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Chinese Chemical Letters 2017年第7期28卷 1380-1384页

作者： Tian-xiao Chen Feng Ning hai-Sheng liu Ke-Feng Wu Wei li Chang-Bei Ma School of Chemistry and Biological Engineering Changsha University of Science and Technology State Key Laboratory of Medical Genetics & School of life Sciences Central South University

In this assay, a label-free fluorescent sensing platform based on triple-helix molecular switch（THMS） and G-quadruplex was developed for the detection of tetracycline. We demonstrated this approach by using THMS, which consists of a central section with a shortened 8-mer aptamer sequence with high affinity to tetracycline and flanked by two arm segments. G-rich oligonucleotide can specifically bind to thioflavin T（Th T） as a signal transduction probe（STP）. In the absence of tetracycline, THMS remains stable, the fluorescence of background is low. By the addition of target tetracycline, the aptamer-target binding results in the formation of a structured aptamer-target complex, which disassembles the THMS and releases the STP. The free STP self-assembles into G-quadruplex and specifically binds to Th T which generates a obvious fluorescence enhancement. Using the triple-helix molecular switch, the developed aptamer-based fluorescent sensing platform showed a linear relationship with the concentration of tetracycline ranging from 0.2 to 20.0 nmol/L. The detection limit of tetracycline was determined to be970.0 pmol/L. The assay avoids complicated modifications or chemical labeling, making it simple and cost-effective. So, it is expected that this aptamer-based fluorescent assay could be extensively applied in the field of food safety inspection.

关键词： Tetracycline Aptamer Label-free Triple-helix molecular switch G-quadruplex Thioflavin T

The Change of Orientation Relationships Between Austenite and α'-Martensite During Deformation in High Manganese TRIP Steel

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Acta Metallurgica Sinica(English Letters) 2015年第3期28卷 289-294页

作者： hai-Yan Yang Jing li Ping Yang School of Materials Science and Engineering University of Science and Technology Beijing

In situ observation of electron backscattering diffraction technique was used to evaluate the orientation relationships between austenite and α′-martensite（α′-M） for high manganese transformation-induced plasticity *** was noted that different from the thermal martensite,which well obeyed K-S relationship with austenite,the orientation relationship between deformation-induced α′-M with austenite changed during deformation,namely K-S and N-W relations *** clear differences existed between α′-M variants with two kinds of relationships in terms of martensitic orientation,shape and the misorientation between α′-M *** this phenomenon happened in almost all austenitic grains with different orientations investigated in this *** atom displacement mechanism through conjugate complex slips of partial dislocations in the distorted fcc lattice was applied in this article to interpret the coexistence of K-S and N-W relationships.

关键词： In situ observation Orientation relationship Electron backscattered diffraction technique(EBSD) Martensitic transformation High manganese TRIP steel

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多学科协同仿真液压挖掘机动力学研究

机械设计与制造 2024年第5期399卷 253-256,261页

作者：李贝黄正伟陈立创臧晓辉柳州职业技术学院汽车工程学院广西柳州545006

通过采集液压挖掘机作业轨迹等参数,基于Altair软件Activate和Motion模块,建立挖掘机机构-结构-液压-控制多学科协同仿真模型,以挖掘机作业轨迹为控制目标,对比分析动臂油缸、斗杆油缸、铲斗油缸仿真和试验的受力情况,结果表明:仿真与... 详细信息

通过采集液压挖掘机作业轨迹等参数,基于Altair软件Activate和Motion模块,建立挖掘机机构-结构-液压-控制多学科协同仿真模型,以挖掘机作业轨迹为控制目标,对比分析动臂油缸、斗杆油缸、铲斗油缸仿真和试验的受力情况,结果表明:仿真与试验的受力趋势相同,动臂油缸作用力平均偏差为5%、斗杆油缸作用力平均偏差为2%、铲斗油缸作用力平均偏差为2%。研究表明,基于多学科协同仿真的挖掘机模型,可以很好地模拟挖掘机作业时的油缸受力情况,该模型可减少试验次数、降低试验成本,同时为挖掘机结构优化设计提供载荷数据,具有一定的工程意义和价值。

