检索结果-南通市图书馆

MMP-1 Over-expression Promotes Malignancy and Stem-Like Properties of Human Osteosarcoma MG-63 Cells In Vitro

Current Medical Science 2018年第5期38卷 809-817页

作者： Man-li TANG xiang-jun bai Yong LI Xiao-jing DAI Fan YANG Department of Traumatic Surgery Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China Department of Orthopaedic Surgery McGovern Medical Schoolthe University of Texas Health Science Center at Houston (UTHealth)Houston77035USA Center for Stem Cell and Regenerative Medicine UTHealthHouston 77035USA Center for Regenerative Sports Medicine Steadman Philippon Research InstituteFail 81657USA Department of Pharmacology and Toxicology College of Pharmacythe University of HoustonHouston 77555USA

Osteosarcoma is the most common primary malignant bone tumor in childhood,and it maintains a high level of *** metalloproteinase-1(MMP-1)was found to contribute to cancer *** present study was to investigate the in vitro effects of MMP-1 over-expression on the proliferation,invasion,metastasis and stem-like properties of osteosarcoma MG-63 *** MG-63 cells were cultured and had a full length MMP-1 cDNA inserted by the tentiviral vector (MG-63^MMP-1+).MG-63 negative control and MG-63 blank control groups were established as ***-1 expression was detected in MG-63^MMP-1+,MG-63 negative control and MG-63 blank control cells using qPCR,Western blotting and immunofluorescence after 24h of culture. The cell proliferation assay was performed with a camera attached to a bioreactor,which was programmed to photograph five regions of each well every 10 min over a period of 48 *** cell invasion assay was conducted with Matrigel to assess the invasive potential,of MG-63 cells over 24h,the qPCR analysis to measure stem cell markers,including Oct4, Sox-2,Nanog,and Pax-7,and Western blot analysis to detect invasive and metastatic potential markers TIMP-1,VEGF and BMP2/4,after 24h of *** was used to investigate the presence of the stem cell marker Pax-7 after 24-h culture. The results showed that over-expression of MMP-1 after transfection could significantly increase minor cell proliferation and invasion (P<0.05,MG-63^MMP-1+ versus controls).Pax-7 was highly expressed in MG-63^MMP-1+ cells,with no significant changes of Oct-4,Sox-2, and Nanog observed (P<0.05).MG-63^MMP-1+ cells showed higher expression of VEGF and BMP 2/4 proteins and lower expression of TIMP-1 protein than controls (P<0.05).It was concluded that MMP-1 over-expression in MG-63 cells contributed to the proliferation, invasion,metastasis and stem-like properties of osteosarcoma *** studies should focus on in vivo effects of MMP-1 over-expression an

关键词： osteosarcoma MG-63 cells matrix metalloproteinase-1 proliferation invasion metastasis

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Cerium enrichment on sinter skin of mischmetal doped WC-Co cemented carbide

引用

Journal of Central South University of Technology 2005年第Z1期12卷 5-8页

作者： ZHANG Li CHEN Shu XIONG xiang-jun HUANG bai-yun ZHANG Chuan-fu State Key Laboratory of Powder Metallurgy Central South University Changsha 410083 China Changsha Mining and Metallurgy Research Institute Changsha 410012 China State Key Laboratory of Powder Metallurgy Central South University Changsha 410083 China State Key Laboratory of Powder Metallurgy Central South University Changsha 410083 China School of Metallurgical Science and Engineering Central South University Changsha 410083 China

Rare earth-doped WC-Co cemented carbides with low carbon-containing level were prepared by adding mischmetal (mainly containing lanthanum and cerium) in the form of cobalt pre-alloyed powder during *** sintering, the sinter skin (exterior) was observed and analyzed by scanning electron microscopy(SEM)and energy disperse X-ray spectroscopy(EDXS). The results show that during the sintering process lanthanum and cerium are separated and cerium predominantly migrates towards the surface forming precipitates containing *** analysis and polarization of cerium atom caused by the strong interaction between cerium atoms and high energy carbon atoms were used in explaining the observed phenomenon.

