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Genetic analysis implicates a molecular chaperone complex in regulating epigenetic silencing of methylated genomic regions

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Journal of Integrative Plant Biology 2021年第8期63卷 1451-1461页

作者： Zhengyan Feng Xiangqiang Zhan Jia Pang Xue Liu Huiming zhang Zhaobo Lang Jian-Kang Zhu Shanghai Center for Plant Stress Biology and Center for Excellence in Molecular Plant Sciences Chinese Academy of SciencesShanghai201602 China State Key Laboratory of Crop Stress Biology for Arid Areas and College of Horticulture Northwest A&F UniversityYangling712100 China University of Chinese Academy of Sciences Beijing100049 China

DNA cytosine methylation confers stable epigenetic silencing in plants and many animals.However,the mechanisms underlying DNA methylation-mediated genomic silencing are not fully understood.We conducted a forward genetic screen for cellular factors required for the silencing of a heavily methylated p35S:NPTII transgene in the Arabidopsis thaliana rdm1-1 mutant background,which led to the identification of a Hsp20 family protein,RDS1(rdm1-1 suppressor 1).Loss-of-function mutations in RDS1 released the silencing of the p35S::NPTII transgene in rdm1-1 mutant plants,without changing the DNA methylation state of the transgene.Protein interaction analyses suggest that RDS1 exists in a protein complex consisting of the methyl-DNA binding domain proteins MBD5 and MBD6,two other Hsp20 family proteins,RDS2 and IDM3,a Hsp40/DNAJ family protein,and a Hsp70 family protein.Like rds1 mutations,mutations in RDS2,MBD5,or MBD6 release the silencing of the transgene in the rdm1 mutant background.Our results suggest that Hsp20,Hsp40,and Hsp70 proteins may form a complex that is recruited to some genomic regions with DNA methylation by methyl-DNA binding proteins to regulate the state of silencing of these regions.

关键词： epigenetic silencing heat shock proteins molecular chaperone complex

The GAMYB-like gene SlMYB33 mediates flowering and pollen development in tomato

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Horticulture Research 2020年第1期7卷 903-918页

作者： Yan zhang Bo zhang Tongwen Yang Jie zhang Bin Liu Xiangqiang Zhan Yan Liang College of Horticulture Northwest A&F UniversityYangling 712100 ShaanxiP.R.China School of Agriculture and Biology Shanghai Jiao Tong UniversityShanghai 200240China

GAMYBs are positive GA signaling factors that exhibit essential functions in reproductive development,particularly in anther and pollen development.However,there is no direct evidence of the regulation of any GAMYB in these biological processes in tomato(Solanum lycopersicum).Here,we identified a tomato GAMYB-like gene,SlMYB33,and characterized its specific roles.SlMYB33 is predominately expressed in the stamens and pistils.During flower development,high mRNA abundance of SlMYB33 is detected in both male and female organs,such as microspore mother cells,anthers,pollen grains,and ovules.Silencing of SlMYB33 leads to delayed flowering,aberrant pollen viability,and poor fertility in tomato.Histological analyses indicate that SlMYB33 exerts its function in pollen development in the mature stage.Further transcriptomic analyses imply that the knockdown of SlMYB33 significantly inhibits the expression of genes related to flowering in shoot apices,and alters the transcription of genes controlling sugar metabolism in anthers.Taken together,our study suggests that SlMYB33 regulates tomato flowering and pollen maturity,probably by modulating the expression of genes responsible for flowering and sugar metabolism,respectively.

