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The m^(6)A reader YTHDC2 maintains visual function and retinal photoreceptor survival through modulating translation of PPEF2 and PDE6B

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Journal of Genetics and Genomics 2024年第2期51卷 208-221页

作者： Yeming Yang Xiaoyan Jiang Junyao Chen Lu Liu Guo Liu Kuanxiang Sun Wenjing Liu xianjun zhu Qiuyue Guan The Sichuan Provincial Key Laboratory for Human Disease Gene Study Center for Medical GeneticsSichuan Provincial People's HospitalUniversity of Electronic Science and Technology of ChinaChengduSichuan 610072China Department of Geriatrics Sichuan Provincial People's HospitalUniversity of Electronic Science and Technology of ChinaChengduSichuan 610072China Research Unit for Blindness Prevention of Chinese Academy of Medical Sciences(2019RU026) Sichuan Academy of Medical Sciences and Sichuan Provincial People's HospitalChengduSichuan 610072China Qinghai Key Laboratory of Qinghai Tibet Plateau Biological Resources Chinese Academy of Sciences and Qinghai Provincial Key Laboratory of Tibetan Medicine ResearchNorthwest Institute of Plateau BiologyXiningQinghai 810008China Henan Eye Institute Henan Eye HospitalPeople's Hospital of Zhengzhou UniversityHenan Provincial People's HospitalZhengzhouHenan 450003China

Inherited retinal dystrophies (IRDs) are major causes of visual impairment and irreversible blindness worldwide, while the precise molecular and genetic mechanisms are still elusive. N6-methyladenosine (m^(6)A) modification is the most prevalent internal modification in eukaryotic mRNA. YTH domain containing 2 (YTHDC2), an m^(6)A reader protein, has recently been identified as a key player in germline development and human cancer. However, its contribution to retinal function remains unknown. Here, we explore the role of YTHDC2 in the visual function of retinal rod photoreceptors by generating rod-specific Ythdc2 knockout mice. Results show that Ythdc2 deficiency in rods causes diminished scotopic ERG responses and progressive retinal degeneration. Multi-omics analysis further identifies Ppef2 and Pde6b as the potential targets of YTHDC2 in the retina. Specifically, via its YTH domain, YTHDC2 recognizes and binds m^(6)A-modified Ppef2 mRNA at the coding sequence and Pde6b mRNA at the 5′-UTR, resulting in enhanced translation efficiency without affecting mRNA levels. Compromised translation efficiency of Ppef2 and Pde6b after YTHDC2 depletion ultimately leads to decreased protein levels in the retina, impaired retinal function, and progressive rod death. Collectively, our finding highlights the importance of YTHDC2 in visual function and photoreceptor survival, which provides an unreported elucidation of IRD pathogenesis via epitranscriptomics.

关键词： Epitranscriptomics N^(6)-methyladenosine(m^(6)A) Inherited retinal dystrophies YTHDC2 Retina function Photoreceptor degeneration

The endoplasmic reticulum membrane protein complex subunit Emc6 is essential for rhodopsin localization and photoreceptor cell survival

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Genes & Diseases 2024年第2期11卷 1035-1049页

作者： Kuanxiang Sun Lu Liu Xiaoyan Jiang Heting Wang Lin Wang Yeming Yang Wenjing Liu Lin Zhang Xiaohui Zhao xianjun zhu Health Management Center Sichuan Provincial People's HospitalUniversity of Electronic Science and Technology of ChinaChengduSichuan 610072China Key Laboratory of Tibetan Medicine Research Chinese Academy of Sciences and Qinghai Provincial Key Laboratory of Tibetan Medicine ResearchNorthwest Institute of Plateau BiologyXiningQinghai 810008China The Sichuan Provincial Key Laboratory for Human Disease Gene Study and Department of Laboratory Medicine Center for Medical GeneticsSichuan Provincial People's HospitalUniversity of Electronic Science and Technology of ChinaChengduSichuan 610072China Research Unit for Blindness Prevention of Chinese Academy of Medical Sciences (2019RU026) Sichuan Academy of Medical Sciences and Sichuan Provincial People's HospitalChengduSichuan 610072China Department of Ophthalmology The First People's Hospital of ShangqiuShangqiuHenan 476000China

