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Low phosphorus promotes NSP1–NSP2 heterodimerization to enhance strigolactone biosynthesis and regulate shoot and root architecture in rice

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Molecular Plant 2023年第11期16卷 1811-1831页

作者： Kun Yuan Hao Zhang Chaoji Yu Nan Luo Jijun Yan Shuang Zheng Qingliang Hu Dahan Zhang Liquan Kou xiangbing Meng Yanhui Jing Mingjiang Chen Xinwei Ban Zongyun Yan Zefu Lu Jian Wu Yu Zhao Yan Liang Yonghong Wang Guosheng Xiong Jinfang Chu Ertao Wang Jiayang Li Bing Wang State Key Laboratory of Plant Genomics and National Center for Plant Gene Research(Beijing) Institute of Genetics and Developmental BiologyInnovation Academy for Seed DesignChinese Academy of SciencesBeijing 100101China University of Chinese Academy of Sciences Beijing 100049China National Key Laboratory of Plant Molecular Genetics CAS Center for Excellence in Molecular Plant SciencesInstitute of Plant Physiology and EcologySIBSChinese Academy of SciencesShanghai 200032China State Key Laboratory of Crop Gene Resources and Breeding Institute of Crop SciencesChinese Academy of Agricultural SciencesBeijing 100081China Guangdong Laboratory for Lingnan Modern Agriculture State Key Laboratory for Conservation and Utilization of Subtropical Agro-BioresourcesCollege of AgricultureSouth China Agricultural UniversityGuangzhou 510642China National Key Laboratory of Crop Genetic Improvement Hubei Hongshan LaboratoryHuazhong Agricultural UniversityWuhan 430070China College of Life Sciences Shandong Agriculture UniversityTai’anShandong 271018China Academy for Advanced Interdisciplinary Studies Nanjing Agricultural UniversityNanjing 210095China Yazhouwan National Laboratory SanyaHainan 572024China

Phosphorus is an essential macronutrient for plant development and metabolism,and plants have evolved ingenious mechanisms to overcome phosphate(Pi)***,the molecular mechanisms underlying the regulation of shoot and root architecture by low phosphorus conditions and the coordinated utilization of Pi and nitrogen remain largely ***,we show that Nodulation Signaling Pathway 1(NSP1)and NSP2 regulate rice tiller number by promoting the biosynthesis of strigolactones(SLs),a class of phytohormones with fundamental effects on plant architecture and environmental *** found that NSP1 and NSP2 are induced by Oryza sativa PHOSPHATE STARVATION RESPONSE2(OsPHR2)in response to low-Pi stress and form a complex to directly bind the promoters of SL biosynthesis genes,thus markedly increasing SL biosynthesis in ***,the NSP1/2–SL signaling module represses the expression of CROWN ROOTLESS 1(CRL1),a newly identified early SL-responsive gene in roots,to restrain lateral root density under Pi *** also demonstrated that GR24^(4DO) treatment under normal conditions inhibits the expression of OsNRTs and OsAMTs to suppress nitrogen absorption but enhances the expression of OsPTs to promote Pi absorption,thus facilitating the balance between nitrogen and phosphorus uptake in ***,we found that NSP1p:NSP1 and NSP2p:NSP2 transgenic plants show improved agronomic traits and grain yield under low-and medium-phosphorus *** together,these results revealed a novel regulatory mechanism of SL biosynthesis and signaling in response to Pi starvation,providing genetic resources for improving plant architecture and nutrient-use efficiency in low-Pi environments.

关键词： rice Pi deficiency strigolactone NSP1 and NSP2 CRL1 nitrogen and Pi absorption

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管理层海外经历、短视主义与企业创新:有调节的中介效应

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科技进步与对策 2022年第19期39卷 78-87页

作者：乔鹏程徐祥兵西藏民族大学财经学院西藏民族大学管理学院陕西咸阳712082

高管海外经历具有知识溢出效应,能够促进企业创新。采用2012—2020年中国A股上市公司数据,实证检验管理层海外经历、短视主义对企业创新的影响机制。结果表明,管理层海外经历通过抑制短视主义促进企业创新,这一影响路径受到融资约束的... 详细信息

高管海外经历具有知识溢出效应,能够促进企业创新。采用2012—2020年中国A股上市公司数据,实证检验管理层海外经历、短视主义对企业创新的影响机制。结果表明,管理层海外经历通过抑制短视主义促进企业创新,这一影响路径受到融资约束的前半路径调节和政府补助的后半路径调节。基于企业生命周期视角,上述影响路径在成长期和成熟期成立,在衰退期不成立,融资约束对上述影响路径的前半部分调节在成熟期成立,政府补助对上述影响路径的后半部分调节在成长期成立。研究结论有助于打开管理层海外经历对企业创新影响机制的“黑箱”,拓展管理层海外经历经济后果研究,为企业创新高质量发展提供经验证据。

