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The Application of Bilayer Artificial Dermis Combined with VSD Technology in Chronic Wounds

Journal of Biosciences and Medicines 2024年第3期12卷 238-244页

作者： xianjin dong Huasong Luo Traumatology Orthopedics The First Affiliated Hospital of Yangtze University Jingzhou China

Background: Bilayer artificial dermis promotes wound healing and offers a treatment option for chronic wounds. Aim: Examine the clinical efficacy of bilayer artificial dermis combined with Vacuum Sealing Drainage (VSD) technology in the treatment of chronic wounds. Method: From June 2021 to December 2023, our hospital treated 24 patients with chronic skin tissue wounds on their limbs using a novel tissue engineering product, the bilayer artificial dermis, in combination with VSD technology to repair the wounds. The bilayer artificial dermis protects subcutaneous tissue, blood vessels, nerves, muscles, and tendons, and also promotes the growth of granulation tissue and blood vessels to aid in wound healing when used in conjunction with VSD technology for wound dressing changes in chronic wounds. Results: In this study, 24 cases of chronic wounds with exposed bone or tendon larger than 1.0 cm2 were treated with a bilayer artificial skin combined with VSD dressing after wound debridement. The wounds were not suitable for immediate skin grafting. At 2 - 3 weeks post-treatment, good granulation tissue growth was observed. Subsequent procedures included thick skin grafting or wound dressing changes until complete wound healing. Patients were followed up on average for 3 months (range: 1 - 12 months) post-surgery. Comparative analysis of the appearance, function, skin color, elasticity, and sensation of the healed chronic wounds revealed superior outcomes compared to traditional skin fl repairs, resulting in significantly higher satisfaction levels among patients and their families. Conclusion: The application of bilayer artificial dermis combined with VSD technology for the repair of chronic wounds proves to be a viable method, yielding satisfactory therapeutic effects compared to traditional skin flap procedures.

关键词： Bilayer Artificial Dermis Vacuum Sealing Drainage (VSD) Chronic Wounds Wound Healing Application

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腹腔镜肝切除术与开腹肝切除术治疗肝内胆管细胞癌患者短期临床疗效研究

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实用肝脏病杂志 2023年第1期26卷 120-123页

作者：东贤进苏伟李连生谢智高军林青海红十字医院肝胆胰疝外科西宁市810000 青海大学附属医院肝胆外科

目的研究腹腔镜肝切除术(LH)与开腹肝切除术(OH)治疗肝内胆管细胞癌(ICC)患者的短期临床疗效.方法2018年2月~2021年2月我院诊治的122例ICC患者,被随机分为对照组61例和观察组61例,分别接受OH或LH治疗,随访观察半年.采用ELISA法检测血清... 详细信息

目的研究腹腔镜肝切除术(LH)与开腹肝切除术(OH)治疗肝内胆管细胞癌(ICC)患者的短期临床疗效.方法2018年2月~2021年2月我院诊治的122例ICC患者,被随机分为对照组61例和观察组61例,分别接受OH或LH治疗,随访观察半年.采用ELISA法检测血清C反应蛋白(CRP)、皮质醇(Cor)和白细胞介素-6(IL-6).结果LH组手术时长、术中失血量、肛门首次排气和术后住院日分别为(232.2±50.4)min、(592.3±164.7)ml、(2.1±0.8)d和(6.5±1.3)d,显著短于或少于0H组[分别为(321.1±69.7)min、(995.5±321.4)ml、(2.7±0.7)d和(8.2±1.7)d,P0.05).结论采用LH治疗ICC患者可获得较好的手术效果,术后恢复快,可能与减轻了应急反应程度有关.

关键词：肝内胆管细胞癌腹腔镜肝切除术开腹肝切除术治疗

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外用溃疡散和康复新液治疗复发性阿弗他溃疡疗效观察

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浙江医学教育 2014年第2期13卷 61-63页

作者：董显进董啸朱岐振玉环县人民医院浙江玉环317600

目的:探讨外用溃疡散和康复新液单用与联用治疗复发性阿弗他溃疡的临床疗效。方法:将120例复发性阿弗他溃疡患者按照随机数字表法分成A组、B组及C组,每组40例,A组单用外用溃疡散外涂治疗,3次/天,B组单用康复新液口服治疗,3次/天,C组给... 详细信息

