检索结果-南通市图书馆

维普期刊数据库

Precore mutation enhances viral replication to facilitate persistent infection especially in HBeAg-negative patients

在线全文

学校读者我要写书评

暂无评论

Virologica Sinica 2024年第2期39卷 319-330页

作者： Guixin Li Danli Yang Xin Liu Ting Zhang Hui Liu Jun Zou Zimeng Xu xiangmei chen Lizhong Dai Hongsong chen Fengmin Lu Peking University People's Hospital Peking University Hepatology InstituteBeijing Key Laboratory of Hepatitis C and Immunotherapy for Liver DiseasesBeijing International Cooperation Base for Science and Technology on NAFLD DiagnosisBeijing100044China Department of Microbiology and Infectious Disease Center School of Basic Medical SciencesPeking UniversityBeijing100191China Baruch S.Blumberg Institute DoylestownPA18901USA Shenzhen Sanyuansheng Biotechnology Co. LtdShenzhen518000China Precision Medicine Center of Zhengzhou University Academy of Medical SciencesZhengzhou UniversityZhengzhou450001China Peking University-Sansure Biotech Joint Laboratory of Molecular Medicine Sansure Biotech Co.LtdChangsha410205China

Naturally occurred precore(PC,G1896A)and/or basal core promoter(BCP,A1762T/G1764A)mutations are prevalent in chronic HBV-infected patients,especially those under HBeAg-negative status.However,the replicative capacity of HBV with PC/BCP mutations remains ambiguous.Herein,meta-analysis showed that,only under HBeAg-negative status,the serum HBV DNA load in patients with PC mutation was 7.41-fold higher than those without the mutation.Both PC mutation alone and BCPþPC mutations promoted HBV replication in cell and hydrodynamic injection mouse models.In human hepatocyte chimeric mouse model,BCPþPC mutations led to elevated replicative capacity and intrahepatic core protein accumulation.Mechanistically,preC RNA harboring PC mutation could serve as mRNA to express core and P proteins,and such pgRNA-like function favored the maintenance of cccDNA pool under HBeAg-negative status.Additionally,BCPþPC mutations induced more extensive and severe human hepatocyte damage as well as activated endoplasmic reticulum stress and TNF signaling pathway in livers of chimeric mice.This study indicates that HBeAg-negative patients should be monitored on HBV mutations regularly and are expected to receive early antiviral treatment to prevent disease progression.

关键词： Hepatitis B virus Precore mutation Basal core promoter mutations Endoplasmic reticulum stress

Distribution of Traditional Chinese Medicine syndromes in diabetic kidney disease chronic kidney disease 1-5: a correlation study

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Traditional Chinese Medicine 2024年第3期44卷 572-580页

作者： LI Shuang DUAN Shuwei DONG Zheyi QU Yilun LUO Yayong KE Jianghua WANG Conghui PENG Yangzhi ZHOU Xuefeng chen xiangmei Department of Nephrology First Medical Center of Chinese PLA General HospitalNephrology Institute of the Chinese People's Liberation ArmyNational Key Laboratory of Kidney DiseasesNational Clinical Research Center for Kidney DiseasesBeijing Key Laboratory of Kidney Disease Research School of Clinical Medicine Guangdong Pharmaceutical University Basic Medical College Chengdu University of Traditional Chinese Medicine

