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Gastrodin Attenuates Dextran Sulfate Sodium-Induced Colitis in Mice via Modulating Gut Microbiota and Inhibiting NF- κB Signaling Pathway

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Food Science and Human Wellness 2024年

作者： Xinyi Gao xianjun liu Xianglong Meng Mingchuan Wang Mengyuan Wang Boyao Sun Chengxuan Jia Jintian Gao Chengyi Xu Hao Li Yang Jiang Department of Gastrointestinal Colorectal and Anal Surgery The China-Japan Union Hospital of Jilin University College of Biological and Food Engineering Jilin Engineering Normal University Department of Burns Surgery The First Hospital of Jilin University Medical Faculty Hetao College Deartment of Nephrology The Second Hospital of Jilin University

Gastrodin (GAS)， the principal bioactive composition of Gastrodia elata Blume， has potential for pharmaceutical applications. Several studies in recent years have shown that GAS may enhance neurotrophic benefits， reduce inflammation， and act as an antioxidant. In this study， we sought to identify the molecular mechanisms underlying the protective benefits of GAS against colitis induced by dextran sodium sulfate (DSS) in mice. GAS (200 mg/kg) significantly ameliorated the severity of colitis in mice caused by DSS， as evidenced by an increase in colon length， a reduction in disease activity index， a decrease in tissue damage， and a reduction in body weight loss. Additionally， GAS inhibited DSS-induced hyperactivation of inflammation-related NF-κB signaling pathways to reduce the production of inflammatory mediators， thereby mitigating the inflammatory response in mice. Furthermore， the administration of GAS restored the function of the gastrointestinal barrier by increasing the count of goblet cells， as well as the levels of tight junction-associated proteins， including ZO-1， Occludin， and Claudin-3. GAS also influenced the overall richness of the gut microbiota， as shown by 16S rRNA sequencing analysis， consequently boosting the proliferative rate of probiotic species， such as Lachnospiraceae and Muribaculaceae， while reducing the richness of harmful bacteria including Escherichia＿Shigella， Enterobacteriaceae， Bacteroidaceae， and Bacteroides. GAS (200 mg/kg) alleviated ulcerative colitis (UC) by modulating gut dysbiosis， as demonstrated by a fecal microbial transplantation (FMT) test. Furthermore， inflammatory damage induced by lipopolysaccharide (LPS) was averted in RAW264.7 cells by GAS administration， hence preventing the NF-κB signaling pathway from being activated in these experimental conditions conducted in vitro. Overall， the data indicate that GAS treatment effectively reduces Colitis caused by DSS by regulating gut microbiota， suppressing inflammation， and preserving the mucosal barrier integrity.

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Cell cycle dysregulation with overexpression of KIF2C/MCAK is a critical event in

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Genes & Diseases 2023年第1期10卷 212-227页

作者： Xiaofeng Zuo Peixin Meng Yuxin Bao Chuntao Tao Yitao Wang xianjun liu Youquan Bu Jiang Zhu Department of Otolaryngology The First Affiliated Hospital of Chongqing Medical UniversityChongqing Medical UniversityChongqing 400016China Department of Biochemistry and Molecular Biology Chongqing Medical UniversityChongqing 400016China Molecular Medicine and Cancer Research Center Chongqing Medical UniversityChongqing 400016China

Nasopharyngeal carcinoma(NPC)is a common malignant carcinoma of the head and neck,and the biological mechanisms underlying the pathogenesis of NPC remain not fully understood.In the present study,we systematically analyzed four independent NPC transcriptomic datasets and focused on identifying the critical molecular networks and novel key hub genes implicated in NPC.We found totally 170 common overlapping differentially expressed genes(DEGs)in the four NPC datasets.GO and KEGG pathway analysis revealed that cell cycle dysregulation is a critical event in NPC.Protein-protein interaction(PPl)network analysis identified a 15 hub-gene corenetwork with overexpressedkinesin family member2C(KIF2C)as a central regulator.Loss-of-function study demonstrated that knockdown of KiF2C significantly inhibited cell growth and cell motility,and delayed cell cycle progression,accompanied with dramatic mitotic defects in spindle formation in NPC cells.RNA-seq analysis revealed that KIF2c knockdown led to deregulation of various downstream genes.KiF2C could also regulate the AKT/mTOR pathways,and enhance paclitaxel sensitivity in NPC cells.Taken together,our results suggest that cell cycle dysregulation is a critical event during NPC pathogenesis and KIF2C is a novel key mitotic hub gene with therapeutic potential in NPC.