关键词： metabolism sugar media

4-methylumbelliferone (4-MU) enhances drought tolerance of apple by regulating rhizosphere microbial diversity and root architecture

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Horticulture Research 2023年第7期10卷 53-68页

作者： Dehui zhang Jieqiang He Pengda Cheng Yutian zhang Abid Khan Shicong Wang Zhongxing Li Shuang Zhao Xiangqiang Zhan Fengwang Ma Xuewei Li Qingmei Guan State Key Laboratory of Crop Stress Biology for Arid Areas/Shaanxi Key Laboratory of Apple College of HorticultureNorthwest A&F UniversityYangling 712100China College of Life Science Northwest A&F UniversityYangling 712100China Department of Horticulture The University of HaripurHaripur 22620Pakistan

The dwarfing rootstocks-mediated high-density apple orchard is becoming the main practice management.Currently,dwarfing rootstocks are widely used worldwide,but their shallow root system and drought sensitivity necessitate high irrigation requirements.Here,the root transcriptome and metabolome of dwarfing(M9-T337,a drought-sensitive rootstock)and vigorous rootstocks(Malus sieversii,a drought-tolerant species,is commonly used as a rootstock)showed that a coumarin derivative,4-Methylumbelliferon(4-MU),was found to accumulate significantly in the roots of vigorous rootstock under drought condition.When exogenous 4-MU was applied to the roots of dwarfing rootstock under drought treatment,the plants displayed increased root biomass,higher root-to-shoot ratio,greater photosynthesis,and elevated water use efficiency.In addition,diversity and structure analysis of the rhizosphere soil microbial community demonstrated that 4-MU treatment increased the relative abundance of putatively beneficial bacteria and fungi.Of these,Pseudomonas,Bacillus,Streptomyces,and Chryseolinea bacterial strains and Acremonium,Trichoderma,and Phoma fungal strains known for root growth,or systemic resistance against drought stress,were significantly accumulated in the roots of dwarfing rootstock after 4-MU treatment under drought stress condition.Taken together,we identified a promising compound—4-MU,as a useful tool,to strengthen the drought tolerance of apple dwarfing rootstock.

关键词： drought dwarf roots

The tomato 2-oxoglutarate-dependent dioxygenase gene SlF3HL is critical for chilling stress tolerance

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Horticulture Research 2019年第1期6卷 1153-1164页

作者： Tixu Hu Yuqin Wang Qiqi Wang Ningning Dang Ling Wang Chaochao Liu Jianhua Zhu Xiangqiang Zhan State Key Laboratory of Crop Stress Biology for Arid Areas and College of Horticulture Northwest A&F UniversityNo.3Taicheng Road712100 YanglingShaanxiChina School of Biotechnology Jiangsu University of Science and TechnologyZhenjiangJiangsuChina Department of Plant Science and Landscape Architecture College of Agriculture and Natural ResourcesUniversity of MarylandCollege ParkMD 20742USA

Low temperature is a major stress that severely affects plant development,growth,distribution,and productivity.Here,we examined the function of a 2-oxoglutarate-dependent dioxygenase-encoding gene,SlF3HL,in chilling stress responses in tomato(Solanum lycopersicum cv.Alisa Craig[AC]).Knockdown(KD)of SlF3HL(through RNA interference)in tomato led to increased sensitivity to chilling stress as indicated by elevated levels of electrolyte leakage,malondialdehyde(MDA)and reactive oxygen species(ROS).In addition,the KD plants had decreased levels of proline and decreased activities of peroxisome and superoxide dismutase.The expression of four cold-responsive genes was substantially reduced in the KD plants.Furthermore,seedling growth was significantly greater in AC or SlF3HLoverexpression plants than in the KD plants under either normal growth conditions with methyl jasmonate(MeJA)or chilling stress conditions.SlF3HL appears to positively regulate JA accumulation and the expression of JA biosynthetic and signaling genes under chilling stress.Together,these results suggest that SlF3HL is a positive regulator of chilling stress tolerance and functions in the chilling stress tolerance pathways,possibly by regulating JA biosynthesis,JA signaling,and ROS levels.

