检索结果-南通市图书馆

Inactivated H9N2 vaccines developed with early strains do not protect against recent H9N2 viruses:Call for a change in H9N2 control policy

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative Agriculture 2024年第6期23卷 2144-2148页

作者： Yanjing Liu Qingqing Yu Xiangyu Zhou Wenxin Li Xinwen He Yan Wang Guohua Deng Jianzhong Shi Guobin Tian xianying zeng Hualan Chen College of Veterinary Medicine Gansu Agricultural UniversityLanzhou 730070China State Key Laboratory for Animal Disease Control and Prevention Key Laboratory for Prevention and Control of Avian Influenza and Other Major Poultry DiseasesMinistry of Agriculture and Rural Affairs/Harbin Veterinary Research InstituteChinese Academy of Agricultural SciencesHarbin 150009China National Poultry Laboratory Animal Resource Center Harbin Veterinary Research InstituteChinese Academy of Agricultural SciencesHarbin 150009China Harbin Weike Biotechnology Co. Ltd.Harbin 150009China

H9N2 virus has been widely distributed in wild birds and poultry around the world since its first emergence in the United States of America in 1966(Gu et al.2017;Carnaccini and Perez 2020).The virus appeared in chickens in China in the early 1990s,and over the last two decades has gradually become the dominant epidemic subtype(Sun and Liu 2015;Bi et al.2020).Although H9N2 virus infection alone cannot cause severe disease or death in poultry,H9N2 virus-infected birds experience a degree of egg production drop and can be easily infected by other pathogens,thus causing economic losses for poultry industry.

关键词： H9N2 poultry policy

M6PR interacts with the HA2 subunit of influenza A virus to facilitate the fusion of viral and endosomal membranes

在线全文

学校读者我要写书评

暂无评论

Science China(Life Sciences) 2024年第3期67卷 579-595页

作者： Yuzhen Hu Li Jiang Guangwen Wang Yangming Song Zhibo Shan Xuyuan Wang Guohua Deng Jianzhong Shi Guobin Tian xianying zeng Liling Liu Hualan Chen Chengjun Li State Key Laboratory for Animal Disease Control and Prevention Harbin Veterinary Research InstituteChinese Academy of Agricultural SciencesHarbin 150069China

Influenza A virus(IAV) commandeers numerous host cellular factors for successful replication. However, very few host factors have been revealed to be involved in the fusion of viral envelope and late endosomal membranes. In this study, we identified cation-dependent mannose-6-phosphate receptor(M6PR) as a crucial host factor for the replication of IAV. We found that siRNA knockdown of M6PR expression significantly reduced the growth titers of different subtypes of IAV, and that the inhibitory effect of M6PR siRNA treatment on IAV growth was overcome by the complement of exogenously expressed M6PR. When A549 cells were treated with siRNA targeting M6PR,the nuclear accumulation of viral nucleoprotein(NP) was dramatically inhibited at early timepoints post-infection, indicating that M6PR engages in the early stage of the IAV replication cycle. By investigating the role of M6PR in the individual entry and post-entry steps of IAV replication, we found that the downregulation of M6PR expression had no effect on attachment, internalization, early endosome trafficking,or late endosome acidification. However, we found that M6PR expression was critical for the fusion of viral envelope and late endosomal membranes. Of note, M6PR interacted with the hemagglutinin(HA) protein of IAV, and further studies showed that the lumenal domain of M6PR and the ectodomain of HA2 mediated the interaction and directly promoted the fusion of the viral and late endosomal membranes,thereby facilitating IAV replication. Together, our findings highlight the importance of the M6PR–HA interaction in the fusion of viral and late endosomal membranes during IAV replication.

