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脑卒中患者肺功能康复的研究进展

分子影像学杂志 2018年第4期41卷 546-549页

作者：罗文浩郑相涛赵一瑾南方医科大学第二临床医学院广东广州510282 南方医科大学珠江医院康复医学科广东广州510282

近年来,脑卒中的发病率逐年升高,医院内脑卒中患者人数也持续上涨。急性脑卒中患者的心肺活动能力受限,长期卧床导致排痰功能下降,易引起肺水肿和肺部感染等并发症,对脑卒中患者的转归与康复产生极大阻碍。本文从发病机制对脑卒中相关... 详细信息

近年来,脑卒中的发病率逐年升高,医院内脑卒中患者人数也持续上涨。急性脑卒中患者的心肺活动能力受限,长期卧床导致排痰功能下降,易引起肺水肿和肺部感染等并发症,对脑卒中患者的转归与康复产生极大阻碍。本文从发病机制对脑卒中相关性肺功能障碍进行了细致全貌分析,系统阐述了其康复治疗的难点,在此基础上优化了脑卒中患者肺功能康复的治疗方法,并对卒中后肺康复治疗的发展指明了方向。

关键词：肺功能康复治疗脑卒中

VGX-1027对小鼠心脏移植物缺血再灌注损伤的保护作用

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温州医科大学学报 2016年第3期46卷 183-186页

作者：戴驹骥郑祥韬傅红兴周蒙滔温州医科大学附属第一医院普外科浙江温州325015 温州医科大学药学院浙江温州325035

目的:探究VGX-1027对小鼠心脏移植物缺血再灌注损伤的保护作用。方法:取40只C57BL/6小鼠随机分为2组,分别为单纯心脏移植组和心脏移植+VGX-1027治疗组,每组各20只。移植术后24 h获取供心,通过HE染色及CD3、CD4和CD8免疫组织化学观察移... 详细信息

目的:探究VGX-1027对小鼠心脏移植物缺血再灌注损伤的保护作用。方法:取40只C57BL/6小鼠随机分为2组,分别为单纯心脏移植组和心脏移植+VGX-1027治疗组,每组各20只。移植术后24 h获取供心,通过HE染色及CD3、CD4和CD8免疫组织化学观察移植心脏的病理学改变;Western blot法检测移植术后供心caspase-3、Bax和Bcl-2蛋白的表达;ELISA法检测移植术后小鼠血清炎性因子TNF-α、IL-1β和IL-6的表达。结果:与单纯心脏移植组相比,心脏移植+VGX-1027治疗组炎症浸润明显减少,免疫组织化学显示CD3^+T细胞、CD4^+T细胞和CD8^+T细胞的浸润数量明显减少;Western blot法检测结果显示Bax和caspase-3的蛋白表达量显著减少(P<0.05),Bcl-2蛋白的表达量显著增加(P<0.05);ELISA法结果显示血清中TNF-α、IL-1β和IL-6的表达水平显著下降(P<0.05)。结论:VGX-1027可以通过抑制移植心脏的凋亡及炎性因子的释放减轻对心脏移植物缺血再灌注的损伤。

关键词：心脏移植缺血再灌注损伤 VGX-1027 C57BL/6小鼠

多普勒超声对下肢慢性静脉功能不全症患者中髂静脉压迫的预测价值

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中国现代医生 2020年第30期58卷 22-27页

作者：苏翔孙学余裘益辉杨法镜潘乐门郑祥韬温州医科大学附属第一医院血管外科浙江温州325000

目的探讨多普勒超声诊断髂静脉压迫综合征的价值。方法选取2017年10月~2019年9月我院治疗的下外周慢性静脉功能不全症患者402例,行多普勒超声和顺行静脉造影检查,以静脉造影结果为诊断标准,将402例患者分为髂静脉压迫组和对照组。分析... 详细信息