关键词： elevated stress chilling

A universal bifurcation mechanism arising from progressive hydroelastic waves

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Theoretical & Applied Mechanics Letters 2022年第1期12卷 23-29页

作者： zhan Wang Institute of Mechanics Chinese Academy of SciencesBeijing 100190China School of Engineering Science University of Chinese Academy of SciencesBeijing 100049China

A unidirectional, weakly dispersive nonlinear model is proposed to describe the supercritical bifurcation arising from hydroelastic waves in deep water. This model equation, including quadratic, cubic, and quartic nonlinearities, is an extension of the famous Whitham equation. The coefficients of the nonlinear terms are chosen to match with the key properties of the full Euler equations, precisely, the associated cubic nonlinear Schrödinger equation and the amplitude of the solitary wave at the bifurcation point. It is shown that the supercritical bifurcation, rich with Stokes, solitary, generalized solitary, and dark solitary waves in the vicinity of the phase speed minimum, is a universal bifurcation mechanism. The newly developed model can capture the essential features near the bifurcation point and easily be generalized to other nonlinear wave problems in hydrodynamics.

关键词： Nonlinear wave Supercritical bifurcation Hydroelastic wave Wavepacket

Impacts of human activities on morphological evolution in the Modaomen Estuary, China

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Acta Oceanologica Sinica 2023年第5期42卷 79-92页

作者： Tiehan Liao Haigang zhan Xing Wei Weikang zhan State Key Laboratory of Tropical Oceanography South China Sea Institute of OceanologyChinese Academy of SciencesGuangzhou 510301China University of Chinese Academy of Sciences Beijing 100049China Southern Marine Science and Engineering Guangdong Laboratory(Guangzhou) Guangzhou 511458China

The morphology of the Modaomen Estuary(ME)has undergone drastic changes in recent decades,and quantifying the contribution of human activities and natural processes is crucial for estuary management.Using Landsat images,chart data,and hydrological and meteorological data,this study analyzed the evolution of the shoreline and subaqueous topography of the ME and attempted to quantify the extent of the contributions of human activities.The results show that local human activities dominated morphological evolution in some periods.From 1973 to 2003,the shoreline advanced rapidly seaward,resulting in approximately half of the water area being converted into land.Human activity is critical to this process,with the direct contribution of local land reclamation projects reaching more than 85%.After 2003,the shoreline remained relatively stable,probably due to a decrease in land reclamation projects.Regarding the evolution of subaqueous topography,the shoals in the estuary were heavily silted and gradually disappeared during 1983–2003,and the waterways narrowed and deepened.The average siltation rate decreased from 15.43 mm/a to-1.02 mm/a,indicating that the ME changed from sedimentation to slight erosion.By detecting variations of sediment load,we found that upstream human activities reduced river sediment,while downstream human activities significantly increased sediment input to the ME,leaving little change in the actual sediment input to the ME for a relatively long period.In addition,based on the empirical relationship between the sediment input and siltation rate,local human activities influenced the shift in the siltation state more than upstream and downstream human activities did.These findings suggest that more attention should be paid to local human activities to improve the estuarine management in the ME.

关键词： Modaomen Estuary morphological evolution human activity quantitative analysis

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番茄花序梗部长度的遗传分析

中国蔬菜 2023年第9期 38-47页

作者：李艳琪曹晓宇李博宇王亦希张德楷战祥强胡体旭西北农林科技大学园艺学院陕西杨凌712100

为明确番茄花序梗部长度的遗传规律,以花序梗部长度较短的栽培品种Ailsa Craig(AC)和花序梗部长度较长的类番茄茄LA1964为亲本,建立P1、P2、F1、F24个世代群体,利用主基因+多基因遗传体系,分别对番茄第2、3、4花序的梗部长度进行遗传分... 详细信息

为明确番茄花序梗部长度的遗传规律,以花序梗部长度较短的栽培品种Ailsa Craig(AC)和花序梗部长度较长的类番茄茄LA1964为亲本,建立P1、P2、F1、F24个世代群体,利用主基因+多基因遗传体系,分别对番茄第2、3、4花序的梗部长度进行遗传分析。结果显示:第2花序和第3花序的总梗长、前梗长、后梗长均符合MX2-ADI-AD模型,第4花序总梗长、前梗长、后梗长的最适遗传模型为MX2-ADI-ADI,说明番茄花序梗部长度均由2对加性-显性-上位性主基因和多基因共同控制,初步判断多基因的上位性效应因温度变化而存在差异,主基因遗传率为46.61%~77.08%,多基因遗传率均为0,表明主基因遗传在番茄花序梗部长度的遗传中起主导作用。