关键词： Bioinformatics Cell cycle KIF2C Mitosis Nasopharyngeal carcinoma

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智慧教育背景下高等教育管理变革探究

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高校教育管理 2024年第1期18卷 24-32页

作者：刘献君华中科技大学教育科学研究院湖北武汉430074

教育随着时代的发展、技术的进步而变革和发展。随着数字化技术的发展,教育开始进入智慧教育阶段。智慧教育包括相互融通的学习场景、灵活多元的学习方式和富有弹性的组织管理,是数字化时代教育的新形态。若要有效推进智慧教育,必须实... 详细信息

教育随着时代的发展、技术的进步而变革和发展。随着数字化技术的发展,教育开始进入智慧教育阶段。智慧教育包括相互融通的学习场景、灵活多元的学习方式和富有弹性的组织管理,是数字化时代教育的新形态。若要有效推进智慧教育,必须实现高等教育管理的转型变革。智慧教育背景下,高等教育管理要创新教育管理观念,重视数字思维、适需服务和人机协同;拓展教育管理内涵,重视数据治理、环境再造和制度设计;完善教育管理的组织结构,不仅要创建矩阵型组织结构,还要将项目组作为新的治理单元,并建立数字化管理机构。

关键词：智慧教育高等教育管理教育数字化数字化思维数据治理

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Research on flow stress in ferrite deformation of a Ti-IF steel

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Journal of University of Science and Technology Beijing 2006年第1期13卷 34-36页

作者： Guang Xu Chushao Xu Jiarong Zhao xianjun liu College of Material Science and Engineering Chongqing University 400044 Chongqing China College of Materials and Metallurgy Wuhan University of Science and Technology 430081 Wuhan China

The experiments of the ferrite warm deformation of ultra-low carbon （ULC） Ti-IF steel were carded out on a hot simulator and the influences of deformation temperature, strain, and strain rate on the flow stress were analyzed. New flow stress models suitable to ferrite warm forming of Ti-IF steel were given on the basis of analyzing the influence of deformation technology parameters on the flow stress.

关键词： ULC Ti-IF steel ferrite deformation flow stress model

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制革中悬挂式转鼓作用特征

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皮革科学与工程 2024年第1期34卷 47-52,60页

作者：刘贤军单志华明新旭腾新材料有限公司浙江嘉兴314000 四川大学轻工科学与工程学院四川成都610065

皮革机械是皮革工业进步的重要支撑。转鼓的发明是制革湿操作迈入节水高效的重要标志。由此,转鼓也成为完成制革化学过程的最关键设备。由于圆形悬挂式转鼓具有固定的运行轨迹,皮/坯革不同的加工效果需要通过调整转鼓内部的结构及操作... 详细信息

皮革机械是皮革工业进步的重要支撑。转鼓的发明是制革湿操作迈入节水高效的重要标志。由此,转鼓也成为完成制革化学过程的最关键设备。由于圆形悬挂式转鼓具有固定的运行轨迹,皮/坯革不同的加工效果需要通过调整转鼓内部的结构及操作参数。文章探索性分析了转鼓对皮/坯革的共性作用特征,描述了制革工序中转鼓内构造与操作参数之间的一些关联,包括由此形成的揉搓、挤压、拉伸等机械作用对皮/坯革内物质溶出与外加材料渗入的影响。

关键词：皮革机械转鼓结构机械作用制革工序

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Activities of autonomic neurotransmitters in meibomian gland tissues are associated with menopausal dry eye

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Neural Regeneration Research 2012年第35期7卷 2761-2769页

作者： Lianxiang Li Dongling Jin Jinsheng Gao Liguang Wang xianjun liu Jingzhang Wang Zhongxin Xu Department of Anatomy Histology and Embryology Laboratory of Basic Medicine Medical College Hebei University of Engineering Department of Pathology Medical College Hebei University of Engineering Department of Physiology Medical College Hebei University of Engineering Department of Biochemistry Medical College Hebei University of Engineering

The secretory activities of meibomian glands are regulated by the autonomic nervous system, The change in density and activity of autonomic nerves in meibomian glands during menopause play an important role in the pathogenesis of dry eye. In view of this, we established a dry eye rat model by removing the bilateral ovaries. We used neuropeptide Y and vasoactive intestinal polypeptide as markers of autonomic neurotransmitters. Our results showed that the concentration of estradiol in serum significantly decreased, the density of neuropeptide Y immunoreactivity in nerve fibers significantly increased, the density of vasoactive intestinal polypeptide immunoreactivity in nerve fibers significantly decreased, and the ratio of vasoactive intestinal polypeptide/neuropeptide Y positive staining significantly decreased. These results suggest that a decrease in ovary activity may lead to autonomic nervous system dysfunction, thereby affecting the secretory activity of the meibomian gland, which participates in sexual hormone imbalance-induced dry eye.

