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Antifungal,Insecticidal and Herbicidal Properties of Volatile Components from Paenibacillus polymyxa Strain BMP-11

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Agricultural Sciences in China 2011年第5期10卷 728-736页

作者： ZHAO Li-jing YANG Xiao-nan LI xiang-ying MU wei liu Feng Key Laboratory of Pesticide Toxicology ＆ Application Technique College of Plant Protection Shandong Agricultural University Tai＇an271018 P.R.China

The volatiles produced by Paenibacillus polymyxa strain BMP-11 which was isolated from the rhizosphere soil of cucumber in a greenhouse were analyzed by *** on the preliminary test,three kinds of purchased commercial products 1-octen-3-ol,benzothiazole and citronellol were chosen to give further assessment of their *** bioassays in sealed dishes revealed that those three compounds strongly inhibited the mycelia growth of the eight pathogens at a low treatment dosage and induced the mycelial morphological *** the experiment,we even found that citronellol completely prevented the pigment production of the tested fungus,Fusarium oxysporum,however,1-octen-3-ol and benzothiazole had slight *** germination was inhibited to different degrees when spores of Botrytis cinerea exposed to these compounds for 24 h in water agar ***,fumigation results showed that 1-octen-3-ol and benzothiazole had strong toxicity against Tribolium castaneum,LC50 was 16.76 and 3.50 mg L-1,*** fumigation activity was also found similar to the positive control,1,3-dichloropropene （LC50 =10.13 mg L-1）.Results of herbicidal assays showed that tested compounds had inhibitory effects on the seed germination and seedling growth of Amaranthus retroflexus,Echinochloa crusgalli and Chenopodium *** of the present study suggested that the antifungal,insecticidal and herbicidal properties of the components were contained in *** agents or even their derivatives may have a potential to be used as fungicide,insecticide as well as herbicide.

关键词： volatiles antifungal activity insecticidal activity herbicidal activity

Vanadium(V) Complexes Containing Unsymmetrical N-Heterocyclic Carbene Ligands: Highly Efficient Synthesis and Catalytic Behavior towards Ethylene/Propylene Copolymerization

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Chinese Journal of Polymer Science 2024年第1期42卷 32-41,I0006页

作者： Yi-Cong Wang Hui Zhat Pei-Yi Cheng Shu Zhang xiang-wei liu Yi-Xian Wu State Key Laboratory of Chemical Resource Engineering Beijing University of Chemical TechnologyBejing 100029China

The highly efficient method has been developed for the synthesis of NHC·VOCl_(3) containing symmetrical or unsymmetrical Nheterocyclic carbene(NHC) ligands by the transmetallation reaction of NHC·AgCl with VOCl_(3).The total isolated yield of VOCl_(3)[1,3-(2,4,6-Me_(3)C_(6)H_(2))_(2)(NCH=)_(2)C:](V4') reached 86% by transmetallation reaction,which is much higher than that(48%) by direct coordination *** methodology has also been used to synthesize the novel vanadium complexes containing unsymmetrical NHC ligands of VOCl_(3)[PhCH_(2)NCH=CHNR)C:](V5',R=2,4,6-Me_(3)C_(6)H_(2);V6',R=2,4-Me_(2)-6-Ph-C_(6)H_(2);V7',R=2,6-^(i)Pr_(2)-C_(6)H_(3)) with high yield,which could not be obtained by direct coordination *** catalytic activity and copolymerization ability would be improved by introducing unsymmetrical NHC ligands due to their less steric bulky *** vanadium complex V5' containing unsymmetrical NHC ligand exhibits higher catalytic activity(3.7×10^(5)g_(copolymer)·mol^(-1) of V·h^(-1)) than that of V4' containing symmetrical NHC ***,the higher propylene incorporation ratio(45.6 mol%) in the copolymers of ethylene with propylene could be obtained by using V5' than that(39.9%) by using V4'.The results would provide a highly efficient strategy for the synthesis of early transition metal complexes containing versitile NHC ligands,affording the catalyst with both high catalytic activity and copolymerization ability for the synthesis of high performance polyolefin elastomers.

