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Sulforaphane ameliorates non-alcoholic steatohepatitis by KLF4-mediated macrophage M2 polarization

Food Science and Human Wellness 2024年第5期13卷 2727-2740页

作者： xianghui huang Jia Xu Ye Xu Bingxin huangfu Feng Zhang Yanzhou Hu Ruxin Gao Xinxin Ren Boyang Zhang Kunlun huang Xiaoyun He Key Laboratory of Precision Nutrition and Food Quality Key Laboratory of Functional DairyMinistry of EducationCollege of Food Science and Nutritional EngineeringChina Agricultural UniversityBeijing 100083China Key Laboratory of Safety Assessment of Genetically Modified Organism(Food Safety) the Ministry of Agriculture and Rural Affairs of the P.R.ChinaBeijing 100083China

Non-alcoholic fatty liver disease (NAFLD) has become a global issue and a severe threat to public ***, to date, no approved therapeutic drugs have been developed. Dietary interventions with naturalproducts have shown promise in preventing and treating NAFLD. Sulforaphane (SFN) is a phytocompoundwith antioxidant and anti-inflammatory properties, and previous research has demonstrated that SFN canameliorate hepatic lipid accumulation and inflammation. However, the molecular mechanisms underlying thesebeneficial effects remain unclear. In this study, we confirmed the protective effects of SFN on excessive lipidaccumulation and inflammatory injury in a high-fat, high-fructose diet-induced non-alcoholic steatohepatitis(NASH) mouse model. We found that SFN attenuates the inflammatory injury in a macrophage cell line andthe liver of NASH mice, owing to the promotion of M1-type macrophage polarization toward the M2-type andthe regulation of inflammatory mediators. Further analysis demonstrated that this SFN-induced macrophageM2-type polarization occurs in a Krüppel-like factor 4 (KLF4)-dependent manner. In summary, we uncovereda new mechanism of action underlying SFN activity and provide evidence that dietary intervention with SFNmight be protective against NASH.

关键词： Non-alcoholic steatohepatitis(NASH) Krüppel-like factor 4 Nuclear translocation Chemokine Lipid metabolism

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Association of copy number variation in X chromosome-linked PNPLA4 with heterotaxy and congenital heart disease

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中华医学杂志(英文版) 2024年第15期137卷 2024 Jul 8页

作者： Gao Han huang xianghui Chen Weicheng Feng Zhiyu Zhao Zhengshan Li Ping Tan Chaozhong Wang Jinxin Zhuang Quannan Gao Yuan Min Shaojie Yao Qinyu Qian Maoxiang Ma Xiaojing Wu Feizhen Yan Weili Sheng Wei huang Guoying Children’s Hospital of Fudan University Shanghai China Shanghai Key Laboratory of Birth Defects Shanghai China Fujian Key Laboratory of Neonatal Diseases Xiamen Children’s Hospital Xiamen Fujian China Research Unit of Early Intervention of Genetically Related Childhood Cardiovascular Diseases Chinese Academy of Medical Sciences Shanghai China

Background: Heterotaxy (HTX) is a thoracoabdominal organ anomaly syndrome and commonly accompanied by congenital heart disease (CHD). The aim of this study was to analyze rare copy number variations (CNVs) in a HTX/CHD cohort and to examine the potential mechanisms contributing to HTX/***: Chromosome microarray analysis was used to identify rare CNVs in a cohort of 120 unrelated HTX/CHD patients, and available samples from parents were used to confirm the inheritance pattern. Potential candidate genes in CNVs region were prioritized via the DECIPHER database, andPNPLA4 was identified as the leading candidate gene. To validate, we generatedPNPLA4-overexpressing human induced pluripotent stem cell lines as well aspnpla4-overexpressing zebrafish model, followed by a series of transcriptomic, biochemical and cellular ***: Seventeen rare CNVs were identified in 15 of the 120 HTX/CHD patients (12.5%). Xp22.31 duplication was one of the inherited CNVs identified in this HTX/CHD cohort, andPNPLA4 in the Xp22.31 was a candidate gene associated with HTX/***4 is expressed in the lateral plate mesoderm, which is known to be critical for left/right embryonic patterning as well as cardiomyocyte differentiation, and in the neural crest cell lineage. Through a series ofin vivo andin vitro analyses at the molecular and cellular levels, we revealed that the biological function ofPNPLA4 is importantly involved in the primary cilia formation and function via its regulation of energy metabolism and mitochondria-mediated ATP ***: Our findings demonstrated a significant association between CNVs and HTX/CHD. Our data strongly suggested that an increased genetic dose ofPNPLA4 due to Xp22.31 duplication is a disease-causing risk factor for HTX/CHD.