关键词： ovariectomy meibomian gland meibomian gland dysfunction neuropeptide Y vasoactive intestinalpolypeptide autonomic nervous system dysfunction dry eye sexual hormone rats autonomicnerve neural regeneration

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构建全球最精准的千猪单倍型库和猪下呼吸道微生物抗药基因(ARGs)图谱

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中国科学：生命科学 2024年第2期54卷 367-368页

作者：刘显军陈启军沈阳农业大学动物科学与医学学院沈阳110866

猪肉是人类主要的食品成分.随着世界人口的不断增加,世界各国对猪肉的消费逐渐增加.近年来,我国生猪年均出栏量7亿头左右,猪肉的生产量和消费量均占世界一半左右. 2022全年我国生猪出栏69995万头,猪肉产量5541万吨,猪肉产量占猪牛羊禽... 详细信息

猪肉是人类主要的食品成分.随着世界人口的不断增加,世界各国对猪肉的消费逐渐增加.近年来,我国生猪年均出栏量7亿头左右,猪肉的生产量和消费量均占世界一半左右. 2022全年我国生猪出栏69995万头,猪肉产量5541万吨,猪肉产量占猪牛羊禽肉产量的60%.猪肉已是我国人民的生活必需品,也是畜牧业生产和经济的主体[1].因此,在我国养猪业具有“无猪不稳,猪粮安天下”的战略地位.

关键词：猪肉产量畜牧业生产生活必需品禽肉产量食品成分下呼吸道养猪业出栏量

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Glycosylation of dentin matrix protein 1 is a novel key element for astrocyte maturation and BBB integrity

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Protein & Cell 2018年第3期9卷 298-309页

作者： Bo Jing Chunxue Zhang xianjun liu Liqiang Zhou Jiping liu Yinan Yao Juehua Yu Yuteng Weng Min Pan Jie liu Zuolin Wang Yao Sun Yi Eve Sun Tongji University School of Medicine Stem Cell Translational Research Center Tongji Hospital Shanghai 200065 China Department of Oral Implantology School of Stomatology Tongji University Shanghai Engineering Research Center of Tooth Restoration and Regeneration Shanghai 200072 China Department of Psychiatry and Biobehavioral Sciences David Geffen School of Medicine University of California Los Angeles CA 90095 USA Collaborative Innovation Center for Brain Science Tongji University Shanghai 200092 China

The blood-brain barrier （BBB） is a tight boundary formed between endothelial cells and astrocytes, which separates and protects brain from most pathogens as well as neural toxins in circulation. However, detailed molecular players involved in formation of BBB are not completely known. Dentin matrix protein I （DMP1）-proteoglycan （PG）, which is known to be involved in mineralization of bones and dentin, is also expressed in soft tissues including brain with unknown functions. In the present study, we reported that DMPI-PG was expressed in brain astrocytes and enriched in BBB units. The only glycosylation site of DMP1 is serine89 （S89） in the N-terminal domain of the protein in mouse. Mutant mice with DMP1 point mutations changing S89 to glycine （S89G）, which completely eradicated glycosylation of the protein, demonstrated severe BBB disruption. Another breed of DMP1 mutant mice, which lacked the C-terminal domain of DMP1, manifested normal BBB function. The polarity of S89G-DMP1 astrocytes was disrupted and cell-cell adhesion was loosened. Through a battery of analyses, we found that DMP1 glycosylation was critically required for astrocyte maturation both in vitro and in vivo. S89G-DMP1 mutant astrocytes failed to express aquaporin 4 and had reduced laminin and ZO1 expression, which resulted in disruption of BBB. Interestingly, overexpression of wild-type DMP1-PG in mouse brain driven by the nestin promoter elevated laminin and ZO1 expression beyond wild type levels and could effectively resisted intravenous mannitol-induced BBB reversible opening. Taken together, our study not only revealed a novel element, i.e., DMP1-PG, that reg- ulated BBB formation, but also assigned a new function to DMP1-PG.

