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Journal of Ocean University of China 2022年第4期21卷 926-934页

作者： liu xiao liu Yong Shandong Provincial Key Laboratory of Ocean Engineering Ocean University of ChinaQingdao 266100China

The characteristics of irregular wave reflection by a perforated caisson breakwater under wave overtopping conditions were investigated using physical model *** effects of various parameters,especially the mean overtopping discharge that was mainly determined by the relative crest freeboard,on the reflection coefficient of perforated caisson breakwater were analyzed using experimental *** results showed that the wave overtopping occurrence had no appreciable effect on the reflection coefficient when the mean overtopping discharge was less than 0.2 m^(3)(m s)^(−1).Under the wave overtopping condition,the reflection coefficient of the perforated caisson breakwater in this study was reduced by 20%-50% compared with that of the non-perforated caisson breakwater.A predictive formula of the reflection coefficient for perforated caisson breakwaters in terms of relative water depth,relative chamber width,and porosity of the caisson front wall,was developed for practical engineering design.

关键词： perforated caisson model test reflection coefficient wave overtopping predictive formula

Proliferation Inhibitory Activity of Quinones from Blaps rynchopetera Defense Secretion on Colorectal Tumor Cells

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Chinese Journal of Integrative Medicine 2023年第8期29卷 683-690页

作者： QIAN xiao-li Meng di liu Heng liu Chao-he ZHOU Ping YANG Yin-he WANG Jia-peng xiao Huai diNG Zhong-tao Yunnan Provincial Key Laboratory of Entomological Biopharmaceutical R&D DaliYunnan Province(671003)China College of Pharmacy Dali UniversityDaliYunnan Province(671003)China Functional Molecules Analysis and Biotransformation Key Laboratory of Universities in Yunnan Province Kunming(650091)China

Objective: To explore the proliferation inhibitory effect of quinones from Blaps rynchopetera defense secretion on colorectal tumor cell lines. Methods: Human colorectal cancer cell HT-29, human colorectal adenocarcinoma cell Caco-2 and normal human colon epithelial cell CCD841 were chosen for the evaluation of inhibitory activity of the main quinones of B. rynchopetera defense secretion, including methyl p-benzoquinone(MBQ), ethyl p-benzoquinone(EBQ), and methyl hydroquinone(MHQ), through methyl thiazolyl tetrazolium assay. The tumor-related factors, cell cycles, related gene expressions and protein levels were detected by enzyme-linked immunosorbent assy, flow cytometry, RT-polymerase chain reaction and Western blot, respectively. Results: MBQ, EBQ, and MHQ could significantly inhibit the proliferation of Caco-2, with half maximal inhibitory concentration(IC50) values of 7.04±0.88, 10.92±0.32, 9.35±0.83, HT-29, with IC50values of 14.90±2.71, 20.50±6.37, 13.90±1.30, and CCD841, with IC50values of 11.40±0.68, 7.02±0.44 and 7.83±0.05 μg/mL, respectively. Tested quinones can reduce the expression of tumor-related factors tumor necrosis factor α, interleukin(IL)-10, and IL-6 in HT-29 cells, selectively promote apoptosis, and regulate the cell cycle which can reduce the proportion of cells in the G1phase and increase the proportion of the S ***, tested quinones could up-regulate mRNA and protein expression of GSK-3β and APC, while down-regulate that of β-catenin, Frizzled1, c-Myc, and CyclinD1 in the Wnt/β-catenin pathway of HT-29 ***: Quinones from B. rynchopetera defense secretion could inhibit the proliferation of colorectal tumor cells and reduce the expression of related factors, which would be functioned by regulating cell cycle, selectively promoting apoptosis, and affecting Wnt/β-catenin pathway-related mRNA and protein expressions.

