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包容性增长理念与西藏发展公共政策取向探讨

西藏大学学报（社会科学版） 2012年第3期27卷 46-49,85页

作者：梁爽崔先维吉林大学行政学院长春吉林130021

包容性增长理念是人们在反思经济增长与社会发展的关系基础上提出的一种理念,其形成和发展有着深刻的理论渊源和现实基础。包容性增长理念强调经济增长的包容性和社会发展的宽容性,与西藏追求长治久安和跨越式发展的目标具有高度一致性... 详细信息

包容性增长理念是人们在反思经济增长与社会发展的关系基础上提出的一种理念,其形成和发展有着深刻的理论渊源和现实基础。包容性增长理念强调经济增长的包容性和社会发展的宽容性,与西藏追求长治久安和跨越式发展的目标具有高度一致性。同时,作为一种理念,包容性增长需要以公共政策作为其实现的路径,而公共政策也只有以科学的理念作为指导,才能达到政策目标,实现维护社会公共利益的目的。

关键词：包容性增长公共政策社会公正政策取向

妊娠早期和晚期棕色脂肪组织代谢能力与基因表达变化

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中华内分泌代谢杂志 2023年第7期39卷 588-595页

作者：肖文潘怡慧仲红崔县伟江苏第二师范学院南京211200 南京医科大学附属妇产医院(南京市妇幼保健院)医学研究中心 210004

目的探究不同妊娠时期棕色脂肪组织(brown adipose tissue, BAT)的基因表达及代谢能力变化。方法建立C57BL/6J小鼠正常妊娠动物模型, 以同周龄未妊娠小鼠为对照组, 观察妊娠各时期BAT组织形态变化, 检测产热标志物解偶联蛋白1(UCP1)表... 详细信息

目的探究不同妊娠时期棕色脂肪组织(brown adipose tissue, BAT)的基因表达及代谢能力变化。方法建立C57BL/6J小鼠正常妊娠动物模型, 以同周龄未妊娠小鼠为对照组, 观察妊娠各时期BAT组织形态变化, 检测产热标志物解偶联蛋白1(UCP1)表达, 揭示脂肪棕色化及线粒体标志基因的mRNA水平变化;此外选取妊娠早期和晚期小鼠BAT进行转录组测序(RNA sequencing, RNA-seq), 筛选随妊娠进程的差异表达基因, 并开展基因本体(gene ontology, GO)和京都基因与基因组百科全书(Kyoto encyclopedia of genes and genomes, KEGG)分析。结果随妊娠进展, BAT脂滴显著增大, 产热标志蛋白UCP1表达下降(P<0.01), 脂肪棕色化标志基因Ucp1、2型脱碘酶(Dio2)、过氧化物酶增殖体激活受体γ辅激活因子1α(Pgc1α)以及线粒体标志基因细胞色素C(CytC)均显著下调(P<0.001), 以妊娠晚期组减弱最为显著。RNA-seq共筛选出差异表达基因1 298个, 其中906个基因在妊娠晚期上调、392个基因下调, GO及KEGG通路分析显示差异表达基因在脂质代谢、性类固醇激素、炎症因子等多种生物调节功能通路富集。结论小鼠妊娠晚期BAT出现脂滴变大、产热活性与代谢能力降低现象, BAT基因表达在妊娠不同时期存在显著差异, 因此BAT代谢能力降低可能是引发妊娠期代谢异常的重要原因。

关键词：妊娠棕色脂肪 RNA测序能量代谢

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胃癌DNA错配修复基因hMSH2突变研究

解放军医学杂志 2000年第2期25卷 125-126页

作者：刘晓茹崔娴维解放军第202医院

采用PCR、聚丙烯酰胺电泳、单链构象多态性分析及银染技术 ,检测 30例胃癌组织DNA微卫星不稳定 (MSI)及hMSH2基因突变情况。探讨错配修复 (MMR)基因与胃癌的关系及发生的分子机制。结果显示 30例胃癌中有 8例表现MSI ;有 5例 6次检测到h... 详细信息

采用PCR、聚丙烯酰胺电泳、单链构象多态性分析及银染技术 ,检测 30例胃癌组织DNA微卫星不稳定 (MSI)及hMSH2基因突变情况。探讨错配修复 (MMR)基因与胃癌的关系及发生的分子机制。结果显示 30例胃癌中有 8例表现MSI ;有 5例 6次检测到hMSH2基因突变 ,其中生殖细胞突变 2次 ,体细胞突变 4次。本研究结果认为 ,胃癌组织DNA中存在MSI和MMR基因突变。部分胃癌的发生机制可能为MMR基因缺陷导致基因组不稳定 ,产生基因突变而使细胞恶性转化。