关键词：海外经历短视主义企业创新融资约束政府补助

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Effects of soybean raffinose on growth performance,digestibility,humoral immunity and intestinal morphology of growing pigs

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Animal Nutrition 2021年第2期7卷 393-399页

作者： Zhu Zeng Yalin Zhang Jun He Jie Yu xiangbing Mao Ping Zheng Yuheng Luo Junqiu Luo Zhiqing Huang Bing Yu Daiwen Chen Animal Nutrition Institute Sichuan Agricultural UniversityYa'an625014SichuanChina Key Laboratory of Animal Disease-Resistance Nutrition Ministry of EducationYa'an625014SichuanChina

There are appreciable does of raffinose in soybean,but the impacts of raffinose on pigs are poorly *** used 2 experiments to investigate the influence of soybean raffinose on growth per-formance,digestibility,humoral immunity and intestinal morphology of growing *** Exp.1,a total of 30 crossbred(Duroc×Landrace×Yorkshire)barrows(21.93±0.43 kg)were randomly divided into 3 groups,and were fed with the control diet,the control diets supplemented with 0.2%and 0.5%raffinose,respectively,for 21 *** showed that the addition of 0.2%or 0.5%raffinose reduced(P<0.05)average daily feed intake(ADFI),average daily gain(ADG)and nutrient digestibility,and dietary 0.5%raffinose increased the ratio of feed to gain(P<0.05).For serum indexes,dietary 0.5%raffinose decreased growth hormone and increased glucagon-like peptide-2,immunoglobulin G,tumor necrosis factor-α(TNF-α)and interleukin-6 concentration(P<0.05).In Exp.2,a total of 24 crossbred barrows(38.41±0.45 kg)were randomly divided into 3 groups,and were fed with the control diet(ad libitum),the raffinose diet(0.5%raffinose,ad libitum),and the control diet in the same amount as the raffinose group(feed-pair group)for 14 d,*** with the control diet,dietary 0.5%raffinose decreased ADFI(P<0.05).Intriguingly,the raffinose group had lower ADG than the feed-pair group,lower nutrient digestibility,lower amylase activity in duodenum,lower amylase,lipase and trypsin ac-tivities in jejunum and higher TNF-αconcentration in serum compared with the other 2 groups,and a higher ratio of villus height to crypt depth compared with the control group(P<0.05).These results showed that soybean raffinose could reduce feed voluntary intake and body gain while improving in-testinal morphology without a significant negative influence on *** together,dietary raffinose could decrease growth performance by reducing both feed intake and nutrient digestibility while inducing humoral immune response of growing pi

关键词： Soybean raffinose Growth performance Nutrient digestibility Humoral immunity Pig

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基于AI技术数据库查询优化的研究

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安徽职业技术学院学报 2023年第2期22卷 15-20,26页

作者：尹向兵安徽警官职业技术学院教务处安徽合肥230031

数据查询优化是数据库中最具有挑战性的问题之一,基数估计(CE)是数据库查询优化中的一个重要组成部分。本文应用基于神经网络的查询基数估计方法完成数据表示、模型构建,通过对比实验表明,与现有的基于深度学习算法相比,文中提出的基于... 详细信息

数据查询优化是数据库中最具有挑战性的问题之一,基数估计(CE)是数据库查询优化中的一个重要组成部分。本文应用基于神经网络的查询基数估计方法完成数据表示、模型构建,通过对比实验表明,与现有的基于深度学习算法相比,文中提出的基于图神经网络的数据库查询优化方法,相较于传统的机器学习算法和基于卷积的深度学习算法,查询基数估计误差降低了30%。

关键词：数据库查询优化图神经网络基数估计

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Leucine modulates the IPEC-J2 cell proteome associated with cell proliferation, metabolism and phagocytosis

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Animal Nutrition 2018年第3期4卷 316-321页

作者： xiangbing Mao Man Ren Daiwen Chen Bing Yu Lianqiang Che Jun He Junqiu Luo Yuheng Luo Jianping Wang Hui Sun Animal Nutrition Institute Sichuan Agricultural University Animal Science Collage Anhui Science and Technology University College of Animal Science and Technology Jilin Agricultural University