关键词： Blaps rynchopetera Chinese medicine quinones anti-colorectal tumor Wnt/β-catenin pathway

Plasma big endothelin-1 is an effective predictor for ventricular arrythmias and end-stage events in primary prevention implantable cardioverter-defibrillator indication patients

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Journal of Geriatric Cardiology 2020年第7期17卷 427-433页

作者： xiao-Yao LI Shuang ZHAO xiao-Han FAN Ke-Ping CHEN Wei HUA Zhi-Min liu xiao-di XUE Bin ZHOU Shu ZHANG Arrhythmia Center State Key Laboratory of Cardiovascular DiseaseFuwai HospitalNational Center for Cardiovascular DiseasesChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijingChina

Objective To investigate whether plasma big endothelin-1(ET-1) predicts ventricular arrythmias(VAs) and end-stage events in primary prevention implantable cardioverter-defibrillator(ICD) indication patigents. Methods In total, 207 patients fulfilling the inclusion criteria from Fuwai Hospital between January 2013 and December 2015 were retrospectively analyzed. The cohort was divided into three groups according to baseline plasma big ET-1 tertiles: tertile 1( 0.7 pmol/L, n = 70). The primary endpoints were VAs. The secondary endpoints were end-stage events comprising all-cause mortality and heart transplantation. Results During a mean follow-up period of 25.6 ± 13.9 months, 38(18.4%) VAs and 78(37.7%) end-stage events occurred. Big ET-1 was positively correlated with NYHA class(r = 0.165, P = 0.018), serum creatinine concentration(Scr;r = 0.147, P = 0.034), high-sensitivity C-reactive protein(hs-CRP;r = 0.217, P = 0.002), Lg NT-pro BNP(r = 0.463, P diastolic diameter(LVEDD;r = 0.234, P = 0.039) and negatively correlated with left ventricular ejection fraction(LVEF;r =-0.181, P = 0.032). Kaplan-Meier analysis showed that elevated big ET-1 was associated with increased risk of VAs and end-stage events(P dication patients, plasma big ET-1 levels can predict VAs and end-stage events and may facilitate ICD-implantation risk stratification.

关键词： Big endothelin-1 End-stage events Implantable cardioverter-defibrillator implantation Primary prevention Ventricular arrythmias

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内冲击激发式安全雷管的研制与适配性测试

火工品 2025年第1期 1-6页

作者：邓小娟代鹏举董金龙刘晓娟王爱上海鲲程电子科技有限公司上海201100 广东宏大民爆集团有限公司广东广州510000 湖南湘科控股集团有限公司湖南长沙410000 江西新余国泰特种化工有限责任公司江西新余338000 湖南神斧集团湘南爆破器材有限责任公司湖南永州425000

针对工业雷管在使用过程中因起爆药感度高而引发的安全事故和环境污染问题,研制了一种新型内冲击激发式安全雷管(简称“IID雷管”),并开展其与不同点火药的适配性测试。通过采用猛炸药作为主要装药,以及设计激发组件,实现了高效有序的... 详细信息

针对工业雷管在使用过程中因起爆药感度高而引发的安全事故和环境污染问题,研制了一种新型内冲击激发式安全雷管(简称“IID雷管”),并开展其与不同点火药的适配性测试。通过采用猛炸药作为主要装药,以及设计激发组件,实现了高效有序的起爆和传爆过程;通过与不同点火药的适配测试,以及对起爆能力、延期精度等关键功能的全面评估,验证了IID雷管在与LTNR和K-K等药剂适配时,各项性能均满足行业标准。此外,IID雷管结构工艺简单、安全环保,具有广泛的应用前景。

关键词：安全雷管数码电子雷管内冲击激发无起爆药雷管激发组件

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Engineering SpyCatcher Variants with Proteolytic Sites for Less-Trace Ligation

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Chinese Journal of Chemistry 2019年第2期37卷 113-118,I0002页

作者： Xue-Jian Zhang Xia-Ling Wu Dong liu xiao-di Da xiao-Wei Wang Shuguang Yang Wen-Bin Zhang State Key Laboratory for Modification of Chemical Fibers and Polymer Materials Center for Advanced Low-dimension MaterialsCollege of Material Science and EngineeringDonghua UniversityShanghai 201620China Key Laboratory of Polymer Chemistry & Physics of Ministry of Education Center for Soft Matter Science and EngineeringCollege of Chemistry and Molecular EngineeringPeking UniversityBeijing 100871China