目的探讨多普勒超声诊断髂静脉压迫综合征的价值。方法选取2017年10月~2019年9月我院治疗的下外周慢性静脉功能不全症患者402例,行多普勒超声和顺行静脉造影检查,以静脉造影结果为诊断标准,将402例患者分为髂静脉压迫组和对照组。分析多普勒超声诊断髂静脉压迫综合征的价值。结果髂静脉压迫综合征患者的多普勒超声诊断总灵敏度为73.6%,特异度为93.7%。比较髂静脉压迫组与对照组的超声数据,结果显示几个指标可帮助诊断髂静脉压迫:左髂静脉最狭窄处与狭窄后血流峰速比值>2.000的灵敏度为92.13(84.50~96.80),特异度为95.83(93.0~97.8);右髂静脉与左髂静脉分叉处血流峰速比值>1.500的灵敏度为69.32(58.60~78.70),特异度为80.77(76.00~85.00);Valsalva动作后左股总静脉血流峰速变化比值<2的灵敏度为92.10%,特异度为84.80%,阳性似然比为6.06,阴性似然比为0.09。结论多普勒超声是从下肢静脉功能不全患者中筛选髂静脉压迫综合征患者的合理方法。

关键词：多普勒超声下肢静脉顺行造影髂静脉压迫综合征下肢静脉功能不全 May-Thurner综合征

Foot-and-Mouth Disease Virus Inhibits RIP2 Protein Expression to Promote Viral Replication

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Virologica Sinica 2021年第4期36卷 608-622页

作者： Huisheng Liu Qiao Xue Zixiang Zhu Fan Yang Weijun Cao xiangtao Liu Haixue zheng State Key Laboratory of Veterinary Etiological Biology National Foot and Mouth Diseases Reference LaboratoryKey Laboratory of Animal Virology of Ministry of AgricultureLanzhou Veterinary Research InstituteChinese Academy of Agricultural SciencesLanzhou 730046China

Receptors interaction protein 2(RIP2)is a specific adaptor molecule in the downstream of *** role of RIP2 during foot-and-mouth disease virus(FMDV)infection remains ***,our results showed that RIP2 inhibited FMDV replication and played an important role in the activation of IFN-βand NF-κB signal pathways during FMDV *** infection triggered RIP2 transcription,while it reduced the expression of RIP2 *** analysis showed that FMDV 2B,2C,3C^(pro),and L^(pro) proteins were responsible for inducing the reduction of RIP2 protein.3C^(pro) and L^(pro) are viral proteinases that can induce the cleavage or reduction of many host proteins and block host protein *** carboxyl terminal 105-C114 and 135-C144 regions of 2B were essential for reduction of *** results also showed that the N terminal 1-61 region of 2C were essential for the reduction of *** 2C-induced reduction of RIP2 was dependent on inducing the reduction of poly(A)-binding protein 1(PABPC1).The interaction between RIP2 and 2C was observed in the context of viral infection,and the residues 1-61 were required for the *** data clarify novel mechanisms of reduction of RIP2 mediated by FMDV.

关键词： Foot-and-mouth disease virus(FMDV) Receptors interaction protein 2(RIP2) PABPC1 2C Immune evasion

The Distribution of Different Clades of Seneca Valley Viruses in Guangdong Province,China

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Virologica Sinica 2018年第5期33卷 394-401页

作者： Pan Chen Fan Yang Weijun Cao Huanan Liu Keshan Zhang xiangtao Liu Zhiwen Xu Zixiang Zhu Haixue zheng State Key Laboratory of Veterinary Etiological Biology National Foot and Mouth Diseases Reference LaboratoryKey Laboratory of Animal Virology of Ministry of Agriculture Lanzhou Veterinary Research Institute Chinese Academy of Agricultural Sciences Animal Biotechnology Center Key Laboratory of Animal Disease and Human Health of Sichuan Province Sichuan Agricultural University

Seneca Valley virus (SVV), a newly determined etiological agent of vesicular disease in swine, causes porcine idiopathic disease and occasional acute death in piglets. Recently, an increased number of SVV infection cases have been reported in the United States (US) and China, resulting in significant economic losses to the swine industry. The first identification of SVV in China was reported in Guangdong Province, a major swine producing province. The cases of SVV were continuously reported in Guangdong in 2015 and 2016. However, the spread of SVV in Guangdong in 2017 remains *** this study, we determined two new SVV strains, CH-GD-2017-1 and CH-GD-2017-2, from Guangdong. The genetic analysis suggested that the two Guangdong strains showed different characteristics to previous Guangdong strains. They showed lower nucleotide similarity with strains isolated in 2015 and 2016, and were more similar to the US *** analyses indicated that the new strains were clustered in a different clade with previous Guangdong *** found 28 mutated amino acids in the new strains, compared with the first Guangdong strain, SVV CH-01-2015. In the geographic analysis, we found that the US and China reported more SVV cases than other countries, and most of the SVV cases were reported in east and central China—of which, Guangdong Province is one of the major epidemic regions. In conclusion, our findings indicate that SVV continued to spread in Guangdong Province in 2017, and two different clades of SVVs have emerged in this region.