目的:探讨外用溃疡散和康复新液单用与联用治疗复发性阿弗他溃疡的临床疗效。方法:将120例复发性阿弗他溃疡患者按照随机数字表法分成A组、B组及C组,每组40例,A组单用外用溃疡散外涂治疗,3次/天,B组单用康复新液口服治疗,3次/天,C组给予外用溃疡散和康复新液联用治疗,疗程均为7天。比较3组患者临床疗效及平均溃疡期。结果:A组与B组治疗后总有效率、平均溃疡期、疼痛指数比较差异无统计学意义(P>0.05),C组总有效率明显优于A组及B组,平均溃疡期明显短于A组及B组,疼痛指数明显小于A组与B组(P均<0.05)。结论:外用溃疡散和康复新液联用,可明显增强临床疗效,减轻疼痛,促进溃疡愈合。

关键词：外用溃疡散康复新液复发性阿弗他溃疡

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Locked nucleic acids based DNA circuits with ultra-low leakage

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Nano Research 2023年第1期16卷 865-872页

作者： Hao Hu Liquan Liu Lei Zhang Wei Zhang Kejun dong Bei Yan Yaoqin Mu Mengdi Shi Longjie Li xianjin Xiao Institute of Reproductive Health Tongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China Union Hospital Tongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430022China School of Life Science and Technology Wuhan Polytechnic UniversityWuhan 430023China

DNA circuits based on toehold-mediated DNA strand displacement reaction are powerful tools owing to their programmability and ***,performance and practical application of the circuits are greatly restricted by leakage,which refers to the fact that there is no input(invading strand)in the circuit,and the output signal is still ***,we constructed locked nucleic acids-based DNA circuits with ultra-low *** binding affinity of LNA(locked nucleic acid)-DNA/LNA suppressed the leakage by inhibiting the breathing *** on the strategy,we have built various low-leakage DNA circuits,including translator circuit,catalytic hairpin assembly(CHA)circuit,entropy-driven circuit(EDC),and seesaw *** importantly,our strategy would not affect the desired main reactions:The output signal remained above 85%for all tested circuits,and the signalto-noise ratios were elevated to 148.8-fold at the *** believe our strategy will greatly promote the development and application of DNA circuits-based DNA nanotechnology.

关键词： DNA circuits leakage locked nucleic acid DNA strand displacement DNA nanotechnology

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Thermodynamics-guided two-way interlocking DNA cascade system for universal multiplexed mutation detection

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Chinese Chemical Letters 2022年第1期33卷 334-338页

作者： Wei Zhang Liquan Liu Yangwei Liao Wan Shu Xiaofeng Tang Kejun dong Zhihao Ming xianjin Xiao Hongbo Wang Department of Obstetrics and Gynecology Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430022China Institute of Reproductive Health Tongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China

Detection of point mutations in driver genes is of great significance for the early diagnosis,treatment,and prognostic evaluation of ***,current detection methods do not offer versatility,specificity,and rapid performance ***,multiple mutation detection processes are necessary,which results in long processing times and high *** this study,we developed a thermodynamics-guided two-way interlocking DNA cascade system for universal multiplexed mutation detection(TTI-CS).This strategy is based on the DNA probe,which changes the thermodynamic balance of the DNA cascade by the designed bubble structure,thereby achieving a good distinction between mutant and wild-type *** designed method greatly shortens the detection time through two-way *** addition,this method only changes two inexpensive trigger and bridge sequences,which replace the specific and expensive nucleic acid probes used in analyses based on traditional DNA probe methods,thereby enabling multiple *** performed the detection of synthetic single-stranded DNA for the five mutation points and successfully detected in endometrial cancer *** detection limit of this method is0.1%,which better meets the needs of clinical low-abundance multiple mutation ***,TTI-CS is currently one of the best methods for detecting multiple mutation detections.

