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The Chinese-German Journal of Clinical Oncology 2008年第11期7卷 638-640页

作者： Guilong Jin Zhiwei Ding Yuxia Guo xiangxiang zhao Department of Ultrasonography Dongyang People＇s Hospital Dongyang 322100 China

Objective: To evaluate the value of identifying and diagnosing mammary carcinoma and non-lactation mastitis lump (NLM) by multicolor Doppler ultrasonography. Methods: We compared and analyzed the examination results of 69 cases of mammary carcinoma proved by surgical pathology and 22 cases of NLM before surgery by multicolor Doppler ultra-sonography. Results: The detection rates of mammary carcinoma and NLM focus by ultrasonic examining were 100%. The shape, envelope, foul line, blood stream between sound and image of two diseases were similar. Of the two diseases, the representation with slight calcification in lump, lower echo in low echo, bloodstream distribution and resistance were different. Conclusion: By analyzing the sound and image representation of mammary lump, we find color Doppler ultrasonography has significant value in identifying and diagnosing mammary carcinoma and non-lactation mastitis lump.

关键词： mammary carcinoma non-lactation mastitis lump (NLM) color Doppler ultrasonography

RNA-seq profiling the transcriptome of secondary seed dormancy in canola(Brassica napus L.)

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Chinese Science Bulletin 2014年第32期59卷 4341-4351页

作者： Fuxia Liu Xiangqiang zhao Lihua Zhang Tang Tang Changming Lu Guiming Chen Xinlong Wang Cuiping Bu xiangxiang zhao Jiangsu Key Laboratory for Eco-Agricultural Biotechnology around Hongze Lake Huaiyin Normal University School of Life Sciences Nantong University Plant science department South Dakota State UniversityBrookings SD 57006 USA Genetic Engineering and GMO Biosafety Lab of Oil Crops Research Institute Chinese Academy of Agricultural Sciences

Secondary seed dormancy(SSD)is responsible for volunteer plants in canola fields,which causes a series of problems in canola production and serves as an important trait for the environmental safety assessment of transgenic canola.A canola cultivar with strong SSD was used to establish insight into seed transcriptomes in its secondarily dormant seeds and control seeds without dormancy by RNA-seq analysis,aiming to determine the molecular ecological characterizations of SSD.A dataset(more than 4 Gb)of valid sequences was obtained from each sample,which was combined to carry out the de novo *** assembled sequences consisted of 314,261fragments with length[100 bp,including 29,740 long transcripts of length C 500 *** annotation indicated that 1,641 long transcripts could be categorized into 24 cluster of orthologous groups of proteins(COGs)and 16,515 transcripts were linked to 2,648 gene ontology(GO)*** were 452 long transcripts with significantly different expression identified by a threshold of[2-fold expression change(P\0.001)between samples,among which 343 transcripts were unambiguously homologous to Arabidopsis *** plant hormones abscisic acid and gibberellins were known as the pivotal regulators of seed dormancy and *** genes responsible for either biosynthesis or signaling of each hormone could be widely verified from the SSD transcriptome,theirs expression evidences failed to correlate with the induction of *** on the enriched terms of gene ontology and KEGG orthology,as well as the expression models of candidate genes of SSD,we proposed that fatty acid metabolism might implicate in SSD in *** information reported here may play a significant role in further understanding of environmental safety assessment of SSD in transgenic canola.

关键词：种子休眠转录物油菜田二级 Q分析转基因油菜双低油菜籽基因本体论

Rationalizing the isolation distance needed for field trials involving genetically modified rapeseed (Brassica napus L.) in China

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Chinese Science Bulletin 2013年第13期58卷 1558-1567页

作者： zhao xiangxiang TANG Tang CHEN GuiMin LIU FuXia WANG XingLong BU CuiPing LU ChangMing Key Laboratory of Oil Crop Biology of Ministry of Agriculture Oilcrops Research InstituteChinese Academy of Agricultural Sciences Jiangsu Key Laboratory for Eco-Agricultural Biotechnology around Hongze Lake Huaiyin Normal University