关键词： Vanadium complex N-heterocyclic carbene ligand Transmetallation reaction Copolymerization of ethylene with propylene

Aression of TLR_9 in human pulmonary adenocarcinoma cell line A549

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The Chinese-German Journal of Clinical Oncology 2009年第7期8卷 393-396页

作者： Jun Yu Tiecheng Pan xiang wei Ligang liu Min Hu Fang Yuan Jiaduo Li Department of Cardiothoracic Surgery Tongji Hospital Tongji Medical College Huazhong University of Science andTechnology Wuahn 430030 China

Objective:Being considered as a bridge between the innate immunity and acquired immunity,Toll-like receptors(TRLs) are very important innate immunity *** researchs on the innate immunity have focused on the relationship between TLRs and human *** paper investgated the expression and significance of TLR9 in human pulmonary adenocarcinoma cell(A549 cell) and human bronchial epithelial cell(HBE cell).Methods:After culturing A549 cell and HBE cell in vitro,the expression of TLR9 mRNA and protein in both cells were detected by immunocytochemistry,Real-time Quantitative Reverse Transcriptase-Polymerase Chain Reaction(Real-time Quantitative PCR) and Western blot,***:By immunocytochemistry staining,TLR9 was mainly expressed in both cells' cell membrane and endochylema as brown-yellow *** showed that the expressions of TLR9 mRNA and protein in A549 cell were stronger than those in HBE cell(P < 0.01).Conclusion:The results suggest TLR9 might cause the progression of human pulmonary adenocarcinoma,and the mechanism needs to be further investgatied.

关键词： TLR9 A549 cell HBE cell immunocytochemistry Real-time Quantitative PCR western blot

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改良手法治疗颈源性眩晕87例

上海中医药杂志 1999年第1期33卷 40-41页

作者：高翔刘伟空军杭州疗养院

用改良手法治疗颈源性眩晕８７例为治疗组，并与７４例单用西比林治疗的对照组进行比较，结果有效率分别为９７７％、７９７３％。改良手法与传统手法比较有四大优点：①取点更准确；②手法简便安全；③因强度小，故反应也小；④注... 详细信息

用改良手法治疗颈源性眩晕８７例为治疗组，并与７４例单用西比林治疗的对照组进行比较，结果有效率分别为９７７％、７９７３％。改良手法与传统手法比较有四大优点：①取点更准确；②手法简便安全；③因强度小，故反应也小；④注重整体调理、筋骨并重。

关键词：颈椎性眩晕推拿手法改良

不同敏感波长轨道不平顺对现代有轨电车运行性能影响分析

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科学技术与工程 2021年第31期21卷 13497-13504页

作者：未向柳许贵阳北京建筑大学机电与车辆工程学院北京100044 北京建筑大学城市轨道交通车辆服役性能保障北京市重点实验室北京100044

为了探究轨道敏感波长对现代有轨电车运行的影响,通过对某独立轮现代有轨电车在不同波长激励时的动力响应指标进行分析,得到电车的敏感波长范围;并以此生成轨道不平顺激励,分析线路在施加此激励前后,车辆通过时参数变化。电车在直线线... 详细信息

为了探究轨道敏感波长对现代有轨电车运行的影响,通过对某独立轮现代有轨电车在不同波长激励时的动力响应指标进行分析,得到电车的敏感波长范围;并以此生成轨道不平顺激励,分析线路在施加此激励前后,车辆通过时参数变化。电车在直线线路运行时,轨向和水平不平顺主要影响横向加速度,高低不平顺主要影响垂向加速度。电车在曲线线路运行时,轨向和水平不平顺分别影响电车的中低速和高速曲线通过性能,两者同时作用时对电车曲线运行具有综合的影响效果,高低不平顺主要改变电车高速曲线运行状态。方向水平逆向不平顺对电车安全性影响会随着波长增大而减小,合理曲线超高能够降低影响,通过分析敏感波长与电车运行的关系,对电车通过不同激励路段形成指导。

