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New insights into the role of spermine in enhancing the antioxidant capacity of rat spleen and liver under oxidative stress

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Animal Nutrition 2017年第1期3卷 85-90页

作者： xianjian wu Wei Cao Gang Jia Hua Zhao Xiaoling Chen Caimei wu Jiayong Tang Jing Wang Guangmang Liu Institute of Animal Nutrition Sichuan Agricultural University Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education Maize Research Institute Sichuan Agricultural University

Oxidative stress can damage cellular antioxidant defense and reduce livestock production *** is a ubiquitous cellular component that plays important roles in stabilizing nucleic acids,modulating cell growth and differentiation, and regulating ion channel activities. Spermine has the potential to alleviate the effects of oxidative stress. However, to date no information is available about the effect of spermine administration on antioxidant property of the liver and spleen in any mammalian in vivo system. This study aims to investigate the protective effect of spermine on rat liver and spleen under oxidative stress. Rats received intragastric administration of either 0.4 μmol/g body weight of spermine or saline once a day for 3 days. The rats in each treatment were then injected with either diquat or sterile saline at 12 mg/kg body weight. Liver and spleen samples were collected 48 h after the last spermine *** showed that regardless of diquat treatment, spermine administration significantly reduced the malondialdehyde(MDA) content by 23.78% in the liver and by 5.75% in the spleen, respectively(P < 0.05).Spermine administration also enhanced the catalase(CAT) activity, anti-hydroxyl radical(AHR) capacity and glutathione(GSH) content by 38.68%, 15.53% and 1.32% in the spleen, respectively(P< 0.05). There were interactions between spermine administration and diquat injection about anti-superoxide anion(ASA),AHR capacity, CAT activity, GSH content, and total antioxidant capacity(T-AOC) in the liver and about ASA capacity and T-AOC in the spleen of weaned rats(P < 0.05). Compared with the control group, spermine administration significantly increased the AHR capacity, CAT activity, GSH content, and T-AOC by 40.23%,31.15%, 30.25%, 35.37% in the liver, respectively(P < 0.05) and increased the T-AOC by 8% in the spleen of weaned rats(P < 0.05). Compared with the diquat group, spermine + diquat group significantly increased ASA capacity by 15.63

关键词： Spermine Oxidative stress Antioxidant capacity Spleen Liver

Roles of dietary supplementation with arginine or N-carbamylglutamate in modulating the inflammation, antioxidant property, and m RNA expression of antioxidant-relative signaling molecules in the spleen of rats under oxidative stress

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Animal Nutrition 2018年第3期4卷 322-328页

作者： Weiwei Mo xianjian wu Gang Jia Hua Zhao Xiaoling Chen Jiayong Tang Caimei wu Jingyi Cai Gang Tian Jing Wang Gangmang Liu Institute of Animal Nutrition Sichuan Agricultural University Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education Maize Research Institute Sichuan Agricultural University

This study evaluated the effects of arginine(Arg) or N-carbamylglutamate(NCG) on inflammation, antioxidant property, and antioxidant-related gene expression in rat spleen under oxidative stress. A total of 52 rats were randomly distributed into 4 treatment groups with 13 replicates per group. Rats were fed a basal diet(BD) or BD supplemented with Arg or NCG for 30 days. On day 28, half of the BD-fed rats were intraperitoneally injected with sterile saline(control group), and the other half with 12 mg/kg body weight of diquat(DT; DT group). The other 2 diet groups were intraperitoneally injected with 12 mg/kg body weight of DT with either Arg(1%)(DT t Arg) or NCG(0.1%)(DT t NCG). Rat spleen samples were collected for analysis at 48 h after DT injection. Results showed that DT damaged the antioxidant defense in rats compared with the control group(P < 0.05). Compared with the DT group, the DT t Arg and DT t NCG groups manifested improved anti-hydroxyl radical, catalase, and total superoxide dismutase(T-SOD) activities, increased glutathione content(P < 0.05), and decreased malondialdehyde content(P < 0.05).Moreover, compared with the DT group, the DT t Arg and DT t NCG groups enhanced mRNA expression of superoxide dismutase(SOD), glutathione peroxidase 1(GPx1), glutathione reductase(GR), nuclear factor erythroid 2-related factor 2(Nrf2), Kelch-like ECH-associated protein 1(Keap-1), and mammalian target of rapamycin(m TOR)(P < 0.05). Both NCG and Arg significantly increased anti-inflammatory cytokine mRNA level but suppressed the pro-inflammatory cytokine mRNA expression under oxidative stress(P < 0.05). In summary, NCG and Arg effectively alleviated oxidative stress, improved the antioxidant capacity and regulated the antioxidant-related signaling molecular expression in rat spleen. N-carbamylglutamate and Arg reduced the inflammation in the spleen by mediating the gene expression of anti-inflammatory and pro-inflammatory cytokines and transforming growth factor-β(TGF

