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The endo-beta mannase MAN7 contributes to cadmium tolerance by modulating root cell wall binding capacity in Arabidopsis thaliana

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Journal of Integrative Plant Biology 2023年第7期65卷 1670-1686页

作者： Qi Wu Yu Ting meng Zhi Hang Feng Ren Fang Shen xiao Fang Zhu State Key Laboratory of Soil and Sustainable Agriculture Institute of Soil ScienceChinese Academy of SciencesNanjing 210008China University of Chinese Academy of Sciences Beijing 100049China College of Environmental and Resource Sciences Zhejiang UniversityHangzhou 310058China

The heavy metal cadmium(Cd) is detrimental to crop growth and threatens human health through the food chain. To cope with Cd toxicity,plants employ multiple strategies to decrease Cd uptake and its root-to-shoot translocation. However, genes that participate in the Cd-induced transcriptional regulatory network, including those encoding transcription factors, remain largely unidentified. In this study, we demonstrate that ENDO-BETA-MANNASE 7(MAN7) is necessary for the response of Arabidopsis thaliana to toxic Cd levels. We show that MAN7 is responsible for mannase activity and modulates mannose content in the cell wall, which plays a role in Cd compartmentalization in the cell wall under Cd toxicity conditions. Additionally,the repression of root growth by Cd was partially reversed via exogenous application of mannose,suggesting that MAN7-mediated cell wall Cd redistribution depends on the mannose ***, we identified a basic leucine zipper(bZIP) transcription factor, bZIP44, that acts upstream of MAN7 in response to Cd *** dual-luciferase assays indicated that bZIP44 directly binds to the MAN7 promoter region and activates its transcription. Loss of bZIP44 function was associated with greater sensitivity to Cd treatment and higher accumulation of the heavy metal in roots and ***, MAN7 overexpression relieved the inhibition of root elongation seen in the bzip44mutant under Cd toxicity conditions. This study thus reveals a pathway showing that MAN7-associated Cd tolerance in Arabidopsis is controlled by bZIP44 upon Cd exposure.

关键词： Arabidopsis thaliana bZIP44 cadmium cell wall MAN7 mannose

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Defect band formation in high pressure die casting AE44 magnesium alloy

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China Foundry 2022年第3期19卷 201-210页

作者： Ying-ying Hou meng-wu Wu Feng Huang xiao-bo Li Shou-mei Xiong Hubei Key Laboratory of Advanced Technology for Automotive Components Wuhan University of TechnologyWuhan 430070China School of Materials Science and Engineering Tsinghua UniversityBeijing 100084China

The characteristics of defect bands in the microstructure of high pressure die casting(HPDC)AE44 magnesium alloy were *** attention was paid to the effects of process parameters during the HPDC process and casting structure on the distribution of defect *** show that the defect bands are solute segregation bands with the enrichment of Al,Ce and La elements,which are basically in the form of Al_(11)RE_(3) *** is no obvious aggregation of porosities in the defect *** width of the inner defect band is 4-8 times larger than that of the outer *** variation trends of the distribution of the inner and outer defect bands are not consistent under different process parameters and at different locations of *** is due to the discrepancy between the formation mechanisms of double defect *** filling and solidification behavior of the melt near the chilling layer is very complicated,which finally leads to a fluctuation of the width and location of the outer defect *** affecting the content and aggregation degree of externally solidified crystals(ESCs)in the cross section of die castings,the process parameters and casting structure have a great influence on the distribution of the inner defect band.

关键词： high pressure die casting magnesium alloy AE44 defect band microstructure

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基于“滞-郁-虚”动态演变探讨慢性应激诱导抑郁的病机特点

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中华中医药杂志 2024年第3期39卷 1563-1566页

作者：佘楷杰孟丹华孟晓莹潘明敏梁文青岳广欣中国中医科学院中医基础理论研究所北京100700 暨南大学中医学院广州510632 河南中医药大学基础医学院郑州450046

文章回溯了滞、郁、虚的概念发展源流及演变,从字形、字义角度解释滞、郁、虚的内涵,对精神疾病中定义模糊的滞、郁病机进行深层涵义的挖掘和对比研究。滞代指气机不畅、病理产物蓄积、脏腑功能失调的病机特征;郁在囊括滞的基础上强调... 详细信息

