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Transcriptomic and genetic approaches reveal an essential role of the NAC transcription factor SlNAP1 in the growth and defense response of tomato

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Horticulture Research 2020年第1期7卷 29-39页

作者： Jiao Wang Chenfei Zheng Xiangqi Shao Zhangjian Hu Jianxin Li Ping Wang Anran Wang Jingquan Yu Kai Shi Department of Horticulture Zhejiang University866 Yuhangtang Road310058 HangzhouPeople’s Republic of China

With global climate change,plants are frequently being exposed to various stresses,such as pathogen attack,drought,and extreme temperatures.Transcription factors(TFs)play crucial roles in numerous plant biological processes;however,the functions of many tomato(Solanum lycopersicum L.)TFs that regulate plant responses to multiple stresses are largely unknown.Here,using an RNA-seq approach,we identified SlNAP1,a NAC TF-encoding gene,which was strongly induced by various stresses.By generating SlNAP1 transgenic lines and evaluating their responses to biotic and abiotic stresses in tomato,we found that SlNAP1-overexpressing plants showed significantly enhanced defense against two widespread bacterial diseases,leaf speck disease,caused by Pseudomonas syringae pv.tomato(Pst)DC3000,and root-borne bacterial wilt disease,caused by Ralstonia solanacearum.In addition,SlNAP1 overexpression dramatically improved drought tolerance in tomato.Although the SlNAP1-overexpressing plants were shorter than the wild-type plants during the early vegetative stage,eventually,their fruit yield increased by 10.7%.Analysis of different hormone contents revealed a reduced level of physiologically active gibberellins(GAs)and an increased level of salicylic acid(SA)and abscisic acid(ABA)in the SlNAP1-overexpressing plants.Moreover,EMSAs and ChIP-qPCR assays showed that SlNAP1 directly activated the transcription of multiple genes involved in GA deactivation and both SA and ABA biosynthesis.Our findings reveal that SlNAP1 is a positive regulator of the tomato defense response against multiple stresses and thus may be a potential breeding target for improving crop yield and stress resistance.

关键词： breeding drought eventually

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一阶速度—胀缩—旋转弹性波方程交错网格数值模拟

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石油地球物理勘探 2022年第6期57卷 1352-1361,I0004页

作者：王辉何兵寿邵祥奇中国海洋大学海底科学与探测技术教育部重点实验室山东青岛266100 青岛海洋科学与技术国家实验室海洋矿产资源评价与探测技术功能实验室山东青岛266100 山东省烟台市自然资源和规划局山东烟台264003

弹性波正演在地震波传播机理研究以及多波地震资料采集、处理、解释和反演中发挥着重要作用。现有的弹性波方程正演模拟常常数值求解一阶速度—应力方程或二阶位移弹性波方程,只能直接得到同时包含纵波和横波的三个质点振动速度分量或... 详细信息

弹性波正演在地震波传播机理研究以及多波地震资料采集、处理、解释和反演中发挥着重要作用。现有的弹性波方程正演模拟常常数值求解一阶速度—应力方程或二阶位移弹性波方程,只能直接得到同时包含纵波和横波的三个质点振动速度分量或位移分量,要想得到更直观的纯纵波和纯横波分量记录,还需要在模拟过程中采用波场解耦算子进行纵、横波分离,因此纵、横波模拟精度同时受制于模拟算法和波场解耦算法的精度。为此,推导了一阶速度—胀缩—旋转弹性波方程在三维交错网格空间中的高阶有限差分格式,并给出了相应的稳定性条件;推导了适应该方程的PML吸收边界条件,实现了一阶速度—胀缩—旋转弹性波方程的正演模拟;分析了模拟结果中各分量的物理意义。由于一阶速度—胀缩—旋转弹性波方程不仅包含了质点的振动速度矢量,而且显式地包含了横波振动速度矢量和纵波振动速度矢量,还包含了一个体应变和一个旋转矢量,因此应用该方程模拟除了能得到三个质点振动速度分量外,还可以直接得到解耦后的纵、横波分量,避免了解耦算法对模拟精度的影响。模型试算证明了该模拟方法的正确性和优越性。

关键词：一阶速度—胀缩—旋转弹性波方程交错网格正演模拟有限差分 PML吸收边界条件纵、横波分离

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基于分频编码的弹性波全波形反演

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石油地球物理勘探 2020年第6期55卷 1321-1329,1165页

作者：邵祥奇何兵寿史才旺中国海洋大学海底科学与探测技术教育部重点实验室山东青岛266100 青岛海洋科学与技术国家实验室海洋矿产资源评价与探测技术功能实验室山东青岛266100 南方科技大学地球与空间科学系广东深圳518000

计算量与计算效率限制了全波形反演方法的应用,震源编码技术可以有效减少全波形反演的计算量,提升反演效率。传统震源编码技术要求各炮具有相同的接收点排列,在观测系统适应性方面存在不足,无法直接应用于滚动排列观测系统。为此,提出... 详细信息