The endoplasmic reticulum(ER)membrane protein complex(EMC)is responsible for monitoring the biogenesis and synthetic quality of membrane proteins with tail-anchored or multiple transmembrane *** EMC subunit EMC6 is one of the core members of EMC and forms an enclosed hydrophilic vestibule in cooperation with *** studies demonstrating that deletion of EMC3 led to rhodopsin mislocalization in rod photoreceptors of mice,the precise mechanism leading to the failure of rhodopsin trafficking remains ***,we generated the first rod photoreceptor-specific knockout of Emc6(RKO)and cone photoreceptor-specific knockout of Emc6(CKO)mouse *** of Emc6 in rod photoreceptors led to progressive shortening of outer segments(OS),impaired visual function,mislocalization and reduced expression of rhodopsin,and increased gliosis in rod *** addition,CKO mice displayed the progressive death of cone photoreceptors and abnormal localization of cone opsin ***,proteomics analysis of the RKO mouse retina illustrated that several cilium-related proteins,particularly anoctamin-2(ANO2)and transmembrane protein 67(TMEM67),were significantly down-regulated prior to OS *** rod photoreceptor cilia and mislocalized membrane disc proteins were evident in RKO *** data revealed that in addition to monitoring the synthesis of rhodopsin-dominated membrane disc proteins,EMC6 also impacted rod photoreceptors'ciliogenesis by regulating the synthesis of membrane proteins associated with cilia,contributing to the mislocalization of membrane disc proteins.

关键词： ANO2 Cilium EMC6 ER membrane protein complex Mislocalization Photoreceptor degeneration TMEM67

老旧城区排水管道地箭式微顶管技术应用研究——以武汉市老城区改造为例

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科技和产业 2024年第8期24卷 263-269页

作者：朱先均罗利中国五冶集团有限公司成都610063

以武汉市历史风貌区老旧城区排水改造工程为背景,对复杂地质下地箭式微型顶管施工进行分析研究,介绍了微型顶管技术的工艺原理,重点阐述了微型顶管技术在城市排水改造工程中的施工工艺及控制措施,提出了高压旋喷桩止水帷幕+洞口橡胶止... 详细信息

以武汉市历史风貌区老旧城区排水改造工程为背景,对复杂地质下地箭式微型顶管施工进行分析研究,介绍了微型顶管技术的工艺原理,重点阐述了微型顶管技术在城市排水改造工程中的施工工艺及控制措施,提出了高压旋喷桩止水帷幕+洞口橡胶止水装置以及触变泥浆智能注浆系统对流沙、涌泥的防治方法,切实解决了传统开槽法排水管道施工的不足,为类似工程施工提供借鉴。

关键词：地箭式微型顶管排水改造钢沉井激光经纬仪注浆系统

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交叉融合性新专业柔性电子学的课程体系建设

高等工程教育研究 2024年第3期 15-18页

作者：朱先军刘蕾蕾郭艳东许吉南京邮电大学电子与光学工程学院、柔性电子(未来技术)学院

柔性电子学是面向国家重大战略需求的跨学科交叉融合领域设立的新专业。本文阐明了柔性电子学专业多学科交叉融合性的特点,阐述了面向交叉融合的前沿学科需求,突出多学科交叉融合的特点,进行柔性电子人才培养的课程体系构建和优化的思路... 详细信息

柔性电子学是面向国家重大战略需求的跨学科交叉融合领域设立的新专业。本文阐明了柔性电子学专业多学科交叉融合性的特点,阐述了面向交叉融合的前沿学科需求,突出多学科交叉融合的特点,进行柔性电子人才培养的课程体系构建和优化的思路,重点介绍了专业核心课程的特色和实践教学环节设置,详细介绍了传统课程融合交叉学科特色的教学内容改革。

关键词：交叉融合柔性电子人才培养课程体系

基于预连接镍链的高应变敏感度动态可调电磁干扰屏蔽弹性体

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Science China Materials 2024年第2期67卷 629-641页

作者：卞敬周兴成周翔马林峰朱先军李建民刘淑娟赵强 College of Electronic and Optical Engineering&College of Flexible Electronics(Future Technology) National and Local Joint Engineering Laboratory of RF Integration and Micro-Assembly TechnologyNanjing University of Posts and TelecommunicationsNanjing 210023China State Key Laboratory of Organic Electronics and Information Displays&Jiangsu Key Laboratory for Biosensors Institute of Advanced Materials(IAM)Nanjing University of Posts&TelecommunicationsNanjing 210023China

