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中药蝴蝶果UPLC指纹图谱及含量测定

药物分析杂志 2024年第2期44卷 233-241页

作者：江洁怡杨敏娟汤瑞茵李国卫肖观林张靖年胥爱丽广东省中医药工程技术研究院广东省中医药研究开发重点实验室广州510095 广州中医药大学第五临床医学院广州510095 广东一方制药有限公司佛山528244

目的:建立中药蝴蝶果超高效液相色谱(UPLC)指纹图谱及没食子酸、原儿茶酸、对羟基肉桂酸、杨梅苷、槲皮苷5个成分的定量分析方法,为中药蝴蝶果质量控制提供依据。方法:采用Waters CORTECS UPLC T3色谱柱(150 mm×2.1 mm,1.6μm),以乙腈-... 详细信息

目的:建立中药蝴蝶果超高效液相色谱(UPLC)指纹图谱及没食子酸、原儿茶酸、对羟基肉桂酸、杨梅苷、槲皮苷5个成分的定量分析方法,为中药蝴蝶果质量控制提供依据。方法:采用Waters CORTECS UPLC T3色谱柱(150 mm×2.1 mm,1.6μm),以乙腈-0.1%磷酸水溶液为流动相,梯度洗脱,柱温30℃,流速0.20 mL·min^(-1),检测波长300 nm,进样量为1μL;采用“中药色谱指纹图谱相似度评价系统”生成对照指纹图谱并进行相似度分析,结合聚类分析、TOPSIS多元统计分析法进行评价。结果:建立了中药蝴蝶果UPLC指纹图谱,共标示了14个共有峰,指认了其中没食子酸、原儿茶酸、对羟基肉桂酸、杨梅苷、槲皮苷5个成分,并建立了该5个成分的定量分析方法。10批次中药蝴蝶果的指纹图谱相似度均在0.9以上,其中来源于江西的样品S9聚为Ⅰ类,质量最佳;来源于广西和广东的样品聚为Ⅱ类,质量次之;来源于江西的样品S1、S4、S6聚为Ⅲ类,质量最差。10批样品中上述5个成分的含量分别为0.236~0.356、0.118~0.398、0.108~0.141、0.146~0.222、0.046~0.104 mg·g^(-1)。结论:所建立的蝴蝶果指纹图谱及含量测定分析方法精密度高,稳定性好,可用于中药蝴蝶果的质量控制及评价。

关键词：中药蝴蝶果指纹图谱多成分含量测定聚类分析 TOPSIS多元统计分析

玉米雄性不育突变体ms1153的遗传分析与基因定位

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玉米科学 2024年第2期32卷 16-22页

作者：郭瑶晴孙晓靖连玉杰陈慧曹旭东陆涵孙华越汤继华陈晓阳河南农业大学农学院郑州450046 周口市农业科学院河南周口466000

以玉米雄性不育突变体ms1153为材料,通过减数分裂期花粉母细胞染色体观察和不同发育时期花药石蜡切片分析突变体不育表型产生的原因,利用F1和F2群体确定突变体ms1153的遗传方式和基因MS1153物理位置。结果表明,与野生型相比,突变体植株... 详细信息

以玉米雄性不育突变体ms1153为材料,通过减数分裂期花粉母细胞染色体观察和不同发育时期花药石蜡切片分析突变体不育表型产生的原因,利用F1和F2群体确定突变体ms1153的遗传方式和基因MS1153物理位置。结果表明,与野生型相比,突变体植株在营养生长期无明显差异,在生殖生长阶段花药不能从颖壳外露,成熟花粉粒内无淀粉积累。细胞学分析发现,突变体减数分裂过程正常,但减数分裂后绒毡层降解推迟。遗传分析表明,突变体ms1153的不育性状受1对隐性核基因控制。利用图位克隆的策略将MS1153基因定位于玉米第4染色体分子标记8243与8275之间,物理区间为221.4~226.2 Mb,此区间内没有已克隆雄性育性基因,说明MS1153是一个新的玉米雄性育性基因。

关键词：玉米雄性不育 ms1153 基因定位绒毡层

ovel Mutations in SCN4A Gene Cause Myotonia Congenita with Scoliosis

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Chinese Medical Journal 2018年第4期131卷 477-479页

作者： Yang-qi xu xiao-Li Liu xiao-Jun Huang WO-Tu Tian Hui-Dong tang Li Cao Department of Neurology Ruijin Hospital Shanghai Jiao Tong University Shanghai 200025 China Department of Neurology Shanghai Fengxian District Central Hospital Shanghai Jiao Tong University Affiliated Sixth People's Hospital South Campus Shanghai 201499 China Department of Neurology Ruijin Hospital North Shanghai Jiao Tong University Shanghai 201800 China