计算量与计算效率限制了全波形反演方法的应用,震源编码技术可以有效减少全波形反演的计算量,提升反演效率。传统震源编码技术要求各炮具有相同的接收点排列,在观测系统适应性方面存在不足,无法直接应用于滚动排列观测系统。为此,提出一种基于分频编码的弹性波全波形反演方法,即在正演过程中仍可同时对多个炮记录进行正向延拓,给每一炮赋以不同频率的谐波震源,使同时进行正向延拓的各炮波场在频谱上互不重合;在炮点波场正传和残差反传过程中,采用相位灵敏度检测技术提取每炮的单频波场,实现各炮波场的完全分离,用于构建波形反演梯度,实现模型更新。同传统震源编码技术相比,该方法不受观测系统限制,能应用于排列滚动的观测系统,具有更广的应用范围。

关键词：弹性波全波形反演震源编码分频编码相位灵敏度检测

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Collective and Individual Assessment of the Risk of Death from COVID-19 for the Elderly,2020–2022

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China CDC weekly 2023年第18期5卷 407-412,I0001,I0002页

作者： Chaobao Zhang Hongzhi Wang Zilu Wen Zhijun Bao xiangqi Li Shanghai Key Laboratory of Clinical Geriatric Medicine Department of Geriatric MedicineHuadong HospitalShanghai Medical CollegeFudan UniversityShanghaiChina Shanghai Key Laboratory of Magnetic Resonance Research Center for Artificial Intelligence in Medical ImagingEast China Normal UniversityShanghaiChina Department of Scientific Research Shanghai Public Health Clinical CenterShanghaiChina Department of Endocrinology and Metabolism Gongli HospitalNaval Medical UniversityShanghaiChina

Introduction:Coronavirus disease 2019(COVID-19)has had profound disruptions worldwide.For a population or individual,it is critical to assess the risk of death for making preventative decisions.Methods:In this study,clinical data from approximately 100 million cases were statistically analyzed.A software and an online assessment tool were developed in Python to evaluate the risk of mortality.Results:Our analysis revealed that 76.51%of COVID-19-related fatalities occurred among individuals aged over 65 years,with frailty-associated deaths accounting for more than 80%of these cases.Furthermore,over 80%of the reported deaths involved unvaccinated individuals.A notable overlap was observed between aging and frailty-associated deaths,both of which were connected to underlying health conditions.For those with at least two comorbidities,the proportion of frailty and the proportion of COVID-19-related death were both close to 75 percent.Subsequently,we established a formula to calculate the number of deaths,which was validated using data from twenty countries and regions.Using this formula,we developed and verified an intelligent software designed to predict the death risk for a given population.To facilitate rapid risk screening on an individual level,we also introduced a six-question online assessment tool.Conclusions:This study examined the impact of underlying diseases,frailty,age,and vaccination history on COVID-19-related mortality,resulting in a sophisticated software and a user-friendly online scale to assess mortality risk.These tools offer valuable assistance in informed decision-making.

关键词： Python deaths software

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Morphological and Phylogenetic Study Based on New Materials ofAnchiornis huxleyi (Dinosauria, Theropoda) from Jianchang, Western Liaoning, China

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Acta Geologica Sinica(English Edition) 2018年第1期92卷 1-15页

作者： GUO xiangqi XU Li JIA Songhai Yunnan Provincial Museum Kunming 650000 Yunnan China Institute of Geology Chinese Academy of Geological Sciences Beijing 100037 China Henan Geological Museum Zhengzhou 450000 Henan China

Anchiornis huxleyi, which is a member of the Middle-Late Jurassic Yanliao Biota, is the smallest feathered dinosaur ever known. It has been described as a critical link between feathered dinosaurs and birds. Recent studies, including those of Anchiornis, Xiaotingia, Eosinopteryx and Aurornis, challenged Archaeopteryx as the most basal bird. The new Anchiornis huxleyi specimens that are described in this paper show some minor different characters compared to previously reported Anchiornis specimens, which has revised the character list of Anchiornis and indicates a different phylogenetic point from former opinions.

关键词： Anchiornis feathered dinosaur non-avian dinosaur Middle-Late Jurassic Yanliao Biota

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Survey of Framer Credit Demands in China with Tianjin as an Example

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Asian Agricultural Research 2024年第3期16卷 25-28,32页

作者： xiangqi LIU Zijian WU Rural Revitalization Strategy Research Institute Tianjin Normal UniversityTianjin 300387China School of Economics Tianjin Normal UniversityTianjin 300387China

Giving farmers more rights and interests on land property is the basis of achieving balanced and coordinated economic development between urban and rural areas as well as building a sharing economy during the period of economic transition in China.Based on the survey data of suburban households of Tianjin,in this paper,measurement method was adopted to study factors influencing farmer s willingness on land management right mortgage.The results shows that the willingness of individual farmer on mortgage is not strong,however,their willingness on mortgage is bound to have a significant relationship with farmland right confirmation and land transfer and other factors.Finally,it is concluded that it is necessary to promote land transfer on the basis of land right of confirmation while carrying out farmland mortgage under the premise of large-scale operation in China.

