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Effect of elicitors, precursors and metabolic inhibitors on paclitaxel production by Taxus cuspidata cell culture

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Journal of Forestry Research 2016年第6期27卷 1257-1263页

作者： Shujie Wang Chun Li Hujun Wang xiangmei zhong Jing Zhao Yueying Tong Yajun Zhou College of Biological and Agricultural Engineering Jilin University Key Laboratory of Bionic Engineering (Ministry of Education) Jilin University College of Food Science and Engineering Jilin University

In this study, using Taxus cuspidata as a raw material, we obtained stable high-yielding cell lines by subculturing and quantified paclitaxel content using ultra- sonic extraction combined with TLC-UV spectrophotom- etry. In single factor and multiple factors tests to optimize design and study the effects of elicitors, precursors, and metabolic inhibitors on paclitaxel production by Taxus cuspidata cells, paclitaxel production reached 4.32 mg/L when 100 μmol/L methyl jasmonate, 20 mg/L salicylic acid, 400 mg/L phenylalanine and 2 mg/L gibberellin （GA3） were added to the culture medium of suspension cells. When adding metabolic adjustment factors on the 7th day of culture, extra- and intracellular paclitaxel production was the highest at 4.855 mg/L, paclitaxel release rate was 10.48 %, fresh mass and paclitaxel production of cell increased, respectively, by 6.08 and 11.57 %. By controlling the anabolism of paclitaxel, paclitaxel yield was significantly improved.

关键词： Elicitor Precursor Metabolic inhibitor Paclitaxel Cell culture Taxus cuspidata

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基于《难经》“伤寒有五”探析太阳温病

中华中医药杂志 2024年第3期39卷 1251-1254页

作者：李志浩侯佑柱钟燕春周计春柯向梅河北中医药大学基础医学院石家庄050091

分析《素问·热论》可知“今夫热病者,皆伤寒之类也”的“伤寒”非广义伤寒之义,而是指病因“伤于寒”。“伤寒有五”是对伤于寒所导致的5种热病的分类方法,其中“中风”“伤寒”为感而即病者,“湿温”“热病”“温病”皆为伏邪所致。... 详细信息

分析《素问·热论》可知“今夫热病者,皆伤寒之类也”的“伤寒”非广义伤寒之义,而是指病因“伤于寒”。“伤寒有五”是对伤于寒所导致的5种热病的分类方法,其中“中风”“伤寒”为感而即病者,“湿温”“热病”“温病”皆为伏邪所致。冬不藏精,伤寒后伏匿于少阴,随春升之阳气化热内动,外达太阳而发为太阳温病。治疗以清热养阴,托邪外出为基本大法,根据阴精亏损和里热程度的不同,可选用辛凉清透、苦寒泄热、养阴清热药,随证治之。

关键词：《伤寒论》伤寒有五广义伤寒伏气温病太阳温病

小儿豉翘清热颗粒联合奥司他韦治疗儿童甲型H1N1流感的效果及对免疫功能的影响

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中外医学研究 2024年第5期22卷 116-119页

作者：周艳玲钟香梅宿迁市第一人民医院江苏宿迁223800

目的:观察小儿豉翘清热颗粒联合奥司他韦治疗儿童甲型H1N1流感的效果及对免疫功能的影响。方法:选择2021年1月—2023年1月于宿迁市第一人民医院就诊的102例甲型H1N1流感患儿作为研究对象,采用随机数表法将其分为常规组(n=51)与中药组(n=... 详细信息