关键词： hardmetal lanthanum cerium atom polarization thermoldynamics enrichment

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Cross-sectional study of the educational background and trauma knowledge of trainees in the “China trauma care training” program

引用

Military Medical Research 2020年第1期7卷 44-54页

作者： Hao Tang Dong Liu Dong Yang Jia-Xin Tan Xiu-Zhu Zhang xiang-jun bai Mao Zhang Lian-Yang Zhang Trauma Center State Key Laboratory of TraumaBurns and Combined InjuryDaping HospitalArmy Medical UniversityChongqing 400042China Department of Traumatic Surgery Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China Department of Emergency Medicine the Second Affiliated Hospital of Medical CollegeZhejiang UniversityHangzhou 310009China

Background:Since the trauma knowledge of trauma providers correlates with the outcomes of injured patients,this study aims to assess the socio-demographic characteristics and levels of trauma knowledge of trainees in the China trauma care training(CTCT)program in addition to their post-course test results to provide support for the development of trauma care training programs and trauma systems in ***:A cross-sectional study was conducted by collecting demographic information,hospital-related information and trauma knowledge of the trainees from 19 regions in *** participants were assessed by questionnaires collecting the socio-demographic data,the trauma care knowledge levels and the information of the ***:There were 955 males(78.9%)and 256 females(21.1%)*** them,854 were physicians(70.5%),357 were registered nurses(29.5%).In addition,64 of them also played an administrative role in the hospitals(5.3%).The score of the trainees who were members of the emergency department staff(72.59±14.13)was the highest among the scores of all the personnel surveyed,followed by those of the trainees from the intensive care unit(ICU)(71.17±12.72),trauma surgery department(67.26±13.81),orthopedics department(70.36±14.48),general surgery department(69.91±14.79)and other departments(69.93±16.91),P=*** score of the professors(73.09±15.05)was higher than those of the associate professors(72.40±14.71),lecturers(70.07±14.25)and teaching assistants(67.58±15.16),P<*** score of the individuals who attended experts’trauma lectures(72.22±14.45)was higher than that of individuals who did not attend the lectures(69.33±15.17),P=*** mean scores before and after the training were 71.02±14.82 and 84.24±13.77,respectively,P<*** mean score of trauma knowledge after the training of trainees from different provinces and with different educational backgrounds was higher than that before the training,with a statistically significant differ

关键词： Cross-sectional study Trauma care knowledge Educational background Continuing medical education

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Trauma care system in China

引用

Chinese Journal of Traumatology 2018年第2期21卷 80-83页

作者： Tao Liu xiang-jun bai Department of Traumatic Surgery Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan China

With the development of modern society, high-energy trauma has become an increasing tendency, which brings a great challenge for trauma care. A well-running trauma care system that is composed by pre-hospital and in-hospital care has been proved to decrease the death and disability rate of trauma patients. However, establishment of trauma care system in China is still at the initial stage. Trauma care systems in China and developed countries represented by the United States and Germany are introduced respectively in this article. Construction of regional and hierarchical trauma center, training of specific trauma care team and performance of integrative trauma rescue model are recommended in China.

关键词： Trauma care system Trauma center Integrative mode

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Effect of triptolide on secretion of inflammatory cellular factors TNF-α and IL-8 in peritoneal macrophages of mice activated by lipopolysaccharide

引用

World Journal of Emergency Medicine 2010年第1期1卷 70-74页

作者： Fan Yang xiang-jun bai Duan Hu Zhan-fci Li Kai-jun Liu Department of Traumatic Surgery Affiliated Tongji hospital Tongji Medical College Huazhong University of Science andTechnology Wuhan 430030 China

Research has been carried out to look for safe and effective anti-inflammation drugs from traditional Chinese herbal medicine. As a powerful research technology of life science, molecular biology has entered many areas of traditional Chinese *** study aimed to investigate the effect of triptolide on tumor necrosis factor-α （TNF-α） and interleukin-8 （IL-8） of peritoneal macrophages activated by lipopolysaccharide （LPS） in mice. Peritoneal elicited macrophages were separated, purified and activated by LPS in mice, then cultured in vitro with triptolide at different concentrations. The activity of TNF-a and the level of IL-8 of cellular supernatants were determined by MTT colorimetric assay and ELISA, respectively. The activity of TNF-a in macrophages was significantly inhibited （P〈0.01） by triptolide （10^-1-10^1μg/ml） during 4-24 hours in a time- and dose-dependent manner. The level of IL-8 in macrophages was significantly inhibited （P〈0.01） by triptolide （10^-1-10^1g/ml） in 12 hours in a dose- dependent manner. Triptolide could inhibit the activity of TNF-a and the level of IL-8 in macrophages activated by LPS.