Myotonia congenita （MC） is a group of genetically and clinically heterogeneous congenital neuromuscular channelopathies, typically characterized by the delayed relaxation of the muscles after voluntary contraction, ... 详细信息

关键词： CHANNEL

The d-orbital coupling modulation of CuNi alloy for acetonitrile electrochemical reduction and in-situ hydrogenation behavior characterization

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Science China Chemistry 2023年第11期66卷 3242-3251页

作者： Boling Liu Dong He Zunjian Ke Hongbo Wang Chongyang tang qi Zhang Hang xu Menghua Yang Yafei Yang qi Liu Xiangheng xiao School of Physics and Technology Wuhan UniversityWuhan 430072China Wuhan Research Center for Infectious Diseases and Cancer Chinese Academy of Medical SciencesWuhan 430072China Department of Physics City University of Hong KongHong Kong 999077China

Electrochemical reduction of acetonitrile to ethylamine with a high selectivity is a novel approach to manufacture valuable primary amines which are important raw material in organic chemical industry. However, the poor ethylamine Faradic efficiency(FE_(ethylamine)) and catalyst stability at the high current density prohibit this method from being practically used. Herein, CuNi alloy ultrafine-nano-particles based on the d-orbital coupling modulation were synthesized through the electrodeposition and their catalytic performance towards acetonitrile reduction reaction(ACNRR) has been systematically studied. The highest FE_(ethylamine)(97%) is achieved with the current density of-114 mA cm^(-2). For practical application, the current density can reach-602.8 mA cm^(-2) with 82.8% FE_(ethylamine)maintained. With the appearance of other organics which co-exist with acetonitrile in the SOHIO process, CuNi can also hydrogenate acetonitrile in it with more than 80% FE_(ethylamine). Our in-situ spectroscopy analysis and DFT calculations towards the acetonitrile hydrogenation behavior reveal that the evenly dispersed Ni in Cu modulates the dband so as to endow CuNi with the better acetonitrile adsorption, milder binding energy with the reaction intermediates, smaller barrier for *CH_3CH_2NH_2 desorption and higher ability for H_2O dissociation to provide *H.

关键词： CuNi alloy acetonitrile reduction reaction d-orbital coupling modulation in-situ spectroscopy analysis acetonitrile adsorption

A modified creep index and its application to viscoplastic modelling of soft clays

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Journal of Zhejiang University-Science A(Applied Physics & Engineering) 2014年第4期15卷 272-281页

作者： qi-yin ZHU Ze-xiang WU Yan-ling LI Chang-jie xu Jian-hua WANG xiao-he XIA Department of Civil Engineering Shanghai Jiao Tong University Shanghai 200240 China School of Civil Engineering and Architecture East China Jiaotong University Nanchang 330013 China Research Center of Coastal and Urban Geotechnical Engineering Zhejiang University Hangzhou 310058 China

Conventional consolidation tests on reconstituted specimens of numerous natural soft clays show a decreasing of creep index Cae with increasing soil density. Based on all selected and conducted experimental results, a modified creep index Cae＊ defined in double logarithmic plane lge-lgt, was plotted for various clays, from which Cae＊ can be assumed as a constant for different soil densities. Then, the modified creep index was applied to a newly developed elastic viscoplastic model. In this way, the modified creep index Ca＊ can naturally take into account the nonlinear Cae revealing the influence of soil density in the soil assemblies without additional parameters. Finally, the enhanced model was incorporated into the finite element code ABAQUS and used to simulate a consolidation test and a test embankment. The improvement of simulations by the modified creep index was highlighted by comparing simulations using the conventional creep index Cae.

关键词： Clays Creep Consolidation test Embankment Finite element method Viscoplasticity

The effects of calcium combined with chitosan amendment on the bioavailability of exogenous Pb in calcareous soil

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Journal of Integrative Agriculture 2020年第5期19卷 1375-1386页

作者： CHANG Hui-qing WANG qi-zhen LI Zhao-jun WU jie xu xiao-feng SHI Zhao-yong Henan University of Science and Technology Luoyang 471000P.R.China Key Laboratory of Plant Nutrition and Fertilizer Ministry of Agriculture and Rural Affairs/China-New Zealand Joint Laboratory for Soil Molecular EcologyInstitute of Agricultural Resources and Regional PlanningChinese Academy of Agricultural SciencesBeijing 100081P.R.China