关键词： Seneca Valley virus (SVV) Genomic sequence Phylogenetic tree Epidemiological analysis

FoxJ1 inhibits African swine fever virus replication and viral S273R protein decreases the expression of FoxJ1 to impair its antiviral effect

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Virologica Sinica 2022年第3期37卷 445-454页

作者： Caina Ma Shasha Li Fan Yang Weijun Cao Huisheng Liu Tao Feng Keshan Zhang Zixiang Zhu xiangtao Liu Yonghao Hu Haixue zheng College of Veterinary Medicine Gansu Agricultural UniversityLanzhou730070China State Key Laboratory of Veterinary Etiological Biology National Foot and Mouth Diseases Reference LaboratoryKey Laboratory of Animal Virology of Ministry of AgricultureLanzhou Veterinary Research InstituteChinese Academy of Agricultural SciencesLanzhou730046China

African swine fever(ASF)is a highly pathogenic swine infectious disease that affects domestic pigs and wild boar,which is caused by the African swine fever virus(ASFV).ASF has caused huge economic losses to the pig industry and seriously threatens global food security and livestock *** date,there is no safe and effective commercial vaccine against *** the underlying mechanisms of ASFV-host interplay is critical for developing effective vaccines and drugs against *** the present study,RNA-sequencing,RT-qPCR and Western blotting analysis revealed that the transcriptional and protein levels of the host factor FoxJ1 were significantly down-regulated in primary porcine alveolar macrophages(PAMs)infected by ***-qPCR analysis showed that overexpression of FoxJ1 upregulated the transcription of type I interferon and interferon stimulating genes(ISGs)induced by poly(dA:dT).FoxJ1 revealed a function to positively regulate innate immune response,therefore,suppressing the replication of *** addition,Western blotting analysis indicated that FoxJ1 degraded ASFV MGF505-2R and E165R proteins through autophagy ***,RT-qPCR and Western blotting analysis showed that ASFV S273R inhibited the expression of ***,we determined that FoxJ1 plays an antiviral role against ASFV replication,and ASFV protein impairs FoxJ1-mediated antiviral effect by degradation of *** findings provide new insights into the antiviral function of FoxJ1,which might help design antiviral drugs or vaccines against ASFV infection.

关键词： African swine fever virus(ASFV) FoxJ1 ISGs MGF505-2R E165R S273R

CO oxidation on the heterodinuclear tantalum-nickel monoxide carbonyl complex anions

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Chinese Chemical Letters 2021年第2期32卷 854-860页

作者： Jumei Zhang Ya Li Yan Bai Gang Li Dong Yang Huijun zheng Jinghan Zou xiangtao Kong Hongjun Fan Zhiling Liu Ling Jiang Hua Xie State Key Laboratory of Molecular Reaction Dynamics Collaborative Innovation Center of Chemistry for Energy and Materials(iChEM)Dalian Institute of Chemical PhysicsChinese Academy of SciencesDalian 116023China School of Chemical and Material Science Key Laboratory of Magnetic Molecules&Magnetic Information MaterialsMinistry of EducationShanxi Normal UniversityLinfen 041004China University of Chinese Academy of Sciences Beijing 100049China

The series of heterodinuclear metal oxide carbonyls in the form of TaNiO(CO)_(n)(n=5-8) are generated in the pulsed-laser vaporization source and characterized by mass-selected photoelectron velocity-map *** the consecutive CO adsorption,the μ^(2)-O-bent structure initially is the most favorable for TaNiO(CO)_(5),and subsequently both μ^(2)-O-bent and μ^(2)-O-linear structures are degenerate for TaNiO(CO)_(6),then the μ^(2)-O-linear structure is most preferential for TaNiO(CO)_(7),and finally the η^(2)-CO_(2)-tagged structure is the most ene rgetically competitive one for TaNiO(CO)_(8),i.e., the CO oxidation occurs at n=*** contrast to the literature reported CO oxidation on heteronuclear metal oxide complexes generally proceeding via Langmuir-Hinshelwood-like mechanism,complementary theo retical calculations suggest that both Langmuir-Hinshelwood-like and Eley-Rideal-like mechanisms prevail for the CO oxidation reaction on TaNiO(CO)_(8) *** findings provide new insight into the composition-selective mechanism of CO oxidation on heteronuclear metal complexes,of which the composition be tailored to fulfill the desired chemical behaviors.

