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基于转录因子的生物传感器研究进展

食品与发酵工业 2024年第12期50卷 362-369页

作者：赵梅罗佳璐王震周海星杨爽黎商洁齐向辉江苏大学食品与生物工程学院江苏镇江212013

转录因子的生物传感器(transcription-factor-based biosensors,TFBBs)作为一种重要的合成生物学工具,在高通量筛选、适应性进化、动态调控等方面具有重要作用。然而,天然的TFBBs由于灵敏度低、专一性差、响应慢等无法满足当前的应用需... 详细信息

转录因子的生物传感器(transcription-factor-based biosensors,TFBBs)作为一种重要的合成生物学工具,在高通量筛选、适应性进化、动态调控等方面具有重要作用。然而,天然的TFBBs由于灵敏度低、专一性差、响应慢等无法满足当前的应用需求。为应对上述挑战,该文简述了TFBBs的分类及其原理,重点介绍了TFBBs的性能调节策略,包括利用定向进化、蛋白质工程等策略优化生物传感器的灵敏度、特异性、检测范围和动态范围;总结了TFBBs在高通量筛选、适应性进化及动态调控方面的应用,讨论了TFBBs在实际应用中可能面临的机遇和挑战,并对其发展趋势进行了展望,指出了合成生物学的高速发展将促进生物传感器应用于更广泛的领域。

关键词：转录因子的生物传感器性能调节高通量筛选适应性进化动态调控

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基于非天然氨基酸的蛋白质合成研究进展

食品与发酵工业 2024年第12期50卷 336-342页

作者：赵梅刘馨远陈洋商洁丛宏日马豪 RAVIKUMAR Yuvaraj 齐向辉江苏大学食品与生物工程学院江苏镇江212013

蛋白质是生命体的重要组成部分,几乎参与了所有的生命活动。通常情况下生物体中蛋白质由20种天然氨基酸组成,但随着现代科技的飞速发展,人们对医疗、食品、材料等方面的需求日渐增大,这些蛋白质已经无法满足多样化的应用需求。随着非天... 详细信息

蛋白质是生命体的重要组成部分,几乎参与了所有的生命活动。通常情况下生物体中蛋白质由20种天然氨基酸组成,但随着现代科技的飞速发展,人们对医疗、食品、材料等方面的需求日渐增大,这些蛋白质已经无法满足多样化的应用需求。随着非天然氨基酸及其合成的蛋白质的优异性能,使得利用非天然氨基酸改造蛋白质的技术逐渐成为研究热点。非天然氨基酸有着不同于天然氨基酸的侧链基团,可以给蛋白质带来新的性质与功能。该文基于对非天然氨基酸及非天然蛋白质的研究,首先介绍了以化学及生物的手段合成非天然氨基酸的方法,同时对化学及生物法的优劣势及未来的发展前景进行了总结;然后介绍了将非天然氨基酸掺入蛋白质的几种热门方法;接着对非天然氨基酸及其合成蛋白质的应用在酶的改造、科研以及医疗方面进行了列举和描述;最后对这项技术未来的发展进行了展望与总结。

关键词：非天然氨基酸非天然蛋白质细胞代谢工程遗传密码扩展酶的改造蛋白药物改造

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金融资产管理公司高质量发展路径研究

西部金融 2024年第1期 20-27页

作者：李善燊郝琪梦齐祥会西安石油大学陕西西安710065 陕西金融资产管理股份有限公司陕西西安710021

金融资产管理公司作为处置金融风险的专营机构,走好自身高质量发展之路才能为金融强国贡献力量。当前,我国不良资产管理市场已形成多元化市场格局,不同类型金融资产管理公司因功能定位和业务重心的不同而呈现出一定差异性,然而仍存在主... 详细信息

金融资产管理公司作为处置金融风险的专营机构,走好自身高质量发展之路才能为金融强国贡献力量。当前,我国不良资产管理市场已形成多元化市场格局,不同类型金融资产管理公司因功能定位和业务重心的不同而呈现出一定差异性,然而仍存在主责主业不聚焦、创新不足与逾矩并存、市场定位不够准确清晰、绿色和开放功效有待提升等问题。本文从坚持主业主责提升综合金融服务能力、加快金融科技赋能与数字化改造、推动全国AMC与地方AMC的平衡有序发展、加强绿色化与国际化发展步伐四方面,提出金融资产管理公司高质量发展路径和相应政策建议。