Summary of main observation and conclusion The SpyTag/SpyCatcher reaction is a powerful tool for bioconjugation, but it leaves a complex of considerable size after ligation. To facilitate removal of the catalytic fragment, proteolytic recog nit ion sites (such as DDDDK, AVLQ, and WELQ) were directly engineered into the first or second loop of SpyCatcher at locations after the reactive lysine to give a set of cleavable SpyCatcher variants. Among them, SpyCatcherDDDDK exhibits excellent reactivity with SpyTag and could still be cleaved proteolytically by enterokinase after ligation. Notably, SpyCatcherDDDDK is disordered in solution and forms an ordered complex upon reaction with SpyTag with a second order rate constant of 99.2 ± 0.1M^-1·S^-1 which is comparable to, if not faster than, most click reactions. The results demonstrate the high sequence plasticity of SpyCatcher and suggest that covalent bond formation may confer robustness on the folded structure against extensive mutation. These variants add to the expanding toolbox of genetically-encoded peptide-protein chemistry with diverse features.

关键词： SpyCatcher Variants Proteolytic

电刺激诱导miR-741-3p调控Radil促进施万细胞的迁移

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中国组织工程研究 2025年第19期29卷 4038-4043页

作者：刘庆高博杨霄姜宇王培承德医学院附属医院河北省承德市067000 承德医学院河北省承德市067000

背景:越来越多的动物实验和临床研究证实电刺激可以促进周围神经损伤修复,具体的机制尚未完全明确。目的:探讨电刺激诱导miR-741-3p调控Radil对施万细胞迁移的影响。方法:①12只雄性SD大鼠,随机分为电刺激组和对照组,电刺激组在坐骨神... 详细信息

背景:越来越多的动物实验和临床研究证实电刺激可以促进周围神经损伤修复,具体的机制尚未完全明确。目的:探讨电刺激诱导miR-741-3p调控Radil对施万细胞迁移的影响。方法:①12只雄性SD大鼠,随机分为电刺激组和对照组,电刺激组在坐骨神经挤压伤后连续电刺激7 d,对照组在坐骨神经挤压后不做任何处理。术后第7天取损伤处神经,利用荧光原位杂交技术检测两组miR-741-3p的表达差异。②通过miRDB、TargetScan和miRWalk数据库预测miR-741-3p靶基因。③将miR-741-3p模拟物及其对照、miR-741-3p抑制物及其对照、Radil siRNA及其对照、miR-741-3p抑制物+Radil siRNA及miR-741-3p抑制物+siRNA对照对施万细胞进行转染,采用RT-PCR检测转染效率,Transwell小室检测施万细胞的迁移能力。结果与结论:①电刺激组神经残端miR-741-3p荧光强度低于对照组;②数据库预测结果显示有69个基因可能是miR-741-3p靶基因,Radil是预测靶基因之一,主要参与细胞黏附和迁移;③与miR-741-3p抑制物对照组相比,miR-741-3p抑制物组施万细胞迁移数增多(Pdil siRNA组施万细胞迁移数减少(Pdil的表达水平升高;与miR-741-3p模拟物对照组相比,miR-741-3p模拟物组Radil的表达水平降低;⑤与miR-741-3p抑制物+siRNA对照组相比,miR-741-3p抑制物+Radil siRNA组施万细胞迁移数减少(Pdi的表达来促进施万细胞迁移。

关键词：施万细胞电刺激 miR-741-3p Radil 细胞迁移神经再生

New insight of obesity-associated NAFLD: Dysregulated “crosstalk” between multi-organ and the liver?

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Genes & diseases 2023年第3期10卷 799-812页

作者： Ya-di Wang Liang-Liang Wu xiao-Yan Qi Yuan-Yuan Wang Zhe-Zhen Liao Jiang-Hua liu Xin-Hua xiao Department of Metabolism and Endocrinology The First Affiliated HospitalHengyang Medical SchoolUniversity of South ChinaHengyangHunan 421001China Department of Gastrointestinal Surgery The First Affiliated HospitalHengyang Medical SchoolUniversity of South ChinaHengyangHunan 421001China