关键词： Nucleic acid probes DNA cascade system Universality Low-abundance Multiple mutation detection

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DNA origami-based nano-hunter enriches low-abundance point mutations by targeting wild-type gene segments

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Chinese Chemical Letters 2022年第4期33卷 2052-2056页

作者： Longjie Li Kejun dong Xinyu Wang Meizhou Zhang Jun Li Andreas KNussler xianjin Xiao Hongbo Wang Yuzhou Wu School of Life Science and Technology Wuhan Polytechnic UniversityWuhan 430023China Hubei Key Laboratory of Bioinorganic Chemistry and Materia Medica Hubei Engineering Research Center for Biomaterials and Medical Protective MaterialsSchool of Chemistry and Chemical EngineeringHuazhong University of Science and TechnologyWuhan 430074China Department of Obstetrics and Gynecology Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430022China Institute of Reproductive Health Tongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China Department of Breast Surgery Second Hospital of Jilin UniversityChangchun 130041China Siegfried Weller Institute for Trauma Research Eberhard-Karls-Universitat TubingenSchnarrenbergstr.95D-72076TubingenGermany

Point mutations can be used as biomarkers to perform diagnosis for diseases. In this study, a nanorobot for low-abundance point mutation enrichment was constructed using DNA origami. The novel design achieved limits of detection of 0.1% and 1% for synthesized DNA samples and clinical gene samples, respectively. Resettability was a key property of this method, which also involved a simpler process, lower cost and shorter detection duration than traditional enrichment methods. This novel DNA nanorobot may enable the detection of tumor markers, potentially facilitating early cancer diagnosis.

关键词： Low-abundance point mutation Cancer diagnosis DNA nano-hunter Enrichment Resettability

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A new method for evaluating the quality of single sperm by detecting reactive oxygen species

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Chinese Chemical Letters 2023年第2期34卷 306-310页

作者： Hao Hu Bei Yan Wei Zhang Wenbo Yan Liquan Liu Xiaofeng Tang Kejun dong Longjie Li xianjin Xiao Chengliang Xiong Institute of Reproductive Health Tongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China Sperm Bank General Hospital of Ningxia Medical UniversityYinchuan 750004China Department of Obstetrics and Gynecology Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430022China School of Life Science and Technology Wuhan Polytechnic UniversityWuhan 430023China Reproductive Medicine Center Tongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China Hubei Province Human Sperm Bank Wuhan 430030China

Sperm damage caused by reactive oxygen species(ROS) is one of the main causes of male ***, the level of ROS in sperm is an important indicator for the diagnosis and prognosis of male infertility. Herein, we constructed a single sperm ROS detection method(SSRDM) with an optical microprobe fabricated via focused ion beam process. The micro-probe is used to separately excite fluorescence in the sperm and the area around the sperm after ROS staining, and the difference in fluorescence values can reflect the level of ROS in the sperm. We collected 102 semen samples and 72 of them were divided into asthenozoospermia and non-asthenozoospermia groups. SSRDM and flow cytometry were used to detect the ROS levels of the two groups. The results of SSRDM showed that the ROS levels of asthenozoospermia group were higher than that of non-asthenozoospermia group(P = 0.002), while the results of flow cytometry indicated no difference(P = 0.152). In terms of ROS levels, compared with flow cytometry, SSRDM has a stronger ability to distinguish between those two groups, providing a reliable basis for assessment of sperm quality. Another 30 semen samples were used to investigate temperature and temporal variability of SSRDM to ensure the stability and accuracy of this method. Overall, we have developed a method that can quantitatively detect fluorescent substances in sperm at the single-cell level supplying evidence for diagnosis and prognosis of male infertility.

关键词： Reactive oxygen species Single sperm Male infertility Microprobe Flow cytometry

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The effects of soil sand contents on characteristics of humic acids along soil profiles

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Acta Geochimica 2016年第3期35卷 251-261页

作者： Xinyue Di Hui dong xianjin An Haiming Tang Baohua Xiao State Key Laboratory of Environmental Geochemistry Institute of GeochemistryChinese Academy of SciencesGuiyang 550081China Graduate University of Chinese Academy of Sciences Beijing 100049China

It is generally accepted that the compositions and properties of soil organic matter(SOM) are in?uenced by many factors. In order to reveal the effects of soil texture on characteristics and dynamics of SOM and its sub-fraction,humic acid(HA), along two soil pro?les, a yellow soil pro?le and a purplish soil pro?le, under the same climate and vegetation conditions were determined. Results indicate that the decomposition and humi?cation degrees of SOM and HA of the purplish soils are higher than those of the corresponding yellow soils indicated by A/O–A ratios of HAs, TOCs and HA yields of bulk soil samples, nevertheless, the development degree of the purplish soil is lower than that of the yellow soil. The variations of E_4/E_6 ratios of HAs along the soil pro?les indicate the overall molecular sizes of HAs decreased downward along the soil pro?les.A/O–A ratios of HAs decreased downward along both the soil pro?les indicate that humi?cation processes decrease downward along both the soil pro?les. Leaching of SOM shows signi?cant effects on the distribution and characteristics of HAs in the yellow soil pro?le but the purplish soil pro?le, which is consistent with the higher hydrophobicity of HAs in purplish soils, shows that the distribution characteristics of SOM along the soil pro?les are a complex result of the combination of soil texture and characteristics of SOM itself. The remarkably different sand contents are concluded tentatively as one of reasons to the different distributions and dynamics of HAs along the soil pro?les,however, to profoundly understand the evolution and transport of SOM along soil pro?les needs more researches.