具有动态可调电磁屏蔽性能的电磁干扰屏蔽材料备受关注,但其目前仍然存在制备复杂、厚度大、触发方式不便、调节范围窄等缺点.我们通过将尖刺镍微粒精确地分散到聚二甲基硅氧烷基体中形成预连接的链状结构,制备出了具有可开关电磁屏蔽... 详细信息

具有动态可调电磁屏蔽性能的电磁干扰屏蔽材料备受关注,但其目前仍然存在制备复杂、厚度大、触发方式不便、调节范围窄等缺点.我们通过将尖刺镍微粒精确地分散到聚二甲基硅氧烷基体中形成预连接的链状结构,制备出了具有可开关电磁屏蔽性能的弹性体材料.弹性体在机械拉伸和释放过程中表现出连续和可逆的电磁干扰屏蔽性能.原始弹性体具有良好的阻抗匹配和低介电损耗,允许大部分电磁波通过.拉伸使预连接的短链彼此接触,在内部形成大量的微尺度导电网络,显著增强了电导损耗能力,实现了较强的电磁干扰屏蔽能力.此外,我们通过无线应变传感系统验证了智能电磁干扰屏蔽弹性体的应用潜力,证明了可穿戴智能电磁干扰屏蔽精确监测人体运动的可能性.

关键词： adjustable electromagnetic interference shielding conductive elastomer electromagnetic sensing negative piezoresistivity

第二十七届当代中国建筑创作论坛·“成长”分论坛:共赴跨越30年的回溯与心韵之旅

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世界建筑导报 2024年第2期39卷 51-53页

作者：赵慧仪(整理) 王子睿(整理) 朱显珺(整理) 武汉大学城市设计学院东南大学建筑学院深圳大学建筑与城市规划学院

1.“成长”分论坛·缘起1990年,深圳大学主办的第五届建筑创作论坛,许安之院长首次成立了学生组委会,来自11所建筑高校的20多位优秀学生代表,如李兴钢、张利、张彤、宋照清、毛刚,祝晓峰、张之杨等,受邀于深圳这座前沿之城相聚,进行评... 详细信息

1.“成长”分论坛·缘起1990年,深圳大学主办的第五届建筑创作论坛,许安之院长首次成立了学生组委会,来自11所建筑高校的20多位优秀学生代表,如李兴钢、张利、张彤、宋照清、毛刚,祝晓峰、张之杨等,受邀于深圳这座前沿之城相聚,进行评图交流和参观考察,拓展建筑视野,结缘深厚友谊。30余载光阴,曾经的学子在不同领域、城市蓬勃发展,涌现为建筑大师、资深教育家和实践者,持续启迪新生代。这次青年学生代表的交流活动,也因此成为了当代中国建筑创作论坛的首创和唯一,影响深远。

关键词：建筑大师优秀学生建筑创作深圳大学深厚友谊参观考察

Loss of Wtap results in cerebellar ataxia and degeneration of Purkinje cells

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Journal of Genetics and Genomics 2022年第9期49卷 847-858页

作者： Yeming Yang Guo Huang Xiaoyan Jiang Xiao Li Kuanxiang Sun Yi Shi Zhenglin Yang xianjun zhu The Sichuan Provincial Key Laboratory for Human Disease Gene Study and Department of Laboratory Medicine Center for Medical GeneticsSichuan Provincial People’s HospitalUniversity of Electronic Science and Technology of ChinaChengduSichuan 610072China Research Unit for Blindness Prevention of Chinese Academy of Medical Sciences(2019RU026) Sichuan Academy of Medical Sciences and Sichuan Provincial People’s HospitalChengduSichuan 610072China Key Laboratory of Tibetan Medicine Research Chinese Academy of Sciences and Qinghai Provincial Key Laboratory of Tibetan Medicine ResearchNorthwest Institute of Plateau BiologyXiningQinghai 810008China

N^(6)-methyladenosine(m^(6)A)modification,which is achieved by the METTL3/METTL14/WTAP methyltransferase complex,is the most abundant internal mRNA *** recent evidence indicates that m^(6)A can regulate neurodevelopment as well as synaptic function,the roles of m^(6)A modification in the cerebellum and related synaptic connections are not well ***,we report that Purkinje cell(PC)-specific WTAP knockout mice display early-onset ataxia concomitant with cerebellar atrophy due to extensive PC degeneration and apoptotic cell *** of Wtap also causes the aberrant degradation of multiple PC *** depletion leads to decreased expression levels of METTL3/14 and reduced m^(6)A methylation in ***,the expression of GFAP and NF-L in the degenerating cerebellum is increased,suggesting severe neuronal *** conclusion,this study demonstrates the critical role of WTAP-mediated m^(6)A modification in cerebellar PCs,thus providing unique insights related to neurodegenerative disorders.