目的:观察小儿豉翘清热颗粒联合奥司他韦治疗儿童甲型H1N1流感的效果及对免疫功能的影响。方法:选择2021年1月—2023年1月于宿迁市第一人民医院就诊的102例甲型H1N1流感患儿作为研究对象,采用随机数表法将其分为常规组(n=51)与中药组(n=51)。两组均给予一般治疗与护理,常规组给予口服磷酸奥司他韦治疗,中药组在常规组基础上给予小儿豉翘清热颗粒口服治疗。比较两组临床疗效、中医症候积分、T淋巴细胞亚群水平与不良反应。结果:中药组治疗总有效率为96.08%,高于常规组的82.35%,差异有统计学意义(P0.05)。结论:小儿豉翘清热颗粒与奥司他韦联合使用可以有效治疗儿童甲型H1N1流感,缓解临床症状,提高免疫功能,且安全性较高。

关键词：儿童甲型H1N1流感小儿豉翘清热颗粒奥司他韦免疫功能

Recombineering enables genome mining of novel siderophores in a non-model Burkholderiales strain

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Engineering Microbiology 2023年第3期3卷 30-36页

作者： Xingyan Wang Haibo Zhou xiangmei Ren Hanna Chen Lin zhong Xianping Bai Xiaoying Bian Helmholtz International Lab for Anti-Infectives State Key Laboratory of Microbial TechnologyShandong University-Helmholtz Institute of BiotechnologyShandong UniversityQingdao 266237China

Iron is essential for bacterial survival,and most bacteria capture iron by producing siderophores.Burkholde-riales bacteria produce various types of bioactive secondary metabolites,such as ornibactin and malleobactin siderophores.In this study,the genome analysis of Burkholderiales genomes showed a putative novel siderophore gene cluster crb,which is highly similar to the ornibactin and malleobactin gene clusters but does not have pvdF,a gene encoding a formyltransferase for N-δ-hydroxy-ornithine formylation.Establishing the bacteriophage recom-binase Redγ-Redδβ7029 mediated genome editing system in a non-model Burkholderiales strain Paraburkholderia caribensis CICC 10960 allowed the rapid identification of the products of crb gene cluster,caribactins A-F(1-6).Caribactins contain a special amino acid residue N-δ-hydroxy-N-δ-acetylornithine(haOrn),which differs from the counterpart N-δ-hydroxy-N-δ-formylornithine(hOrn)in ornibactin and malleobactin,owing to the absence of pvdF.Gene inactivation showed that the acetylation of hOrn is catalyzed by CrbK,whose homologs proba-bly not be involved in the biosynthesis of ornibactin and malleobactin,showing possible evolutionary clues of these siderophore biosynthetic pathways from different genera.Caribactins promote biofilm production and en-hance swarming and swimming abilities,suggesting that they may play crucial roles in biofilm formation.This study also revealed that recombineering has the capability to mine novel secondary metabolites from non-model Burkholderiales species.

关键词： Burkholderiales Recombineering Siderophore Genome mining Caribactins

Adsorption characteristics of droplets applied on non-smooth leaf surface of typical crops

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International Journal of Agricultural and Biological Engineering 2016年第1期9卷 35-41页

作者： Wang Shujie Wang Hujun Li Chun zhong xiangmei Huang Hui Zhou Yajun College of Biological and Agricultural Engineering Jilin UniversityChangchun 130025China Key Laboratory of Bionic Engineering(Ministry of Education China)Jilin UniversityChangchun 130025China College of Food Science and Engineering Jilin UniversityChangchun 130025China

To further understand the adsorption characteristics of different-type leaf surfaces adsorbing pesticide droplets and reveal the adsorption mechanism of pesticide droplets on non-smooth leaves,non-smooth leaves of 12 kinds of typical target plants were investigated in this study.The parameters of surface morphological characteristics were measured,which include contact angle between leaves and water droplets,water holding capacity surface tension,polar component,dispersion component and other quantitative range of indicators and variation by modern means.The relationships between the indicators and water holding capacity were investigated respectively.The experimental results show that the number of trichomes,epidermal wax,morphology and distribution characteristics have influences on adsorption characteristics of the water droplets.There is a negative correlation between free energy of obverse side and the water holding capacity(R=−0.447)while the free energy of reverse side and the water holding capacity show a positive correlation(R=0.212).Also,there is a negative correlation between polar component of obverse side and the water holding capacity(R=−0.357)while the polar component of reverse side and the water holding capacity in plant leaves show a positive correlation(R=0.149).The research can provide a scientific theory for reasonable spraying of pesticide in the agricultural production,and can be a reference for the development of pesticide adjuvants and bionic pesticides.