关键词： AMC高质量发展差异化发展主责主业金融科技赋能

Systematic functional analysis and potential application of a serine protease from cold-adapted Planococcus bacterium

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Food Science and Human Wellness 2023年第5期12卷 1751-1761页

作者： Weijun Leng Xiaoyun Wu xianghui qi Hongying Liu Li Yuan Ruichang Gao School of Food and Biological Engineering Jiangsu UniversityZhenjiang 212013China Ocean College of Hebei Agriculture University Qinhuangdao 066000China

In this study, a gene encoding serine protease(PmSpr288)from cold-adapted bacterium, namely Planococcus maritimus XJ11, was cloned and overexpressed in Escherichia coli BL21(DE3). Bioinformatics analysis revealed that PmSpr288 belongs to serine protease S8 superfamily with a classical catalytic triad comprised by the Asp49, His86 and Ser251. Moreover, PmSpr288 was found to be active over broad alkaline pH and low-moderate temperature, and exhibited wide range of protein substrate specificity. In addition, PmSpr288 was able to hydrolyze the meat proteins actin and myosin, and molecular docking results suggested that the crucial interaction between PmSpr288 and actin/myosin complexes was mainly occupied by hydrogen bonds. The muscle protein hydrolysates of silver carp prepared by PmSpr288 was shown to have antioxidant activity via DPPH radical scavenging assay, which presented an IC_(50) valve of 1.309 mg/mL. In conclusion, these characteristics imply that PmSpr288 has potential biotechnological application prospect for the production of bioactive peptides.

关键词： Protease Silver carp Protein hydrolysates Molecular docking Bioactive peptides

Rational design of glycerol dehydratase:Swapping the genes encoding the subunitsof glycerol dehydratase to improve enzymatic properties

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Chinese Science Bulletin 2006年第24期51卷 2977-2985页

作者： qi xianghui SUN Liang LUO Zhaofei WU Jiequn MENG Xiaolei TANG Yue WEI Yutuo HUANG Ribo Guangxi Key Laboratory of Subtropical Bioresource Conservation and Utilization Guangxi University Nanning 530004 China Guangxi Academy of Sciences Nanning-530003 China Sinozyme Biotech. Co. Ltd. Nanning 530004 China

1,3-propanediol (1,3-PD) is an important material for chemical industry,and there has been always much interest in the production of 1,3-PD using all possible routes. The genes encoding glyc-erol dehydratase (GDHt) from Citrobacter freundii,Klebsiella pneumoniae and metagenome were cloned and expressed in E. coli. All glycerol dehy-dratases but the one from metagenome could be detected to show enzyme activities. In order to im-prove the enzymatic properties of GDHts,the genes encoding α and β-γ subunits were cloned,and the enzyme characteristics were evolved by rational de-sign based on their 3D structures which were con-structed by homology modeling. Six heteroenzymes were obtained by swapping the α subunit genes of these three different-source-derived GDHts. The pH,thermal stability and Vmax of some heteroenzymes were dramatically improved by 2―5 times compared with the wild one (GDHtKP). The GDHt cloned from metagenome,originally proved to be with no enzyme activity,was converted into active enzyme by swap-ping its subunits with other different GDHts. In addi-tion,the effect of subtle 3D structural changes on the properties of the enzyme was also observed.

关键词：甘油脱水酶基因交换技术异源同工酶设计异基因组基因克隆

Exploring the Predominant Factors Influencing the Oxygen Reduction Performance of PtCo/C Catalysts

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Chemical Research in Chinese Universities 2024年第4期40卷 753-760页