The isolation distance required for field trials of genetically modified (GM) rapeseed varies widely worldwide, with a 50-400 m distance in most nations contrasting with a minimum 1000-m isolation distance in China. The goal of this study was to evaluate the relevance of current regulations in China regarding the isolation distance needed for GM rapeseed trials. A pollen flow experiment was conducted based on the design of concentric circles, with the GM plants in a 20-m diameter circle at the centre, surrounded by non-GM plants to a distance 60 m from the perimeter of the circle containing GM plants. The rate of pollen flow was the highest at the isolation distance of 0.5 m, where it ranged from 2.3091% to 2.6711%. The general pattern of the pollen flow rate (y) with distance (x) was well described by the equation y = 1.3936x-0.9136 (R 2 = 0.9950). The long-distance pollen flow tested at the isolation distance of 800 m was 0.0018%, which agrees with the theoretical prediction. The results suggested that 300 m, rather than 1000 m, is a reasonable distance to ensure a tolerable threshold of pollen flow (less than 0.01%) under conditions of winter rapeseed production in China.

关键词：转基因油菜籽隔离距离现场试验中国转基因植物花粉流世界范围田间试验

Assessment on alien herbicide-resistant gene flow among crucifers by sexual compatibility

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Chinese Science Bulletin 2005年第15期50卷 1604-1611页

作者： zhao xiangxiang LU Weiping QI Cunkou PU Huiming XIA Qiuxia LU Dalei LIU Geshan WANG Youping Jiangsu Provincial Key Lab of Crop Genetics and Physiology Yangzhou University Yangzhou 225009 China Institute of Industrial Crops Jiangsu Academy of Agricultural Sciences Nanjing 210014 China College of Bioscience and Biotechnology Yangzhou UniversityYangzhou 225009 China

Cross-pollination and high cross-compatibility are frequently found in many cruciferous plants, which im-plies that there might be potential risk of alien gene flow among crucifers. In this work, the alien glyphosate-resistant gene flow between GM (Genetically modified) rapeseed vari-ety Q3 (Brassica napus) and 6 varieties from 5 cruciferous species was assessed by sexual compatibility through aniline blue fluorescence observation, manual cross, mentor pollina-tion with the mixture pollen and DNA identification. The results indicated that the compatibility indices of two B. napus varieties, B. juncea var. multiceps Tsen et Lee and Chinese cabbage (B. chinensis) (as female) with Q3 (as male) were 16.15, 12.77, 2.345 and 0.85, respectively. F1 plants were examined by PCR and all of these samples were confirmed as positive. Crossing between Orychophragmus violaceus, Des-curainia sophia (as female) and Q3 (as male) expressed high cross-incompatibility. Fluorescence microscope observation indicated that growing of pollen tube terminated at the upper 1/3 part of the style of O. violaceus and on the stigma surface of D. sophia. The mentor plants were further analyzed by PCR and all were shown to be negative. Under the neighboring growth and natural pollination conditions, the rates of gene flow to two B. napus varieties were 2.3278% and 2.1487%, respectively, B. juncea var. multiceps Tsen et Lee and Chinese cabbage were 1.0157% and 0.9236%, re-spectively. No gene flow was observed in O. violaceus and D. sophia. Frequency of gene flow was highly correlated with sexual compatibility. Due to possible alien gene flow among crucifers, special consideration and care should be taken to grow GM rapeseed.

关键词：除草剂十字花科植物荧光性遗传因子

Investigation of CRISPR/Cas9-induced SD1 rice mutants highlights the importance of molecular characterization in plant molecular breeding

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Journal of Genetics and Genomics 2020年第5期47卷 273-280页

