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Effects of extracellular vesicles from mesenchymal stem cells on oxygen-glucose deprivation/reperfusioninduced neuronal injury

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World Journal of Emergency Medicine 2021年第1期12卷 61-67页

作者： Shuang-shuang Gu Xiu-wen Kang Jun Wang xiao-fang guo Hao Sun Lei Jiang Jin-song Zhang Department of Emergency the First Affi liated Hospital of Nanjing Medical UniversityNanjing 210029China Key Laboratory of Modern Toxicology Ministry of EducationDepartment of ToxicologySchool of Public HealthNanjing Medical UniversityNanjing 211166China

BACKGROUND: Small extracellular vesicles (sEVs) from bone marrow mesenchymal stemcells (BMSCs) have shown therapeutic potential for cerebral ischemic diseases. However, themechanisms by which BMSC-derived sEVs (BMSC-sEVs) protect neurons against cerebral ischemia/reperfusion (I/R) injury remain unclear. In this study, we explored the neuroprotective effects ofBMSC-sEVs in the primary culture of rat cortical neurons exposed to oxygen-glucose deprivation andreperfusion (OGD/R) ***: The primary cortical neuron OGD/R model was established to simulate the processof cerebral I/R in vitro. Based on this model, we examined whether the mechanism through whichBMSC-sEVs could rescue OGD/R-induced neuronal ***: BMSC-sEVs (20 μg/mL, 40 μg/mL) significantly decreased the reactive oxygenspecies (ROS) productions, and increased the activities of superoxide dismutase (SOD) and glutathioneperoxidase (GPx). Additionally, BMSC-sEVs prevented OGD/R-induced neuronal apoptosis in vivo, asindicated by increased cell viability, reduced lactate dehydrogenase (LDH) leakage, decreased terminaldeoxynucleotidyl transferase (TdT)-mediated dUTP nick end labeling (TUNEL) staining-positivecells, down-regulated cleaved caspase-3, and up-regulated Bcl-2/Bax ratio. Furthermore, Westernblot and flow cytometry analysis indicated that BMSC-sEV treatment decreased the expression ofphosphorylated calcium/calmodulin-dependent kinase II (p-CaMK II)/CaMK II, suppressed the increaseof intracellular calcium concentration ([Ca2+]i) caused by OGD/R in ***: These results demonstrate that BMSC-sEVs have signifi cant neuroprotectiveeff ects against OGD/R-induced cell injury by suppressing oxidative stress and apoptosis, and Ca2+/CaMK II signaling pathways may be involved in this process.

关键词： Oxygen-glucose deprivation and reperfusion Cortical neurons Oxidative stress Small extracellular vesicles

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Feasibility of MRI Radiomics for Predicting KRAS Mutation in Rectal Cancer

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Current Medical Science 2020年第6期40卷 1156-1160页

作者： xiao-fang guo Wen-qian YANG Qian YANG Zi-long YUAN Yu-lin LIU xiao-hui NIU Hai-bo XU Department of Radiology Zhongnan Hospital of Wuhan UniversityWuhan 430071China Department of Radiology Hubei Cancer HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430079China College of Informatics Huazhong Agricultural UniversityWuhan 430070China

The mutation status of KRAS is a significant biomarker in the prognosis of rectal *** study investigated the feasibility of MRI-based radiomics in predicting the mutation status of KRAS with a composite index which could be an important criterion for KRAS mutation in clinical *** this retrospective study,a total of 127 patients with rectal cancer were *** 3D Slicer was used to extract the radiomics features from the MRI images,and sparse support vector machine(SVM)with linear kernel was applied for feature *** radiomics classifier for predicting the KRAS status was then constructed by Linear Discriminant Analysis(LDA)and its performance was *** composite index was determined with LDA *** of 127 rectal cancer subjects,there were 44 KRAS mutation cases and 83 wild cases.A total of 104 radiomics features were extracted,54 features were filtered by linear SVM with L1-norm regularization and 6 features that had no significant correlations within them were finally *** radiomics classifier constructed using the 6 features featured an AUC value of 0.669(specificity:0.506;sensitivity:0.773)with ***,the composite index(Radscore)had statistically significant difference between the KRAS mutation and wild *** is suggested that the MRI-based radiomics has the potential in predicting the KRAS status in patients with rectal cancer,which may enhance the diagnostic value of MRI in rectal cancer.