关键词： N^(6)-methyladenosine Wtap METTL3 METTL14 Purkinje cell Ataxia Cerebellum

ERRATUM TO:Cholesterol homeostasis regulated by ABCA1 is critical for retinal ganglion cell survival

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Science China(Life Sciences) 2024年第7期67卷 1548-1548页

作者： Jialiang Yang Yuhong Chen Tongdan Zou Bai Xue Fang Yang Xiangzhou Wang Yibo Huo Boyun Yan Yuxia Xu Shiyu He Yi Yin Jing Wang Xiong zhu Lin Zhang Yu Zhou Zhengfu Tai Ping Shuai Man Yu Qian Luo Yilian Cheng Bo Gong xianjun zhu Jing Zhang Xinghuai Sun Ying Lin Houbin Zhang Zhenglin Yang Key Laboratory for Human Disease Gene Study of Sichuan Province and Institute of Laboratory Medicine Sichuan Provincial People’s HospitalUniversity of Electronic Science and Technology of ChinaChengdu 610072China Department of Ophthalmology and Visual Science Eye and Ear Nose Throat(ENT)HospitalFudan UniversityShanghai 200032China School of Optoelectronic Science and Engineering University of Electronic Science and Technology of ChinaChengdu 611731China Institute of Chengdu Biology Sichuan Translational Medicine HospitalChinese Academy of SciencesChengdu 610093China Department of Ophthalmology Sichuan Provincial People’s HospitalUniversity of Electronic Science and Technology of ChinaChengdu 610072China Research Unit for Blindness Prevention of Chinese Academy of Medical Sciences Sichuan Academy of Medical Sciences&Sichuan Provincial People’s HospitalChengdu 610072China

Erratum to:SCIENCE CHINA Life Sciences,Volume 66,Issue 2:211-225(2022),https://***/10.1007/s11427-021-2126-2 This paper contains an error in Figure 4C,where the representative images of filipin staining of retinal sections from 6-month-old mice were *** provide the correct picture for Figure 4C as *** statistical result in Figure 4D was corrected as *** new Figure 4 does not affect the conclusion of this article.

关键词： Figure critical conclusion

ERRATUM TO: Cholesterol homeostasis regulated by ABCA1 is critical for retinal ganglion cell survival

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Science China Life Sciences 2024年

作者： Jialiang Yang Yuhong Chen Tongdan Zou Bai Xue Fang Yang Xiangzhou Wang Yibo Huo Boyun Yan Yuxia Xu Shiyu He Yi Yin Jing Wang Xiong zhu Lin Zhang Yu Zhou Zhengfu Tai Ping Shuai Man Yu Qian Luo Yilian Cheng Bo Gong xianjun zhu Jing Zhang Xinghuai Sun Ying Lin Houbin Zhang Zhenglin Yang Key Laboratory for Human Disease Gene Study of Sichuan Province and Institute of Laboratory Medicine Sichuan Provincial People's Hospital University of Electronic Science and Technology of China Department of Ophthalmology Sichuan Provincial People's Hospital University of Electronic Science and Technology of China Department of Ophthalmology and Visual Science Eye and Ear Nose Throat (ENT) Hospital Fudan University Institute of Chengdu Biology Sichuan Translational Medicine Hospital Chinese Academy of Sciences School of Optoelectronic Science and Engineering University of Electronic Science and Technology of China Research Unit for Blindness Prevention of Chinese Academy of Medical Sciences Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital

Erratum to:SCIENCE CHINA Life Sciences, Volume 66, Issue 2:211-225 (2022), https://***/10.1007/s11427-021-2126-2This paper contains an error in Figure 4C, where the representative images of filipin staining of retinal sections from 6-month-old mice were *** provide the correct picture for Figure 4C as follows. The statistical result in Figure 4D was corrected as well. This new Figure 4does not affect the conclusion of this article.

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