作者： Sukumar Biswas Jiaqi Tian Rong Li Xiaofei Chen Zhijing Luo Mingjiao Chen xiangxiang zhao Dabing Zhang Staffan Persson Zheng Yuan Jianxin Shi Joint International Research Laboratory of Metabolic&Developmental Sciences Shanghai Jiao Tong University-University of Adelaide Joint Centre for Agriculture and HealthState Key Laboratory of Hybrid RiceSchool of Life Sciences and BiotechnologyShanghai Jiao Tong UniversityShanghai200240China Key Laboratory of Crop Marker-Assisted Breeding of Huaian Municipality Jiangsu Collaborative Innovation Center of Regional Modern Agriculture and Environmental ProtectionHuaian223300China School of Biosciences University of MelbourneParkville VIC3010MelbourneAustralia

Although Clustered Regularly Interspaced Short Palindromic Repeats(CRISPR)/CRISPR-associated 9(Cas9)system has been widely used for basic research in model plants,its application for applied breeding in crops has faced strong regulatory obstacles,due mainly to a poor understanding of the authentic output of this system,particularly in higher *** this study,different from any previous studies,we investigated in detail the molecular characteristics and production performance of CRISPR/Cas9-generated SD1(semi-dwarf 1)mutants from T2 to T4 generations,of which the selection of T1 and T2 was done only by visual phenotyping for semidwarf *** data revealed not only on-and off-target mutations with small or lager indels but also exogenous elements in T2 *** indel mutants passed stably to T3 or T4 without additional modifications independent on the presence of Cas9,while some lines displayed unexpected hereditary patterns of Cas9 or some exogenous *** addition,effects of various SD1 alleles on rice height and yield differed depending on genetic *** together,our data showed that the CRISPR/Cas9 system is effective in producing homozygous mutants for functional analysis,but it may be not as precise as expected in rice,and that early and accurate molecular characterization and screening must be carried out for generations before transitioning of the CRISPR/Cas9 system from laboratory to field.

关键词： CRISPR/Cas9 Plant height Oryza sativa Semi-dwarf 1 Yield

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代谢组学在谷物食品中的应用研究进展

食品工业科技 2024年第7期45卷 35-43页

作者：范琦琦赵香香吴鸣李祥张帅喻江刘晓飞张娜哈尔滨商业大学食品工程学院黑龙江省普通高等学校食品科学与工程重点实验室黑龙江省谷物食品与资源综合加工重点实验室黑龙江哈尔滨150076

谷物食品是人类每日能量、蛋白质和膳食纤维摄入的主要来源。随着人们生活水平的不断提升,谷物食品的营养价值、质量安全和食用品质备受关注。谷物代谢组学作为一个新兴且快速发展的“代谢组学”分支,在量化和分析各种代谢产物方面取得... 详细信息

谷物食品是人类每日能量、蛋白质和膳食纤维摄入的主要来源。随着人们生活水平的不断提升,谷物食品的营养价值、质量安全和食用品质备受关注。谷物代谢组学作为一个新兴且快速发展的“代谢组学”分支,在量化和分析各种代谢产物方面取得了快速发展。本文综述了代谢组学技术的分析流程,以及其在谷物食品成分鉴定、风味物质鉴定、产地溯源、新产品开发等方面的应用,并对代谢组学在谷物食品领域的发展趋势进行了展望,以期为谷物食品深度开发与应用提供参考。

关键词：代谢组学谷物食品风味检测食品溯源产品开发

OsMADS32 interacts with PI-like proteins and regulates rice flower development

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Journal of Integrative Plant Biology 2015年第5期57卷 504-513页

作者： Huanhuan Wang Liang Zhang Qiang Cai Yun Hu Zhenming Jin xiangxiang zhao Wei Fan Qianming Huang Zhijing Luo Mingjiao Chen Dabing Zhang Zheng Yuan State Key Laboratory of Hybrid Rice School of Life Sciences and BiotechnologyShanghai Jiao Tong University College of Life and Basic Sciences Sichuan Agricultural University Jiangsu Key Laboratory for Eco-Agricultural Biotechnology around Hongze Lake Huaiyin Normal University