关键词：胃肿瘤 DNA 修复基因突变 hMSH2基因

生命早期肠道菌群的建立通过编程脂肪细胞产热能力降低远期肥胖发生

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中华内分泌代谢杂志 2024年第10期40卷 872-878页

作者：方家丽龙雪仲红崔县伟南京医科大学附属妇产医院(南京市妇幼保健院)医学研究中心 210004 南京师范大学食品与制药工程学院 210023

目的探究生命早期肠道菌群的建立是否可以通过编程脂肪细胞产热能力影响远期肥胖发生的易感性。方法采用抗生素混合物(ABX)处理新生小鼠,经16S rRNA测序证实ABX处理引发生命早期肠道菌群建立紊乱。继续追踪上述小鼠在断奶时、成年后以... 详细信息

目的探究生命早期肠道菌群的建立是否可以通过编程脂肪细胞产热能力影响远期肥胖发生的易感性。方法采用抗生素混合物(ABX)处理新生小鼠,经16S rRNA测序证实ABX处理引发生命早期肠道菌群建立紊乱。继续追踪上述小鼠在断奶时、成年后以及高脂饮食状态下的体重、体脂含量,评估小鼠肥胖表型变化;通过分析小鼠血糖、血脂水平,开展葡萄糖耐量和胰岛素耐量试验,评估小鼠糖脂代谢能力变化;通过免疫组化、Western印迹法检测脂肪组织解偶联蛋白1(UCP1)的表达,评估小鼠脂肪细胞产热能力变化。结果16S rRNA测序显示ABX处理显著降低了新生小鼠肠道菌群的α多样性和β多样性,与未处理小鼠相比,ABX组小鼠菌群构成上差异显著,益生菌如毛螺菌(Lachnospiraceae_NK4A136_group)属NK4A136群、嗜黏蛋白阿克曼菌(Akkermansia muciniphila,AKK)等丰度显著降低。追踪监测发现,ABX处理并未影响小鼠哺乳期的体重以及体脂含量,小鼠体表温度尤其肩胛区显著降低,UCP1免疫组化和Western印迹结果显示脂肪组织产热能力受损。这种对脂肪组织产热能力的影响可以持续到成年,导致小鼠寒冷抵抗能力降低,但上述改变并未引发正常饮食下小鼠体重、体脂、血糖以及葡萄糖耐受性和胰岛素敏感性的改变。当更换高脂饮食后,ABX处理小鼠体重、体脂含量显著增长,血糖水平升高明显,提示小鼠更容易发生肥胖,进一步热成像、UCP1含量检测揭示这种肥胖易感性同样与脂肪组织产热能力受损有关。结论生命早期肠道菌群的建立是调控脂肪细胞产热能力的关键,扰乱健康菌群建立容易引发成年后高脂饮食诱导的肥胖。

关键词：生命早期肠道菌群脂肪组织产热活性肥胖

A fast and highly efficient hiPSCs-Adipocytes differentiation method:patient specific cell models for adipogenesis and obesity research

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A fast and highly efficient hiPSCs-Adipocytes differentiatio...

第十三届江浙沪儿科学术会议暨2016年浙江省医学会儿科学学术年会

作者： Chenbo Ji Yuhui Ni Yaping Zhao xianwei cui Lianghui You Xing Wang Jiwu Wang Xirong Guo Nanjing Maternity and Child Health Care Hospital

