检索结果-南通市图书馆

Chinese Chemical Letters 2022年第6期33卷 3043-3048页

作者： Lida Ren Zhihao Ming Wei Zhang Yangwei Liao Xiaofeng Tang Bei Yan Huimin Lv xianjin Xiao Centre of Reproductive Medicine/Family Planning Research Institute Tongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China Department of Obstetrics and Gynecology Third Hospital of Shanxi Medical University.Shanxi Bethune HospitalShanxi Academy of Medical SciencesTongji Shanxi HospitalTongji Medical College.Huazhong University of Science and TechnologyTaiyuan 030032China

Enzyme assisted DNA probes are powerful tools in molecular diagnostics for their simplicity,rapidity,and low detection ***,cost of probes,difficulty in optimization and disturbance of secondary structure hindered the wider application of enzyme assisted DNA *** solve the problems,we designed a new system named sh.red-probe *** introducing two unlabeled single stranded DNA named sh. and sh. as the bridge between probe and the substrate,the same sequence of dually labeled probe with stable performance was sh.red for different mutations,thus sparing the expense and time cost on designing,synthesizing and optimizing corresponding ***,the hybridization between sh. and the substrate could overcome secondary structures,which guaranteed the detection of different *** performance and generality of the design were tested by low abundance detection in synthetic single DNA samples and the limit of detection was 0.05%for PTENR130 Q,EGFR-L858 R and 0.02%for *** genomic DNA samples,the limit of detection of 0.1%can be achieved for EGFR-L858 R,demonstrating the potential of clinical application in our design.

关键词： Nucleic acid probe EndonucleaseⅣ Secondary structure Genomic mutation SNP detection

应激性血糖升高比值联合糖化血红蛋白水平对老年急性缺血性脑卒中病人静脉溶栓后出血转化的预测价值

在线全文

学校读者我要写书评

暂无评论

实用老年医学 2024年第1期38卷 54-58页

作者：吴旭明柯先金孙波孙振杰江苏大学附属医院神经内科江苏省镇江市212001 南京医科大学附属淮安第一医院神经内科江苏省淮安市223300

目的探讨应激性血糖升高比值(sh.)联合HbA1c对老年急性缺血性脑卒中(AIS)病人静脉溶栓后出血转化(HT)的预测价值。方法纳入2018年6月至2020年12月连云港市第一人民医院和江苏大学附属医院接受重组组织型纤溶酶原激活剂(rt-PA)静脉溶栓... 详细信息

目的探讨应激性血糖升高比值(sh.)联合HbA1c对老年急性缺血性脑卒中(AIS)病人静脉溶栓后出血转化(HT)的预测价值。方法纳入2018年6月至2020年12月连云港市第一人民医院和江苏大学附属医院接受重组组织型纤溶酶原激活剂(rt-PA)静脉溶栓AIS老年住院病人共234例,依据急性期内复查CT或MRI结果,将病人分为HT组(n=50)和非HT组(n=184)。收集并比较2组病人临床基线资料。采用多因素Logistic回归分析老年AIS病人rt-PA静脉溶栓后HT的危险因素,应用ROC曲线分析sh.、HbA1c及二者联合预测老年AIS病人rt-PA后HT的价值。结果 2组年龄、溶栓前NIHSS评分、FPG、HbA1c水平,房颤、TOAST分型和梗死大面积比例差异均有统计学意义(Psh.(OR=39.443,95%CI:1.847~842.343)、HbA1c(OR=1.777,95%CI:1.091~2.859)以及大面积梗死(OR=3.093,95%CI:1.359~7.036)是老年AIS病人rt-PA静脉溶栓后HT的独立危险因素。HbA1c、sh.及二者联合预测老年AIS病人rt-PA静脉溶栓后HT风险的AUC分别为:0.631(95%CI:0.541~0.721)、0.656(95%CI:0.654~0.748)、0.741(95%CI:0.665~0.816)。HbA1c联合sh.预测的AUC显著大于两指标单独预测,差异有统计学意义(P均sh.和HbA1c是老年AIS病人rt-PA静脉溶栓后HT的独立危险因素,两者联合可更好地预测老年AIS病人rt-PA静脉溶栓后HT风险。