关键词： CO oxidation Photoelectron imaging Heteronuclear oxide Density functional theory Transition metal carbonyl

Porcine Picornavirus 3C Protease Degrades PRDX6 to Impair PRDX6-mediated Antiviral Function

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Virologica Sinica 2021年第6期36卷 948-957页

作者： Congcong Wang Huanhuan Feng Xiangle Zhang Kangli Li Fan Yang Weijun Cao Huisheng Liu Lili Gao Zhaoning Xue xiangtao Liu Zixiang Zhu Haixue zheng State Key Laboratory of Veterinary Etiological Biology OIE/National Foot and Mouth Diseases Reference LaboratoryKey Laboratory of Animal Virology of Ministry of AgricultureLanzhou Veterinary Research InstituteChinese Academy of Agricultural SciencesLanzhou 730046China

Peroxiredoxin-6(PRDX6)is an antioxidant enzyme with both the activities of peroxidase and phospholipase A2(PLA2),which is involved in regulation of many cellular ***,the function of PRDX6 during virus infection remains *** this study,we found that the abundance of PRDX6 protein was dramatically decreased in foot-and-mouth disease virus(FMDV)infected *** of PRDX6 inhibited FMDV *** contrast,knockdown of PRDX6 expression promoted FMDV replication,suggesting an antiviral role of *** explore whether the activity of peroxidase and PLA2 was associated with PRDX6-mediated antiviral function,a specific inhibitor of PLA2(MJ33)and a specific inhibitor of peroxidase activity(mercaptosuccinate)were used to treat the cells before FMDV *** results showed that incubation of MJ33 but not mercaptosuccinate promoted FMDV ***,overexpression of PRDX6 slightly enhanced type I interferon *** further determined that the viral 3Cprowas responsible for degradation of PRDX6,and 3Cpro-induced reduction of PRDX6 was independent of the proteasome,lysosome,and caspase *** protease activity of 3Cprowas required for induction of PRDX6 ***,PRDX6 suppressed the replication of another porcine picornavirus Senecavirus A(SVA),and the 3Cproof SVA induced the reduction of PRDX6 through its proteolytic activity as ***,our results suggested that PRDX6 plays an important antiviral role during porcine picornavirus infection,and the viral 3Cproinduces the degradation of PRDX6 to overcome PRDX6-mediated antiviral function.

关键词： Porcine picornavirus Peroxiredoxin-6(PRDX6) 3Cpro Phospholipase A2(PLA2) Antagonism

Clinical and Experimental Study of Low Molecular Weight Heparin in Patients with Chronic Anemia

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International Journal of Clinical Medicine 2021年第9期12卷 357-363页

作者： Jiamin zheng Hong Shao Min Yan Ye Lu xiangtao Pan Department of Hematology Taicang Hospital of Soochow University Taicang China

Objective: To preliminary study the significance of low molecular weight heparin (LMWH) in the treatment patients of with anemia of chronic diseases (ACD), and the changes in the serum levels of BMP6, hepcidin and IL-6. To preliminary study the significance of low molecular weight heparin (LMWH) in the treatment the patients with anemia of chronic diseases (ACD), and the changes in the serum levels of BMP6, hepcidin and IL-6. Methods: Used LMWH (4000 u/day, 7 - 15 days) to therapy 61 patients with ACD, and ELISA method was used to determine Hepcidin and BMP6 before and after treatment, and the determination of IL-6 by Electro-chemi-luminescence, and to analyze its clinical significance. Results: 1) In all 61 cases, the levels of Hepcidin in post-therapy were 0.82 ± 0.24 mg/L, which were lower than 1.05 ± 3.83 mg/L in pre-therapy (t = 2.5726, P P P > 0.05). However, The levels of Hb in post-therapy were higher than in pre-therapy (t = 1.9832, P P P P P > 0.05). 5) Although Hb level inc

关键词： Anemia of Chronic Disease BMP6 Hepcidin IL-6 Hemoglobin Low Molecular Weight Heparin Therapy