关键词：现代有轨电车轨道不平顺有限元动力响应

Expression and Bioinformatics Analysis of SPACA4 in Human and Mice

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Journal of Reproduction and Contraception 2008年第1期19卷 9-15页

作者： Ai-fa TANG Zhen-dong YU Yao-ting GUI Xin GUO Xian-xin LI wei-xiang liu Hui ZHU Zhi-ming CAI Shenzhen Key Lab of Male Reproduction and Genetics Shenzhen Hospital of Peking University Shenzhen 518036 China Research Center of Biomedical Engineering Life Science Division Graduate School at Shenzhen Tsinghua University Shenzhen 518055 China

Objective To analyze the expression of SPACA4 in human and mice. Methods Testes cRNA samples from Balb/c mice of different postnatal days were performed with mouse affymetrix chip to screen the expression of SPACA4 in mice. Sub-quantitative RT-PCR and bioinformatic tools were used here to describe the expression profile of SPACA4 in mice and human. Results The results of gene chip analysis indicated that the expression of mSPACA4 began after d 35 of postnatal testis in mice. Sub-quantitative RT-PCR assay showed that SPACA4 gene expressed exclusively in mouse and human testis, and mouse mSPACA4 gene expressed after d 35 of postnatal testis that was consistency with the results of gene chip analysis. By bioinformatics analysis, mSPACA4 is located in cell membrane （34.8%） or plasma membrane （34.8%）, the signal peptide cleavage site between position 19 and 20 amino acids, transmembrane region between 2-20 and 101-126 amino acids, respectively, on mSPACA4 protein. Conclusion mSPACA4 and hSPACA4 were testis-specific genes, and the expression of mSPACA4 begins after d 35 of postnatal testis in mice. SPACA4 is a candidate for targeting in a sperm-based contraceptive vaccine.

关键词： gene chips SPACA4 gene spermatogenesis male contraceptive vaccine

Investigation of bubbles escape behavior from low basicity mold flux for high-Mn high-Al steels using 3D X-ray microscope

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International Journal of Minerals,Metallurgy and Materials 2025年第1期32卷 102-110页

作者： Qiang liu xiang Li Shen Du Ming Gao Yanbin Yin Jiongming Zhang State Key Laboratory of Advanced Metallurgy University of Science and Technology BeijingBeijing 100083China

During the continuous casting process of high-Mn high-Al steels,various types of gases such as Ar need to escape through the top of the *** which,the behavior of bubbles traversing the liquid slag serves as a restrictive link,closely associated with viscosity and the thickness of liquid *** contrast to two-dimensional surface observation,three-dimensional(3D)analysis method can offer a more intuitive,accurate,and comprehensive ***,this study employs a 3D X-ray microscope(3D-XRM)to obtained spatial distribution and 3D morphological characteristics of residual bubbles in mold flux under different basicity of liquid slag,different temperatures,and different holding *** results indicate that as basicity of slag increases from 0.52 to 1.03,temperature increases from 1423 to 1573 K,the viscosity of slag decreases,the floating rate of bubbles *** addition,when holding time increases from 10 to 30 s,the bubbles floating distance increases,and the volume fraction and average equivalent sphere diameter of the bubbles solidified in the mold flux gradually *** one word,increasing the basicity,temperature,and holding time leading to an increase in the removal rate of bubbles especially for the *** findings of bubbles escape behavior provide valuable insights into optimizing low basicity mold flux for high-Mn high-Al steels.