文章回溯了滞、郁、虚的概念发展源流及演变,从字形、字义角度解释滞、郁、虚的内涵,对精神疾病中定义模糊的滞、郁病机进行深层涵义的挖掘和对比研究。滞代指气机不畅、病理产物蓄积、脏腑功能失调的病机特征;郁在囊括滞的基础上强调疾病层面的忧郁病症表现,滞为郁的内在病理基础,郁为滞的外在行为表象。虚为不足,为抑郁中后阶段的主要特征。在此基础上阐述慢性应激诱导抑郁的“滞-郁-虚”病机演变特点:滞是病理基础;郁是发病机转,是抑郁行为发生的标志;虚是结局指标,是疾病加重、病程延长的病机枢纽。针对“滞-郁-虚”不同阶段,治疗当采取行气、解郁、补虚等治则,并根据具体病机、病机兼杂、病机程度而随证加减治疗。

关键词：滞-郁-虚慢性应激抑郁病机动态演变

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甜瓜CmEPF基因家族的鉴定及表达分析

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生物技术通报 2024年第4期40卷 130-138页

作者：陈春林李白雪李金玲杜清洁李猛肖怀娟河南农业大学园艺学院郑州450046

【目的】表皮模式因子(epidermal patterning factor,EPF)是一类植物特有的分泌蛋白,在植物生长发育特别是气孔形态建成过程中发挥着重要作用,旨为揭示甜瓜EPF基因的特性和功能。【方法】对甜瓜CmEPF基因家族进行基因组鉴定,利用生物信... 详细信息

【目的】表皮模式因子(epidermal patterning factor,EPF)是一类植物特有的分泌蛋白,在植物生长发育特别是气孔形态建成过程中发挥着重要作用,旨为揭示甜瓜EPF基因的特性和功能。【方法】对甜瓜CmEPF基因家族进行基因组鉴定,利用生物信息学手段对其基因结构、系统进化以及组织表达特性进行分析。【结果】甜瓜基因组存在11个可分为3个亚家族的CmEPF成员,它们的基因结构相对保守,含有1-3个内含子,且大都分布于甜瓜的叶绿体上;基于系统进化分析将其分为3个亚家族。不同器官RT-qPCR分析发现,CmEPFs基因在甜瓜器官中广泛表达,但不同家族成员在不同器官中的表达模式存在差异,其在第二片叶表达量较高,且气孔密度、光合速率和蒸腾速率最高,其表达量与光合参数和气孔密度显著相关。【结论】CmEPFs基因通过影响甜瓜的气孔密度,影响其光合作用,从而调控甜瓜叶片气孔对外界环境的响应。

关键词：甜瓜表皮模式因子拟南芥气孔叶绿体基因表达分析

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A Study on the Relationship Between Land Finance and Housing Price in Urbanization Process: An Empirical Analysis of 182 Cities in China Based on Threshold Panel Models

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Journal of Systems Science and Information 2021年第1期9卷 74-94页

作者： Meiting HU Jichang dong Lijun YIN Chun meng Xiuting LI School of Econormics and Management University of Chinese Acaderny of SciencesBeijing 100190China School of Econormics and Management Universily of Chinese Acadermy of SciencesBeijing 100190China School of Economics and Management University of Chinese Academy of SciencesBeijing 100190China Development Research Centre of the State Council Beijing 100010China School of Econormics and Management University of Chinese Academy of SciencesBeijing 100190China

Scholars have a variety of theoretical explanations for housing price growth. However, few scholars have studied the internal influence mechanism among urbanization, land finance, and housing price. Based on the data of 182 prefecture-level cities from 2009 to 2016, this paper studies the influence of land finance on housing price under different urbanization rate levels. The study finds that with the increase of urbanization rate, the effect of land finance on housing price presents a "U" ***, an increase in land finance by 1% results in a corresponding increase in average housing price by 0.18%, with relatively low urbanization rate, 0.06% with medium level of urbanization rate,and 0.38% with high level of urbanization rate.

