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养殖水域Cu^(2+)胁迫对虹鳟MT2基因表达模式的影响

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环境科学与技术 2024年第2期47卷 96-103页

作者：周静卢军浩赵映灿徐永辉李湘茹巫佳俊权金强甘肃省生态环境科学设计研究院甘肃兰州730020 甘肃农业大学动物科学技术学院甘肃兰州730070

为了探究水体中铜离子胁迫对虹鳟(Oncorhynchus mykiss)MT2基因表达模式的影响,开发Metallothioneins作为检测水域生态中重金属铜污染的分子标志,该研究利用TA克隆法获得了虹鳟MT2基因的CDS序列,检测了MT2基因在虹鳟不同组织中的表达模... 详细信息

为了探究水体中铜离子胁迫对虹鳟(Oncorhynchus mykiss)MT2基因表达模式的影响,开发Metallothioneins作为检测水域生态中重金属铜污染的分子标志,该研究利用TA克隆法获得了虹鳟MT2基因的CDS序列,检测了MT2基因在虹鳟不同组织中的表达模式,以及Cu^(2+)胁迫(Cu^(2+)浓度75、150和300μg/L)后1、2、3和4 d和胁迫恢复1、2 d时间点的MT2基因表达动态。结果表明,虹鳟MT2基因CDS区长186 bp,编码62个氨基酸,具有典型的半胱氨酸(Cys)-X(1~3个)-半胱氨酸结构,属不稳定亲水性蛋白。虹鳟MT2基因序列与大西洋鲑的同源性最高且在肝脏中表达量最高,其次是脑和肠道。胁迫试验结果显示,MT2基因在肝脏和鳃中呈现先升高后降低的趋势,在头肾、脾脏和脑中呈现逐渐升高的趋势,且呈现一定的剂量依赖性,肠道MT2基因在胁迫2、3和4 d被显著抑制。胁迫恢复后,处理组肝脏、头肾、鳃、脑MT2基因表达量仍显著升高。综上,虹鳟MT2基因能在不同组织中被Cu^(2+)诱导表达,推测MT2基因在虹鳟抗重金属毒性中可能发挥着重要作用,且MT2基因表达量与铜离子的浓度相关,可以作为分子生物标志检测水域生态中的重金属铜污染。

关键词：虹鳟 MT2基因铜离子胁迫基因克隆表达模式

冷却速率对ZG40CrNi2Mo大型铸件凝固组织及元素偏析的影响

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热加工工艺 2024年第15期53卷 135-140页

作者：邹富康仲红刚李莉娟龚永勇陈湘茹翟启杰上海大学先进凝固技术中心上海200444

为了研究铸件凝固过程中不同冷却速率对其凝固组织与元素偏析的影响,利用铸件凝固过程数值模拟方法和自研的高通量凝固过程热模拟实验装置,开展了ZG40CrNi2Mo优质调质钢在不同冷却速率下的凝固模拟实验。结果表明:ZG40CrNi2Mo调质钢的... 详细信息

为了研究铸件凝固过程中不同冷却速率对其凝固组织与元素偏析的影响,利用铸件凝固过程数值模拟方法和自研的高通量凝固过程热模拟实验装置,开展了ZG40CrNi2Mo优质调质钢在不同冷却速率下的凝固模拟实验。结果表明:ZG40CrNi2Mo调质钢的二次枝晶臂间距λ_(2)与局部凝固时间t的关系符合λ_(2)=36.03 t0.26;随着冷却速率的降低,Cr元素偏析呈现上升趋势,Ni元素偏析先上升后降低;Mo元素偏析最为严重,但是对于冷却速率的变化不敏感。

关键词：热模拟数值模拟冷却速率凝固组织二次枝晶臂间距元素偏析

BC@CdS复合材料的原位生长制备及光催化性能

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精细化工 2024年第10期41卷 2189-2198,2205页

作者：谭岑黄相如陈明英苏亚斌陆星羽陈丛瑾广西大学化学化工学院广西石化资源加工及过程强化技术重点实验室广西南宁530004

以生物质气化炭渣为原料,采用原位水热生长策略制备了一系列生物炭(BC)负载CdS复合材料(BC@Cd S),通过XRD、SEM、BET、XPS、UV-Vis DRS、PL、EIS对其进行了表征。以左氧氟沙星(LVF)为目标污染物,探讨了m(BC)∶m(CdS)对BC@CdS光催化性能... 详细信息