关键词： Arginine N-carbamylglutamate Antioxidant property Signaling molecule Inflammation Spleen

Arginine, N-carbamylglutamate,and glutamine exert protective effects against oxidative stress in rat intestine

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Animal Nutrition 2016年第3期2卷 242-248页

作者： Liang Xiao Wei Cao Guangmang Liu Tingting Fang xianjian wu Gang Jia Xiaoling Chen Hua Zhao Jing Wang Caimei wu Jingyi Cai Institute of Animal Nutrition Sichuan Agicultural University Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education Maize Research Institute Sichuan Agricultural University

The objective of the current study is to evaluate the effects of dietary supplementation with arginine(ARG), N-carbamylglutamate(NCG), and glutamine(GLN) on rat intestinal morphology and antioxidant status under oxidative stress. Rats were fed for 30 d with one of the following iso-nitrogenous diets:basal diet(BD), BD plus 1% ARG, BD plus 0.1% NCG, and BD plus 1% GLN. On day 28, half of the rats fed BD were intraperitoneally injected with 12 mg/kg body weight of diquat(DT; i.e., the DT group) and the other half was intraperitoneally injected with sterile solution(i,e., the control group). The other diet groups were intraperitoneally injected with 12 mg/kg body weight of DT(i,e., DT + 1% GLN [DT + GLN],DT + 1% ARG [DT + ARG], and DT + 0.1% NCG [DT + NCG]). Rat jejunum samples obtained at 48 h after DT injection were analyzed. Results showed that DT significantly decreased catalase(CAT) activity and glutathione(GSH) content by 58.25% and 56.57%, respectively, and elevated malondialdehyde(MDA)content and crypt depth(CD) by 19.39% and 22.13%, respectively, in the jejunum(P < 0.05, relative to the control group). Compared with the DT group, the DT + GLN group exhibited significantly improved villus height(VH), villus width(VW), villus surface area(VSA), CD and total antioxidant capacity(T-AOC) activity(P < 0.05); the DT + ARG group exhibited significantly increased the ratio of VH to CD(H:D) and TAOC activity(P < 0.05); the DT + GLN, DT + ARG and DT + NCG groups exhibited significantly enhanced CAT activity and GSH content as well as decreased MDA content(P < 0.05). Moreover, VH, VW, VSA, CD and GSH content in the DT + GLN group were higher whereas MDA content was lower compared with the corresponding values observed in both the DT + ARG and the DT + NCG groups(P < 0.05). The H:D ratio in the DT + ARG group significantly increased compared with that in the DT + NCG and DT + GLN groups(P < 0.05). Collectively, this study suggested that dietary supplementation with 1% GLN,

关键词： Arginine Glutamine N-Carbamylglutamate Oxidative stress Jejunum Antioxidant status

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耐磨蚀钢BMS1400焊接性能研究

热加工工艺 2023年第1期52卷 113-118页

作者：宋凤明温东辉伍先见宝山钢铁股份有限公司研究院上海201900 上海连山金属材料有限公司技术部上海200940

采用埋弧焊方法对比了不同强度焊丝焊接耐磨蚀钢BMS1400的焊接性能。较低强度的50、60 kg焊丝焊接样板具有相近的接头拉伸强度,抗拉强度均超过了600 MPa;80 kg焊丝的接头抗拉强度则超过760 MPa。焊接样板均具有良好的冷弯性能,但在冷弯... 详细信息

采用埋弧焊方法对比了不同强度焊丝焊接耐磨蚀钢BMS1400的焊接性能。较低强度的50、60 kg焊丝焊接样板具有相近的接头拉伸强度,抗拉强度均超过了600 MPa;80 kg焊丝的接头抗拉强度则超过760 MPa。焊接样板均具有良好的冷弯性能,但在冷弯位置存在应变集中,高强度焊丝有利于降低焊接接头的应变集中。焊接接头的低温冲击韧性随着焊丝强度的提高而提高。在19.6~22.3 kJ/cm焊接热输入量条件下,50、60 kg焊丝的焊接接头均存在明显的焊接软化和硬度峰值;而80 kg焊丝焊接的接头硬度更为均匀。焊接热影响区的组织与距离焊缝的远近有关,靠近焊缝及HAZ中心均为板条状铁素体+少量马氏体组织,而靠近母材则为类似高温回火的带状铁素体+珠光体组织。采用更高强度焊丝的样板具有更好的低温冲击韧性,并且应变集中降低,从而获得更好的焊接接头性能。

