检索结果-南通市图书馆

维普期刊数据库博看期刊

Deletions in the pyruvate pathway of Salmonella Typhimurium alter SPI1-mediated gene expression and infectivity

在线全文

学校读者我要写书评

暂无评论

Journal of Animal Science and Biotechnology 2013年第4期4卷 241-252页

作者： Jason Abernathy Carolina Corkill Carolee Hinojosa xianyao li Huaijun Zhou Department of Animal Science University of California Department of Poultry Science Texas A&M University College Station College of Animal Science and Technology Shandong Agricultural University

Background： Salmonella enter/ca serovar Typhimurium is a major foodborne pathogen worldwide. S. Typhimurium encodes type III secretion systems via Salmonella pathogenicity islands （SPI）, producing the major effector proteins of virulence. Previously, we identified two genes of Salmonella pyruvate metabolism that were up-regulated during chicken cell infection： pyruvate formate lyase I （pf/B） and b/functional acetaldehyde-CoA/alcohol dehydrogenase （adhE）. We were therefore interested in examining the role these genes may play in the transmission of Salmonella to humans. Methods： Mutant strains of Salmonella with single gene deletions for pflB and adhE were created. Invasion and growth in human HCT-8 intestinal epithelial cells and THP-1 macrophages was examined. Quantitative PCR was performed on 19 SPI-1 genes. Results： In HCT-8 cells, both mutant strains had significantly higher intracellular counts than the wild-type from 4 to 48 h post-infection. Various SPI-1 genes in the mutants were up-regulated over the wild-type as early as 1 h and lasting until 24 h post-infection. In THP-1 cells, no significant difference in internal Salmonella counts was observed; however, SPI-1 genes were largely down-regulated in the mutants during the time-course of infection. We also found five SPI-1 genes - hilA, hiIC hill）, sicP and rtsA - which were up-regulated in at least one of the mutant strains in log-phase broth cultures alone. We have therefore identified a set of SPI-1 virulence genes whose regulation is effected by the central metabolism of Salmonella.

关键词： adhE Metabolism pflB Pyruvate Salmonella pathogenicity island Salmonella Typhimurium Virulence

Development of silicon photonic devices for optical interconnects

在线全文

学校读者我要写书评

暂无评论

Science China Technological Sciences 2013年第3期56卷 586-593页

作者： XIAO Xi li ZhiYong CHU Tao XU Hao li xianyao NEMKOVA Anastasia KANG Xiong YU YuDe YU JinZhong State Key Laboratory on Integrated Optoelectronics Institute of SemiconductorsChinese Academy of Sciences

Silicon photonic devices based on complementary-metal-oxide-semiconductor(CMOS) compatible technologies have shown attractive performances on very-large-scale monolithic optoelectronic integration,high speed modulation and switching,and efficient off-chip optical *** paper presents the recent progress on fast silicon optical modulation,wavelength-insensitive optical switching and efficient optical coupling techniques in our *** CMOS-compatible silicon optical couplers with different structures have been developed,showing the highest coupling efficiency of 65%.Broadband silicon-based optical switches with sub-nanosecond switching on-off time are experimentally *** modulators with novel PN junctions are demonstrated with the speed up to 50 Gb s-1.

关键词： silicon photonics microresonator optical modulator optical switch

鸡脾脏METTL3、METTL14基因对肠炎沙门氏菌感染的表达调控

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

中国畜禽种业 2024年第1期20卷 13-18页

作者：赵亚男任艳茹谢宾王园美李显耀山东农业大学动物科技学院山东泰安271018 济宁市任城区畜牧兽医事业发展中心山东济宁272000

为探讨鸡METTL3、METTL14基因对肠炎沙门氏菌感染的调控作用,该试验将40只2日龄肠炎沙门氏菌阴性广西瑶鸡(父本)和济宁百日鸡(母本)杂交的F1代随机均分为2组,试验组和对照组分别接种0.3mL肠炎沙门氏菌菌液及PBS,在接种后第1天和第7天鸡... 详细信息