Lead(Pb) in soil may accumulate in crops and enter the human body. This study aimed to understand the speciation transformation and accumulation characteristics of exogenous Pb in calcareous soil with or without the application of soil amendments. Field experiments with continuous maize cultivation have been carried out for two years. The results showed that the contents of total Pb were slightly lower in 2016 than in 2015 for the same treatments;however, no significant difference between the years was observed. Soil Pb existed mainly in the residual fraction without exogenous Pb addition, and its proportion was more than 33% of the total soil Pb in the control and Ca treatments. When Pb was added to calcareous soil, Pb existed largely in the oxidizable and reducible fractions during the two-year experimental period, and those fractions made up over 83% of the total Pb. The proportion of the water-soluble Pb, regardless of the addition of Pb, was the lowest and was less than 0.0019% in all treatments, but the addition of Ca and chitosan amendments reduced the water-soluble and exchangeable Pb contents. The Pb content in different parts of maize followed the order root>leaf>stem>grain during the experimental period. Although maize had low transfer and enrichment factors in calcareous soil, which make the Pb content in the grain show no significant difference among the five treatments in the same year, adding Ca and chitosan to calcareous soil can reduce the Pb contents of maize, especially reduce the Pb contents of root, stem and leaf. Therefore, the addition of calcium and chitosan is an effective strategy for reducing Pb availability in calcareous soils.

关键词： exogenous Pb maize calcareous soil speciation transformation amendments

Unfractionated heparin attenuates endothelial barrier dysfunction via the phosphatidylinositol-3 kinase/serine/threonine kinase/nuclear factor kappa-B pathway

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Chinese Medical Journal 2020年第15期133卷 1815-1823页

作者： Sheng-Tian Mu jie tang Jian-qi Ma Yu Zhong Han-Zhe Liu xiao-Chun Ma Zhen Zheng Department of Intensive Care Unit Cancer Hospital of China Medical UniversityLiaoning Cancer Hospital and InstituteShenyangLiaoning 110042China Department of Intensive Care Unit The First Affiliated Hospital of China Medical UniversityShenyangLiaoning 110001China

Background::Vascular endothelial dysfunction is considered a key pathophysiologic process for the development of acute lung *** this study,we aimed at investigating the effects of unfractionated heparin(UFH)on the lipopolysaccharide(LPS)-induced changes of vascular endothelial-cadherin(VE-cadherin)and the potential underlying ***::Male C57BL/6 J mice were randomized into three groups:vehicle,LPS,and LPS+UFH *** injection of 30 mg/kg LPS was used to induce *** in the LPS+UFH group received subcutaneous injection of 8 U UFH 0.5 h before LPS *** lung tissue of the mice was collected for assessing lung injury by measuring the lung wet/dry(W/D)weight ratio and observing histological *** pulmonary microvascular endothelial cells(HPMECs)were cultured and used to analyze the effects of UFH on LPS-or tumor necrosis factor-alpha(TNF-α)-induced vascular hyperpermeability,membrane expression of VE-cadherin,p120-catenin,and phosphorylated myosin light chain(p-MLC),and F-actin remodeling,and on the LPS-induced activation of the phosphatidylinositol-3 kinase(PI3K)/serine/threonine kinase(Akt)/nuclear factor kappa-B(NF-κB)signaling ***::In vivo,UFH pretreatment significantly attenuated LPS-induced pulmonary histopathological changes(neutrophil infiltration and erythrocyte effusion,alveolus pulmonis collapse,and thicker septum),decreased the lung W/D,and increased protein concentration(LPS ***+UFH:0.57±0.04 vs.0.32±0.04 mg/mL,P=0.0092),total cell count(LPS ***+UFH:9.57±1.23 vs.3.65±0.78×105/mL,P=0.0155),polymorphonuclear neutrophil percentage(LPS ***+UFH:88.05%±2.88%vs.22.20%±3.92%,P=0.0002),and TNF-α(460.33±23.48 vs.189.33±14.19 pg/mL,P=0.0006)in the bronchoalveolar lavage *** vitro,UFH pre-treatment prevented the LPS-induced decrease in the membrane expression of VE-cadherin(LPS ***+UFH:0.368±0.044 vs.0.716±0.064,P=0.0114)and p120-catenin(LPS ***+UFH:0.208±0.018 vs.0.924±0.092,P=0.0

关键词： Acute lung injury Cadherin Capillary permeability Heparin

超声心动图对CHD患者中重度狭窄的诊断价值及与心功能分级相关性研究

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心血管康复医学杂志 2024年第3期33卷 313-318页

