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华南家鸡MHC-B区域复合微卫星位点LEI0258的遗传多样性与进化研究

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畜牧兽医学报 2016年第11期47卷 2175-2183页

作者：黄勋和李丽芝张金枫何丹林张细权陈洁波李威娜杜炳旺钟福生嘉应学院生命科学学院梅州514015 华南农业大学动物科学学院广州510642 广东海洋大学农学院湛江524088

旨在应用MHC-B区域复合微卫星位点LEI0258研究华南家鸡的遗传多样性与进化历史。以华南地区和邻省家鸡及商品鸡为试验材料,对微卫星位点LEI0258进行基因分型和测序,分析其遗传多样性和构建中介网络图。结果表明,580份样品共检测到LEI025... 详细信息

旨在应用MHC-B区域复合微卫星位点LEI0258研究华南家鸡的遗传多样性与进化历史。以华南地区和邻省家鸡及商品鸡为试验材料,对微卫星位点LEI0258进行基因分型和测序,分析其遗传多样性和构建中介网络图。结果表明,580份样品共检测到LEI0258的62个等位基因,长度范围为182~501bp,其中37个为首次发现。华南家鸡LEI0258保持着较高的遗传多样性水平,观察杂合度、期望杂合度和多态信息含量分别为0.813、0.960和0.957。中山沙栏鸡、文昌鸡和五华三黄鸡的遗传多样性水平明显高于广西三黄鸡。LEI0258的28个等位基因与27种已知MHC血清型相对应。基于LEI0258侧翼区变异信息的中介网络图将62个等位基因分为8条进化枝,其中3条进化枝同样存在于红原鸡中,提示华南家鸡主要起源于红原鸡。部分等位基因存在于特定鸡品种中,可作为品种鉴定的参考依据。结果提示,微卫星位点LEI0258适用于地方鸡群体遗传多样性、血清型鉴定、品种鉴定和进化研究。

关键词：主要组织相容性复合体 LEI0258 华南家鸡多态性进化血清型

Cloning and Spatiotemporal Expression Analysis of Bombyx Mori elav,an Embryonic Lethal Abnormal Visual Gene

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Cloning and Spatiotemporal Expression Analysis of Bombyx Mor...

中国蚕学会第六届青年学术研讨会

作者： Ying LIUa b Geng-Xian wanga Sheng-Xiang Zhang c Yang-Hu SIMAa and Shi-Qing XUa (a National Engineering Laboratory of Modern Silk Department of Applied Biology Medical College Soochow University Suzhou Jiangsu 215153 People’s Republic of China bBioengineering Department Jiangsu Polytechnic College of Agriculture and Forestry Zhenjiang Jiangsu 215006 People’s Republic of China c College of Forestry Shandong Agricultural University Taian Shandong 215153 People’s Republic of China)

Embryonic lethal abnormal visual（elav） is a lethal gene in Drosophila inducing the abnormal development and function of nervous system. We cloned a Bm-elav gene by bioinformatics and biological experiment,based on sequence of ELAV protein and dbEST of Bombyx mori. The full-length of Bm-elav cDNA is 1498 bp,contains a 906 bp open read frame（ORF） encoding a precursor of 301 amino acid residues with a calculated molecular weight of 34 kDa and pI of 8.99. Bm-ELAV protein precursor contains three RNA recognition motifs（RRM） in 24-91,110-177 and 222-295 bit amino acid residues respectively,and belongs to RNA-binding protein family. Bm-ELAV shared varying positives,ranging from 56% to 60%（Identities from 41% to 45%） ,with RRM from other species of Xenopus tropicalis,Apis mellifera,Tribolium castaneum,Branchiostoma belcheri and Drosophila. Gene localization indicated that Bm-elav is a single-copy gene,gene mapping within 12-chromosome from 7916.68 knt to 7918.16 knt region of nscaf2993. Spatiotemporal expressions pattern analysis revealed that Bm-elav expressed higher in most tested tissues and developmental stages in whole generation,such as silk gland,fat body,midgut,hemopoietic organ and ovary,but almost no expression in terminated diapause eggs. This suggested that the expression of Bm-elav in early developmental embryonic stages might induce abnormal development like in Drosophila. Cloning of the Bm-elav gene enables us to test its potential role in controlling pests by transferring the gene into field Lepidopteran insects in the future.