关键词： surface of plant leaves adsorption characteristics contact angle water holding capacity surface tension

介孔二氧化硅基智能递送体系的构建及其在各类疾病治疗中的应用

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化学进展 2022年第3期34卷 696-716页

作者：钟琴周帅王翔美仲维丁晨迪傅佳骏南京理工大学化工学院南京210094 茂名市人民医院临床科研中心茂名525000

利用先进纳米技术开发的药物递送体系能够改善药物的理化性质和治疗效果,同时削弱其毒副作用,因而纳米药物递送体系成为现代药剂学研究的热点和主流方向。其中,介孔二氧化硅作为纳米载体的基质材料具有比表面积大、形貌结构可调、表面... 详细信息

利用先进纳米技术开发的药物递送体系能够改善药物的理化性质和治疗效果,同时削弱其毒副作用,因而纳米药物递送体系成为现代药剂学研究的热点和主流方向。其中,介孔二氧化硅作为纳米载体的基质材料具有比表面积大、形貌结构可调、表面易于修饰及生物相容性良好等优点,引发生物医学研究人员的广泛关注,为构筑新型智能药物递送体系提供了新的设计思路。本文就介孔二氧化硅基智能递送体系在设计构筑和疾病治疗应用等方面的最新研究进展进行了综述。首先,本文对介孔硅的发展历程、制备方法及结构特性进行了简要概述;其次,从药物装载和门控释放两大角度系统阐述了近些年介孔硅基智能递送体系的构建策略,重点介绍了各种刺激响应性介孔硅基递送体系的门控开关(如聚合物、无机纳米颗粒、超分子组装体及生物大分子等)及其可控释放机制;随后,详细描述了介孔硅基控释体系在各种类型疾病(包括癌症、细菌感染、糖尿病和阿尔茨海默病等)治疗中的应用进展;最后,总结和分析了介孔硅基智能纳米载体研究中存在的问题并对其未来发展作了展望。

关键词：介孔二氧化硅纳米颗粒刺激响应靶向递送药物装载可控释放疾病治疗

Analysis of Pathological data of renal biopsy at one single center in China from 1987 to 2012

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Chinese Medical Journal 2014年第9期127卷 1715-1720页

作者： Zhang Xueguang Liu Shuwen Tang Li Wu Jie Chen Pu Yin zhong Li Minxia Xie Yuansheng Cai Guangyan Wei Ribao Qiu Qiang Wang Yuanda Shi Suozhu Chen xiangmei Department of Nephrology State Key Laboratory of Kidney Diseases (2011DAV00088) National Clinical Medical Research Center for Kidney Disease (2013BAI09B05) Chinese People's Liberation Army General Hospital Beijing 100853 China