Obesity epidemic is a growing global health *** far there is no safe and effective way to prevent obesity,as weight loss does not simply depend on diet or physical *** is characterized by an excessive accumulation of adipose tissue,therefore understanding the biological features of adipocytes is one of the keys to find the cure for *** in vitro models,such as 3 T3-L1,primary preadipocytes or some adult multipotent stem cells,contribute to our understanding of adipogenesis and the mechanisms of ***,they are either murine origin cell lines,or primary cells withlimited expansion,which caused many contradictory *** present study,we developed an improved method to get human induced pluripotent stem cells(hiPSCs)drived mesenchymal stem cells(MSCs) with high capacity of adipocyte differention(80-95%efficiency) after one day adipocyte differentiation *** gene expression signatures of the adipocytes differentiated from the hiPSCs-MSCs is highly similar to primary mature adipocytes,with sensitive insulin response and function of adipokines *** previous study demonstrated that C10 orf116 and miR-148 a facilitated adipogenesis in primary preadipocyte or *** use these two factors to test the application of our newly developed hiPSCsMSCs-Adipocyte model in adipogenesis *** addition,by comparing iPSCs lines from donors with different body mass index(BMI),we found the efficiency of adipogenic differention were highly related to BMI of the *** conclusion,this hiPSCs-MSCs-Adipocyte model we developed is fast,highly efficient,and with patient specific genetic background,which should be a great tool for adipogenesis research and other obesity mechanism or clinical research.

关键词： hiPSCs adipogenesis Obesity

Antibacterial function and mechanism of peptide β-casein 65 from breast milk

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Antibacterial function and mechanism of peptide β-casein 65...

第十三届江浙沪儿科学术会议暨2016年浙江省医学会儿科学学术年会

作者： Jun Zhang Lianghui You Yanrong Fu Xiao Liu Xing Wang Yun Li xianwei cui Chenbo Ji Xirong Guo Department of Pediatrics Nanjing Maternal and Child Health Hospital Affiliated to Nanjing Medical University Maternal and Child Health Institute Nanjing Maternity and Child Health Care Hospital

Breast milk which has an effective function on preventing infection is the ideal food for newborns.β-casein65 is a novel endogenous peptides found in preterm milk by Liquid chromatography-mass spectrometry/mass spectrometry(LC-MS/MS) *** experiments confirm that β-casein65 has strong antibacterial activity on common newborn infection bacteria such as *** and *** by agarose filter paper diffusion method and microdilution *** use Live/Dead Baclight Bacterial Viability Kit test *** and *** treated with β-*** observe the cell membrane and inner structure by scanning electron microscope and transmission electron *** found that β-casein 65 has cell killing and injury effect by damage cell *** membrane exhibits wrinkles,holes,or even *** interaction between β-casein65 and the genomic DNA or RNA was examined by agarose gel *** was no significant difference when *** and *** treated with β-*** study confirmed the antibacterial function and possible mechanism of β-casein *** study will be carried out on animal model.β-casein65 will have a huge potential clinical value for preventing infection.

关键词： antibacterial peptide β-casein 65 peptide breast milk

β3-adrenergic-cAMP signaling regulated miRNAs in mouse and human brown adipocytes

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β3-adrenergic-cAMP signaling regulated miRNAs in mouse and ...

第十三届江浙沪儿科学术会议暨2016年浙江省医学会儿科学学术年会

作者： Lianghui You YanYan Zhang Yao Gao Xing Wang Juan Wen Chunmei Shi xianwei cui Xirong Guo Jiaqin Wang Chenbo Ji Nanjing Maternity and Child Health Care Institute Nanjing Maternity and Child Health Care Hospital Department of Child Health care Nanjing Maternal and Child Health Hospital Affiliated to Nanjing Medical University Department of Pediatrics The Third Affiliated Hospital of Xinxiang Medical University

Background:The enhanced brown adipose tissue(BAT) activity and "browning" of white adipose tissue(WAT)can promote the expenditure of excess stored energy,and reduce prevalence of obesity and associated metabolic *** is known as a potent stimulator of BAT,activating BAT primarily through the β3-adrenergic-cAMP *** have been regarded as molecular regulators involved in metabolically activated mouse BAT,however,few studies focus on miRNA expression in human brown *** aims of this study were to set up a cold-stimulated mouse BAT miRNA profile and compare differential miRNAs in mouse and human brown adipocytes in vitro upon P 3-adrenergic-cAMP signaling stimulation,further increasing our understanding on human BAT function and providing potential molecular therapeutic targets to increase excess energy and treat ***:Deep RNA-sequencing was performed to determine miRNAs levels in mouse BAT upon cold exposure for one ***-based miRNA validation was applied among mouse BAT and inguinal *** selected differentially miRNAs were characterized by gene ontology(GO) enrichment and KEGG pathway analysis,of their target ***,these miRNAs were assessed in mouse and human differentiated brown adipocytes cells in vitro treated with β 3-adrenergic receptor agonist CL316,243 and cAMP inducing agent ***:38 miRNAs upregulated and 44 miRNAs downregulated were identified in mouse BAT after cold exposure(Ifold changel ≥1,P ≤0.05).A total of 21 miRNAs were validated in mouse BAT and inguinal WAT and were predicted to target genes with functions potentially relevant to BAT activation and *** with the abnormally expressed miRNAs in BAT and WAT,miR-455-5 p expression were increased and miR-23 b-5 p,miR-133 a-3 p,miR-135-5 p,miR-491-5 p and miR-150-3 p were decreased in mouse differentiated brown adipocytes cells after treatment with CL-316,243 and *** interest,these selected miRNAs were al