以生物质气化炭渣为原料,采用原位水热生长策略制备了一系列生物炭(BC)负载CdS复合材料(BC@Cd S),通过XRD、SEM、BET、XPS、UV-Vis DRS、PL、EIS对其进行了表征。以左氧氟沙星(LVF)为目标污染物,探讨了m(BC)∶m(CdS)对BC@CdS光催化性能的影响,对影响BC@CdS光催化降解LVF的因素进行了考察,测试了其循环稳定性和对不同污染物的适用性,推测了BC@CdS光催化降解LVF的机理。结果表明,CdS纳米颗粒均匀生长在BC表面上,有效防止了自身的团聚;以m(BC)∶m(CdS)=1∶2制备的BC@CdS-2具有最优光催化性能,在90 min可见光照射下,20 mg BC@CdS-2对50 mL质量浓度为20 mg/L LVF进行降解,LVF降解率为90.87%,循环使用5次,LVF降解率仍可达85.45%;BC@CdS-2对质量浓度为20mg/L的不同污染物(环丙沙星、氧氟沙星、土霉素、盐酸四环素、罗明丹B)降解率在83.57%~93.65%之间;对体系环境的pH和共存离子有抗干扰能力。BC@CdS光催化体系中的主要活性基团是空穴和超氧自由基。

关键词：生物炭 BC@CdS 复合材料光催化降解左氧氟沙星功能材料

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鸡圆环病毒SYBR GreenⅠ实时荧光定量PCR检测方法的建立

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中国家禽 2024年第1期46卷 56-61页

作者：陈佳圣赵天琪刘东华孙祥茹陈文德李根青岛农业大学动物医学院山东青岛266109

为快速准确检测鸡圆环病毒(CCV),试验根据CCV Rep基因设计特异性引物,以CCV阳性病料提取的DNA为模板进行PCR扩增,构建CCV重组质粒,建立检测CCV的SYBR GreenⅠ实时荧光定量PCR方法。结果显示:试验建立的方法对2.87×10^(8)~2.87×10^(1)c... 详细信息

为快速准确检测鸡圆环病毒(CCV),试验根据CCV Rep基因设计特异性引物,以CCV阳性病料提取的DNA为模板进行PCR扩增,构建CCV重组质粒,建立检测CCV的SYBR GreenⅠ实时荧光定量PCR方法。结果显示:试验建立的方法对2.87×10^(8)~2.87×10^(1)copies/μL浓度范围的CCV重组质粒标准品呈现良好的线性关系,相关系数(R^(2))为0.9936,灵敏度比常规PCR高100倍;该方法对鸡传染性贫血病毒、新城疫病毒、传染性支气管炎病毒等常见鸡病毒性病原以及多杀性巴氏杆菌、禽致病性大肠杆菌、鸡白痢沙门氏菌无特异性扩增,批内和批间变异系数均不超过1%;该方法对60份疑似CCV感染临床样品检测结果显示,CCV阳性检出率(16.67%)高于常规PCR。上述结果表明,研究建立的CCV SYBR GreenⅠ实时荧光定量PCR方法具有良好的敏感性、特异性和重复性,检出限为2.87×10^(1)copies/μL,可以用于CCV的快速定量检测。

关键词：鸡圆环病毒 SYBR GreenⅠ实时荧光定量PCR 检测方法

钢筋混凝土夹板墙加固石砌体片墙力学性能分析

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湖南城市学院学报（自然科学版） 2024年第1期33卷 1-5页

作者：杨相如林婷婷巫生平福建船政交通职业学院土木工程学院福州350108 福建江夏学院工程学院福州350108

为保护福州市平潭地区传统石头厝建筑的独特风格,本研究拟采用钢筋混凝土夹板墙对其进行加固并对其力学性能加以分析。为明确该加固技术对内、外墙的不同加固效果,首先,利用有限元数值分析软件ABAQUS分别建立了未加固、单侧加固和双侧... 详细信息