关键词： PNPLA4 Copy number variations X-chromosome Heterotaxy Congenital heart diseases

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微尺度下场致发射对电晕放电的影响机理

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真空科学与技术学报 2024年第2期44卷 146-155页

作者：黄向慧刘振祖张晶园张蓓西安科技大学电气与控制工程学院西安710054 西安市电气设备状态检测与供电安全重点实验室西安710000 西安科技大学机械工程博士后流动站西安710054

场致离子化微电晕器件基于一维纳米材料的电场增强效应,可产生大气压非平衡微等离子体,适用于气体检测以及生物医疗等领域,具有响应快、低功耗等特点。但微尺度下的电晕放电属于局部自持的低能放电,其场致发射的作用及影响机制尚不清晰... 详细信息

场致离子化微电晕器件基于一维纳米材料的电场增强效应,可产生大气压非平衡微等离子体,适用于气体检测以及生物医疗等领域,具有响应快、低功耗等特点。但微尺度下的电晕放电属于局部自持的低能放电,其场致发射的作用及影响机制尚不清晰。因此文章基于流体-化学混合模型框架,融合F-N发射模型,建立了常温常压N2-O2混合气体的二维微电晕放电模型。通过与无场致发射放电模型对比,探索不同间距下场致发射对放电动态过程的影响,发现针-板间距减至15μm时,两种模型放电过程差异很小,表明放电以二次电子发射机制为主导,当间距减至10μm时,两种模型的放电强度相差近1倍,场致发射的作用及影响不可忽略;通过进一步对电子密度分布、空间电场分布、电流密度等参量进行动态分析,发现场致发射、空间碰撞电离反应和离子轰击二次电子发射之间存在链锁影响规律。

关键词：微电晕结构局部自持放电场致发射数值模拟

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Preparation and characteriza-tion of CoFe_2O_4/SiO_2 nanocomposites

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Chinese Science Bulletin 2006年第20期51卷 2529-2534页

作者： huang xianghui CHEN Zhenhua College of Materials Science and Engineering Hunan University Changsha 410082 China

CoFe_2O_4/SiO_2 nanocomposites 被一个大音阶的第五音胶化过程准备了，从 tetraethylorthosilicate 和金属性的硝酸盐开始。composites 的形成机制被微分扫描热量测定，红外线的光谱学，传播电子显微镜学， X 光检查衍射和拉曼光谱学... 详细信息

CoFe_2O_4/SiO_2 nanocomposites 被一个大音阶的第五音胶化过程准备了，从 tetraethylorthosilicate 和金属性的硝酸盐开始。composites 的形成机制被微分扫描热量测定，红外线的光谱学，传播电子显微镜学， X 光检查衍射和拉曼光谱学描绘。磁性用一个颤动的样品磁强计被测量。在低温度获得的 xerogel 是非结晶的，这被发现。在 400 deg C 的热处理导致了部分形成 ofCoFe_2O_4 簇。在进一步增加退火的温度到 800 deg C 以后，有约 17 nm 的直径的 CoFe_2O_4nanocrystals 能在硅石矩阵被驱散。CoFe_2O_4 簇的形成伴有在金属离子之间的相互作用的硅石网络和包围硅石网络的重新整理。当退火的温度被提起到时，在粒子和矩阵之间的相互作用将被削弱或甚至消失象 800 deg C 一样高。强制的地和 composites 的磁化的密度将随退火的温度的增加增加。