关键词： Bombyx mori embryonic lethal abnormal visual(elav) gene clone gene localization spatiotemporal expressions

gltS基因缺失对禽致病性大肠杆菌生物学特性的影响

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中国预防兽医学报 2023年第7期45卷 690-697页

作者：王利丽张春晓张闫孙欣艺侯冠欣陶勇赵奇张志强史秋梅河北科技师范学院河北省预防兽医重点实验室河北秦皇岛066004

为研究谷氨酸转运蛋白GltS编码基因对禽致病性大肠杆菌(APEC)生物学特性的影响,本研究利用λ-Red同源重组方法构建APEC CE129(WT)株gltS基因缺失株CE129ΔgltS(KO)和基因回补株CE129ΔgltS/gltS (RS),并均经PCR及测序鉴定,结果显示,glt... 详细信息

为研究谷氨酸转运蛋白GltS编码基因对禽致病性大肠杆菌(APEC)生物学特性的影响,本研究利用λ-Red同源重组方法构建APEC CE129(WT)株gltS基因缺失株CE129ΔgltS(KO)和基因回补株CE129ΔgltS/gltS (RS),并均经PCR及测序鉴定,结果显示,gltS基因缺失株及回补株均正确构建。通过连续10 h测定各菌株OD600nm值绘制WT、KO、RS菌株的生长曲线,利用梅里埃自动生化鉴定系统测定3株菌的生化特性;利用K-B纸片法检测3株菌对8类16种药物的敏感性;利用半固体培养基检测3株菌的运动能力。结果显示,3株菌的生长特性、生化特性、药敏特性和运动能力均无明显差异。利用细菌平板计数法计算检测WT与KO菌株的体外竞争指数(CI),分析KO菌株是否致弱。利用结晶紫染色法测定3株菌的生物被膜(BF)的形成能力,并利用刚果红琼脂板及含荧光增白剂的琼脂板分别检测菌株BF中curli菌毛及纤维素的形成情况。结果显示,WT与KO菌株的体外CI为0.32,表明KO菌株轻度致弱。BF形成能力检测结果显示,相对于WT、RS菌株,KO菌株BF的形成能力极显著降低(P<0.001),且KO菌株BF的主要组成成分curli菌毛有明显变化,表明gltS基因参与APEC BF中curli菌毛的形成,而3株菌在含荧光增白剂琼脂板的荧光强度均一致,表明gltS基因与APEC BF中纤维素的形成无关。采用细菌计数法统计WT、KO、RS菌株在6种应激条件下的生存率,结果显示,除氧化应激条件下3株菌的生存率无显著差异外,在酸、碱、热、铁饥饿应激环境中,与WT和RS菌株相比,KO菌株的生存率均极显著降低(P<0.001、P<0.0001)。在50 mg/L、100 mg/L NaNO2中,KO菌株的生存率显著降低(P<0.01);在150 mg/L NaNO2中,KO菌株的生存率极显著降低(P<0.001)。采用菌落计数法测定WT和KO菌株抗不同浓度血清的杀菌作用,结果显示,在高浓度的血清中(50%~100%),WT、KO菌的数量显著高于DH5α对照菌株(P<0.05);但在不同浓度血清中KO菌的数量与WT菌的数量差异不显著。本研究结果首次证实gltS基因参与APEC部分生物学特性的形成,为进一步阐释gltS基因功能及该菌的致病机制奠定了基础。

关键词：禽致病性大肠杆菌 gltS基因缺失生物学特性

Textured AZO films by magnetron sputtering and ion beams post-treatment

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Textured AZO films by magnetron sputtering and ion beams pos...

第十五届全国等离子体科学技术会议

作者： F.wanga,b~,M.Z.Wua,Y.Y.Wangd,Y.M.Yud,X.M.Wua.b~ ,L.J.Zhugeb,c a Department of physics,Soochow University,Soochow 215006,China b The Key Laboratory of Thin Films of Jiangsu,Soochow University,Soochow 215006,China c Analysis and Testing Center,Soochow University,Soochow 215006,China d Wenzheng College of Soochow University,Soochow 215006,China

A method to achieve rough AZO films by ion beam post treatment is introduced in this *** structural and electrical properties have been analyzed as a function of the ion beam by a series of characterization techniques,including atomic force microscope(AFM),scanning electron microscope(SEM) and Hall effect *** treatment,the sharp peaks are flattened,the surface becomes symmetrical,and with the increase of ion beam,the value of RMS decreases from 13.25nm to *** ion beam is 30mA,the film has the best surface morphology of pyramid-like,the surface is almost uniformly covered by craters with diameters of about 200nm and depths of about 80nm,and no sharp peak was found. Besides,the sheet resistance remains almost the same before and after the treatment, and because of the evaporation of AZO,a slight decrease in resistivity is found.