作者：徐萍萍唐琪张子宁郭强杨小峰江阴市中医院超声医学科江苏无锡214400

目的:探究超声心动图指标对冠心病(CHD)患者中重度狭窄的诊断价值及其与心功能分级相关性。方法:选择2020年12月至2022年12月期间本院收治的90例CHD患者纳入CHD组;根据NYHA分级将CHD组患者分为Ⅰ~Ⅱ级组(n=38),Ⅲ~Ⅳ级组(n=52)。根据Gen... 详细信息

目的:探究超声心动图指标对冠心病(CHD)患者中重度狭窄的诊断价值及其与心功能分级相关性。方法:选择2020年12月至2022年12月期间本院收治的90例CHD患者纳入CHD组;根据NYHA分级将CHD组患者分为Ⅰ~Ⅱ级组(n=38),Ⅲ~Ⅳ级组(n=52)。根据Gensini评分分为轻度组(n=35)和中重度组(n=55)。另外选择同期于本院体检的90例健康体检者作为对照组。采用彩色超声诊断仪检测两组LVEF、LVEDd和LVESd。对比对照组和CHD组、不同心功能分级和不同冠脉狭窄程度组心功能指标;采用ROC曲线分析上述心功能指标对CHD中重度狭窄的诊断价值;采用Spearman相关分析法分析CHD患者LVEF、LVEDd、LVESd、不同狭窄程度与NYHA分级的相关性。结果:与对照组相比,CHD组LVEF显著降低,LVEDd和LVESd显著升高,P均=0.001;与轻度组相比,中重度组LVEF[(45.31±5.08)%比(40.34±3.01)%]显著降低,LVEDd[(53.92±5.09)mm比(61.68±4.79)mm]和LVESd[(52.72±4.72)mm比(58.06±3.50)mm]显著升高,P均=0.001;与Ⅰ~Ⅱ级组相比,Ⅲ~Ⅳ级组LVEF[(45.07±4.95)%比(40.23±3.06)%]显著降低,LVEDd[(54.50±5.30)mm比(61.71±4.91)mm]和LVESd[(52.92±4.63)mm比(58.22±3.43)mm]显著更高,P均=0.001。LVEF、LVEDd和LVESd联合诊断CHD中重度狭窄的AUC为0.909,显著高于三种参数单独检测的(0.733、0.787、0.789),(Z=2.925、2.125、2.043,P<0.05或<0.01)。Spearman相关性分析结果显示,LVEF与CHD患者NYHA分级呈显著负相关(r=-0.514),LVEDd和LVESd与其呈显著正相关(r=0.538、0.546,P均=0.001),CHD不同冠脉狭窄程度与NYHA分级呈显著正相关(r=0.875,P=0.001)。结论:LVEF、LVEDd和LVESd联合检测对CHD患者中重度狭窄具有较高的临床诊断价值,与其心功能分级显著相关,可作为临床CHD患者冠脉狭窄程度的评估方法之一。

关键词：冠心病超声心动描记术,多普勒,彩色冠状动脉狭窄 NYHA心功能分级

Lactic Acid Reduces LPS-Induced TNF-a and IL-6 mRNA Levels Through Decreasing I?Ba Phosphorylation

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Journal of Integrative Agriculture 2013年第6期12卷 1073-1078页

作者： xu Guang-yong JIANG Jin-qi WANG Ming LI jie SU Jing-liang REN xiao-ming Animal Science and Technology College Beijing University of Agriculture Beijing Key Laboratory of Traditional Chinese Veterinary Medicine Beijing University of Agriculture College of Veterinary Medicine China Agricultural University

This study explored the effects over time of lactic acid （LA） on IκBα phosphorylation and nuclear factor-kappa B （NF-κB） p65 protein expression, and on tumor necrosis factor a （TNF-α） and interleukin-6 （IL-6） mRNA levels in rat intestinal mucosa microvascular endothelial cells （RIMMVECs） stimulated by lipopolysaccharide （LPS）. I？Ba, phosphorylated IκBa （p-IκBa） and p65 protein levels were monitored by Western blot analysis, and TNF-a and IL-6 mRNA levels were analyzed using real-time PCR. LA treatment reduced TNF-a and IL-6 mRNA levels in LPS-stimulated RIMMVECs, with the greatest effect being after 3 h. The highest inhibitory effect of LA on IκBa phosphorylation to prevent activation of NF-κB was after 6 h. These results suggest that LA reduces TNF-a and IL-6 mRNA levels through decreasing IκBa phosphorylation and blocking the dissociation of IKK complex, which prevents activation of NF-κB.

关键词： lactic acid TNF-a IL-6 IκBa NF-κB