关键词： Triptolide Lipopolysaccharide Macrophage Tumor necrosis factor-α Interleukin-8

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Correlations between quality indexes of chest compression

引用

World Journal of Emergency Medicine 2013年第1期4卷 54-58页

作者： Feng-ling Zhang Li Yan Su-fang Huang xiang-jun bai Department of Emergency Medicine Tongji HospitalTongji Medical CollegeHuazhong University of Science and Technology

BACKGROUND:Cardiopulmonary resuscitation(CPR) is a kind of emergency treatment for cardiopulmonary arrest,and chest compression is the most important and necessary part of *** American Heart Association published the new Guidelines for Cardiopulmonary Resuscitation and Emergency Cardiovascular Care in 2010 and demanded for better performance of chest compression practice,especially in compression depth and *** current study was to explore the relationship of quality indexes of chest compression and to identify the key points in chest compression training and ***:Totally 219 healthcare workers accepted chest compression training by using Laerdal ACLS advanced life support resuscitation *** quality indexes of chest compression,including compression hands placement,compression rate,compression depth,and chest wall recoil as well as self-reported fatigue time were monitored by the Laerdal Computer Skills and Reporting ***:The quality of chest compression was related to the gender of the *** indexes in males,including self-reported fatigue time,the accuracy of compression depth and the compression rate,the accuracy of compression rate,were higher than those in ***,the accuracy of chest recoil was higher in females than in *** quality indexes of chest compression were correlated with each *** self-reported fatigue time was related to all the indexes except the compression ***:It is necessary to offer CPR training courses *** clinical practice,it might be better to change the practitioner before fatigue,especially for females or weak *** training projects,more attention should be paid to the control of compression rate,in order to delay the fatigue,guarantee enough compression depth and improve the quality of chest compression.

关键词： Cardiopulmonary resuscitation Chest compression Quality indexes Correlation

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Early intramedullary nailing for femoral fractures in patients with severe thoracic trauma： A systemic review and meta-analysis

引用

Chinese Journal of Traumatology 2016年第3期19卷 160-163页

作者： Xiao-Yuan Liu Meng Jiang Cheng-LaYi xiang-jun bai David J. Hak Department of Orthopaedic Surgery Daye People's Hospital Daye 435100 China Department of Trauma Surgery Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430030 China Department of Orthopaedic Surgery. Denver Health Medical Center University of Colorado Denver. School of Medicine Denver 80204 USA

Purpose： Early intramedullary nailing （IMN） within the first 24 h for multiply injured patients with femoral fracture and concomitant thoracic trauma is controversial. Previously published studies have been limited in size and their outcomes have been inconclusive. A meta-analysis was conducted to evaluate the available data in order to guide care and help improve the outcomes for these patients. Methods： We searched the literature up to December 2011 in the main medical search engines and identified 6 retrospective cohort studies that explored the safety of early IMN in patients with both femoral fracture and chest injury. Our primary outcome was the rates of pulmonary complication （pneumonia, adult respiratory distress syndrome, fat embolism syndrome）, multiple organ failure （MOF） and mortality. Results： We found no statistically significant difference in the rate of pulmonary complications, MOF or mortality in the patients treated with early IMN. Conclusion： Early 1MN for femoral fractures does not increase the mortality and morbidity in chest- iniured patients in the studies anah/zed.

关键词： Thoracic injuriesFemoral fracturesFracture fixationlntramedullaryMeta-analysis

来源：

维普期刊数据库

同方期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Engineering N3C vacancies in hierarchical porous carbon nitride nanosheets for room temperature ultradeep photocatalytic aerobic oxidative desulfurization

引用

Science China Chemistry 2025年

作者： Yina Nie Weijie Yang Xin Wang xiang Li Lele Wei Xiaolin bai Lin Liu Feng Fu jun Wan College of Chemistry & Chemical Engineering Research Institute of Comprehensive Energy Industrial Technology Shaanxi Key Laboratory of Chemical Reaction Engineering Yan'an University Anhui Engineering Research Center of Carbon Neutrality College of Chemistry and Materials Science Anhui Normal University

The production of ultra-low sulfur clean fuels holds global significance for improving energy efficiency and reducing environmental pollution. Photocatalytic aerobic oxidative desulfurization（PODS） offers an attractive approach to efficiently remove refractory heterocyclic aromatic sulfur compounds under mild conditions. However, the sluggish charge dynamics,insufficient molecule activation, and slow mass transfer within photocatalysts restrict the overall desulfurization efficiency,hindering the widespread application of this technology. Herein, we developed a defective g-C3N4（M1U3CN） photocatalyst with bridged N3Cvacancies and hierarchical porous structures to address these limitations. This catalyst not only demonstrates outstanding dibenzothiophene（DBT） removal efficiency of 99.6% for model oil with high conversion and selectivity but also achieves complete sulfur removal from gasoline as well as 96.7% sulfur removal for distilled diesel at room temperature,successfully achieving ultradeep aerobic oxidative desulfurization. The introduction of rich N3Cvacancies and abundant micromeso-macroporous distribution in M1U3CN enhances kinetic efficiencies by promoting charge carrier separation, mass transfer,and molecular activation simultaneously; resulting in exceptional PODS performance. This work provides a collaborative strategy to overcome kinetic challenges in aerobic oxidative desulfurization via defect and structural engineering of the catalyst.