关键词： influenza A virus M6PR HA membrane fusion late endosome

Vaccination of poultry successfully eliminated human infection with H7N9 virus in China

在线全文

学校读者我要写书评

暂无评论

Science China(Life Sciences) 2018年第12期61卷 1465-1473页

作者： xianying zeng Guobin Tian Jianzhong Shi Guohua Deng Chengjun Li Hualan Chen State Key Laboratory of Veterinary Biotechnology Harbin Veterinary Research InstituteChinese Academy of Agricultural SciencesHarbin 150069China

The H7 N9 viruses that emerged in China in 2013 were nonpathogenic in chickens but mutated to a highly pathogenic form in early 2017 and caused severe disease outbreaks in chickens. The H7 N9 influenza viruses have caused five waves of human infection, with almost half of the total number of human cases(766 of 1,567) being reported in the fifth wave, raising concerns that even more human infections could occur in the sixth wave. In September 2017, an H5/H7 bivalent inactivated vaccine for chickens was introduced, and the H7 N9 virus isolation rate in poultry dropped by 93.3% after vaccination. More importantly,only three H7 N9 human cases were reported between October 1, 2017 and September 30, 2018, indicating that vaccination of poultry successfully eliminated human infection with H7 N9 virus. These facts emphasize that active control of animal disease is extremely important for zoonosis control and human health protection.

关键词： H7N9 influenza virus evolution vaccination human infection elimination

在线全文

学校读者我要写书评

暂无评论

A型流感病毒:新型内化受体的发现

中国兽医科学 2024年第8期54卷 1135-1135页

作者： Zixin Ni Jinliang Wang Xiaofei Yu Yifan Wang Jingfei Wang Xijun He Chengjun Li Guohua Deng Jianzhong Shi Huihui Kong Yongping Jiang Pucheng Chen xianying zeng Guobin Tian Hualan Chen Zhigao Bu State Key Laboratory for Animal Disease Control Harbin Veterinary Research Institute Chinese Academy of Agricultural Sciences Harbin People’s Republic of China

A型流感病毒(IAV)已发生多次大流行,造成了严重的危害。仅1918年的西班牙流感就造成了约5000万人的死亡,近年来,H7和H5亚型高致病性流感的爆发更造成了全球范围内逾1000例患者以及约4.2亿只家禽的死亡。IAV为分节段的单股负链RNA病毒,... 详细信息

A型流感病毒(IAV)已发生多次大流行,造成了严重的危害。仅1918年的西班牙流感就造成了约5000万人的死亡,近年来,H7和H5亚型高致病性流感的爆发更造成了全球范围内逾1000例患者以及约4.2亿只家禽的死亡。IAV为分节段的单股负链RNA病毒,病毒颗粒呈球形或丝状,直径约为80~120 nm。病毒由囊膜、基质蛋白及核心组成。其核心为结合病毒单股负链RNA的RNA聚合酶复合物,基质蛋白构成了病毒的外壳骨架,囊膜包含两种重要的糖蛋白,即血凝素(HA)和神经氨酸酶(NA)。NA的作用主要是协助释放病毒颗粒促其黏附于呼吸道上皮细胞,HA结合细胞受体,在病毒侵入细胞的过程中起着重要的作用。

关键词： A型流感病毒病毒颗粒基质蛋白细胞受体负链RNA病毒 H5亚型呼吸道上皮细胞高致病性

Glycosylation and an amino acid insertion in the head of hemagglutinin independently affect the antigenic properties of H5N1 avian influenza viruses

在线全文

学校读者我要写书评

暂无评论

Science China(Life Sciences) 2019年第1期62卷 76-83页

作者： Chunyang Gu xianying zeng Yangming Song Yanbing Li Liling Liu Yoshihiro Kawaoka Dongming Zhao Hualan Chen State Key Laboratory of Veterinary Biotechnology Harbin Veterinary Research Institute Chinese Academy of Agricultural Sciences Division of Virology Department of Microbiology and Immunology Institute of Medical Science University of Tokyo

Antigenic drift forces us to frequently update influenza vaccines; however, the genetic basis for antigenic variation remains largely unknown. In this study, we used clade 7.2 H5 viruses as models to explore the molecular determinants of influenza virus antigenic variation. We generated eight monoclonal antibodies(MAbs) targeted to the hemagglutinin(HA) protein of the index virus A/chicken/Shanxi/2/2006 and found that two representative antigenically drifted clade 7.2 viruses did not react with six of the eight MAbs. The E131 N mutation and insertion of leucine at position 134 in the HA protein of the antigenically drifted strains eliminated the reactivity of the virus with the MAbs. We also found that the amino acid N131 in the H5 HA protein is glycosylated. Our results provide experimental evidence that glycosylation and an amino acid insertion or deletion in HA influence antigenic variation.