关键词：液压挖掘机协同仿真模型作业轨迹动力学

Efficacy and safety of tacrolimus and low-dose prednisone in Chinese children with steroid-resistant nephrotic syndrome

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World Journal of Pediatrics 2020年第2期16卷 159-167页

作者： hai-Xia Chen Qia Cheng Fang li Qing-Nan He Yan Cao Zhu-Wen Yi xiao-Chuan Wu Department of Pediatrics The Second Xiangya HospitalCentral South UniversityChangsha 410011China

Background Tacrolimus,a calcineurin inhibitor,is recommended by the recent guidelines from the Kidney Disease Improving Global Outcomes Group as the first-line treatment for steroid-resistant nephrotic syndrome(SRNS),but its clinical application in China is still *** investigated the efficacy and safety of tacrolimus combined with low-dose corticosteroids in a population of Chinese children with *** In this prospective non-randomized,non-controlled study,Chinese children with SRNS who failed the previous full-dose prednisone treatment were given tacrolimus(0.1 mg/kg/day)and low-dose prednisone(0.25-0.50 mg/kg/day).We compared the overall remission rate(ORR)and adverse events in the follow-up period with this therapeutic *** A total of 76 children were enrolled into the study with an average follow-up period of 18±6 months(maximum 36 months).ORR achieved by the first,third,and sixth months was 94.7%,94.7%,and 96.0%,*** patients who attained an initial tacrolimus trough concentration(FK506C0)>6 ng/mL(60.3%)achieved *** relative risk of relapse at FK506C0limus and low-dose prednisone was safe and effective for the treatment of children with SRNS,with high remission rates observed as early as the first *** were infrequent,but tended to increase significantly with decreases in FK506C0.

关键词： Children Low-dose prednisone Steroid-resistant nephrotic syndrome Tacrolimus

Asymmetric Synthesis of N-(Diphenylphosphinyl)furfurylamine by the Enantioselective Alkylation of Furfurylimine

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Chinese Chemical Letters 2005年第9期16卷 1147-1150页

作者： hai Le ZHANG xiao Mei ZHANG Xin li Ai Qiao MI Xin CUI Yao Zhong JIANG Key Laboratory of Asymmetric Synthesis and Chirotechnology of Sichuan Province and Union Laboraotry of Asymmetric Synthesis Chengdu Institute of Organic Chemistry Chinese Academy of Sciences Chengdu 610041

Optically active N-（diphenylphosphinyl）furfurylamines 2 with good ee values were obtained by the enantioselective addition of dialkylzincs to furfuryl imine 1 in the presence of chiral aminoalcohol derivatives and o... 详细信息

关键词： Asymmetric dialkylzinc N-(diphenylphosphinyl)furfurylarnine.

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烟台葡萄酒产业创新升级路径研究

中外葡萄与葡萄酒 2024年第2期 106-111页

作者：刘世松张莉菅蓁张晓滨州医学院药学院山东烟台264003 山东烟台市葡萄与葡萄酒产业发展服务中心山东烟台264003

2013年以来,我国葡萄酒产销量连续下滑,葡萄酒产业一直处于调整与变革态势。烟台葡萄酒产业发展取得了一定的成效,在国内一度呈现引领发展的态势,但是近年来受国内外市场环境变化影响和传统经济发展方式制约,烟台葡萄酒产业面临严峻挑战... 详细信息