为保护福州市平潭地区传统石头厝建筑的独特风格,本研究拟采用钢筋混凝土夹板墙对其进行加固并对其力学性能加以分析。为明确该加固技术对内、外墙的不同加固效果,首先,利用有限元数值分析软件ABAQUS分别建立了未加固、单侧加固和双侧加固的石砌体片墙试件的数值分析模型;然后,通过面外单向加载分析了混凝土加固层厚度及配筋率对加固墙体性的影响。结果表明,钢筋混凝土夹板墙能大幅度提高石砌体片墙的承载力,且随着加固层混凝土厚度的增加,墙体承载能力不断增大,但配筋率对加固效果的提高作用不明显。

关键词：石结构板墙加固平面外力学性能石头厝有限元

Effect of cooling rate on solidification structure and linear contraction of a duplex stainless steel

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China Foundry 2012年第3期9卷 239-243页

作者： Zhong Honggang Chen xiangru Ao Lu Zhai Qijie Shanghai Key Laboratory of Modern Metallurgy&Materials Processing Shanghai UniversityShanghai 200072China

Cooling rate is a key factor that can drastically affect the phase transformation and thermal stress of duplex stainless steels. Therefore, in this research, different sand moulds were used to explore the influence of cooling rate on the solidification of the 2304 duplex stainless steel (DSS). The macro and micro structures of the 2304 DSS were investigated. Small equiaxed grains are obtained in chromite sand mould sample with a lower pouring temperature and a higher cooling rate, whereas coarse columnar and equiaxed grains are found in silica sand and refractory powder mould samples. The size of austenite phase is significantly increased with decreasing cooling rate, while the ferrite phase content ranging from 51.6% to 53.9% does not change obviously. In addition, the linear contraction of the 2304 DSS decreases from 2.34% to 1.09% when the mean cooling rate above 1,173 K increases from 0.99 K·s-1 to 3.66 K·s-1.

关键词： duplex stainless steel solidification structure linear contraction cooling rate

G9a/GLP-sensitivity of H3K9me2 Demarcates Two Types of Genomic Compartments

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Genomics, Proteomics & Bioinformatics 2020年第4期18卷 359-370页

作者： Zixiang Yan Luzhang Ji xiangru Huo Qianfeng Wang Yuwen Zhang Bo Wen MOE Key Laboratory of Metabolism and Molecular Medicine Department of Biochemistry and Molecular BiologySchool of Basic Medical Sciencesand Institutes of Biomedical SciencesFudan UniversityShanghai 200032China State Key Laboratory of Genetic Engineering Collaborative Innovation Center of Genetics and DevelopmentFudan UniversityShanghai 200438China

In the nucleus, chromatin is folded into hierarchical architecture that is tightly linked to various nuclear functions. However, the underlying molecular mechanisms that confer these architectures remain incompletely understood. Here, we investigated the functional roles of H3 lysine 9 dimethylation(H3 K9 me2), one of the abundant histone modifications, in three-dimensional(3 D)genome organization. Unlike in mouse embryonic stem cells, inhibition of methyltransferases G9 a and GLP in differentiated cells eliminated H3 K9 me2 predominantly at A-type(active) genomic compartments, and the level of residual H3 K9 me2 modifications was strongly associated with B-type(inactive) genomic compartments. Furthermore, chemical inhibition of G9 a/GLP in mouse hepatocytes led to decreased chromatin-nuclear lamina interactions mainly at G9 a/GLP-sensitive regions, increased degree of genomic compartmentalization, and up-regulation of hundreds of genes that were associated with alterations of the 3 D chromatin. Collectively, our data demonstrated essential roles of H3 K9 me2 in 3 D genome organization.