关键词：

High-Power VCSELs Single Devices and 2-D Arrays

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High-Power VCSELs Single Devices and 2-D Arrays

20th Congress of the International Commission for Optics

作者： Yanfang Suna,b, Yongqiang Ninga, Te Lia,b, Xiaonan Shana,b, Guoguang Lua,b, Chunfeng Hea,b, Li Qina, Changling Yana, Yun Liua, Lijun wanga a Key Laboratory of Excited State Processes, Changchun Institute of Optics, Fine Mechanics and Physics, Chinese Academy of Sciences, Changchun, 130033, China b Graduate school of Chinese Academy of Sciences, Beijing, 100039, China

The high power bottom-emitting vertical-cavity surface-emitting lasers (VCSELs) and laser arrays emitting at 980 nm are reported. Extensive investigations on size scaling behavior of thermal propertie

关键词： VCSELs 2-D arrays high power output power wavelength far-filed

Synthesis and biological evaluation of new peroxo-bridged diosgenin derivatives

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Chinese Herbal Medicines 2018年第1期10卷 54-58页

作者： tian-xin xie fu-hao chu wen-qiang yan bing xu jing chen rui zhao yu-zhong zhang peng-long wang hai-min lei school of chinese materia medica beijing university of chinese medicineBeijing 100102China school of education tibetan traditional medical collegelhasa 850000china department of pathology beijing university of chinese medicineBeijing 100102China

Objective： In order to find lead compound with anti-HBV activity from peroxo-bridged diosgenin deriva- tives obtained with Eosin Y as the photosensitizer. Method： Eosin Y was used as the photosensitizer to activate the oxygen in the air to synthesize novel diosgenin derivatives with peroxo-bridge. The structures of synthesized compounds were identified by NMR and HR-MS. Their cytotoxicity and antihepatitis B activity were evaluated via MTS assay and ELISA method, respectively. Results： Six diosgenin derivatives were synthesized, three of which contained peroxo-bridge, and their structures were confirmed by spectroscopy. It showed that 5a,8a-peroxo-6-alkenyl-diosgenin （7） could suppress the production of HBsAg on transfected HepG2.2.15 cells at low-toxic concentration and the in- hibition rate on HepG2.2.15 cells was 18.28% at 12.50 μg/mL, better than that of 3TC （7.30% at 12.50 μg/mL） and others. Conclusion： Due to its lower cytotoxicity and potential anti-hepatitis B activity, compound 7 could be developed as the promising candidate of anti-hepatitis B drug. It also indicated that the peroxo-bridged derivatives had potential biological values for developing clinical agents.

关键词： anti-hepatitis B diosgenin Eosin Y Peroxo-bridged derivatives

口服避孕药与克罗恩病患病风险相关性的Meta分析

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循证医学 2018年第2期18卷 106-112,121页

作者：王小云范修德周亚琼王西强许君望西安交通大学第一附属医院消化内科西安710061 西安交通大学第一附属医院感染性疾病科西安710061 西安交通大学第一附属医院心内科西安710061

目的评价口服避孕药与克罗恩病患病风险的相关性。方法计算机检索PubMed、EMBASE、中国知网和万方医学网数据库,查找关于口服避孕药与克罗恩病相关性的队列研究或病例对照研究,检索时间均为从建库至2017年11月10日。由两位研究者分... 详细信息

