15.5 GPa,1400℃and 17.5 GPa,1600℃ by using Sakura2500-ton multi-anvil *** luminescence spectra of Cr3+show the phase Psi has characteristic peaks at 687,693 and705 nm,while phase Phi has characteristic peaks at 691,696 and 708 ***-crystal X-ray diffraction (SCXRD) refinements yield a monoclinic structure of both phases (space group P2_(1)) with ideal chemical formulae of Al_(2)SiO6H2and *** determined lattice parameters for phase Psi are a=9.4168±0.0016Å,b=4.3441±0.0007Å,c=9.4360±0.002Åand β=119.726±0.005°at ambient pressure and 300 K,while the phase Phi has a=7.2549±0.0018Å,b=4.3144±0.001Å,c=8.0520±0.002Å,and β=101.740±0.009°at ambient pressure and 250 *** microprobe analyses (EPMA) show the chemical compositions of phases Psi and Phi to be Al_(1.99)Si_(0.85)O_(6)H_(2.62)and Al_(5.58)Si_(2.81)O_(18)H_(8.03),respectively,which slightly deviate from the ideal formulae inferred from SCXRD *** may result from the disorder or substitution of Al and Si by H in the crystal structures under our synthesis *** study suggests that phases Psi and Phi are the two potential water carriers at the upper part of the mantle transitions zone,providing new insights into how deep water is stored in this region.

关键词： New hydrous phases Mantle transition zone Al_(2)O_(3)-SiO_(2)-H_(2)O system Al_(2)SiO_(6)H_(2) Al_(5.5)Si_(4)O_(18)H_(3.5)