关键词： CoFe204/SiO2 纳米复合材料溶胶-凝胶磁性能

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喀喇昆仑-帕米尔地区山羊群体遗传多样性及系统发育分析

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中国畜牧兽医 2024年第6期51卷 2533-2544页

作者：黄翔辉张天能尔孜古·木沙王玉涛喀什大学生命与地理科学学院喀什844000 新疆帕米尔高原生物资源与生态重点实验室喀什844000 新疆哈密市伊吾县畜牧工作服务中心哈密839000

【目的】明确喀喇昆仑-帕米尔地区山羊群体遗传多样性及系统发育情况,为山羊种质资源保存、发掘和利用提供理论依据。【方法】以分布于喀喇昆仑-帕米尔地区的山羊为研究对象,对其线粒体(mtDNA)非编码序列置换环(D-loop)区和细胞色素b(Cy... 详细信息

【目的】明确喀喇昆仑-帕米尔地区山羊群体遗传多样性及系统发育情况,为山羊种质资源保存、发掘和利用提供理论依据。【方法】以分布于喀喇昆仑-帕米尔地区的山羊为研究对象,对其线粒体(mtDNA)非编码序列置换环(D-loop)区和细胞色素b(Cytb)基因序列进行PCR扩增并测序。利用Mega 7.0.26软件分析碱基组成,结合从GenBank中下载的山羊mtDNA D-loop区序列和Cytb基因序列绘制系统发育树,使用DNASP 5.10软件统计单倍型分布并进行中性检验,通过Network 10.2软件构建单倍型中介网络图。【结果】喀喇昆仑-帕米尔地区山羊mtDNA D-loop区序列和Cytb基因序列AT含量分别为60.1%和58.7%,AT含量均高于GC含量,具有碱基组成偏好性。D-loop区序列和Cytb基因分别存在163和84个SNPs位点,分别定义40、30种单倍型,单倍型多样性(Hd)分别为0.985和0.883,核苷酸多样性(Pi)分别为0.01570和0.00425。mtDNA D-loop区和Cytb基因的中性检验Tajima’s D值和Fu’s Fs值均<0,显著偏离中性,核苷酸错配分布图呈现3个峰。系统发育树分析表明喀喇昆仑-帕米尔地区山羊包含4个支系,分别为A、B、C、G。【结论】喀喇昆仑-帕米尔地区山羊至少经历过3次群体扩张事件,遗传多样性丰富,西亚地区的山羊对其存在基因渗入,同域的北山羊对其没有遗传贡献。

关键词：山羊喀喇昆仑-帕米尔地区 D-loop区 Cytb基因遗传多样性

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金刚石压机冷却水温控系统的设计

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机床与液压 2024年第4期52卷 93-99页

作者：黄祥辉朱晓东任天平苏宇锋郑州大学机械与动力工程学院河南郑州450001 郑州大学电气与信息工程学院河南郑州450001

针对当前金刚石压机冷却水温控系统使用传统PID算法控制精度低、温度波动大的问题,提出一种将改进鲸鱼算法与神经网络PID控制算法相结合的控制方法,实现PID参数的自适应调整。同时以GD32单片机为核心、步进电机为执行机构,设计循环冷却... 详细信息

针对当前金刚石压机冷却水温控系统使用传统PID算法控制精度低、温度波动大的问题,提出一种将改进鲸鱼算法与神经网络PID控制算法相结合的控制方法,实现PID参数的自适应调整。同时以GD32单片机为核心、步进电机为执行机构,设计循环冷却水温控系统硬件控制器来控制流量调节阀的开度,实现温度控制。仿真和实验结果表明:与传统PID算法相比,使用该温控系统进行温度控制时,超调量更小,控制精度更高,控制效果有很大提升。