OBJECTIVE: To analyze the distribution of Traditional Chinese medicine(TCM) syndromes in patients with diabetic kidney disease(DKD) and its related factors.METHODS: We enrolled 435 patients with DKD, who were not undergoing dialysis, admitted to the Department of Nephrology, First Medical Center, Chinese PLA General Hospital from April 2020 to August 2021.Analysis of their TCM syndromes and related factors was carried out.RESULTS: The 435 patients included 109, 117, 86, and 123 chronic kidney disease(CKD) 1-2, CKD3, CKD4, and CKD5 cases, respectively. With the progression of CKD1-5, the proportion of Yin deficiency and dry heat syndrome,and that of Qi and Yin deficiency syndrome showed a downward trend, whereas the proportion of spleen-kidney Yang deficiency, blood deficiency, blood stasis, water stagnation, and phlegm turbidity syndromes showed an upward trend; the differences were statistically significant(P < 0.05). Multivariate logistic regression analysis showed that Yin deficiency and dry heat syndrome was positively correlated with hemoglobin [odds ratio(OR) =1.022, P = 0.005], albumin(OR = 1.058, P = 0.006), and estimated glomerular filtration rate(eGFR)(OR = 1.020,P < 0.001) but negatively correlated with male sex(OR =0.277, P = 0.004). Qi and Yin deficiency syndrome was positively correlated with albumin(OR = 1.056, P < 0.001)and eGFR(OR = 1.008, P = 0.022) but negatively correlated with age(OR = 0.977, P = 0.023). Liver-kidney Yin deficiency syndrome was positively correlated with age(OR = 1.028, P = 0.021) and glycosylated hemoglobin(OR = 1.223, P = 0.007) but negatively correlated with total cholesterol(OR = 0.792, P = 0.006).Spleen-kidney Yang deficiency syndrome was negatively correlated with hemoglobin(OR = 0.977, P < 0.001),albumin(OR = 0.891, P < 0.001), and eGFR(OR = 0.978,P < 0.001) but positively correlated with high density lipoprotein(OR = 3.376, P = 0.001). CONCLUSION: With CKD1-5 progression, TCM syndromes changed from Yin deficiency and dry heat syndrome to syndrome of deficiency of both

关键词： albumins correlation of data diabetic nephropathies glomerular filtration rate hemoglobins syndrome and complex

Sugemule-10:Source of prescription and modern clinical application

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Chinese Herbal Medicines 2022年第3期14卷 376-384页

作者： xiangmei chen Liangliang Hadanbaoligao Zhang Buriebao Xiulan Wang Wurihan Tai Affiliated Hospital of Inner Mongolia Minzu University Tongliao 028005China NMPA Key Laboratory for Quality Control of Traditional Chinese Medicine(Mongolian Medicine) Tongliao 028000China National and Local Joint Engineering Research Center for Mongolian Medicine Research and Development(Development and Reform Commission) Tongliao 028000China Key Laboratory of Mongolian Medicine R&D Engineering Ministry of EducationTongliao 028000China Mongolian Medicine Research and Health Science Center of Inner Mongolia Minzu University Tongliao 028000China

Sugemule-10,one of the traditional Mongolian medicine(TMM)formulae,is derived from Four Medical Classics(Vol.4)and composed of 10 Mongolian medicines.It is used to treat kidney cold,low back pain,urinary obstruction,kidney/bladder stones,and is the main prescription for kidney cold.The current research on Sugemule-10 is mostly focused on its clinical efficacy,and few papers are available upon its historical changes.Therefore,we systematically reviewed Sugemule-10 from the aspects of prescription source,prescription interpretation,efficacy evolution,and modern clinical applications.

关键词： clinical application prescription interpretation prescription source Sugemule-10

维普期刊数据库

Composition analysis of Compound Shenhua Tablet,a seven-herb Chinese medicine for IgA nephropathy:evaluation of analyte-capacity of the assays

在线全文

学校读者我要写书评

暂无评论

Chinese Journal of Natural Medicines 2024年第2期22卷 178-192页

作者： ZHANG Haiyan WANG Qiuyue WANG Jianan ZHANG Sichao JIA Weiwei HE Ning XIA Xiaoyan WANG Ting LAI Liyu LI Jiaying DU Jing OLALEYE Olajide E chen xiangmei YANG Junling LI Chuan Graduate School Tianjin University of Traditional Chinese MedicineTianjin 301617China State Key Laboratory of Drug Research Shanghai Institute of Materia MedicaChinese Academy of SciencesShanghai 201203China School of Pharmacy University of Chinese Academy of SciencesChinese Academy of SciencesBeijing 100049China Department of Nephrology First Medical Center of Chinese PLA General HospitalNephrology Institute of the Chinese People’s Liberation ArmyNational Key Laboratory of Kidney DiseasesNational Clinical Research Center for Kidney DiseasesBeijing Key Laboratory of Kidney Disease ResearchBeijing 100853China Zhongshan Institute for Drug Discovery Zhongshan 528400China Haihe Laboratory of Modern Chinese Medicine Tianjin 301617China