Obesity plays a crucial role in the development of non-alcoholic fatty liver disease (NAFLD). However, the underlying mechanism for the pathogenesis of obesity-associated NAFLD remains largely obscure. Although the “multiple hit” theory provides a more accurate explanation of NAFLD pathogenesis, it still cannot fully explain precisely how obesity causes NAFLD. The liver is the key integrator of the body's energy needs, receiving input from multiple metabolically active organs. Thus, recent studies have advocated the “multiple crosstalk” hypothesis, highlighting that obesity-related hepatic steatosis may be the result of dysregulated “crosstalk” among multiple extra-hepatic organs and the liver in obesity. A wide variety of circulating endocrine hormones work together to orchestrate this “crosstalk”. Of note, with deepening understanding of the endocrine system, the perception of hormones has gradually risen from the narrow sense (i.e. traditional hormones) to the broad sense of hormones as organokines and exosomes. In this review, we focus on the perspective of organic endocrine hormones (organokines) and molecular endocrine hormones (exosomes), summarizing systematically how the two types of new hormones mediate the dialogue between extra-hepatic organs and liver in the pathogenesis of obesity-related NAFLD.

关键词： Exosomes Hormones Non-alcoholic fatty liver disease(NAFLD) Obesity Organolkines

discrimination of foodborne pathogenic bacteria using synchrotron FTIR microspectroscopy

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Nuclear Science and Techniques 2017年第4期28卷 38-43页

作者： Ya-di Wang Xue-Ling Li Zhi-xiao liu Xing-Xing Zhang Jun Hu Jun-Hong Lü division of Physical Biology and CAS Key Laboratory of Interfacial Physics and Technology Shanghai Institute of Applied Physics Chinese Academy of Sciences (CAS)Shanghai 201800 China University of Chinese Academy of Sciences Beijing 100049China Shanghai Center for Bioinformation Technology Shanghai Academy of Science and Technology Shanghai 201203China Center for Clinical and Translational Medicine Shanghai Industrial Technology Institute Shanghai 201203 China

Traditional Fourier transform infrared(FTIR)spectroscopy has been recognized as a valuable method to characterize and classify kinds of *** this study,combined with multivariate statistical analysis,synchrotron radiation-based FTIR(SR-FTIR) microspectroscopy was applied to identify and discriminate ten foodborne bacterial *** results show that the whole spectra(3000-900 cm^(-1)) and three subdivided spectral regions(3000-2800,1800-1500 and 1200-900 cm^(-1),representing lipids,proteins and polysaccharides,respectively) can be used to type *** the whole spectra or the three subdivided spectra are good for discriminating the bacteria at levels of species and subspecies,but the whole spectra should be given preference at the genus *** findings demonstrate that SR-FTIR microspectroscopy is a powerful tool to identify and classify foodborne pathogenic bacteria at the genus,species and subspecies level.

关键词： Synchrotron FTIR microspectroscopy Foodborne pathogens Bacterial discrimination Subdivided spectral regions Multivariate statistical analysis

Modeling of Strain-Induced Precipitation Kinetics and Evolution of Austenite Grains in Nb Microalloyed Steels

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Journal of Iron and Steel Research International 2008年第3期15卷 65-69页

作者： ZHOU xiao-guang liu Zhen-yu YUAN Xiang-qian WU di WANG Guo-dong liu Xiang-hua State Key Laboratory of Rolling Technology and Automation Northeastern University Shenyang 110004 Liaoning China

Considering the effect of strain and chemical composition onprecipitation behavior, new models for the start and end time of Nb（C,N） precipitation in austenite under the conditions of different temperatures and strains have been investigated for Nb microalloyed steel. The value of n in the precipitation kinetic equation has been determined by using the available experimental data in literature, which indicated that n is a constant and independent of temperature. The values of the start and end time of the predicted precipitation are compared with the experimental values. Calculated results are in good agreement with the experimental results. Also, the evolution of austenite grains before ferrite transformation is simulated by taking the effect of precipitation into consideration. The measured austenite grain size is in good agreement with predicted one prior to ferrite transformation.

关键词： strain-induced precipitation start time precipitation static recrystallization grain size

Fractional Dehn twists and modular invariants

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Science China Mathematics 2021年第8期64卷 1735-1744页

作者： xiao-Lei liu School of Mathematical Sciences Dalian University of TechnologyDalian 116024China

In this paper, we establish a relationship between fractional Dehn twist coefficients of Riemann surface automorphisms and modular invariants of holomorphic families of algebraic curves. Specially, we give a characterization of pseudo-periodic maps with nontrivial fractional Dehn twist coefficients. We also obtain some uniform lower bounds of non-zero fractional Dehn twist coefficients.

关键词： fractional Dehn twists pseudo-periodic maps modular invariants families of curves