关键词：土壤剖面腐殖酸含砂量特性土壤有机质腐殖化程度土壤质地剖面显示

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Research progress on aging of organic pollutants in geosorbents:a review

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Acta Geochimica 2017年第1期36卷 27-43页

作者： xianjin An Baohua xiao Xinyue Di Hui dong Haiming Tang State Key Laboratory of Environmental Geochemistry Institute of Geochemistry Chinese Academy of SciencesGuiyang 550002 China University of Chinese Academy of Sciences Beijing 100049China

Geosorbents are the main host of anthropogenic organic pollutants and play a vital role in their fate and transport in the natural *** and sediment are the most common and abundant geosorbents in the natural environment;their interactions with organic pollutants,especially sorption and desorption processes,have been extensively studied from the perspectives of thermodynamics and ***,the aging of organic pollutants in geosorbents has drawn increased attention,leading to an improved understanding of interactions between organic pollutants and geosorbents and informing remediation *** has been deemed important in accurately assessing ecologic and health risks of organic pollutants,and both positive and negative impacts have been reported in studies of natural and artificial *** paper summarizes recent research progress on organic pollutant aging in geosorbents,including related mechanism research,influence factors,bioavailability assessments,and biological and physicochemical remediation of aged organic *** also discuss issues in the current research and bring forward suggestions for future study.

关键词： Geosorbent 老化效果 Bioavailability 器官的污染物质 Supergene 过程

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Combined pollution of arsenic and Polymyxin B enhanced arsenic toxicity and enriched ARG abundance in soil and earthworm gut microbiotas

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Journal of Environmental Sciences 2021年第11期33卷 171-180页

作者： Lyu Li dong Zhu Xingyun Yi Jianqiang Su Guilan Duan xianjin Tang Yongguan Zhu State Key Lab of Urban and Regional Ecology Research Center for Eco-Environmental SciencesChinese Academy of SciencesBeijing 100085China University of Chinese Academy of Sciences Beijing 100049China Key Laboratory of Urban Environment and Health Institute of Urban EnvironmentChinese Academy of SciencesXiamen 361021China Institute of Soil and Water Resources and Environmental Science Zhejiang UniversityHangzhou 310058China

Polymyxin B(PMB)is considered as the last line of antibiotic defense available to *** environmental effects of the combined pollution with PMB and heavy metals and their interaction mechanisms are *** explored the effects of the combined pollution with PMB and arsenic(As)on the microbial composition of the soil and in the earthworm gut,as well as the spread and transmission of antibiotic resistance genes(ARGs).The results showed that,compared with As alone,the combined addition of PMB and As could significantly increase the bioaccumulation factor and toxicity of As in earthworm tissues by 12.1%and 16.0%,*** treatment could significantly increase the abundance of Actinobacteria in the earthworm gut(from 35.6%to 45.2%),and As stress could significantly increase the abundance of Proteobacteria(from 19.8%to 56.9%).PMB and As stress both could significantly increase the abundance of ARGs and mobile genetic elements(MGEs),which were positively correlated,indicating that ARGs might be horizontally *** inactivation of antibiotics was the main resistance mechanism that microbes use to resist PMB and As *** analysis showed that PMB and As might have antagonistic effects through competition with multi-drug resistant *** combined pollution by PMB and As significantly promoted the relative abundance of microbes carrying multi-drug resistant ARGs and MGEs,thereby increasing the risk of transmission of *** research advances the understanding of the interaction mechanism between antibiotics and heavy metals and provides new theoretical guidance for the environmental risk assessment and combined pollution management.

关键词： Arsenic Soil Earthworm gut microbiotas Polymyxin B Antibiotic resistance genes

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