为探讨鸡METTL3、METTL14基因对肠炎沙门氏菌感染的调控作用,该试验将40只2日龄肠炎沙门氏菌阴性广西瑶鸡(父本)和济宁百日鸡(母本)杂交的F1代随机均分为2组,试验组和对照组分别接种0.3mL肠炎沙门氏菌菌液及PBS,在接种后第1天和第7天鸡的脾脏组织提取总RNA,采用实时荧光定量PCR检测不同时间点METTL3、METTL14基因的表达水平。结果显示:METTL3和METTL14具有相似的表达趋势,均在感染后第1天试验组高于对照组(P<0.05),感染后第7天试验组低于对照组,但只有METTL14差异显著(P<0.05)。可见,鸡感染肠炎沙门氏菌后,可以引起脾脏组织METTL3和METTL14基因的差异表达。

关键词： m^(6)A 肠炎沙门氏菌 METTL3 METTL14 基因表达

Development of silicon photonic devices for optical interconnects

在线全文

学校读者我要写书评

暂无评论

Chinese Science Bulletin 2013年第7期58卷 586-593页

关键词：光子器件光学互连开发金属氧化物半导体 CMOS兼容硅光耦合技术光电子集成

Study on Running Control System of Power- locomotive in Steel Factory

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Study on Running Control System of Power- locomotive in Stee...

Effect of norepinephrine on host immunity and bacterial infection

第一届国际测量仪器与测试自动化学术会议

作者： Meng xianyao Han Xinjie li Chunfeng (Dalian Maritime University. Dalian 116026 China)

＜正＞Nowadays in our country, the coke-carrying locomotive in the coal carbonization industry is predominantly driven by workers. In the way of manual operation, the accuracy of positioning is lower, the work is hard and the incidents of injury are greater. Confronted by this reality, a new control system is designed through contraposition and interlocking of three cars in the cokery locomotive. The key technologies and ways to run control of the positioning are studied. The technology of Radio Frequency Identification （RIFD） is used in the oven number identification. The three-lamp contraposition detecting device is used to make contraposition accurate, and the cokery locomotive is controlled based on the running curve, etc. In the control system studied in this paper, zero-error oven number identification can be achieved and the error of auto-position is within ±10mm. The dynamic characteristic of this system is good and the control of the system is flexible and reliable. The proposed control system solvs the problem of auto control of the coke-oven locomotive in the poor surroundings.

关键词： Cokery locomotive running control oven number identification precise positioning.

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Chinese Medical Journal 2024年第3期137卷 362-364页

作者： Jia Wang Zhiyao Fang Xue Dong Wenwen li xianyao Wan Department of Critical Care Medicine The First Affiliated Hospital of Dalian Medical UniversityDalianLiaoning 116021China

To the Editor:Norepinephrine(NE),which belongs to the catecholamine family,is increasingly produced by the sympathetic nervous system and adrenal medulla when the host is under *** has been demonstrated that NE has a significant anti-inflammatory *** addition,increasing evidence has indicated that stress hormones are directly involved in the interaction between bacteria and the host.A variety of bacteria can exhibit changes in growth,virulence,and biofilm by high concentrations of ***,this article aimed to summarize the main research developments regarding the effects of NE on host immunity and bacterial infection,as well as the attendant molecular mechanisms[Figure 1].

关键词： effect. Figure stress

辨证施护、耳穴压豆联合常规护理消渴病不寐症随机平行对照研究

在线全文

学校读者我要写书评

暂无评论

实用中医内科杂志 2014年第3期28卷 163-165页

作者：李先尧广西梧州市中医医院内二科广西梧州543004

[目的]观察辨证施护、耳穴压豆联合常规护理消渴病不寐症疗效。[方法]使用随机平行对照方法,将60例住院患者按随机数字表法分为两组。对照组30例常规护理。治疗组30例辨证施护结合耳穴压豆护理;辨证施护：①心脾两虚,压豆选穴：心、心... 详细信息