关键词：耐磨蚀钢焊接性能埋弧焊

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行动者网络视角下贵州民族传统节庆文化品牌构建与传播研究——以雷山苗年为例

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新闻研究导刊 2022年第14期13卷 63-65页

作者：廖慧罗奥搏吴显剑贵州财经大学贵州贵阳550025

苗年是贵州雷山苗族同胞最重要的节日,2008年经国务院批准列入第二批国家级非物质文化遗产名录。几十年来,在政府的主导与支持下,苗年成为雷山县的知名品牌,成为当地文化发展、经济增长的重要助推器。文章通过田野调查发现,雷山苗年品... 详细信息

苗年是贵州雷山苗族同胞最重要的节日,2008年经国务院批准列入第二批国家级非物质文化遗产名录。几十年来,在政府的主导与支持下,苗年成为雷山县的知名品牌,成为当地文化发展、经济增长的重要助推器。文章通过田野调查发现,雷山苗年品牌的构建与传播过程不单是政府、企业等“人”的行动,而是由“人”与“物”所共同建构的。由此,从拉图尔行动者网络理论的视角,聚焦雷山苗年品牌构建与传播过程,发现整合人类行动者和非人类行动者是关键,需要改变创新环境、合作方式和优化利益分配模式来优化其发展动力机制。文章最后进一步提出苗年品牌的发展需要挖掘民族节庆文化内涵建立独特品牌识别,以及通过跨媒体整合传播来扩大民族节庆品牌知名度。

关键词：雷山苗年行动者网络品牌构建品牌传播品牌识别

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Q890超高强钢GMAW焊接工艺

电焊机 2015年第5期45卷 55-58页

作者：贾军杨益清李好生伍先见李光波易飞聂江兵昆山中冶宝钢焊接材料有限公司江苏苏州215333

Q890及以上级别的超高强钢在国内应用越来越广泛。由于该级别钢材有强度极高且碳当量高等特点,使其焊接工艺的制定非常困难。Q890钢具有一定的冷裂倾向,采用BHG-5焊丝焊接时,在热输入量不超过1.472 k J/mm、焊道间温度不超过200℃的条件... 详细信息

Q890及以上级别的超高强钢在国内应用越来越广泛。由于该级别钢材有强度极高且碳当量高等特点,使其焊接工艺的制定非常困难。Q890钢具有一定的冷裂倾向,采用BHG-5焊丝焊接时,在热输入量不超过1.472 k J/mm、焊道间温度不超过200℃的条件下,焊缝金属的综合力学性能能够满足Q890钢板的焊接技术要求;同时焊后热处理对焊接接头焊缝金属的力学性能有较大影响,推荐焊后热处理温度不超过480℃。

关键词： Q890 超高强钢焊接工艺

PTCS术与传统手术治疗复发性胆管结石60例临床分析

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右江民族医学院学报 2021年第5期43卷 636-638,642页

作者：吴贤建路远卓臣义李文川许佐明谭川浦涧右江民族医学院附属医院肝胆外科广西百色533000

目的探讨在复发性胆管结石的治疗中经皮经肝胆道镜取石术(PTCS)和传统手术方案的治疗优势。方法选取右江民族医学院附属医院肝胆外科所收治的复发性胆管结石患者60例,随机分成两组。治疗实验组患者采用PTCS术式治疗,对照组患者采用传统... 详细信息

目的探讨在复发性胆管结石的治疗中经皮经肝胆道镜取石术(PTCS)和传统手术方案的治疗优势。方法选取右江民族医学院附属医院肝胆外科所收治的复发性胆管结石患者60例,随机分成两组。治疗实验组患者采用PTCS术式治疗,对照组患者采用传统术式治疗。对两组患者的疗效评估包含术中出血量、术后结石残余率、术后并发症发生比例、手术全程耗时、术后肛门排气恢复时间、疼痛等级。结果与对照组相比,治疗实验组患者手术出血量减少(P<0.05)、手术时间明显缩短(P<0.05)、结石残余率降低(P<0.05);治疗实验组患者术后肛门排气恢复时间明显缩短(P<0.05),术后第1天术口疼痛程度轻(P<0.05)。结论PTCS术与传统手术方案相比具有安全、可行,操作简单,取净结实率高的特点,在临床应用中能够发现患者创伤小、康复快、并发症少的优势,在肝胆外科微创治疗的工作中具有一定的推广空间。

关键词：复发性胆管结石经皮经肝胆道镜取石术 B超定位传统手术

大黄灵仙方对胆汁IL-6及TNF-α水平的影响研究

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右江民族医学院学报 2020年第6期42卷 701-704页