2013年以来,我国葡萄酒产销量连续下滑,葡萄酒产业一直处于调整与变革态势。烟台葡萄酒产业发展取得了一定的成效,在国内一度呈现引领发展的态势,但是近年来受国内外市场环境变化影响和传统经济发展方式制约,烟台葡萄酒产业面临严峻挑战,提质增效任务艰巨,亟须转型升级、创新发展。在此背景下,深入剖析烟台葡萄酒产业存在的问题,探究烟台葡萄酒全产业链综合施策路径,通过深化体制机制改革、优化提升产业布局、提高创新要素配置、打造高端沟通平台、聚焦文化引领建设等举措,实现推动葡萄酒全产业链创新升级。这对于加速推进烟台葡萄酒产业向价值链高端跃升,引导产城融合、链式发展,提高烟台葡萄酒产业发展新优势明确了方向和重点。

关键词：新旧动能转换烟台葡萄酒产业创新升级路径研究

Wuzi Yanzong Pill Plays A Neuroprotective Role in Parkinson’s Disease Mice via Regulating Unfolded Protein Response Mediated by Endoplasmic Reticulum Stress

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Chinese Journal of Integrative Medicine 2023年第1期29卷 19-27页

作者： li Yan-rong FAN Hui-jie SUN Rui-rui JIA Lu YANG li-yang ZHANG hai-fei JIN xiao-ming xiao Bao-guo MA Cun-gen Chai Zhi The Key Research Laboratory of Benefiting Qi for Acting Blood Circulation Method to Treat Multiple Sclerosis of State Administration of Traditional Chinese Medicine Shanxi University of Chinese MedicineJinzhongShanxi Province030619China Institute of Brain Science Department Neurology of First Affiliated HospitalShanxi Datong UniversityDatongShanxi Province037009China Department of Anatomy and Cell Biology Department of Neurological SurgerySpinal Cord and Brain Injury Research GroupStark Neurosciences Research InstituteIndiana University School of MedicineIndianapolisIN46202USA Institute of Neurology Huashan HospitalInstitutes of Brain Science and State Key Laboratory of Medical NeurobiologyFudan UniversityShanghai200025China

Objective:To investigate the protective effects and its possible mechanism of Wuzi Yanzong Pill(WYP) on Parkinson’s disease(PD) model ***:Thirty-six C57BL/6 male mice were randomly assigned to 3 groups including normal,PD,and PD+WYP groups,12 mice in each *** week of intraperitoneal injection of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine(MPTP) was used to establish the classical PD model in ***,mice in the PD+WYP group were administrated with 16 g/kg WYP,twice daily by *** 14 days of administration,gait test,open field test and pole test were measured to evaluate the movement *** hydroxylase(TH) neurons in substantia nigra of midbrain and binding immunoglobulin heavy chain protein(GRP78) in striatum and cortex were observed by *** levels of TH,GRP78,p-PERK,p-elF2α,ATF4,p-IRE1α,XBP1,ATF6,CHOP,ASK1,p-JNK,Caspase-12,-9 and-3 in brain were detected by Western ***:Compared with the PD group,WYP treatment ameliorated gait balance ability in PD mice(Pliorated motor symptoms and pathological lesion of PD mice,which may be related to the regulation of unfolded protein response-mediated signaling pathway and inhibiting the endoplasmic reticulum stress-mediated neuronal apoptosis pathway.

关键词： Wuzi Yanzong Pill Parkinson’s disease dopaminergic neurons endoplasmic reticulum stress apoptosis

The environment of C-and S-shaped radio galaxies

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Research in Astronomy and Astrophysics 2019年第9期19卷 55-62页

作者： Yu-Xing liu hai-Guang Xu Dong-Chao Zheng Wei-Tian li Zheng-Hao Zhu Zhi-Xian Ma xiao-li lian School of Physics and Astronomy Shanghai Jiao Tong UniversityShanghai200240China Tsung-Dao Lee Institute/IFSA Collaborative Innovation Center Shanghai Jiao Tong UniversityShanghai200240China Department of Electronic Engineering Shanghai Jiao Tong UniversityShanghai200240China

We study the environment of radio galaxies with different morphological types using the Proctor sample, which was built from the Faint Images of the Radio Sky at Twenty-Centimeters(FIRST) survey archive. Among the 15 radio galaxy types classified by Proctor, 199 C-shaped(i.e., wide-or narrow-angle tail) and 203 S-shaped(i.e., S-or Z-shaped) sources are selected in this work, which are located in the redshift range of 0.02 likely to be BCGs than the S-shaped sources. These results support the idea that C-shaped radio galaxies often reside in a richer environment than radio galaxies with other morphological types.