[目的]观察辨证施护、耳穴压豆联合常规护理消渴病不寐症疗效。[方法]使用随机平行对照方法,将60例住院患者按随机数字表法分为两组。对照组30例常规护理。治疗组30例辨证施护结合耳穴压豆护理;辨证施护：①心脾两虚,压豆选穴：心、心、枕、肺、脾、内分泌、神门、皮质下;护理干预：向阳病房,保持安静,多休息;避免思虑过多;忌黏腻、酒,适量食用动物心脏、莲子、黄芪等安神之品。②阴虚火旺：压豆选穴：心、肾、肝、神门、内分泌、枕、皮质下;护理干预：病房内空气流通,安慰和心理开导;足够休息,不能过度劳累,多喝水,忌油炸食物,多食莲子、百合等安神之品。耳穴压豆护理：选择圆形且表面光滑以及质地坚硬,对皮肤没有毒副作用的王不留行籽来作为贴压物。选择患者一侧耳部,耳廓固定,探棒从上至下在选择区域内找准穴位敏感点,留下压痕;消毒后将王不留行籽贴在此处,按压3－5s,胀、酸后止。2-3次/d,3-5min/次,睡前1h暂停按压。3d更换1次耳贴。常规护理同对照组。连续治疗12d为1疗程。观测临床症状、不良反应。连续治疗3疗程,判定疗效。[结果]治疗组治愈15例,显效10例,有效3例,无效2例,总有效率93.33%。对照组治愈10例,显效6例,有效9例,无效5例,总有效率83.33%。治疗组疗效优于对照组（P〈0.01）。[结论]辨证施护、耳穴压豆联合常规护理消渴病不寐症效果显著,值得推广。

关键词：消渴病不寐症辨证施护耳穴压豆随机平行对照研究

在线全文

学校读者我要写书评

暂无评论

鸡主要致死基因研究进展

中国家禽 2022年第2期44卷 96-99页

作者：杨娜陈月季李显耀山东农业大学动物科技学院山东泰安271018

雏鸡胚胎阶段不可避免地会出现死亡,遗传是造成鸡胚死亡的重要原因之一,在胚胎发育期,个体携带的有害基因将危害后代的生长发育,导致鸡胚或幼雏死亡,即使存活下来的个体也存在生理缺陷,影响鸡的生产性能及经济效益。文章综述了鸡中发现... 详细信息

雏鸡胚胎阶段不可避免地会出现死亡,遗传是造成鸡胚死亡的重要原因之一,在胚胎发育期,个体携带的有害基因将危害后代的生长发育,导致鸡胚或幼雏死亡,即使存活下来的个体也存在生理缺陷,影响鸡的生产性能及经济效益。文章综述了鸡中发现的致死基因及其研究现状,为鸡品种的选育及开发提供参考。

关键词：鸡致死基因胚胎

在线全文

学校读者我要写书评

暂无评论

世界博物馆发展趋势

科技导报 1985年第6期 44-46页

作者：黎先耀中国自然科学博物馆协会副理事长中国科普创作协会理事科普作家

不久前,我曾参加中国科学技术协会代表团,先后对英、美、法、联邦德国、瑞典、日本、加拿大等国的博物馆事业,作了比较深入的考察研究。总的印象,世界博物馆事业在恢复了第二次世界大战的创伤以后,适应现代社会发展的需要,正在不断地蓬... 详细信息

不久前,我曾参加中国科学技术协会代表团,先后对英、美、法、联邦德国、瑞典、日本、加拿大等国的博物馆事业,作了比较深入的考察研究。总的印象,世界博物馆事业在恢复了第二次世界大战的创伤以后,适应现代社会发展的需要,正在不断地蓬勃发展。八十年代确实可以说是一个博物馆事业兴旺发达的年代。世界博物馆的发展速度相当快,它表现在:数量的增加,内容的丰富,规模的扩大,分布的广泛,水平的提高等各方面。最近十几年来,全世界博物馆已由一两万座增加到两三万座。美国前些年平均三、四天就增加一座博物馆,现在已有博物馆近五千座,

关键词：博物馆发展国际博览会美国自然历史博物馆历史悠久讲解员