Compound Shenhua Tablet,a medicine comprising seven herbs,is employed in treating IgA nephropathy.This study aimed to meticulously analyze its chemical composition.Based on a list of candidate compounds,identified through extensive literature review pertinent to the tablet’s herbal components,the composition analysis entailed the systematic identification,characterization,and quantification of the constituents.The analyte-capacity of LC/ESI-MS-based and GC/EI-MS-based assays was evaluated.The identified and characterized constituents were quantified to determine their content levels and were ranked based on the constituents’daily doses.A total of 283 constituents,classified into 12 distinct categories,were identified and characterized in the Compound Shenhua Tablet.These constituents exhibited content levels of 1−10982μg·g^(−1),with daily doses of 0.01−395μmol·d^(−1).The predominant constituents,with daily doses of≥10μmol·d^(−1),include nine organic acids(citric acid,quinic acid,chlorogenic acid,cryptochlorogenic acid,gallic acid,neochlorogenic acid,isochlorogenic acid C,isochlorogenic acid B,and linoleic acid),five iridoids(specnuezhenide,nuezhenoside G13,nuezhenidic acid,secoxyloganin,and secologanoside),two monoterpene glycosides(paeoniflorin and albiflorin),a sesquiterpenoid(curzerenone),a triterpenoid(oleanolic acid),and a phenylethanoid(salidroside).Additionally,there were 83,126,and 55 constituents detected in the medicine with daily doses of 1–10,0.1–1,and 0.01–0.1μmol·d^(−1),respectively.The combination of the LC/ESI-MS-based and GC/EI-MS-based assays demonstrated a complementary relationship in their analyte-capacity for detecting the constituents present in the medicine.This comprehensive composition analysis establishes a solid foundation for further pharmacological research on Compound Shenhua Tablet and facilitates the quality evaluation of this complex herbal medicine.

关键词： Compound Shenhua Tablet Composition analysis Analyte-capacity

Cloning and Function Identification of a Phytoene Desaturase Gene from Eucommia ulmoides

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Phyton-International Journal of Experimental Botany 2023年第5期92卷 1377-1389页

作者： Jiali Wang xiangmei chen Xiaozhen Huang Yichen Zhao Degang Zhao The Key Laboratory of Plant Resources Conservation and Germplasm Innovation in Mountainous Region(Ministry of Education) College of Tea SciencesCollege of Life SciencesGuizhou UniversityGuiyang550000China Plant Conservation Technology Center Guizhou Key Laboratory of Agricultural BiotechnologyGuizhou Academy of Agricultural SciencesGuiyang550000China

The phytoene desaturase(PDS)encodes a crucial enzyme in the carotenoid biosynthesis pathway.Silencing or inhibiting PDS expression leads to the appearance of mottled,chlorosis,or albino leaves.In this study,the CDS sequence of EuPDS(Eucommia ulmoides Phytoene Desaturase)was first cloned and then PDS was silenced in Nicotiana benthamiana.Result showed the expression level of EuPDS in leaves was higher than that in the roots and stems.In N.benthamiana leaves,which were treated by Agrobacterium for 24 h,photo-bleaching was shown on the fresh leaves one week after injection and the transcript level of PDS was down-regulated during the period of emersion.This suggested that EuPDS could silence PDS of N.benthamiana,so as to cause the phenotype of leaf whitening.PDS is the main reporter gene involved in virus-induced gene silencing(VIGS).This study offered molecular evidence for identifying PDS gene involved in Carotenoid’s biosynthesis pathway and the regulation networks in E.ulmides.It also laid a useful foundation for study on leaf discoloration mechanism of other woody plants.