作者： LI Jinrong YU xianghui SUN qi PENG Yong CAO Shuang HOU Chun-Chao XU qiang College of Chemistry and Chemical Engineering Institute for Sustainable Energy and ResourcesQingdao UniversityQingdao266071P.R.China Leibniz Institute for Catalysis e.V. Albert-Einstein-Strasse 29aRostock18059Germany School of Materials Science and Engineering Ocean University of ChinaQingdao266100P.R.China Shenzhen Key Laboratory of Micro/Nano-Porous Functional Materials(SKLPM) SUSTech-Kyoto University Advanced Energy Materials Joint Innovation Laboratory(SKAEM-JIL)Key University Laboratory of Highly Efficient Utilization of Solar Energy and Sustainable Development of GuangdongDepartment of Chemistry and Department of Materials Science and EngineeringSouthern University of Science and Technology(SUSTech)Shenzhen518055P.R.China

PtCo nanoalloys(NAs)deposited on carbon black are emerging as robust electrocatalysts for addressing the sluggish kinetic issue of oxygen reduction reaction(ORR).However,developing a simple and low-cost method to synthesize PtCo/C with excellent performance is still a great *** this work,a one-pot method was used to successfully obtain the PtCo NAs on commercial carbon supports of acetylene black and Ketjenblack ECP600JD,*** with those grown on Ketjenblack ECP600JD,the PtCo NAs grown on acetylene black exhibited higher electrochemical surface area(ECSA)and mass activity(MA),which may be attributed to the different particle sizes of PtCo NAs,distinct hydrophilicity,electroconductivity and charge distribution between the carbon supports and PtCo *** study provides valuable insights into the optimal design of carbon-supported ORR electrocatalysts with exceptional activity and durability.

关键词： Oxygen reduction reaction PtCo nanoalloy Carbon-supported nanostructure Acetylene black

Frequency-stabilized diode laser at 780 nm with a continuously locked time over 100 h

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Chinese Optics Letters 2010年第5期8卷 496-498页

作者：周通齐向晖王青熊炜段俊周小计陈徐宗 Institute of Quantum Electronics School of Electronics Engineering and Computer SciencePeking University

Two extended-cavity diode lasers at 780 nm which are longtime frequency-stabilized to Rb^（87） saturated absorption signals are reported.A high-performance frequency-locking circuit module using a first-harmonic detection technique is designed and *** lasers are continuously frequency-stabilized for over 100 h in conventional laboratory *** Allan standard deviation of either laser is estimated to be 1.3×10^（-11） at an integration time of 25 *** system environment temperature drift is demonstrated to be the main factor affecting long-term stability of the stabilized lasers based on our correlation study between beat frequency and system environment temperature.

关键词： Rubidium

开菲尔粒中菌种微囊化条件的优化和发酵特性研究

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中国食品学报 2017年第5期17卷 73-81页

作者：王亮刘克营刘朋龙齐向辉江苏大学食品与生物工程学院江苏镇江212013 新疆天润乳业股份有限公司乌鲁木齐830000

本文研究了开菲尔粒菌种液芯微囊化的制备工艺,菌种的包埋率,并对菌种包埋后发酵效果进行研究。通过评估海藻酸钠、黄原胶、氯化钙添加浓度和搅拌速度对开菲尔粒分离菌种包埋率和微囊收获数的影响,选出最佳的包埋参数范围。根据Box-Benh... 详细信息

本文研究了开菲尔粒菌种液芯微囊化的制备工艺,菌种的包埋率,并对菌种包埋后发酵效果进行研究。通过评估海藻酸钠、黄原胶、氯化钙添加浓度和搅拌速度对开菲尔粒分离菌种包埋率和微囊收获数的影响,选出最佳的包埋参数范围。根据Box-Benhnken Design试验设计,建立包埋率与因素变量的二次回归模型方程,并经验证该模型回归显著。最佳包埋条件是:海藻酸钠质量分数0.6%,黄原胶质量分数0.4%,氯化钙质量分数5%,搅拌速度700 r/min,此时最大包埋率89.13%。与理论预测值89.46%相差0.33%,试验结果可靠。经试验比较,液芯微囊化包埋菌种和开菲尔粒原粒同时发酵生产开菲尔,并用GC-MS法测定两种发酵乳的挥发性香气成分,结果主要的香气成分基本相同,分别是酯、醇和羰基化合物。这说明利用液芯微囊化包埋开菲尔菌种可行性高,为开菲尔产业的发展提供了研究依据。

关键词：开菲尔粒微囊化包埋率响应面分析香气成分

Advance in Protein Design

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Advance in Protein Design