作者：吴贤建唐乾利路远卓臣义李文川许佐明浦涧右江民族医学院研究生学院广西百色533000 右江民族医学院附属医院肝胆外科广西百色533000

目的探讨中药大黄灵仙方对胆石症患者胆汁白介素-6(IL-6)及肿瘤坏死因子-α(TNF-α)表达的影响。方法采集2017年12月—2019年12月在我科住院行“胆总管切开取石+T型管外流术”的胆石症患者90例,其中观察组60例,对照组30例,两组均在术后... 详细信息

目的探讨中药大黄灵仙方对胆石症患者胆汁白介素-6(IL-6)及肿瘤坏死因子-α(TNF-α)表达的影响。方法采集2017年12月—2019年12月在我科住院行“胆总管切开取石+T型管外流术”的胆石症患者90例,其中观察组60例,对照组30例,两组均在术后1周左右出院并嘱正常饮食并适当休息,观察组在术后第7 d时开始服用大黄灵仙方1个疗程(14 d)。两组T型管留置21 d后经造影检查确认无结石残留后拔除,若有残留者留置T管待术后第8周经T管窦道行胆道镜并钬激光碎石及取石治疗。两组均在无菌操作下分别采集患者术后第1 d、7 d、14 d、21 d清晨的空腹新鲜胆汁样本5 ml,应用酶联免疫方法检测两组胆汁标本中IL-6、TNF-α的蛋白浓度。结果术后第1 d、7 d、14 d、21 d IL-6观察组分别为(155.81±10.80)pg/ml、(122.27±6.64)pg/ml、(73.36±5.71)pg/ml、(18.15±3.27)pg/ml,对照组分别为(157.42±13.16)pg/ml、(121.52±6.21)pg/ml、(106.10±7.96)pg/ml、(38.40±3.71)pg/ml,第1 d、7 d两组比较差异无统计学意义(P>0.05);第14 d、21 d两组比较差异有统计学意义(P0.05);第14 d、21 d两组比较差异有统计学意义(P<0.05)。结论大黄灵仙方降低胆石症患者胆汁IL-6、TNF-α的浓度。

关键词：胆石症胆汁大黄灵仙方白细胞介素6 肿瘤坏子因子α

急性低氧诱导人肝癌细胞差异表达基因的筛选及其功能

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山东医药 2020年第1期60卷 1-4页

作者：路远韦花媚谭川吴贤建邵泽生伍毅许佐明李文川浦涧右江民族医学院附属医院·广西肝胆疾病临床研究中心广西百色533000 右江民族医学院

目的探讨急性低氧诱导人肝癌细胞(Huh-7、Hep3B)差异表达基因的筛选及其功能。方法常规培养Huh-7、Hep3B细胞,随机取部分细胞置于常氧条件(空气、5%CO 2)下培养,取部分细胞置于低氧条件(1%O 2、5%CO 2、94%N 2)下培养,均培养20 h后收样... 详细信息

目的探讨急性低氧诱导人肝癌细胞(Huh-7、Hep3B)差异表达基因的筛选及其功能。方法常规培养Huh-7、Hep3B细胞,随机取部分细胞置于常氧条件(空气、5%CO 2)下培养,取部分细胞置于低氧条件(1%O 2、5%CO 2、94%N 2)下培养,均培养20 h后收样。采用Gene Chip Clariom S Array芯片筛选急性低氧诱导和常氧处理细胞的差异表达基因,筛选条件为错误发现率1;用Kolmogorov-Smirnov和KOBAS version 2.0对差异表达基因进行基因本体论功能分析和京都基因与基因组百科全书通路分析。结果与常氧处理的细胞相比,急性低氧诱导的Huh-7细胞中差异表达基因共计392个,其中表达上调基因266个,表达下调基因126个;急性低氧诱导的Hep3B细胞中,差异表达基因共计2260个,其中表达上调基因847个,表达下调基因1413个。急性低氧诱导的Huh-7、Hep3B细胞共同的差异表达基因共177个,表达上调基因127个,表达下调基因50个。急性低氧诱导的Huh-7细胞中差异表达基因主要与小分子代谢、有机物生物合成、细胞应激反应相关,主要富集在糖酵解糖异生、MAPK、细胞周期、癌症相关信号通路上。急性低氧诱导的Hep3B细胞中差异表达基因主要与小分子代谢、磷酸化代谢、细胞生长与凋亡等相关,主要富集在细胞周期、神经退行性疾病、细胞凋亡以及磷酸化调控相关信号通路上。两株细胞系共同的差异表达基因表达上调最明显的3个基因TXNIP、ATF3、SLC6A8均与肝癌发生相关。结论成功从急性低氧诱导人肝癌细胞中筛选出差异表达基因,呈高表达的基因可能与肝癌发生相关。

关键词：肝癌低氧人肝癌细胞系基因芯片技术差异表达基因