关键词： Willingness of mortgage Influencing factors Right of confirmation Land transfer

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基于工程教育认证的材料科学基础课程建设

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高等建筑教育 2017年第6期26卷 80-82页

作者：彭开萍邵艳群李湘祁福州大学材料科学与工程学院福建福州350116

以工程教育认证为导向,以提高学生的学习积极性为目的,在分析工程教育认证对材料科学与工程专业要求的基础上,从材料科学基础课程师资、教学大纲、教学方法、考试方式,以及教学质量改进等方面对课程综合改革进行探索,使其达到工程教育... 详细信息

以工程教育认证为导向,以提高学生的学习积极性为目的,在分析工程教育认证对材料科学与工程专业要求的基础上,从材料科学基础课程师资、教学大纲、教学方法、考试方式,以及教学质量改进等方面对课程综合改革进行探索,使其达到工程教育认证对专业的培养目标和毕业要求。

关键词：材料科学基础工程教育认证教学方式教学目标

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Dexamethasone and compliance affect TTFields efficacy to glioblastoma patients:a systematic review and meta-analysis

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Chinese Neurosurgical Journal 2022年第4期8卷 292-299页

作者： Shupeng Li Jiawei Dong Xinyu Wang xiangqi Meng Chuanlu Jiang Jinquan Cai Department of Neurosurgery The Second Affiliated Hospital of Harbin Medical UniversityHarbin 150086China

TTFields is a novel treating modality of glioblastoma(GBM)which can significantly prolong the overall survival(OS)of newly diagnosed or recurrent glioblastoma.Some researchers have revealed that a variety of factors can affect the efficacy of TTFields.So,we review the available literature about the influencing factors on efficacy of TTFields and then choose two experimentally supported factors:the dose of dexamethasone and compliance of TTFields to perform a meta-analysis.The PubMed,Embase,and the Cochrane Library are searched.Five articles are identified between 2014 and 2017.Three articles are about the compliance of TTFields.Two articles are about the dose of dexamethasone.The Newcastle-Ottawa Quality Assessment Scale(NOS)is used as an assessment tool to evaluate the methodological quality of all included trials.The scale’s range varies from 0 to 9 stars.According to the Cochrane Handbook for Systematic Reviews of Interventions,articles are graded in six items to evaluate the risk of bias.Two reviewers rate the studies independently and the final decision is reached by consensus.Our data shows that the median OS is conspicuously longer in the TTFields group in which the dose of dexamethasone is≤4.1 mg,WMD=9.23[95%CI 5.69-12.78];P<0.05).And the patients whose compliance of TTFields treatment≥75%(≥18 h per day)have a significant lower overall survival risk than the patients whose compliance of TTFields treatment<75%(HR=0.57[95%CI 0.46-0.70];P<0.00001).TTFields is a safe and efficient novel treatment modality.The dose of dexamethasone≤4.1 mg of TTFields treatment and the compliance of TTFields treatment≥75%,≥18 h per day are beneficial to the prognosis of the glioblastoma patients.

关键词： TTFields Glioblastoma Dexamethasone Compliance Meta-analysis

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Occurrence Factors and Comprehensive Prevention and Control of Chinese Rose Black Spot(Actinonema rosae)

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Plant Diseases and Pests 2020年第4期11卷 6-7,12页

作者： Hu xiangqi Hongpu Town Agricultural Station of Huaining County Anqing 246141China

In addition to conventional techniques,the prevention and control of Chinese rose black spot(Actinonema rosae)should be strengthened in the management of Chinese rose.The occurrence of Chinese rose black spot is closely related to plant residues,rainfall,cultivation conditions and varieties.On the basis of understanding the symptoms and occurrence regularity of Chinese rose black spot,combination measures of agricultural and chemical prevention and control should be taken.

关键词： Chinese rose black spot(Actinonema rosae) Occurrence factors Comprehensive prevention and control

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以学生为主体的任务驱动教学法在研究生教学中的探索与应用

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科技创新导报 2015年第27期12卷 129-130页

作者：邵艳群彭开萍李湘祁福州大学材料科学与工程学院福建福州350108

新形势下必须探索新的教学方法以推动研究生教学改革,任务驱动教学法是学习者在教师引导下自主构建知识体系的一种积极有效的教学方法。围绕任务驱动法的基本要素,在《交流阻抗法》教学中进行实践,通过制定具有可操作性的明确的教学目标... 详细信息

新形势下必须探索新的教学方法以推动研究生教学改革,任务驱动教学法是学习者在教师引导下自主构建知识体系的一种积极有效的教学方法。围绕任务驱动法的基本要素,在《交流阻抗法》教学中进行实践,通过制定具有可操作性的明确的教学目标,凝练任务使设计的任务在实施过程中达到教学上和逻辑上的连贯与流畅,解析任务,体现趣味性,输入具有实用性原则材料,执行任务的过程中学生始终保持主体的角色,展现真实情景等,为研究生搭建了一个科学高效的学习平台,激发学生学习的主体精神,为创新创造提供了良好的学习氛围。

关键词：研究生课程教学模式任务驱动教学法

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