关键词： Influenza virus H5N1 antigenic variation genetic basis

H7N9 virus infection triggers lethal cytokine storm by activating gasdermin E-mediated pyroptosis of lung alveolar epithelial cells

在线全文

学校读者我要写书评

暂无评论

National Science Review 2022年第1期9卷 47-55页

作者： Xiaopeng Wan Jiqing Li Yupeng Wang Xiaofei Yu Xijun He Jianzhong Shi Guohua Deng xianying zeng Guobin Tian Yanbing Li Yongping Jiang Yuntao Guan Chengjun Li Feng Shao Hualan Chen State Key Laboratory of Veterinary Biotechnology Harbin Veterinary Research Institute Chinese Academy of Agricultural Sciences National Institute of Biological Sciences

The H7 N9 influenza virus emerged in China in 2013,causing more than 1560 human infections,39% of which were fatal.A ’cytokine storm’ in the lungs of H7 N9 patients has been linked to a poor prognosis and death;however,the underlying mechanism that triggers the cytokine storm is ***,we found that efficient replication of the H7 N9 virus in mouse lungs activates gasdermin E(GSDME)-mediated pyroptosis in alveolar epithelial cells,and that the released cytosolic contents then trigger a cytokine *** of Gsdme switched the manner of death of A549 and human primary alveolar epithelial cells from pyroptosis to apoptosis upon H7 N9 virus infection,and Gsdme knockout mice survived H7 N9 virus lethal *** findings reveal that GSDME activation is a key and unique mechanism for the pulmonary cytokine storm and lethal outcome of H7 N9 virus infection and thus opens a new door for the development of antivirals against the H7 N9 virus.

关键词： H7N9 virus cytokine storm gasdermin E pyroptosis

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

血栓弹力图检测用于肝癌凝血状态评估的价值

中国实用医刊 2024年第7期51卷 90-93页

作者：商哲王焕成张茜闫小娴曾显莹李苗苗南阳市第一人民医院输血科南阳473000

目的分析血栓弹力图检测用于肝癌凝血状态评估的价值。方法分析性研究。抽取2020年1月至2022年12月南阳市第一人民医院收治的肝癌患者153例作为研究组,并以1∶1配比抽取同期肝血管瘤患者153例作为对照组。两组均行血栓弹力图检测和凝血... 详细信息

目的分析血栓弹力图检测用于肝癌凝血状态评估的价值。方法分析性研究。抽取2020年1月至2022年12月南阳市第一人民医院收治的肝癌患者153例作为研究组,并以1∶1配比抽取同期肝血管瘤患者153例作为对照组。两组均行血栓弹力图检测和凝血功能检测,比较研究组与对照组、初诊肝癌与复诊肝癌患者的血栓弹力图检测指标[凝血反应时间(R值)、血块形成时间(K值)、血块强度(MA值)、凝血综合指数(CI值)、凝固角(Angle值)]和凝血指标[凝血酶时间(TT)、凝血酶原时间(PT)、纤维蛋白原(FIB)、国际标准化比值(INR)]水平,分析不同治疗方式肝癌患者的血栓弹力图检测指标水平,评估不同检测方式对肝癌凝血状态的检出率。结果研究组R值、K值、PT、FIB、INR水平高于对照组,MA值、CI值、Angle值低于对照组(P<0.05)。复诊肝癌患者R值、K值高于初诊肝癌患者,MA值、CI值、Angle值、FIB水平低于初诊肝癌患者(P<0.05)。肝癌患者TACE治疗R值、K值高于保守治疗和肝切除,MA值、CI值、Angle值低于保守治疗和肝切除(P<0.05)。血栓弹力图血液低凝状态检出率(18.95%,29/153)高于凝血功能检测(7.19%,11/153),P<0.05。结论血栓弹力图检测能灵敏反映肝癌患者的凝血状态,有利于临床及时发现凝血功能异常情况,并采取针对性预防措施改善患者预后。

关键词：肝癌血栓弹力图凝血状态

Influenza A virus use of BinCARD1 to facilitate the binding of viral NP to importinα7 is counteracted by TBK1-p62 axis-mediated autophagy