关键词：番茄花序梗长主基因+多基因遗传分析

Polymerized A-DA'D-A type small-molecule acceptors for high performance all-polymer solar cells: progress and perspective

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中国科学:化学英文版 2023年第9期66卷 2513-2531页

作者： Ting Yang Chuanlang zhan Key Laboratory of Advanced Materials Chemistry and Devices(AMC&DLab)of the Department of Education of Inner Mongolia Autonomous Region College of Chemistry and Environmental ScienceInner Mongolia Normal UniversityHuhhot 010022China

A-DA'D-A type polymerized small-molecule acceptors(PSMAs) have very recently received wide attention because they possess advantages such as synthetic flexibility, narrowed bandgap, low energy loss, and impressive mechanical properties. With efforts on design and synthesis of PSMAs and polymer donors, significant progress has been made on all polymer solar cells(allPSCs) with power conversion efficiencies exceeding 18%. In this review, we focus on structure-property-performance relationships of the A-DA'D-A type PSMAs. First, we in-depth review the regio-random, regio-regular, and random ternary series by focusing on their structural modification such as from aspects of side-chains, halogenation, selenophene-containing and linkers, respectively. Second, we review the mechanically flexible and stretchable properties, which helps to find structural gene that correlates the mechanical properties. Third, we review the impressive small energy loss. In all, this review provides structural and material's clues, helpfully for designing high-performance all-PSCs.

关键词： all-polymer solar cell polymerized small-molecule acceptor regio-random regio-regular terpolymer energy loss

Modeling wave attenuation by vegetation with accompanying currents in SWAN

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Acta Oceanologica Sinica 2023年第12期42卷 63-76页

作者： Hong Wang zhan Hu School of Marine Sciences Sun Yat-Sen University/Southern Marine Science and Engineering Guangdong Laboratory(Zhuhai)Zhuhai 519000China State Key Laboratory of Internet of Things for Smart City/Department of Ocean Science and Technology University of MacaoMacao 999078China Guangdong Provincial Key Laboratory of Marine Resources and Coastal Engineering Guangzhou 510000China Pearl River Estuary Marine Ecosystem Research Station Ministry of EducationZhuhai 519000China

Coastal wetlands such as salt marshes and mangroves provide important protection against stormy waves.Accurate assessments of wetlands’capacity in wave attenuation are required to safely utilize their protection services.Recent studies have shown that tidal currents have a significant impact on wetlands’wave attenuation capacity,but such impact has been rarely considered in numerical models,which may lead to overestimation of wave attenuation in wetlands.This study modified the SWAN(Simulating Waves Nearshore)model to account for the effect of accompanying currents on vegetation-induced wave dissipation.Furthermore,this model was extended to include automatically derived vegetation drag coefficients,spatially varying vegetation height,and Doppler Effect in combined current-wave flows.Model evaluation against an analytical model and flume data shows that the modified model can accurately simulate wave height change in combined current-wave flows.Subsequently,we applied the new model to a mangrove wetland on Hailing Island in China with a special focus on the effect of currents on wave dissipation.It is found that the currents can either increase or decrease wave attenuation depending on the ratio of current velocity to the amplitude of the horizontal wave orbital velocity,which is in good agreement with field observations.Lastly,we used Hailing Island site as an example to simulate wave attenuation by vegetation under hypothetical storm surge conditions.Model results indicate that when currents are 0.08–0.15 m/s and the incident wave height is 0.75–0.90 m,wetlands’wave attenuation capacity can be reduced by nearly 10%compared with pure wave conditions,which provides implications for critical design conditions for coastal safety.The obtained results and the developed model are valuable for the design and implementation of wetland-based coastal defense.The code of the developed model has been made open source,in the hope to assist further research and coastal management.

关键词： wave attenuation by vegetation wave-current interaction SWAN model storm waves drag coefficient