目的评价口服避孕药与克罗恩病患病风险的相关性。方法计算机检索PubMed、EMBASE、中国知网和万方医学网数据库,查找关于口服避孕药与克罗恩病相关性的队列研究或病例对照研究,检索时间均为从建库至2017年11月10日。由两位研究者分别严格按照纳入与排除标准筛选文献、提取资料并评价文献质量,采用Stata 13.0软件进行Meta分析。结果最终共纳入17篇文献,累计样本量共308747例。Meta分析结果显示,口服避孕药可增加克罗恩病患病风险[比值比=1.42,95%可信区间(1.24,1.61),P<0.001],现在使用口服避孕药发生克罗恩病的风险[比值比=2.07,95%可信区间(1.27,3.38),P=0.004]高于曾经使用口服避孕药[比值比=1.36,95%可信区间(1.07,1.74),P=0.027]。结论口服避孕药可增加克罗恩病的患病风险,现在使用组较曾经使用组发病风险增加,但所纳研究缺乏足够使用时间及剂量相关数据,未能进行时间-效应和剂量-效应关系的探究。

关键词：克罗恩病口服避孕药炎症性肠病 Meta分析

近红外光谱分析技术检测肝素钠原料药中的多硫酸软骨素(英文)

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近红外光谱分析技术检测肝素钠原料药中的多硫酸软骨素(英文)

科学仪器服务民生学术大会

作者： HengChang Zang a,ChunXiao Sun b,Fengshan wanga,,XuePing Guo b,Qin Dong a,Lian Li a,Pei Wang a aSchool of Pharmaceutical Sciences and National Glycoengineering Research Center,Shandong University,250012 Jinan,China b Institute of Biopharmaceuticals of Shandong Province,250101 Jinan,China

Oversulfated chondroitin sulfate(OSCS)is considered to be a contaminant in heparin active pharmaceutical ingredient(API)related to adverse reactions happened in 2008,so establishing a method for identifying and quantifying OSCS contamination in heparin API is very important for guaranteeing the quality of heparin preparations.A method for determination of OSCS in heparin API using near infrared spectroscopy(NIRS)is described in this *** establishing the method,46 calibration heparin API samples with Wt.%OSCS ranged from 0.137%to 37.001%and 20 validation samples with Wt.%OSCS ranged from 0.193%to 31.999%were prepared and their NIR spectra were *** component analysis(PCA)was used for *** using the established calibration model,5 validation samples were classified as outliers,and in four of which the Wt.%OSCS were below 1%,indicating NIRS could identify OSCS contamination in heparin API when Wt.%OSCS is larger than 1%.Partial Least Squares(PLS)was used for quantitative analysis of OSCS in heparin *** factors,correlation coefficient of calibration set(Rc),the root mean square of cross-validation(RMSECV),correlation coefficient of prediction set(Rp)and the root mean square of prediction(RMSEP)were used to evaluate the performance of the *** model was obtained with Rp=0.9803 and RMSEP= 0.02 in the full spectral region using SG-1st derivative spectral transform *** validation samples gave good repeatability *** is feasible to identify OSCS contamination of heparin API before making preparations.

关键词： OSCS Heparin API NIRS PCA Partial Least Squares

Arecoline improve aortic endothelium-dependent relaxation function in high fructose fed rats by increasing CSE expression and K+ channel open

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Arecoline improve aortic endothelium-dependent relaxation fu...

传承与发展，创湖南省生理科学事业的新高——湖南省生理科学会2011年度学术年会

作者： Guang wanga,b,Hongyan Linga,Shouhong Zhoua,Wei Zhangc,Sisi Yanga,Qixin Yaoa,Zhuqing Qia,Menghua Chena,Xing Lia,Bi Hua (aDepartment of Physiology,College of Medicine,University of South China,28 West Changsheng Road,Hengyang 421001,Hunan Province,China bDepartment of Physiology,Biological Engineering School of Anhui Provience,48 South Nongke Road,Hefei 230031,Anhui Province,China cDepartment of Physiology,College of Xiangnan,25 West Renmin Road Chenzhou 423043,Hunan Province,China)

Purpose To explore the effects of arecoline on aortic endothelium-dependent relaxation function(EDRF)in high fructose fed rats and the potential mechanisms. Methods Male Wistar rats were fed with a high fructose diet(HF group)for 12 weeks,then rats were treated with different dose of arecoline(0.5,1.0 and 5.0 mg/kg/d,intraperitoneal injection)for 4 *** fasting blood glucose(FBG)and fasting serum insulin(FSI)were measured and insulin

关键词： Arecoline,high fructose,EDRF,CSE,K+ channel.