关键词： Eucommia ulmoides EuPDS virus-induced gene silencing TRV relative expression

Hypertension in patients with CKD in China： clinical characteristics and management

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Frontiers of Medicine 2017年第3期11卷 307-309页

作者： Guangyan Cai xiangmei chen Department of Nephrology Chinese PLA General Hospital Chinese PLA Institute of Nephrology State Key Laboratory of Kidney DiseasesNational Clinical Research Center for Kidney Diseases Beijing 100853 China

Chronic kidney disease （CKD） is a life-threatening disease that results in end-stage renal disease （ESRD） and consumes substantial health resources. In China, the prevalence of CKD in the general population is 10.... 详细信息

关键词： CHRONIC KIDNEY-DISEASE BLOOD-PRESSURE PREVALENCE AWARENESS RATES

Non-genetic mechanisms of diabetic nephropathy

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Frontiers of Medicine 2017年第3期11卷 319-332页

作者： Qiuxia Han Hanyu Zhu xiangmei chen Zhangsuo Liu Department of Nephrology Chinese PLA General Hospital Chinese PLA Institute of Nephrology State Key Laboratory of Kidney DiseasesNational Clinical Research Center for Kidney Diseases Beijing Key Laboratory of Kidney Diseases Beijing 100853 China Department of Nephrology The First Affiliated Hospital of Zhengzhou University Zhengzhou 450052 China

Diabetic nephropathy （DN） is one of the most common microvascular complications in diabetes mellitus patients and is characterized by thickened glomeruIar basement membrane, increased extracellular matrix formation, and podocyte loss. These phenomena lead to proteinuria and altered glomerular filtration rate, that is, the rate initially increases but progressively decreases. DN has become the leading cause of end-stage renal disease. Its prevalence shows a rapid growth trend and causes heavy social and economic burden in many countries. However, this disease is multifactorial, and its mechanism is poorly understood due to the complex pathogenesis of DN. In this review, we highlight the new molecular insights about the pathogenesis of DN from the aspects of immune inflammation response, epithelial-mesenchymal transition, apoptosis and mitochondrial damage, epigenetics, and podocyte-endothelial communication. This work offers groundwork for understanding the initiation and progression of DN, as well as provides ideas for developing new prevention and treatment measures.

关键词： diabetic nephropathy immune inflammatory response epithelial-mesenchymal transition apoptosis mitochondrial damage epigenetics podocyte-endothelial communication

Comparative risk of acute kidney injury among cancer patients treated with immune checkpoint inhibitors

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Cancer Communications 2023年第2期43卷 214-224页

作者： Fei Liu ZixianWang Xiaofan Li Zhen Zhang Yue Yang Junquan chen Dinghua chen Lingling Wu Xiangyu Liu Sujun Han Fangming Wang Wasilijiang Wahafu Yibo Gao Shancheng Ren Nianzeng Xing Guangyan Cai xiangmei chen Department of Urology National Cancer Center/National Clinical Research Center for Cancer/Cancer HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijingP.R.China Laboratory of Translational Medicine National Cancer Center/National Clinical Research Center for Cancer/Cancer HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijingP.R.China Department of Thoracic Surgery National Cancer Center/National Clinical Research Center for Cancer/Cancer HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijingP.R.China Department of Medical Oncology Sun Yat-sen University Cancer CenterGuangzhouGuangdongP.R.China Department of Nephrology First Medical Center of Chinese PLA General HospitalNephrology Institute of the Chinese People’s Liberation ArmyState Key Laboratory of Kidney DiseasesNational Clinical Research Center for Kidney DiseasesBeijing Key Laboratory of Kidney Disease ResearchBeijingP.R.China Department of Plastic Surgery Plastic Surgery HospitalChinese Academy of Medical SciencesPeking Union Medical CollegeBeijingPR China State Key Laboratory of Molecular Oncology National Cancer Center/National Clinical Research Center for Cancer/Cancer HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijingP.R.China Central Laboratory National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital&Shenzhen HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeShenzhenGuangdongP.R.China Department of Urology Shanghai Changzheng HospitalShanghaiP.R.China