OsMADS32 is a monocot specific MIKCc type MADS‐box gene that plays an important role in regulating rice floral meristem and organs identity, a crucial process for reproductive success and rice yield. However, its underlying mechanism of action remains to be clarified. Here, we characterized a hypomorphic mutant allele of OsMADS32/CFO1, cfo1‐3 and identified its function in controlling rice flower development by bioinformatics and protein‐protein interaction analysis. The cfo1‐3 mutant produces defective flowers, including loss of lodicule identity, formation of ectopic lodicule or hull‐like organs and decreased stamen number, mimicking phenotypes related to the mutation of B class genes. Molecular characterization indicated that mis‐splicing of OsMADS32 transcripts in the cfo1‐3 mutant resulted in an extra eight amino acids in the K‐domain of OsMADS32 protein. By yeast two hybrid and bimolecular fluorescence comple-mentation assays, we revealed that the insertion of eight amino acids or deletion of the internal region in the K1 subdomain of OsMADS32 affects the interaction between OsMADS32 with PISTILLATA （PI）‐like proteins OsMADS2 and OsMADS4. This work provides new insight into the mecha-nism by which OsMADS32 regulates rice lodicule and stamen identity, by interaction with two PI‐like proteins via its K domain.

关键词： Floral organ identity hypomorphic mutant K domain OsMADS32 protein interaction

Interactions of OsMADS1 with Floral Homeotic Genes in Rice Flower Development

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Molecular Plant 2015年第9期8卷 1366-1384页

作者： Yun Hu Wanqi Liang Changsong Yin Xuelian Yang Baozhe Ping Anxue Li Ru Jia Mingjiao Chen Zhijing Luo Qiang Cai xiangxiang zhao Dabing Zhang Zheng Yuan State Key Laboratory of Hybrid Rice Shanghai Jiao Tong University-University of Adelaide Joint Centre for Agriculture and Health School of Life Sciences andBiotechnology Shanghai Jiao Tong University Shanghai 20040 China Shanghai Ocean University Shanghai 201306 China jiangsu Collaborative Innovation Center of Regional Modern Agriculture and Environmental Protection Huaiyin Normal University Huaian 223300 China School of Agriculture Food and Wine University of Adelaide Waite Campus Urrbrae SA 5064 Australia

During reproductive development, rice plants develop unique flower organs which determine the final grain yield. OsMADS1, one of SEPALLATA-like MADS-box genes, has been unraveled to play critical roles in rice floral organ identity specification and floral meristem determinacy. However, the molecular mechanisms underlying interactions of OsMADS1 with other floral homeotic genes in regulating flower development remains largely elusive. In this work, we studied the genetic interactions of OsMADS1 with B-, C-, and D-class genes along with physical interactions among their proteins. We show that the physical and genetic interactions between OsMADS1 and OsMADS3 are essential for floral meristem activity maintenance and organ identity specification; while OsMADS1 physically and genetically interacts with OsMADS58 in regu- lating floral meristem determinacy and suppressing spikelet meristem reversion. We provided important genetic evidence to support the neofunctionalization of two rice C-class genes （OsMADS3 and OsMADS58） during flower development. Gene expression profiling and quantitative RT-PCR analyses further revealed that OsMADS1 affects the expression of many genes involved in floral identity and hormone signaling, and chromatin immunoprecipitation （ChlP）-PCR assay further demonstrated that OsMADS17 is a direct target gene of OsMADS1. Taken together, these results reveal that OsMADS1 has diversified regulatory functions in specifying rice floral organ and meristem identity, probably through its genetic and physical interactions with different floral homeotic regulators.

关键词： OsMADS1 floral homeotic genes floral organ identity floral meristem regulatory network

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植物乳杆菌WLPL04的荧光标记及其表征

南昌大学学报（理科版） 2024年第2期48卷 156-165页

作者：钟常城王祺安张启蒙赵香香王志跃魏华陶雪莹南昌大学食品科学与资源挖掘全国重点实验室江西南昌330047 南昌大学中德联合研究院江西南昌330047 南昌大学国际食品创新研究院有限公司江西南昌330299