Leucine can affect intestinal protein expressions, and improve mucosal immune function. However, little study has been conducted to determine the change of protein component by leucine treatment in intestine epithelial cells. The present study was to cover the key proteins and cell pathways that could be regulated by leucine treatment in porcine intestinal epithelial cell line(IPEC-J2) cells with the approach of proteome analysis. A total number of 3,211 proteins were identified in our approach by searching the database of Uniprot sus scrofa. Among identified proteins, there were 101 proteins expressed differently between control group and leucine group. Compared with the control group, there were 50 up-regulated proteins and 51 down-regulated proteins in leucine group. In these proteins, leucine treatment decreased the expression of some proteins including pyruvate kinase, glyceraldehyde-3-phosphate dehydrogenase,E3 ubiquitin ligase, cathepsin D, caspase 3 and caspase 6, and increased the levels of some proteins, such as some eukaryotic translation initiation factors, ubiquitin carboxyl-terminal hydrolase, DNA-related RNA polymerase II, urokinase plasminogen activator, cyclin-dependent kinase inhibitor 2 b, Mut L homolog 1,5-methylcytosine binding domain 4, polymerase d, a-tubulin, syntaxin 18, Ras homolog D, actin related protein 2/3 complex and cofilin. Via the analysis of Gene Ontology and pathways, these proteins in IPECJ2 cells were related with some physiological functions, such as protein metabolism, glycolysis, cell proliferation, apoptosis and phagocytosis. Thus, these results suggest that leucine affects gut barrier function possibly via regulating cell proliferation and apoptosis, metabolism and phagocytosis.

关键词： Leucine IPEC-J2 cells Proteome Phagocytosis Cell proliferation Apoptosis and metabolism

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Effect of different dietary protein levels and amino acids supplementation patterns on growth performance, carcass characteristics and nitrogen excretion in growing-finishing pigs

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Journal of Animal Science and Biotechnology 2020年第1期11卷 111-120页

作者： Yumei Zhao Gang Tian Daiwen Chen Ping Zheng Jie Yu Jun He xiangbing Mao Zhiqing Huang Yuheng Luo Junqiu Luo Bing Yu Key Laboratory of Animal Disease-Resistance Nutrition Animal Nutrition InstituteSichuan Agricultural University46#Xinkang RoadYucheng DistrictYaan 625014SichuanChina

Background:This study was conducted to determine the effects of different dietary protein levels and amino acids supplementation patterns in low protein diets on the growth performance,carcass characteristics and nitrogen excretion in growing-finishing ***-two barrows(25.00±0.39 kg)were randomly assigned to 7 *** 1:the high crude protein diet with balanced for 10 essential amino acids(EAAs).Diet 2:the medium crude protein diet with 2%(approx)decreased protein level of Diet 1 and balanced 10 *** 3:the low crude protein diet with 4%decreased protein level of Diet 1 and balanced 10 *** protein levels of Diet 4,5,6 and 7 were the same as that of Diet *** 4 was only balanced for lysine(Lys),methionine(Met),threonine(Thr)and tryptophan(Trp);Diet 5 and 6 were further supplemented with extra isoleucine(Ile)or valine(Val),respectively;Diet 7 was further supplemented with extra Ile+***:Over the 112 days trial,the reduction of dietary protein by 2%or 4%with balanced10 EAAs significantly decreased nitrogen excretion(P0.05).In low protein diet,Val supplementation significantly increased body weight gain at 25–50 kg phase(P0.05).The total N excretion of pigs supplemented with only Lys,Met,Thr and Trp was numerically higher than that of pigs fed with extra Ile,or Val,or Ile+Val ***:In low protein diet,Val is more required than Ile in the early growing phage(25–50 kg),while Ile becomes more required in the late growing and finishing phage(75–125 kg).

关键词： Growing-finishing pigs Growth performance Isoleucine Low protein diets Nitrogen excretion Valine

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MONOCULM 3,an Ortholog of WUSCHEL in Rice,Is Required for Tiller Bud Formation

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Journal of Genetics and Genomics 2015年第2期42卷 71-78页

作者： Zefu Lu Gaoneng Shao Jinsong Xiong Yongqing Jiao Jing Wang Guifu Liu xiangbing Meng Yan Liang Guosheng Xiong Yonghong Wang Jiayang Li State Key Laboratory of Plant Genomics and National Center for Plant Gene Research (Beijing) Institute of Genetics and Developmental BiologyChinese Academy of Sciences College of Horticulture Nanjing Agricultural University Oil Crops Research Institute China Academy of Agricultural Science Rice Research Institute Sichuan Agricultural University Agricultural Genome Institute China Academy of Agricultural Science

WUSCHEL （WUS） plays an essential role for the maintenance of meristem activity in dicots, but its function is still elusive in monocots. We isolated a new monoculm mutant, monoculm 3 （moc3）, in which a point mutation causes the premature termination of rice O. sativa WUS （OsWUS）. Morphological observation revealed that the formation of tiller buds was disrupted in moc3. MOC3 was localized in the nuclear and could interact with TOPLESS-RELATED PROTEINS （TPRs）. The expression of MOC3 was induced by cytokinins and defection of MOC3 affected the expression of several two-component cytokinin response regulators, OsRRs and ORRs. Our results suggest that MOC3 is required for the formation of axillary buds and has a complex relationship with cytokinins.