关键词： Brown adipose miRNA cold stimulation CL316 243 forskolin

LncRNA GM13133 is a negative regulator in mouse white adipocytes differentiation and drives characteristic of brown adipocytes

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LncRNA GM13133 is a negative regulator in mouse white adipoc...

第十三届江浙沪儿科学术会议暨2016年浙江省医学会儿科学学术年会

作者： Lianghui You Lingxia Pang Lei Yang YanYan Zhang Juan Wen Xing Wang Chunmei Shi Xirong Guo xianwei cui Chen bo Ji Nanjing Maternity and Child Health Care Institute Nanjing Maternity and Child Health Care Hospital Department of Child Health careNanjing Maternal and Child Health Hospital

Adipose tissue contains thermogenic adipocytes(brown and beige) that oxidize nutrients at exceptionally high rates via non-shivering *** of white adipocytes has been considered as a promising strategy for treating obesity and associated metabolic ***,long noncoding RNAs play a crucial role in regulating tissue development and participating in disease pathogenesis,yet their effects on the conversion of mouse white to brown-like adipocytes and thermogenic function were not totally *** present study,we identified a mouse brown adipose specific expressed IncRNA,termed as GM13133,and showed its expression were decreased during the late stage of mouse white adipocyte *** of GM13133 didn’ t affect the proliferation of mouse white pre-adipocytes,but inhibited white adipocyte differentiation by decreasing lipid *** expression of GM13133 also significantly drove the conversion of white to brown-like adipocytes with the enhanced mitochondrial biogenesis and induced brown adipocytes specific markers ***,GM13133 was cold induced via cyclic AMP stimulation and modulated cyclic AMP-stimulated white adipocytes thermogenic *** results provide a IncRNA-mediated modulation in primary mouse white adipocyte differentiation and further indicate the functional significance of GM13133 in inducing browning of mouse white adipocytes and maintenance of thermogenesis.

关键词： GM13133 white adipocytes differentiation browning obesity

335例剖宫产切口部位子宫内膜异位症的临床分析

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重庆医学 2024年第11期53卷 1610-1615页

作者：崔艳崔县伟刘岚南京医科大学附属妇产医院妇产科南京210004 南京医科大学附属妇产医院医学研究中心南京210004

目的分析剖宫产切口部位子宫内膜异位症(CSE)的临床特征与分型情况。方法回顾性分析2016年1月至2022年6月该院收治的335例CSE患者临床资料,分析病灶最大径与其他临床指标的相关性,比较不同病变分型患者的临床特征。结果 335例患者发... 详细信息

目的分析剖宫产切口部位子宫内膜异位症(CSE)的临床特征与分型情况。方法回顾性分析2016年1月至2022年6月该院收治的335例CSE患者临床资料,分析病灶最大径与其他临床指标的相关性,比较不同病变分型患者的临床特征。结果 335例患者发病年龄为(30.2±4.2)岁,潜伏期为1个月至15年,平均(35.7±29.3)个月。328例患者接受手术治疗,48例(14.6%)患者既往剖宫产次数≥2次,既往剖宫产次数≥2次者发病年龄和病灶最大径高于1次者,差异有统计学意义(P鞘膜型>皮下型,差异有统计学意义(P<0.05);腹膜型血清糖类抗原125(CA125)水平、术中出血量、使用引流管和补片的比例均明显高于皮下型和鞘膜型(P<0.05)。病灶最大径与孕次、剖宫产次数、病程、术中出血量、病灶侵及深度及使用引流管、补片的比例呈正相关(P<0.05)。结论剖宫产次数在一定程度上影响CSE患者的病灶大小,腹膜型患者的病灶较大,术中出血量和使用补片比例较高,应引起临床重视。

关键词：子宫内膜异位症剖宫产术临床分型诊断治疗