Background In China,the prevalence of chronic kidney disease has increased significantly.Many studies shows that the spectrum of kidney disease had changed in recent years.We retrospectively analyzed the pathological types of renal biopsy and its spectrum change at the General Hospital of the Chinese People＇s Liberation Army from December 1987 to December 2012,in order to offer new supporting evidences for further specifying the distribution of renal pathological types in China.Methods According to the ＂Revised Protocol for the Histological Typing of Glomerulopathy＂（WHO,1995）,pathological diagnosis of renal biopsy was classified,detection rate of each pathological type was summarized （i.e.,percentage of total renal biopsy cases）,study period was divided at an interval of 5 years,and age-stratified distribution change of main pathological types was analyzed.Results The proportion of pathological types in 11 618 cases of renal biopsy was as follows：primary glomerulonephritis （PGN,70.7％）,secondary glomerulonephritis （SGN,20.7％）,tubular-interstitial nephropathy （4.0％）,hereditary/rare nephropathy （0.3％）,end-stage renal disease （0.9％）,and unclassified renal disease （3.3％）.Among PGN,there was IgA nephropathy （IgAN,37.0％）,membranous nephropathy （MN,11.8％）,mesangial proliferative glomerulonephritis （MsPGN,8.9％）,minimal change disease （MCD,6.6％）,and focal segmental glomerulosclerosis （3.9％）.Among SGN there was lupus nephritis （LN,5.5％）,Henoch-Sch（o）nlein purpura glomerulonephritis （5.3％）,hepatitis B virus-associated nephritis （HBVAN,3.03％）,diabetic nephropathy （2.2％）,and hypertension/malignant hypertension-associated renal damage （1.9％）.Pathological data were analyzed from 1987-1992 to 2008-2012 （after age adjustment）.Detection rate of IgAN tended to rise （P ＜0.001）.Detection rates of MN and MCD rose significantly （P ＜0.001）,but detection rate of MsPGN dropped significantly （P ＜0.001）.Among SGN,detection rate of HBVAN tended to drop （P ＜0.001）.Conclusion In China,PGN was the most common glomerulopathy （mostly IgAN）,LN was

关键词： renal biopsy kidney disease detection rate

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论中国共产党的文化使命

重庆社会科学 2023年第11期 5-14页

作者：李海青钟香妹中共中央党校(国家行政学院)马克思主义学院北京100091 中共江西省委党校哲学教研部江西南昌330003

中国共产党是具有文化使命的马克思主义使命型政党。中国共产党的文化使命以马克思主义为指导,生成、发展于为人民谋幸福、为民族谋复兴、为人类谋进步、为世界谋大同的历史进程中。党的文化使命鲜明体现在党领导与执政的理念中,有效贯... 详细信息

中国共产党是具有文化使命的马克思主义使命型政党。中国共产党的文化使命以马克思主义为指导,生成、发展于为人民谋幸福、为民族谋复兴、为人类谋进步、为世界谋大同的历史进程中。党的文化使命鲜明体现在党领导与执政的理念中,有效贯穿于文化建设的全过程,具有引领性、人民性、接续性、世界性等特征。在新的起点上继续推动文化繁荣、建设文化强国、建设中华民族现代文明,已成为中国共产党新的文化使命。推进实现新的文化使命,建设中华民族现代文明,需要在习近平文化思想指引下,坚定文化自信的底气,秉持开放包容的胸怀,坚持守正创新的方法。

关键词：中国共产党文化使命马克思主义民族复兴中华民族现代文明

甘肃省鸳鸯池水库群生物治沙护库技术应用与发展

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水利水电快报 2022年第9期43卷 96-98,111页

作者：仲香梅金塔县鸳鸯池水库管理所甘肃酒泉735300

为了从根本上减少入库泥沙,解决水库淤积问题,根据西北多风沙的气候因素,鸳鸯池水库群引用了滴灌节水新技术,在水库群周边种草植树,增加植被,以生物固沙防护林带的形式进行治沙护库,取得了良好的效果。通过生物治沙,既保水土,又拦泥沙,... 详细信息

为了从根本上减少入库泥沙,解决水库淤积问题,根据西北多风沙的气候因素,鸳鸯池水库群引用了滴灌节水新技术,在水库群周边种草植树,增加植被,以生物固沙防护林带的形式进行治沙护库,取得了良好的效果。通过生物治沙,既保水土,又拦泥沙,大幅度减轻了尘对水库的填埋淤积危害,发挥了显著的固沙防淤护库作用。研究成果可为其他水库治沙护库工作提供参考。

关键词：生物治沙固沙防淤护库滴灌节水鸳鸯池水库群甘肃省