关键词： galaxies:statistics galaxies:structure methods:data analysis

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不同浓度EMS诱变不结球白菜的效果分析

中国蔬菜 2024年第7期 42-49页

作者：周玉彩肖栋李英何盈侯喜林南京农业大学作物遗传与种质创新利用全国重点实验室农业农村部华东地区园艺作物生物学与种质创制重点实验室园艺作物种质创新与利用教育部工程研究中心江苏南京210095 南京苏曼等离子工程研究院有限公司江苏南京211162

为创制不结球白菜新种质,发掘新基因,探索不同浓度甲基磺酸乙酯(ethyl methane sulfonate,EMS)对不结球白菜的诱变效应。采用浓度为0.4%、0.6%和0.8%的EMS溶液对4个不结球白菜品种[黑塌菜(HTC)、香青菜(XQC)、JP241、XWZ]进行种子诱变处... 详细信息

为创制不结球白菜新种质,发掘新基因,探索不同浓度甲基磺酸乙酯(ethyl methane sulfonate,EMS)对不结球白菜的诱变效应。采用浓度为0.4%、0.6%和0.8%的EMS溶液对4个不结球白菜品种[黑塌菜(HTC)、香青菜(XQC)、JP241、XWZ]进行种子诱变处理,调查种子发芽情况与田间植株农艺性状表现。结果表明:4个不结球白菜品种的种子发芽势、发芽率和相对成苗率均随着EMS浓度的升高而逐渐降低,且处理间差异显著。EMS诱变可以引起不结球白菜叶色、叶形、株型、花器官等性状发生变异。不同品种不结球白菜的EMS诱变半致死剂量有所不同,产生的变异种类和变异频率也有所不同,HTC的半致死剂量为0.8%,相对变异频率为23.83%;XQC的半致死剂量为0.8%,相对变异频率为17.61%;JP241的半致死剂量为0.6%,相对变异频率为38.45%;XWZ的半致死剂量为0.6%,相对变异频率16.69%。EMS的浓度越高,诱变的不结球白菜始花开放时间越晚,莲座叶叶片数越少,雄性不育率越高。

关键词：不结球白菜 EMS 变异频率变异类型

Development and validation of a nomogram based on perioperative risk factors for the prediction of early thrombotic complications following pediatric liver transplantation

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中华医学杂志(英文版) 2024年第16期137卷 1993-1995页

作者： You Qiang Qu Enze Zeng Kaining liu Jianrong Yang Qing Tang Hui Feng xiao Yao Jia Huang Xinru Zhang Tong Fu Binsheng li Hua Zhao Hui Zheng Jun Yi Shuhong Yang Yang Department of Hepatic Surgery and liver Transplantation Center of the Third Affiliated Hospital of Sun Yat-sen University Organ Transplantation Research Center of Guangdong Province Guangdong Province Engineering Laboratory for Transplantation Medicine Guangzhou Guangdong China Guangdong Key Laboratory of liver Disease Research Key Laboratory of Liver Disease Biotherapy and Translational Medicine of Guangdong Higher Education Institutes The Third Affiliated Hospital of Sun Yat-sen University Guangzhou Guangdong China Department of Ultrasound The Third Affiliated Hospital of Sun Yat-Sen University Guangzhou Guangdong China Surgical ICU of the Third Affiliated Hospital of Sun Yat-sen University Guangzhou Guangdong China