关键词： rectal cancer KRAS mutation MRI radiomics

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Protective effect of mesenchymal stem cell-derived exosomal treatment of hippocampal neurons against oxygen-glucose deprivation/reperfusion-induced injury

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World Journal of Emergency Medicine 2022年第1期13卷 46-53页

作者： xiao-fang guo Shuang-shuang Gu Jun Wang Hao Sun Yu-juan Zhang Peng-fei Yu Jin-song Zhang Lei Jiang Department of Emergency the First Affi liated Hospital of Nanjing Medical UniversityNanjing 210029China Key Laboratory of Modern Toxicology Ministry of EducationDepartment of ToxicologySchool of Public HealthNanjing Medical UniversityNanjing 211166China Key Laboratory of Modern Toxicology Ministry of EducationDepartment of Environmental GenomicsSchool of Public HealthNanjing Medical UniversityNanjing 211166China Center for Drug Safety Evaluation and Research Shanghai Institute of Materia MedicaShanghai 201203China

BACKGROUND:Individuals who survive a cardiac arrest often sustain cognitive impairments due to ischemia-reperfusion *** stem cell(MSC)transplantation is used to reduce tissue damage,but exosomes are more stable and highly conserved than *** study was conducted to investigate the therapeutic effects of MSC-derived exosomes(MSC-Exo)on cerebral ischemia-reperfusion injury in an in vitro model of oxygen-glucose deprivation/reperfusion(OGD/R),and to explore the underlying ***:Primary hippocampal neurons obtained from 18-day Sprague-Dawley rat embryos were subjected to OGD/R treatment,with or without MSC-Exo *** integration,cell viability,mitochondrial membrane potential,and generation of reactive oxygen species(ROS)were *** deoxynucleotidyl transferase-mediated 2’-deoxyuridine 5’-triphosphate nickend labeling(TUNEL)staining was performed to detect neuronal ***,mitochondrial function-associated gene expression,Nrf2 translocation,and expression of downstream antioxidant proteins were ***:MSC-Exo attenuated OGD/R-induced neuronal apoptosis and decreased ROS generation(P<0.05).The exosomes reduced OGD/R-induced Nrf2 translocation into the nucleus(2.14±0.65 vs.5.48±1.09,P<0.01)and increased the intracellular expression of antioxidative proteins,including superoxide dismutase and glutathione peroxidase(17.18±0.97 vs.14.40±0.62,and 20.65±2.23 vs.16.44±2.05,respectively;P<0.05 for both).OGD/R significantly impaired the mitochondrial membrane potential and modulated the expression of mitochondrial functionassociated genes,such as PINK,DJ1,LRRK2,Mfn-1,Mfn-2,and *** abovementioned changes were partially reversed by exosomal treatment of the hippocampal ***:MSC-Exo treatment can alleviate OGD/R-induced oxidative stress and dysregulation of mitochondrial function-associated genes in hippocampal ***,MSCExo might be a potential therapeutic strategy to preven

关键词： Mesenchymal stem cells Exosomes Oxygen-glucose deprivation/reperfusion Reactive oxygen species Mitochondria

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Constructing a nomogram to predict overall survival of colon cancer based on computed tomography characteristics and clinicopathological factors

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World Journal of Gastrointestinal Oncology 2024年第10期16卷 4104-4114页

作者： Zhe-Xing Hu Yin Li Xuan Yang Yu-Xia Li Yao-Yao He xiao-Hui Niu Ting-Ting Nie xiao-fang guo Zi-Long Yuan Department of Radiology Hubei Cancer HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430079Hubei ProvinceChina Department of Radiology Wuhan Children’s HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430014Hubei ProvinceChina College of Informatics Huazhong Agriculture UniversityWuhan 430070Hubei ProvinceChina

BACKGROUND The colon cancer prognosis is influenced by multiple factors,including clinical,pathological,and non-biological ***,only a few studies have focused on computed tomography(CT)imaging ***,this study aims to predict the prognosis of patients with colon cancer by combining CT imaging features with clinical and pathological characteristics,and establishes a nomogram to provide critical guidance for the individualized *** To establish and validate a nomogram to predict the overall survival(OS)of patients with colon *** A retrospective analysis was conducted on the survival data of 249 patients with colon cancer confirmed by surgical pathology between January 2017 and December *** patients were randomly divided into training and testing groups at a 1:1 *** and multivariate logistic regression analyses were performed to identify the independent risk factors associated with OS,and a nomogram model was constructed for the training *** curves were calculated using the Kaplan–Meier *** concordance index(C-index)and calibration curve were used to evaluate the nomogram model in the training and testing *** Multivariate logistic regression analysis revealed that lymph node metastasis on CT,perineural invasion,and tumor classification were independent prognostic factors.A nomogram incorporating these variables was constructed,and the C-index of the training and testing groups was 0.804 and 0.692,*** calibration curves demonstrated good consistency between the actual values and predicted probabilities of *** A nomogram combining CT imaging characteristics and clinicopathological factors exhibited good discrimination and *** can aid clinicians in risk stratification and postoperative monitoring and provide important guidance for the individualized treatment of patients with colon cancer.