关键词：金刚石压机冷却水温度改进鲸鱼算法 BP神经网络

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Establishment of a recombinant adeno-associated virus expressing hVEGF_(165)

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Neural Regeneration Research 2008年第6期3卷 610-613页

作者： xianghui huang Zhibin Shi Xiaoqian Dang Chen Zhang Pengbo Yu Kunzheng Wang Department of Orthopedics Second Affiliated Hospital of Xi＇an Jiaotong University Xi＇an 710004 Shaanxi Province China Laboratory of Shaanxi Provincial Center for Disease Control and Prevention Xi＇an 710054 Shaanxi Province China

BACKGROUND： Because certain gene vectors could have deleterious effects in the central nervous system, the choice of a safe and effective vector system has become more important for gene therapy of nerve regeneration. OBJECTIVE： To construct a non-pathogenic, recombinant adeno-associated virus （AAV） simultaneously expressing human vascular endothelial growth factor 165 （hVEGF165） and green fluorescent protein （GFP）. DESIGN, TIME AND SETTING： A randomized controlled experiment was performed at the Virology Laboratory of Shaanxi Provincial Center for Disease Control and Prevention between March and September 2007. MATERIALS： AAV helper-free system, AAV-293 packaging cell line, and AAV HT-1080 cells were purchased from Stratagene, USA. E. coli DH5α was a stocked strain from Centers for Disease Control and Prevention of Shaanxi, China. Plasmid pUC18-hHVEGF165 was a gift from Zhibin Shi. METHODS： The hVEGF165 gene was amplified by PCR from pUC18-hHVEGF165 and inserted into plasmid pAAV-IRES-hrGFP to construct recombinant plasmid pAAV-hVEGF165-IRES-hrGFP. Subsequently pAAV-hVEGF165-IRES-hrGFP, pAAV-RC （the rep/cap-gene containing plasmid）, and pHelper were co-transfected into AAV-293 cells to complete rAAV-hVEGF165-IRES-hrGFP packaging through homologous recombination. The efficiency of AAV packaging was monitored under a fluorescent microscope, and the recombinant viral particles were harvested from infected AAV-293 cells, and further concentrated and purified. AAV HT-1080 cells were infected with the recombinant virus AAV-hVEGF165-IRES-hrGFP. MAIN OUTCOME MEASURES： Recombinant virus titer was measured by fluorescent cell counting, and infection efficiency was detected by Fluorescence Activated Cell Sorter （FACS） upon infecting AAV-HT1080 cells. The recombination with the exogenous gene was verified by PCR. RESULTS： The PCR amplified products were verified as hVEGF165 gene by DNA sequencing, and the recombinant pAAV-hVEGF165-IRES-GFP was confirmed by double digestion. Th

关键词： vascular endothelial factor adeno-associated virus nerve regeneration

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Synthesis and reactivity of cobalt dinitrogen complex supported by nonsymmetrical pincer ligand

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Chinese Chemical Letters 2024年第7期35卷 259-263页

作者： Yuanjin Chen xianghui Shi Dajiang huang Junnian Wei Zhenfeng Xi Beijing National Laboratory for Molecular Sciences(BNLMS) Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of EducationCollege of ChemistryPeking UniversityBeijing 100871China State Key Laboratory of Organometallic Chemistry Shanghai Institute of Organic ChemistryShanghai 200032China

A nonsymmetrical PNN pincer ligand[6-(^(t)Bu_(2)PNH)C_(5)H_(4)N-2-(3-Mes)C_(3)H_(2)N_(2)]and its corresponding cobalt-N_(2)complex were synthesized and *** the stoichiometric reaction of the PNN ligand lithium salt with CoCl_(2),the complex 3,(PNN)CoCl,was ***,reduction of 3 with NaBHEt3under a dinitrogen atmosphere yielded complex 5,(PNN)Co(Ⅰ)(η^(1)-N_(2)).Single-crystal X-ray analysis,IR spectrum,and DFT calculations revealed that the dinitrogen in 5 was only weakly reduced by the cobalt *** reactions of 5 with carbon monoxide and 2,6-dimethylphenyl isocyanide gave carbonyl and isocyanide complexes 6 and 7 with the release of N_(2),***,these cobalt complexes,especially complex 5,demonstrated the capacity to convert dinitrogen to N(TMS)_(3)with moderate efficiency.