关键词：

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Current trauma care system and trauma care training in China

引用

Chinese Journal of Traumatology 2018年第2期21卷 73-76页

作者： Lian-Yang Zhang Xiu-Zhu Zhang xiang-jun bai Mao Zhang Xiao-Gang Zhao Yong-An Xu Hao Tan Yang Li Trauma Center. State Key Laboratory of Trauma Burns and Combined Injury Research Institute of Surgery Doping Hospital Amry Medical University Chongqing 400042 China Department of Traumatic Surgery TonEi Hospital Tongji Medical College Huazhong University of Sdence and Technology Wuhon 430030 China Department of Emergency Medidne The Second Affiliated Hospital of Medical College Zhefiang University Zhejiang 310009 China

Trauma is a life-threatening ＂modern disease＂. The outcomes could only be optimized by cost-efficient and prompt trauma care, which embarks on the improvement of essential capacities and conceptual revolution in addition to the disruptive innovation of the trauma care system. According to experiences from the developed countries, systematic trauma care training is the cornerstone of the generalization and the improvement on the trauma care, such as the Advance Trauma Life Support （ATLS）. Currently, the pre-hospital emergency medical services （EMS） has been one of the essential elements of infrastructure of health services in China, which is also fundamental to the trauma care system. Hereby, the China Trauma Care Training （CTCT） with independent intellectual property rights has been initiated and launched by the Chinese Trauma Surgeon Association to extend the up-to-date concepts and techniques in the field of trauma care as well to reinforce the generally well-accepted standardized protocols in the practices. This article reviews the current status of the trauma care system as well as the trauma care training.

关键词： Trauma care system Trauma care training China

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Perilipin-2 mediates ferroptosis in oligodendrocyte progenitor cells and myelin injury after ischemic stroke

引用

Neural Regeneration Research 2025年第7期20卷 2015-2028页

作者： Jian Yang Jiang Wu Xueshun Xie Pengfei Xia Jinxin Lu Jiale Liu Lei bai xiang Li Zhengquan Yu Haiying Li Department of Neurosurgery&Brain and Nerve Research Laboratory The First Affiliated Hospital of Soochow UniversitySuzhouJiangsu ProvinceChina Institute of Stroke Research Soochow UniversitySuzhouJiangsu ProvinceChina

Differentiation of oligodendrocyte progenitor cells into mature myelin-forming oligodendrocytes contributes to *** of remyelination due to oligodendrocyte progenitor cell death can result in severe nerve *** is an iron-dependent form of regulated cell death caused by membrane rupture induced by lipid peroxidation,and plays an important role in the pathological process of ischemic ***,there are few studies on oligodendrocyte progenitor cell *** analyzed transcriptome sequencing data from GEO databases and identified a role of ferroptosis in oligodendrocyte progenitor cell death and myelin injury after cerebral *** analysis suggested that perilipin-2(PLIN2)was involved in oligodendrocyte progenitor cell ***2 is a lipid storage protein and a marker of hypoxia-sensitive lipid droplet *** further investigation,we established a mouse model of cerebral ischemia/*** found significant myelin damage after cerebral ischemia,as well as oligodendrocyte progenitor cell death and increased lipid peroxidation levels around the infarct *** ferroptosis inhibitor,ferrostatin-1,rescued oligodendrocyte progenitor cell death and subsequent myelin *** also found increased PLIN2 levels in the peri-infarct area that co-localized with oligodendrocyte progenitor ***2 knockdown rescued demyelination and improved neurological *** findings suggest that targeting PLIN2 to regulate oligodendrocyte progenitor cell ferroptosis may be a potential therapeutic strategy for rescuing myelin damage after cerebral ischemia.

关键词： bioinformatics bulk RNA sequencing ferroptosis ischemic stroke myelin injury oligodendrocyte progenitor cell perilipin-2 single-cell RNA sequencing

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：