关键词： Colon cancer Nomogram Prognosis Overall survival Computed tomography Clinicopathology

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Developmental expression of three prmt genes in Xenopus

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Zoological Research 2019年第2期40卷 102-107页

作者： Cheng-Dong Wang xiao-fang guo Thomas Chi Bun Wong Hui Wang Xu-Feng Qi Dong-Qing Cai Yi Deng Hui Zhao Key Laboratory for Regenerative Medicine Ministry of EducationSchool of Biomedical Sciences Faculty of Medicine The Chinese University of Hong Kong Key Laboratory for Regenerative Medicine of Ministry of Medicine of Ministry of Education Jinan University Department of Developmental & Regenerative Biology College of Life Science and Technology Jinan University Guangdong Provincial Key Laboratory of Cell Microenvironment Department of Biology South University of Science and Technology of China Kunming Institute of Zoology Chinese Academy of Sciences-The Chinese University of Hong Kong Joint Laboratory of Bioresources and Molecular Research of Common Diseases

DEAR EDITOR Protein arginine methyltransferases (PRMTs)are involved in many cellular processes via the arginine methylation of histone or non-histone *** examined the expression patterns of prmt4,prmt7,and prmt9 during embryogenesis in Xenopus using whole-mount in situ hybridization and quantitative reverse transcription polymerase chain reaction (RT-PCR).Xenopus prmt4 and prmt7 were expressed in the neural crest,brain,and spinal cord,and also detected in the eye,branchial arches,and heart at the tailbud *** print9 signals were not detected in Xenopus embryos until the late tailbud stage when weak expression was observed in the branchial *** RT-PCR indicated that the expression of prmt4 and prmt7 was up-regulated during the neurula stage,whereas prmt9 maintained its low expression until the late tailbud stage,consistent with the whole-mount in situ hybridization ***,the developmental expression patterns of these three print genes in Xenopus embryos provide a basis for further functional study of such genes.

关键词： Developmental expression prmt genes Xenopus

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Adeno-associated virus vectors simultaneously encoding VEGF and angiopoietin-1 enhances neovascularization in ischemic rabbit hindlimbs

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Acta Pharmacologica Sinica 2007年第4期28卷 493-502页

作者： Feng CHEN Zui TAN Department of Vascular Surgery,Zhongnan Hospital,Wuhan UniversityChang-yuan DONG xiao CHEN Shu-fang guo State Key Laboratory of Virology,Medical College,Wuhan University,Wuhan 430071,China Department of Vascular Surgery Zhongnan Hospital Wuhan University Wuhan China State Key Laboratory of Virology Medical College Wuhan University Wuhan China

Aim:Angiopoietin-1 (Ang1) and vascular endothelial growth factor A (VEGF)play important roles in vascular formation and maturation,suggesting a combina-tion of these 2 would be a promising therapy for ischemic *** we con-structed an adeno-associated virus (AAV) vector,simultaneously encoding hu-man VEGF165and Ang 1 (AAV-VEGF/Ang 1),and investigated its therapeutic effectin a rabbit ischemic hind-limb ***:Four experimental groups wereused to prepare the rabbit ischemic hind-limb model following AAV vectors intra-muscular administration as follows:PBS (phosphate buffered solution),AAV-VEGF,AAV-Ang1,AAV-VEGF/***:Eight weeks after administration,humanVEGF165and Ang1 were detected by RT-PCR,Western blotting and histochemicalstaining methods in AAV-VEGF/Ang1 transduced *** AAV-VEGF/Ang1 showed a significantly increased blood-flow recovery in ischemic hind-limbs compared with the other *** staining for alkaline phos-phatase showed that capillary density of group AAV-VEGF/Ang1 or AAV-VEGFwas significantly higher than that of group PBS or *** for smooth muscle α-actin (α-SMA) revealed that group AAV-VEGF/Ang 1 had the highest density of α-SMA-positive vessels compared withthe other *** leakage,one of the major adverse effects induced byVEGF,was very severe in group AAV-VEGF,but the permeability was obviouslyreduced when VEGF was co-expressed with Ang1 in group AAV-VEGF/***:AAV vectors can simultaneously encode 2 proteins which can beefficiently and stably co-expressed in transduced ***-mediated VEGFand Ang1 gene transfer enhances neovascularization,prevents capillary leakage,and improves blood flow in a rabbit hind-limb ischemic *** findingssuggest that intramuscular administration of AAV-VEGF/Ang 1 may be useful inthe treatment of ischemic diseases.