With the development and introduction of immune checkpoint inhibitors(ICIs)in cancer patients,immune-related side effects have increasingly attracted attention.However,the risks of immune-related renal toxicity are poorly characterized.In this study,we performed a network meta-analysis(NMA)of ICI-related randomized clinical trials(RCTs)to elucidate the comparative risk of acute kidney injury(AKI)in cancer patients receiving different ICIs.We also sought to identify other factors potentially affecting the risk of AKI.PubMed and EMBASE were searched for peer-reviewed trial reports published between January 2000 and May 2021.Eligible studies were RCTs studying ICIs in cancer patients and reporting AKI data.We performed a frequentist NMA to evaluate the risk ratios for grade 1-5 and grade 3-5 AKI between the treatment groups.We also assessed the absolute incidence of AKI in the ICI-containing arm using traditional direct meta-analysis.Once significant heterogeneity was detected in a traditional direct meta-analysis,multivariable meta-regression analysis was applied to identify factors that significantly affected the absolute incidence of AKI.A total of 85 RCTs were included in this study.In the NMA for the risk of grade 1-5 and 3-5 AKI,ipilimumab showed a significantly higher risk than avelumab and durvalumab,whereas 1 mg/kg nivolumab plus 3 mg/kg ipilimumab(N1I3)showed a significantly higher risk than other groups.In terms of treatment ranking,durvalumab±low-dose tremelimumab and avelumab were consistently among the top three safest treatments for grade 1-5 or 3-5 AKI,whereas N1I3,ipilimumab and tremelimumab were consistently among the top three treatments with the highest risk for grade 1-5 or 3-5 AKI.Compared with other cancers,renal cell carcinoma and urothelial carcinoma showed a significantly higher risk of AKI.The incidence of AKI was significantly higher with ICI+chemotherapy than with ICI monotherapy.In this NMA involving largescale up-to-date ICI trials,we demonstrated the comparative safety of existing ICI

关键词： immune checkpoint inhibitors acute kidney injury cancer immune therapy side effects

“双减”政策影响家长再生育意愿吗?——基于全国29个省份的调查

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

华东师范大学学报（教育科学版） 2024年第6期42卷 75-85页

作者：陈祥梅宁本涛华东师范大学教育学部上海200062

家庭生育意愿关系到人口的长期均衡发展。当前我国面临较大的低生育风险,低生育现象已成为常态。大多数研究聚焦于生育政策对人口的影响,忽略了教育政策的重要影响。本研究基于29个省份10341名中小学家长的调查,使用倾向得分匹配方法实... 详细信息

家庭生育意愿关系到人口的长期均衡发展。当前我国面临较大的低生育风险,低生育现象已成为常态。大多数研究聚焦于生育政策对人口的影响,忽略了教育政策的重要影响。本研究基于29个省份10341名中小学家长的调查,使用倾向得分匹配方法实证检验“双减”政策认可度对家长再生育意愿的影响及其影响机制。结果发现,提高中小学家长“双减”政策认可度,可显著提升家长再生育意愿。认可“双减”政策的中小学家长的再生育意愿效应更高,平均高出14.6%,这一结果表明“双减”政策的正外部性已外溢到人口生育领域,“双减”政策对家庭再生育计划产生了积极的影响。家庭教育支出和教育焦虑在中小学家长对“双减”政策的认可度和家长再生育意愿之间起中介作用。因此,应进一步提高中小学家长对“双减”政策的认知水平,持续加大“双减”政策实施力度,强化政策配套治理,教育和人口生育领域应实施多层次综合策略,全面有效地提升国民再生育意愿。

关键词： “双减”政策再生育意愿影响效应倾向得分匹配中介机制

维普期刊数据库博看期刊