植物乳杆菌WLPL04源自健康母乳,能在肠道定植。对植物乳杆菌WLPL04进行荧光标记,为研究其在体内的定植、移位及分布提供重要实验材料。以pMG36e为骨架,含3种不同启动子(P_(X),P_(23)和P_(ldh))的mCherry红色荧光蛋白基因作为标记基因,... 详细信息

植物乳杆菌WLPL04源自健康母乳,能在肠道定植。对植物乳杆菌WLPL04进行荧光标记,为研究其在体内的定植、移位及分布提供重要实验材料。以pMG36e为骨架,含3种不同启动子(P_(X),P_(23)和P_(ldh))的mCherry红色荧光蛋白基因作为标记基因,构建重组质粒pMG36e-P_(X)-mCherry、pMG36e-P_(23)-mCherry和pMG36e-P_(ldh)-mCherry,转化大肠杆菌,通过PCR、质粒DNA酶切、菌落颜色观察和激光共聚焦显微镜观察进行验证。验证构建成功后进一步将3种重组质粒分别导入植物乳杆菌WLPL04。结果显示导入质粒pMG36e-P_(X)-mCherry、pMG36e-P_(23)-mCherry的植物乳杆菌WLPL04,其菌体在激光共聚焦显微镜下均显示明显红色荧光;而导入pMG36e-P_(ldh)-mCherry的菌体其红色荧光较弱。此外,通过生长曲线和扫描电镜观察分析不同重组质粒对菌株生长及形态的影响,结果表明3种质粒对植物乳杆菌WLPL04的生长及菌体形态没有显著影响。以上结果表明植物乳杆菌WLPL04已被红色荧光蛋白mCherry成功标记,可为后续研究其在体内的定植、移位及分布提供重要实验材料。

关键词：植物乳杆菌WLPL04 mCherry荧光标记启动子表达

Integrating PCR-free amplification and synergistic sensing for ultrasensitive and rapid CRISPR/Cas12a-based SARS-CoV-2 antigen detection

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Synthetic and Systems Biotechnology 2021年第4期6卷 283-291页

作者： xiangxiang zhao Zhengduo Wang Bowen Yang Zilong Li Yaojun Tong Yuhai Bi Zhenghong Li Xuekui Xia Xiangyin Chen Lixin Zhang Weishan Wang Gao-Yi Tan State Key Laboratory of Bioreactor Engineering and School of BiotechnologyEast China University of Science and Technology(ECUST)Shanghai200237China State Key Laboratory of Microbial Resources Institute of MicrobiologyChinese Academy of SciencesBeijing100101China State Key Laboratory of Microbial Metabolism and School of Life Sciences&BiotechnologyShanghai Jiao Tong UniversityShanghai200240China CAS Key Laboratory of Pathogenic Microbiology and Immunology Institute of MicrobiologyCenter for Influenza Research and Early-warning(CASCIRE)CAS-TWAS Center of Excellence for Emerging Infectious Diseases(CEEID)Chinese Academy of SciencesBeijing100101China Key Biosensor Laboratory of Shandong Province Biology InstituteQilu University of Technology(Shandong Academy of Sciences)Jinan250013China

Antigen detection provides particularly valuable information for medical diagnoses;however,the current detection methods are less sensitive and accurate than nucleic acid *** combination of CRISPR/Cas12a and aptamers provides a new detection paradigm,but sensitive sensing and stable amplification in antigen detection remain ***,we present a PCR-free multiple trigger dsDNA tandem-based signal amplification strategy and a de novo designed dual aptamer synergistic sensing *** of these two strategies endowed the CRISPR/Cas12a and aptamer-based method with ultra-sensitive,fast,and stable antigen *** a demonstration of this method,the limit of detection was at the single virus level(0.17 fM,approximately two copies/μL)in SARS-CoV-2 antigen nucleocapsid protein analysis of saliva or serum *** entire procedure required only 20 *** our system’s simplicity and modular setup,we believe that it could be adapted reasonably easily for general applications in CRISPR/Cas12a-aptamer-based detection.

关键词： CRISPR/Cas12a Aptamer Antigen detection SARS-CoV-2 PCR-Free amplification Synergistic sensing