关键词：急性缺血性脑卒中出血转化应激性血糖升高比值糖化血红蛋白危险因素

Study on the Recent Tectonic Stress Field in the “Huosh.n Seismic Window” Region

在线全文

学校读者我要写书评

暂无评论

Earthquake Research in China 2013年第4期27卷 467-478页

作者： Ni Hongyu Liu Zemin Hong Dequan Li Lingli Zheng xianjin Xu Xin Earthquake Administration of Anhui Province

The focal mechanisms of 62 moderate-small earthquakes since 1980 in the ＂ Huosh.n seismic window＂ region are calculated with the method developed recently by Snoke, combining the use of the first motion of P, SV and sh.waves with their amplitude ratios. Based on these abundant focal mechanisms, the mean tectonic stress field in the ＂Huosh.n seismic window＂ region is inverted with the average stress tensor method, and the result sh.ws that the ＂Huosh.n seismic window＂ region is horizontally compressed in the near EW direction and horizontally dilated in the near NS direction, which is in accord with statistical results of focal mechanism parameters. We estimate the difference （also referred to as consistency parameter 0） between the force axis direction of the focal mechanism solution and the mean stress tensor, then further analyze the variation characteristics of 0 versus time, and the relationsh.p with moderately strong earthquakes in the east China region. The result indicates that 0 in the ＂ Huosh.n seismic window＂ region is in good correspondence with moderately strong earthquakes in the East China region. When 0 is lower than the mean value, corresponding moderately strong earthquakes may occur in the East China region.

关键词： Focal mechanism solutions Consistency parameter Tectonic stress field The"Huosh.n seismic window" East China region

COVID-19相关脑炎的致病机制研究进展

在线全文

学校读者我要写书评

暂无评论

中华神经医学杂志 2023年第9期22卷 953-959页

作者：柯先金孙振杰孙波江苏大学附属医院神经内科镇江212001 南京医科大学附属淮安第一医院神经内科淮安223300

严重急性呼吸综合征冠状病毒2(SARS-CoV-2)所引起的2019冠状病毒病(COVID-19)除了累及呼吸系统外,还可导致神经系统损伤,甚至出现严重的脑炎。随着感染人数越来越多,COVID-19相关脑炎的病例数也逐渐增多,但SARS-CoV-2感染引发的相关脑... 详细信息

严重急性呼吸综合征冠状病毒2(SARS-CoV-2)所引起的2019冠状病毒病(COVID-19)除了累及呼吸系统外,还可导致神经系统损伤,甚至出现严重的脑炎。随着感染人数越来越多,COVID-19相关脑炎的病例数也逐渐增多,但SARS-CoV-2感染引发的相关脑炎的机制目前仍未阐明。本文综述了COVID-19相关脑炎的可能发病机制和现有案例报道的经验性治疗,以期为今后的研究及临床治疗提供理论依据。

关键词： 2019冠状病毒病脑炎免疫反应发病机制

在线全文

学校读者我要写书评

暂无评论

“霍山窗”地区现代构造应力场研究

中国地震 2012年第3期28卷 294-303页

作者：倪红玉刘泽民洪德全李玲利郑先进徐鑫安徽省地震局合肥市长江西路558号230031 蒙城地球物理国家野外科学观测研究站安徽蒙城233500

应用Snoke于2009年提出的利用P、SV和sh.的初动和振幅比联合计算震源机制解的方法,得到"霍山窗"地区1980年以来62次中小地震的震源机制解。基于大量的震源机制解资料,根据应力张量平均法反演得到"霍山窗"地区的平均构造应力场;结果表明... 详细信息

应用Snoke于2009年提出的利用P、SV和sh.的初动和振幅比联合计算震源机制解的方法,得到"霍山窗"地区1980年以来62次中小地震的震源机制解。基于大量的震源机制解资料,根据应力张量平均法反演得到"霍山窗"地区的平均构造应力场;结果表明,其与震源机制参数统计结果一致,表现为近EW向的水平挤压和近NS向的水平拉张作用。计算单个震源机制解力轴与平均应力场主应力轴的差异,即震源机制的一致性参数θ,进而分析θ随时间变化特征及其与华东地区中强震的关系;结果表明,"霍山窗"地区震源机制的一致性参数θ对华东地区相应中强震似有一定的指示意义。

关键词：震源机制解一致性参数构造应力场 “霍山窗” 华东地区