关键词： urbanization land finance housing price threshold panel model

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Chinese Physics B 2021年第1期30卷 1-13页

作者： Yuan-Da Liao xiao-Yan Xu Zi-Yang meng Jian Kang Beijing National Laboratory for Condensed Matter Physics and Institute of Physics Chinese Academy of SciencesBeijing 100190China School of Physical Sciences University of Chinese Academy of SciencesBeijing 100190China Department of Physics University of California at San DiegoLa JollaCalifornia 92093USA Department of Physics and HKU-UCAS Joint Institute of Theoretical and Computational Physics The University of Hong KongPokfulam RoadHong Kong SARChina Songshan Lake Materials Laboratory Dongguan 523808China School of Physical Science and Technology&Institute for Advanced Study Soochow UniversitySuzhou 215006China

We review analytical and numerical studies of correlated insulating states in twisted bilayer graphene, focusing on real-space lattice models constructions and their unbiased quantum many-body solutions. We show that by constructing localized Wannier states for the narrow bands, the projected Coulomb interactions can be approximated by interactions of cluster charges with assisted nearest neighbor hopping terms. With the interaction part only, the Hamiltonian is SU(4)symmetric considering both spin and valley degrees of freedom. In the strong coupling limit where the kinetic terms are neglected, the ground states are found to be in the SU(4) manifold with degeneracy. The kinetic terms, treated as perturbation, break this large SU(4) symmetry and propel the appearance of intervalley coherent state, quantum topological insulators, and other symmetry-breaking insulating states. We first present the theoretical analysis of moiré lattice model construction and then show how to solve the model with large-scale quantum Monte Carlo simulations in an unbiased manner. We further provide potential directions such that from the real-space model construction and its quantum many-body solutions how the perplexing yet exciting experimental discoveries in the correlation physics of twisted bilayer graphene can be gradually understood. This review will be helpful for the readers to grasp the fast growing field of the model study of twisted bilayer graphene.

关键词： twisted bilayer graphene correlated insulator quantum Monte Carlo simulation

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“双元制”下职业教育学分银行的现实困境、发展方向与改进路径

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教育理论与实践 2024年第6期44卷 13-17页

作者：赵玉青吕梦南阳师范学院教育科学学院南阳农业职业学院河南南阳473000

职业教育学分银行建设是强化职业教育类型属性、推动技能型社会建设、打造国家资历框架的综合性项目,也是推动职普融通、产教融合、科教融汇的系统性工程。在职业教育与学术教育“双元制”育人范式下,职业教育学分银行还存在框架制度不... 详细信息

职业教育学分银行建设是强化职业教育类型属性、推动技能型社会建设、打造国家资历框架的综合性项目,也是推动职普融通、产教融合、科教融汇的系统性工程。在职业教育与学术教育“双元制”育人范式下,职业教育学分银行还存在框架制度不完善、功能结构不健全、管理运行有滞碍、评估评价尚待改进等制约性因素,外部应用还存在机制、跨界、管理的时度效等制度性障碍。当前,必须明确学分银行未来建设发展的方向,致力打造成为教育与技术协同互动的桥梁纽带、职业教育高质量发展的统筹抓手、职业教育系统化改革的重要内容。因此,要以“双元制”范式为指引,建立学分银行的组织框架,健全学分银行的功能属性,创新学分银行管理模式,规范学分银行评价体系,真正推动职业教育学分银行赋能、提质、增效。

关键词：职业教育学分银行 “双元制” 职普融通

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Molecular characterization of three Hsp90 from Pieris and expression patterns in response to cold and thermal stress in summer and winter diapause of Pieris melete

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Insect Science 2018年第2期25卷 273-283页

作者： Yue-Kun Wu Chao Zou Dao-meng Fu Wan-Na Zhang Hai-Jun xiao Institute of Entomology Jiangxi Agricultural University Nanchang China Institute of Plant Protection Chinese Academy of Agricultural Sciences Beijing China