关键词： adeno-associated virus vascular endothelial growth factor A angiopoietin-l neovascularization ischemia

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创新驱动视域下的研究型大学创新能力提升机制研究

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科学管理研究 2019年第3期37卷 20-23页

作者：肖国芳上海交通大学改革与发展研究室

研究型大学是创新驱动发展的核心支撑力量,具有自身独特优势的同时也存在不少瓶颈问题。有针对性地提出对于提升我国研究型大学创新能力的思考:坚持科技创新和体制机制创新'双轮驱动',加强研究型大学的创新能力开放合作与融合;打破研究... 详细信息

研究型大学是创新驱动发展的核心支撑力量,具有自身独特优势的同时也存在不少瓶颈问题。有针对性地提出对于提升我国研究型大学创新能力的思考:坚持科技创新和体制机制创新'双轮驱动',加强研究型大学的创新能力开放合作与融合;打破研究型大学内部的学科壁垒,大力推进学科交叉研究;突出质量和贡献导向,完善研究型大学创新能力评估体系。

关键词：创新驱动研究型大学创新能力开放合作区域创新集群

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博士生创新能力培养的瓶颈问题与制度构建

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科学管理研究 2019年第5期37卷 127-132页

作者：肖国芳彭术连上海交通大学改革与发展研究室上海200240 上海交通大学设计学院上海200240

培养创新人才,就是培育国家创新能力。博士生创新能力具有建构性、习得性,时代社会性和群体文化性,相对稳定性。从个体因素、导师指导、保障体系等方面分析了博士生创新能力培养存在的瓶颈问题,并提出制度构建:聚焦创新能力,推进博士生... 详细信息

培养创新人才,就是培育国家创新能力。博士生创新能力具有建构性、习得性,时代社会性和群体文化性,相对稳定性。从个体因素、导师指导、保障体系等方面分析了博士生创新能力培养存在的瓶颈问题,并提出制度构建:聚焦创新能力,推进博士生招生与培养制度改革;加强创新文化建设和创新习性培育,强化创新导向和压力传导;提升导师水平和指导能力,规范和强化导师的督导行为;构建全方位的评估体系,建立预警机制、完善过程管理。

关键词：博士生创新能力培养创新习性制度构建

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科技自立自强背景下研究型大学有组织科研的逻辑、困境与进路

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科学管理研究 2024年第3期42卷 26-34页

作者：彭术连肖国芳上海交通大学安泰经济与管理学院上海200240 上海交通大学设计学院上海200240 上海交通大学教育学院上海200240

高等教育强国和科技自立自强作为近年的热点政策话语,体现了教育、科技和人才三位一体在推动中国式现代化进程中的重要作用。在科技自立自强背景下,我国研究型大学科研面临着逻辑转向,在科研使命上从经济使命转向战略使命,在科研动力从... 详细信息

高等教育强国和科技自立自强作为近年的热点政策话语,体现了教育、科技和人才三位一体在推动中国式现代化进程中的重要作用。在科技自立自强背景下,我国研究型大学科研面临着逻辑转向,在科研使命上从经济使命转向战略使命,在科研动力从自由兴趣转向国家意志,在科研内容从单一领域转向多维立体,在科研视角上从学术场域转向社会场域。面向科技自立自强研究型大学有组织科研存在多元困境,应强化国家使命担当,不断优化研究型大学有组织科研顶层设计;改革高校内部治理,促进有组织科研的整合式创新;充分发挥战略科技人才引领作用;坚持全球开放合作,打造有组织科研的互惠共享国际合作网络;重塑尊重创新质量和贡献的学术评价制度。

关键词：中国式现代化科技自立自强研究型大学有组织科研

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新制度主义视角下高校职务科技成果转化的困境与路向研究

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科学管理研究 2020年第5期38卷 65-70,82页

作者：肖国芳彭术连上海交通大学改革与发展研究室上海200240 上海交通大学设计学院上海200240

高校职务科技成果转化已成为困扰国家系统创新链的关键问题之一,新制度主义理论为这一问题的研究提供了新的分析框架和视角。制度资源短缺、主体间的利益博弈与文化认同缺失,是面临的主要困境,也是破解问题的重要入口。推进高校职务科... 详细信息

高校职务科技成果转化已成为困扰国家系统创新链的关键问题之一,新制度主义理论为这一问题的研究提供了新的分析框架和视角。制度资源短缺、主体间的利益博弈与文化认同缺失,是面临的主要困境,也是破解问题的重要入口。推进高校职务科技成果转化,需要政府和高校完善制度建构,建立在以最大化个人利益为特征的“理性人”假设基础之上加强制度创新。从政府而言,需要进一步推动组织变革,落实高校法人自主权;改革高校职务科技成果作为国有无形资产的管理模式;从高校而言,应基于“理性人”建立激励兼容的科技成果转化制度,建立专业化、市场化、统一化的科技成果转化体系。

关键词：新制度主义科技成果转化路向选择理性人

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