关键词： Dinitrogen fixation Dinitrogen transformation Nonsymmetrical pincer ligand Cobalt complex Catalytic silylamine formation

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Seasonal Variation of the Sea Surface Temperature Growth Rate of ENSO

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Advances in Atmospheric Sciences 2024年第3期41卷 465-477页

作者： Xinyi XING xianghui FANG Da PANG Chaopeng JI Department of Atmospheric and Oceanic Sciences and Institute of Atmospheric Sciences Fudan UniversityShanghai 200438China Shanghai Frontiers Science Center of Atmosphere-Ocean Interaction Shanghai 200438China Shanghai Key Laboratory of Ocean-land-atmosphere Boundary Dynamics and Climate Change Fudan UniversityShanghai 200438China

El Ni?o–Southern Oscillation(ENSO) exhibits a distinctive phase-locking characteristic, first expressed during its onset in boreal spring, developing during summer and autumn, reaching its peak towards winter, and decaying over the next spring. Several studies have demonstrated that this feature arises as a result of seasonal variation in the growth rate of ENSO as expressed by the sea surface temperature(SST). The bias towards simulating the phase locking of ENSO by many state-of-the-art climate models is also attributed to the unrealistic depiction of the growth rate. In this study, the seasonal variation of SST growth rate in the Ni?o-3.4 region(5°S–5°N, 120°–170°W) is estimated in detail based on the mixed layer heat budget equation and recharge oscillator model during 1981–2020. It is suggested that the consideration of a variable mixed layer depth is essential to its diagnostic process. The estimated growth rate has a remarkable seasonal cycle with minimum rates occurring in spring and maximum rates evident in autumn. More specifically, the growth rate derived from the meridional advection(surface heat flux) is positive(negative) throughout the year. Vertical diffusion generally makes a negative contribution to the evolution of growth rate and the magnitude of vertical entrainment represents the smallest contributor. Analysis indicates that the zonal advective feedback is regulated by the meridional immigration of the intertropical convergence zone, which approaches its southernmost extent in February and progresses to its northernmost location in September, and dominates the seasonal variation of the SST growth rate.

关键词： SST growth rate intertropical convergence zone zonal advective feedback mixed layer depth ENSO seasonal variation

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Scuffing failure analysis based on a multiphysics coupling model and experimental verification

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Friction 2024年第6期12卷 1214-1234页

作者： Bugao LYU xianghui MENG Jiabao YIN Yi CUI Chengen WANG School of Mechanical Engineering Shanghai Jiao Tong UniversityShanghai 200240China

General reductions in lubricant viscosities and increasing loads in machine components highlight the role of tribofilms in providing surface protection against ***,the relationship between the scuffing process and the growth and removal of tribofilm is not well *** this study,a multiphysics coupling model,which includes hydrodynamic lubrication,asperity contact,thermal effect,tribochemistry reaction,friction,and surface wear,was developed to capture the initiation of surface *** and experiments for a piston ring and cylinder liner contact were conducted following a step-load sequence under different temperature *** results show that high temperature and extreme load could induce the lubricant film collapse,which in turn triggers the breakdown of the tribofilm due to the significantly increased removal *** failures of both lubricant film and tribofilm progress instantaneously in a coupling way,which finally leads to severe scuffing.

关键词： scuffing failure tribofilm boundary lubrication multiphysics coupling effects

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