在线全文

学校读者我要写书评

暂无评论

Cellular & Molecular Immunology 2022年第10期19卷 1168-1184页

作者： Xuyuan Wang Li Jiang Guangwen Wang Wenjun Shi Yuzhen Hu Bo Wang xianying zeng Guobin Tian Guohua Deng Jianzhong Shi Liling Liu Chengjun Li Hualan Chen College of Veterinary Medicine Gansu Agricultural UniversityLanzhou730070China Guangdong Laboratory for Lingnan Modern Agriculture Guangzhou510642China State Key Laboratory of Veterinary Biotechnology Harbin Veterinary Research InstituteChinese Academy of Agricultural SciencesHarbin150069China

As a major component of the viral ribonucleoprotein(vRNP)complex in influenza A virus(IAV),nucleoprotein(NP)interacts with isoforms of importinαfamily members,leading to the import of itself and vRNP complex into the nucleus,a process pivotal in the replication cycle of *** this study,we found that BinCARD1,an isoform of Bcl10-interacting protein with CARD(BinCARD),was leveraged by IAV for efficient viral ***1 promoted the nuclear import of the vRNP complex and newly synthesized NP and thus enhanced vRNP complex ***,we found that BinCARD1 interacted with NP to promote NP binding to importinα7,an adaptor in the host nuclear import ***,we also found that BinCARD1 promoted RIG-I-mediated innate immune signaling by mediating Lys63-linked polyubiquitination of TRAF3,and that TBK1 appeared to degrade *** showed that BinCARD1 was polyubiquitinated at residue K103 through a Lys63 linkage,which was recognized by the TBK1-p62 axis for autophagic ***,our data demonstrate that IAV leverages BinCARD1 as an important host factor that promotes viral replication,and two mechanisms in the host defense system are triggered—innate immune signaling and autophagic degradation—to mitigate the promoting effect of BinCARD1 on the life cycle of IAV.

关键词： Influenza A virus BinCARD1 TRAF3 TBK1 p62

Genetic and biological characteristics of the globally circulating H5N8 avian influenza viruses and the protective efficacy offered by the poultry vaccine currently used in China

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Science China(Life Sciences) 2022年第4期65卷 795-808页

作者： Pengfei Cui xianying zeng Xuyong Li Yanbing Li Jianzhong Shi Conghui Zhao Zhiyuan Qu Yanwen Wang Jing Guo Wenli Gu Qi Ma Yuancheng Zhang Weipeng Lin Minghui Li Jingman Tian Dongxue Wang Xin Xing Yanjing Liu Shuxin Pan Yaping Zhang Hongmei Bao Liling Liu Guobin Tian Chengjun Li Guohua Deng Hualan Chen State Key Laboratory of Veterinary Biotechnology Harbin Veterinary Research InstituteChinese Academy of Agricultural SciencesHarbin150069China College of Agronomy Liaocheng UniversityLiaocheng252000China Guangdong Laboratory for Lingnan Modern Agriculture Guangzhou510642China

The H5N8 avian influenza viruses have been widely circulating in wild birds and are responsible for the loss of over 33 million domestic poultry in Europe, Russia, Middle East, and Asia since January 2020. To monitor the invasion and spread of the H5N8 virus in China, we performed active surveillance by analyzing 317 wild bird samples and swab samples collected from 41,172 poultry all over the country. We isolated 22 H5N8 viruses from wild birds and 14 H5N8 viruses from waterfowls. Genetic analysis indicated that the 36 viruses formed two different genotypes: one genotype viruses were widely detected from different wild birds and domestic waterfowls;the other genotype was isolated from a whopper swan. We further revealed the origin and spatiotemporal spread of these two distinct H5N8 virus genotypes in 2020 and 2021. Animal studies indicated that the H5N8 isolates are highly pathogenic to chickens, mildly pathogenic in ducks, but have distinct pathotypes in mice. Moreover, we found that vaccinated poultry in China could be completely protected against H5N8 virus challenge. Given that the H5N8 viruses are likely to continue to spread in wild birds, vaccination of poultry is highly recommended in high-risk countries to prevent H5N8 avian influenza.

关键词： avian influenza virus H5N8 evolution pathogenicity antigenicity vaccine protective efficacy