关键词： blood-brain barrier dentin matrix protein1 glycosylation astrocyte cell adhesion proteoglycan

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裸藻β-1,3-葡聚糖对断奶仔猪生长性能、血清炎症细胞因子含量及肠道菌群结构的影响

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动物营养学报 2024年第3期36卷 1588-1598页

作者：杨馥嘉陈静刘显军李海东葛冬冬曲盛卿刘景军沈阳农业大学动物科学与医学学院沈阳110866 沈阳博睿农牧科技有限公司沈阳110300 山东纳美达生物科技有限公司济南250100

本试验旨在研究裸藻β-1,3-葡聚糖对仔猪生长性能、血清炎症细胞因子含量及肠道菌群结构的影响,为裸藻β-1,3-葡聚糖的开发和应用提供依据。选取150头40日龄左右、体重[(13.06±0.26)kg]相近的“杜洛克×长白×大白猪”三元杂断奶仔猪,... 详细信息

本试验旨在研究裸藻β-1,3-葡聚糖对仔猪生长性能、血清炎症细胞因子含量及肠道菌群结构的影响,为裸藻β-1,3-葡聚糖的开发和应用提供依据。选取150头40日龄左右、体重[(13.06±0.26)kg]相近的“杜洛克×长白×大白猪”三元杂断奶仔猪,随机分为5个组,每组5个重复,每个重复6头猪。对照组饲喂基础饲粮,试验组分别在基础饲粮中添加50、100、150 mg/kg的裸藻β-1,3-葡聚糖及100 mg/kg的酵母β-1,3-葡聚糖。预试期3 d,正试期28 d。结果表明:1)与对照组相比,饲粮中添加100 mg/kg的裸藻β-1,3-葡聚糖显著提高了断奶仔猪平均日采食量和平均日增重(P<0.05),显著降低了料重比(P<0.05)。2)与对照组相比,饲粮中添加50、100、150 mg/kg的裸藻β-1,3-葡聚糖及100 mg/kg的酵母β-1,3-葡聚糖显著提高了血清白细胞介素-1、白细胞介素-6和肿瘤坏死因子-α含量(P<0.05)。3)与对照组相比,饲粮中添加50 mg/kg的裸藻β-1,3-葡聚糖显著提高了软壁菌门(Tenericutes)相对丰度(P<0.05),饲粮中添加50和100 mg/kg的裸藻β-1,3-葡聚糖显著提高了乳杆菌科(Lactoba⁃cillaceae)相对丰度(P<0.05),饲粮中添加50和150 mg/kg的裸藻β-1,3-葡聚糖显著降低了瘤胃球菌属(Ruminococcus)相对丰度(P<0.05)。4)与对照组相比,饲粮中添加100 mg/kg的裸藻β-1,3-葡聚糖显著提高了Chao1指数(P<0.05),饲粮中添加150 mg/kg的裸藻β-1,3-葡聚糖显著降低了Simpson指数(P<0.05)。Beta多样性分析发现,饲粮中添加100 mg/kg裸藻β-1,3-葡聚糖组与对照组物种相似性最高。由此可见,饲粮中添加适量裸藻β-1,3-葡聚糖能提高断奶仔猪生长性能,降低炎症反应,改善肠道菌群多样性和结构。本试验条件下,饲粮中添加100 mg/kg裸藻β-1,3-葡聚糖效果最好。

关键词：裸藻β-1,3-葡聚糖断奶仔猪生长性能炎症细胞因子肠道菌群结构

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陈皮体外预处理对柑橘黄酮含量和感官指标的影响

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饲料工业 2024年第1期45卷 49-54页

作者：葛冬冬陈静李海东刘显军杨馥嘉沈阳农业大学动物科学与医学学院辽宁沈阳110866 沈阳博睿农牧科技有限公司辽宁沈阳110300

研究旨在探究菌发酵、酶解、菌酶协同3种体外预处理方法对陈皮营养价值的影响。试验利用发酵菌和消化酶对陈皮分别进行发酵、酶解、菌酶协同预处理,以柑橘黄酮含量和感官指标为比较标准选择最优的预处理方法和条件。结果表明:菌发酵和... 详细信息

研究旨在探究菌发酵、酶解、菌酶协同3种体外预处理方法对陈皮营养价值的影响。试验利用发酵菌和消化酶对陈皮分别进行发酵、酶解、菌酶协同预处理,以柑橘黄酮含量和感官指标为比较标准选择最优的预处理方法和条件。结果表明:菌发酵和菌酶协同处理陈皮方法显著优于酶解处理方法。其中菌发酵处理组料液比10∶7时黄酮含量显著提高(P0.05),且都优于接种量7%(P0.05)。酶解组的最佳条件为加酶量0.5%、料液比10∶7;菌酶协同处理组菌接种量间差异不显著(P>0.05);料液比10∶7和10∶8之间差异不显著(P>0.05),且都优于料液比10∶6(P<0.05);酶添加量为0.5%时,陈皮中的柑橘黄酮含量显著高于其他添加量(P<0.05)。说明菌发酵、酶解、菌酶协同3种处理方法均能提高陈皮中的柑橘黄酮含量,其中最优工艺为菌酶协同法,加菌量5%、加酶量0.5%、料液比10∶7。

关键词：陈皮发酵酶解菌酶协同感官指标

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