关键词： H3K9me2 G9a/GLP Chromatin organization 3D genome Genomic compartment

Poly(L-glutamic acid)-cisplatin nanoformulations with detachable PEGylation for prolonged circulation half-life and enhanced cell internalization

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Bioactive Materials 2021年第9期6卷 2688-2697页

作者： Zhongyu Jiang xiangru Feng Haoyang Zou Weiguo Xu Xiuli Zhuang Key Laboratory of Polymer Ecomaterials Changchun Institute of Applied ChemistryChinese Academy of Sciences5625 Renmin StreetChangchun130022P.R.China School of Applied Chemistry and Engineering University of Science and Technology of China96 Jinzhai RoadHefei230026P.R.China

PEGylation has been widely applied to prolong the circulation times of nanomedicines via the steric shielding effect,which consequently improves the intratumoral ***,cell uptake of PEGylated nanoformulations is always blocked by the steric repulsion of PEG,which limits their therapeutic *** this end,we designed and prepared two kinds of poly(L-glutamic acid)-cisplatin(PLG-CDDP)nanoformulations with detachable PEG,which is responsive to specific tumor tissue microenvironments for prolonged circulation time and enhanced cell *** extracellular pH(pHe)-responsive cleavage 2-propionic-3-methylmaleic anhydride(CDM)-derived amide bond and matrix metalloproteinases-2/9(MMP-2/9)-sensitive degradable peptide PLGLAG were utilized to link PLG and PEG,yielding pHe-responsive PEG-pHe-PLG and MMP-sensitive *** corresponding smart nanoformulations PEG-pHe-PLG-Pt and PEG-MMP-PLG-Pt were then prepared by the complexation of polypeptides and cisplatin(CDDP).The circulation half-lives of PEG-pHe-PLG-Pt and PEG-MMP-PLG-Pt were about 4.6 and 4.2 times higher than that of the control PLG-Pt,*** reaching tumor tissue,PEG on the surface of nanomedicines was detached as triggered by pHe or MMP,which increased intratumoral CDDP retention,enhanced cell uptake,and improved antitumor efficacy toward a fatal high-grade serous ovarian cancer(HGSOC)mouse model,indicating the promising prospects for clinical application of detachable PEGylated nanoformulations.

关键词： Poly(L-glutamic acid) Detachable PEGylation Prolonged circulation time Enhanced cell uptake Platinum chemotherapy

Effects of Ultrasonic Seed Treatment on Rice Performances under the Seawater Irrigation

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Phyton-International Journal of Experimental Botany 2023年第1期92卷 121-130页

作者： Yingying Zhang Jinhai Liu Zhuosheng Yan Gangshun Rao xiangru Tang State Key Laboratory for Conservation and Utilization of Subtropical Agricultural Bioresources South China Agricultural UniversityGuangzhou510642China Scientific Observing and Experimental Station of Crop Cultivation in South China Ministry of AgricultureGuangzhou510642China Guangzhou Key Laboratory for Science and Technology of Aromatic Rice Guangzhou510642China Golden Rice Agricultural Science&Technology Co. Ltd.Guangzhou510642China College of Coastal Agricultural Sciences Guangdong Ocean UniversityZhanjiang524088China

Irrigation with desalinated seawater is an effective way to use ocean resources and save freshwater ***,seawater irrigation would cause yield loss of *** order to explore the effects of ultrasonic seed treatment on rice performances under seawater irrigation,the present study was conducted with three irrigation treatments(fresh water(SW0),ten times diluted seawater(SW1%,0.34%salinity),and five times diluted seawater(SW2%,0.68%salinity))and two seed treatments(ultrasonic treated seeds(UT)and untreated seeds(CK)).Compared with SW0+CK treatment,SW1+CK and SW2+CK treatments significantly decreased grain yield by 56.19%and 66.69%,spikelets per panicle by 30.11%and 55.80%,seed-setting rate by 23.05%and 18.87%,and 1000-grain weight by 4.55%and 14.50%,*** irrigation also significantly increased malonaldehyde(MDA)and proline contents and the activities of superoxide dismutase(SOD)and peroxidase(POD).Ultrasonic seed treatment significantly increased the grain number per panicle,seed-setting rate,and grain yield of rice under seawater *** with CK,UT treatment substantially reduced MDA content,SOD activity,and POD activity in SW1 and SW2 ***,UT treatment significantly increased proline content and down-regulated proline dehydrogenase activity under seawater *** deduced that ultrasonic seed treatment enhanced the salinity tolerance of rice by inducing the proline *** findings indicated that ultrasonic seed treatment could an effective strategy to promote rice productivity under seawater irrigation.

关键词： Proline rice seawater irrigation ultrasonic waves yield formation