关键词： MOC3 WUSCHEL Tiller bud formation Cytokinins Oryza sativa L

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Inhibition of adipogenic differentiation by myostatin is alleviated by arginine supplementation in porcine-muscle-derived mesenchymal stem cells

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Science China(Life Sciences) 2011年第11期54卷 908-916页

作者： LEI HuLong YU Bing YANG XueRong LIU ZeHui HUANG ZhiQing MAO xiangbing TIAN Gang HE Jun HAN GuoQuan CHEN Hong MAO Qian CHEN DaiWen Key Laboratory for Animal Disease-Resistance Nutrition of Ministry of Education of China Animal Nutrition Institute Sichuan Agricultural University Ya＇an 625014 China

Porcine mesenchymal stem cells in postnatal muscle have been demonstrated to differentiate into *** increases adipocyte number and lipid accumulation,and is thought to be the origin of intramuscular *** this study,the effects of myostatin and arginine on adipogenic differentiation in mesenchymal stem cells derived from porcine muscle (pMDSCs) were investigated in *** triglyceride levels were reduced by exogenous myostatin and increased by arginine supplementation or myostatin antibody (P<0.01).The inhibition of lipid accumulation by myostatin in pMDSCs was alleviated by arginine supplementation (P<0.01).Expression patterns of adipogenic transcription factors showed that exogenous myostatin suppressed PPARγ2 and aP2 expression (P<0.01),while supplemental arginine or myostatin antibody promoted ADD1 expression (P<0.01).Furthermore,compared with the addition of either myostatin protein or antibody alone,ADD1 and PPARδ expression were promoted by the combination of arginine and myostatin (P<0.01),and arginine combined with myostatin antibody promoted the expression of ADD1,PPAR,C/EBP,PPAR 2 and LPL in pMDSCs (P<0.05).These results suggest that myostatin inhibits adipogenesis in pMDSCs,and that this can be alleviated by arginine supplementation,at least in part,through promoting ADD1 and PPAR expression.

关键词：肌肉生长抑制素脂肪细胞分化间充质干细胞精氨酸猪肌肉 ADD1 PPAR 肌内脂肪

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菠萝蜜种子淀粉可生物降解复合膜的制备及性能

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复合材料学报 2022年第6期39卷 2907-2917页

作者：王向兵李小侠冯航航彭辉马国富西北师范大学化学化工学院生态功能高分子材料教育部重点实验室甘肃省高分子材料重点实验室兰州730070

针对传统塑料制品难降解、污染环境等问题,选用环境友好、可降解菠萝蜜种子淀粉(JFss)、羧甲基纤维素钠(CMC)和海藻酸钠(SA)为原料,采用涂膜工艺制备了一种可生物降解复合膜。研究JFss的用量对复合膜力学性能、耐水性、水溶性、透湿性... 详细信息

针对传统塑料制品难降解、污染环境等问题,选用环境友好、可降解菠萝蜜种子淀粉(JFss)、羧甲基纤维素钠(CMC)和海藻酸钠(SA)为原料,采用涂膜工艺制备了一种可生物降解复合膜。研究JFss的用量对复合膜力学性能、耐水性、水溶性、透湿性的影响及复合膜润湿性随着时间变化规律,并对复合膜进行土埋降解性测试。采用SEM、FITR、XRD和TGA对复合膜形貌、结构和热稳定性表征。结果表明,JFss的添加使复合膜拉伸强度提高35.8%,耐水性提高4.16%,水溶性提高7.8%,水蒸汽阻隔性提高153.7%,且具有良好的润湿性、保湿性和生物降解性。另外,复合膜中CMC、SA、JFss各组分形成分子间氢键,具有良好的相容性和热稳定性。本方法复合膜制备的原料廉价、制备简单,可大规模生产,在生物降解材料领域具有潜在的应用价值。

关键词：菠萝蜜种子淀粉羧甲基纤维素钠海藻酸钠可生物降解复合膜相容性

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The Determinant Factors of Board Leadership Structure: Working Ability or Succession Plan——Evidence from Chinese Listed Companies 3rd

The Determinant Factors of Board Leadership Structure: Worki...

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2011 International Conference on Information Management and Engineering(ICIME 2011)

作者： Xie xiangbing Accounting Institute Henan University of Economics and Low HUEL

ISBN: (纸本)9781612842387

This paper studies the determinant factors of board leadership structure by using the data from the listed companies in China. The study finds that the chief executive officer (CEO) succession plan hypothesis is significantly negative related with the board leadership structure. However, the variables that reflect the working ability of CEO cannot be through empirical test. This paper also finds that compared with non-SOE, SOES are more likely to have a single board leadership structure. This paper explains the differences in the board leadership structure in China’s listed companies, and provides the new empirical evidence that the board leadership structure does not have a fixed pattern for all the companies.

关键词： leadership structure working ability succession plan determinant factors

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