关键词： mold flux low basicity bubbles three-dimensional X-ray microscope viscosity

Three-dimensional fracture of UO2 ceramic pellets by phase field modeling

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Science China(Physics,Mechanics & Astronomy) 2025年第1期Mechanics & Astronomy) .卷 169-180页

作者： wei Xiong Xuan Ye Hongzhang Cheng Xiaoming liu Institute of Nuclear and New Energy Technology Collaborative Innovation Center of Advanced Nuclear Energy Technology Key Laboratory of Advanced Reactor Engineering and Safety of Ministry of Education Tsinghua University State Key Laboratory of Nonlinear Mechanics Institute of Mechanics Chinese Academy of Sciences School of Engineering Science University of Chinese Academy of Sciences

Fracture is the primary failure type for the UO2ceramic pellets,which are operated under irradiation and thermal processes,and physically confined by the *** this work,by combining closely coupled physical processes of heat conduction,heat transfer,and mechanical deformation with the cohesive phase field framework,we proposed a high-precision modeling of quasistatic cracking of three-dimensional （3D） UO2ceramic fuel pellets under high temperature and *** simulation results agree well with the experimental results,indicating that the proposed method has the potential to capture 3D crack modes in the interested time ***,we studied the relation of the 3D fracture patterns of fuel pellets with the peak power *** was found that the power level plays a critical role in determining the competition between radial and circumferential cracks,as well as transverse penetrating cracks.

关键词： phase field method nuclear fuel pellet coupled thermo-mechanical model thermal fracture

Comparative proteomic analysis of plasma exosomes reveals the functional contribution of N-acetyl-alpha-glucosaminidase to Parkinson’s disease

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Neural Regeneration Research 2025年第10期20卷 2998-3012页

作者： Yuan Zhao Yidan Zhang Xin liu Jian Zhang Ya Gao Shuyue Li Cui Chang xiang liu Guofeng Yang Department of Geriatrics Second Hospital of Hebei Medical UniversityShijiazhuangHebei ProvinceChina Department of Neurosurgery The Second Hospital of Hebei Medical UniversityShijiazhuangHebei ProvinceChina

Parkinson’s disease is the second most common progressive neurodegenerative disorder,and few reliable biomarkers are available to track disease *** proteins,DNA,mRNA,and lipids carried by exosomes reflect intracellular changes,and thus can serve as biomarkers for a variety of *** this study,we investigated alterations in the protein content of plasma exosomes derived from patients with Parkinson’s disease and the potential therapeutic roles of these proteins in Parkinson’s *** a tandem mass tag-based quantitative proteomics approach,we characterized the proteomes of plasma exosomes derived from individual patients,identified exosomal protein signatures specific to patients with Parkinson’s disease,and identified N-acetyl-alpha-glucosaminidase as a differentially expressed protein.N-acetyl-alpha-glucosaminidase expression levels in exosomes from the plasma of patients and healthy controls were validated by enzyme-linked immunosorbent assay and western *** results demonstrated that the exosomal N-acetyl-alpha-glucosaminidase concentration was not only lower in Parkinson’s disease,but also decreased with increasing Hoehn-Yahr stage,suggesting that N-acetyl-alpha-glucosaminidase could be used to rapidly evaluate Parkinson’s disease ***,western blot and immunohistochemistry analysis showed that N-acetyl-alpha-glucosaminidase levels were markedly reduced both in cells treated with 1-methyl-4-phenylpyridinium and cells overexpressingα-synuclein compared with control ***,N-acetyl-alpha-glucosaminidase overexpression significantly increased cell viability and inhibitedα-synuclein expression in 1-methyl-4-phenylpyridinium-treated *** together,our findings demonstrate for the first time that exosomal N-acetyl-alpha-glucosaminidase may serve as a biomarker for Parkinson’s disease diagnosis,and that N-acetyl-alpha-glucosaminidase may reduceα-synuclein expression and 1-methyl-4-phenylpyridinium-induced n

关键词： biomarker diagnosis exosomes N-acetyl-alpha-glucosaminidase Parkinson’s disease proteomic α-synuclein