Heat shock proteins （Hsps） have been linked to stresses and winter diapause in insects, but whether they are components of summer diapause is still unknown. In this study, complementary DNAs of lisp90 from Pieris melete, Pieris rapae and Pieris canidia named PmHsp90, PrHsp90 and PcHsp90, respectively, were cloned and sequenced. The deduced amino acid sequence consisted of 718 amino acid residues with a putative molecular mass of 82.6, 82.6 and 82.7 kDa, respectively. The amino acid sequences contained all of the five conserved signature motifs in the Hsp90 family and a bHLH protein folding activity region. The differential expression pattern of PmHsp90 in response to summer diapause and winter diapause, which are related to heat/cold stress, was investigated. Cold stress induced Hsp90 up-regulation in summer and winter diapause pupae, but not in non-diapause individuals. Heat shock up-regulated PmHsp90 gradually with an increase in temperature in summer diapause, and PmHsp90 was rapidly up-regulated in winter diapause. After 30 min heat shock at 39℃, substantial up-regulation of PmHsp90 transcript levels were observed both in summer and winter diapause. However, in non-diapause a relatively stable expression was found under different durations of 39℃ heat shock. Compared to the optimal treatment of 18℃ for diapause development, a high temperature acclimation of 31 ℃ induced PmHsp90 up-regulation in summer diapause, whereas a low temperature acclimation of 4℃ induced up-regulation in winter diapause. The current results indicate that Hsp90 may play an important role in response to heat/cold stress both in summer and winter diapause.

关键词： Hsp90 Pieris melete Pieris rapae Pieris canidia summer and winterdiapause cold and heat shock

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基于Levy辨识法的IPMC等效电路迟滞建模与验证

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国外电子测量技术 2024年第4期43卷 92-98页

作者：李森安坤郭立山李宁范东孟江中北大学电气与控制工程学院太原030051 中北大学机械工程学院太原030051

离子聚合物-金属复合材料(ionic polymer-metal composite, IPMC)是一种新型柔性智能材料,但其致动机理尚不明确,位移跟踪困难。针对IPMC悬臂梁器件在低频交流驱动电压下具有明显的迟滞非线性、建模困难的特点,测试了IPMC悬臂梁在低频... 详细信息

离子聚合物-金属复合材料(ionic polymer-metal composite, IPMC)是一种新型柔性智能材料,但其致动机理尚不明确,位移跟踪困难。针对IPMC悬臂梁器件在低频交流驱动电压下具有明显的迟滞非线性、建模困难的特点,测试了IPMC悬臂梁在低频交流电压下的致动特性,分析了IPMC在0.1~10 Hz、2~5.5 V的增益与相位,并使用Levy辨识法建立了驱动电压到致动位移的三阶传递函数模型。基于已建立的传递函数模型,提出了IPMC的非线性等效电路与一阶传递函数的分段建模方法,改进了IPMC的等效电路模型无法预测致动位移的缺点。通过实验对比,结果表明,在IPMC悬臂梁器件在0.1~5 Hz、2~5.5 V的低频正弦驱动电压下,可使用等效电路加一阶传递函数代替IPMC复杂的动力学过程,整体误差低于0.3 mm,平均相对误差2.89%,满足应用条件。可以用作IPMC的低频交流用途,提高了IPMC位移跟踪精度。

关键词： IPMC迟滞特性 Levy辨识传递函数等效电路建模

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Temperature-insensitive sensor for glucose brix measurement based on compact spindle-shaped structure with two-mode fiber

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Optoelectronics Letters 2022年第12期18卷 737-742页

作者： dong Miaoyun TONG Zhengrong ZHANG Weihua LI Peng WU meng TIAN Xue Engineering Research Center of Optoelectronic Devices and Communication Technology Ministry of EducationTianjin Key Laboratory of Film Electronic and Communication DevicesSchool of Integrated Circuit Science and EngineeringTianjin University of TechnologyTianjin300384China

A temperature-insensitive sensor for glucose brix measurement based on compact spindle-shaped structure with two-mode fiber(TMF) is proposed. Due to the bending of optical fiber caused by flame baking, some of the light energy transmitted in the core leaks into the cladding area as an evanescent wave, which excites the higher-order cladding mode of the sensor. The experimental results show that when the length of TMF is 3 cm and the bending diameter is 4 mm, the maximum glucose brix sensitivity of the sensor is 0.368 nm/% from 0 to 21%. The sensor is insensitive to temperature from 10 °C to 50 °C, which can avoid the problem of temperature cross-sensitivity. A compact spindle-shaped sensor is a potential effective sensor with a simple structure, easy fabrication and low cost. The sensor can be used to detect glucose content in areas such as crops quality assessment and the research of pharmacy and bioengineering.

关键词： shaped fiber mode

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