检索结果-南通市图书馆

缺血性脑卒中铁死亡特征基因NFE2L2的鉴定与验证

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

中国组织工程研究 2025年第7期29卷 1466-1474页

作者：王咪马书杰刘杨齐瑞上海中医药大学附属岳阳中西医结合医院康复医学科上海市200437 上海中医药大学上海市201203 上海市第二康复医院上海市200431

背景:铁死亡与缺血性脑卒中的发病密切相关,靶向铁死亡是一种治疗缺血性脑卒中有前景的方案,但具体调控靶点尚不明确。目的:通过生物信息学和机器学习方法筛选缺血性脑卒中铁死亡相关特征基因,并通过细胞实验进行验证,探讨铁死亡在缺血... 详细信息

背景:铁死亡与缺血性脑卒中的发病密切相关,靶向铁死亡是一种治疗缺血性脑卒中有前景的方案,但具体调控靶点尚不明确。目的:通过生物信息学和机器学习方法筛选缺血性脑卒中铁死亡相关特征基因,并通过细胞实验进行验证,探讨铁死亡在缺血性脑卒中的作用。方法:基于GEO数据库和FerrDb数据库选取符合条件的缺血性脑卒中相关数据集和铁死亡表达数据集,通过t检验筛选铁死亡相关差异基因。对铁死亡相关差异基因进行GO功能富集分析与KEGG信号通路富集分析。通过PPI网络分析和机器学习筛选缺血性脑卒中铁死亡的特征基因,利用ROC分析和GSEA分析探究特征基因的准确性和生物功能。然后进行细胞实验,将HT22细胞分为对照组与缺血性脑卒中组,对照组不作任何干预,缺血性脑卒中组加入0.1 mol/L的H_(2)O_(2)干预24 h诱导细胞氧化应激和铁死亡,通过实时荧光定量RT-PCR和Western Blot验证铁死亡的发生和特征基因表达。结果与结论:(1)共获取45个铁死亡相关差异基因,GO和KEGG富集分析发现差异基因与氧化应激、自噬、铁死亡、脂肪细胞因子信号通路和线粒体代谢密切相关。(2)通过PPI网络中的MCODE插件和cytoHubba插件与机器学习中的LASSO算法和SVM-RFE算法共鉴定出1个铁死亡特征基因核因子E2相关因子2(nuclear factor erythroid 2-related factor 2,NFE2L2)。(3)对NFE2L2进行ROC曲线分析,发现在训练集和验证集中构建的诊断预测模型具有良好的准确性与特异性;对NFE2L2进行GSEA分析,发现特征基因通过免疫、炎症反应、氨基酸代谢及神经因子调控等方面参与缺血性脑卒中发病机制的调控。(4)细胞实验的RT-PCR和Western Blot分析表明,与对照组对比,缺血性脑卒中组中的酰基辅酶A合成酶长链家族成员4 mRNA和蛋白表达水平显著增高(P<0.05),谷胱甘肽过氧化物酶4 mRNA和蛋白表达水平显著降低(P<0.05);与对照组对比,缺血性脑卒中组中特征基因NFE2L2 mRNA和蛋白表达水平显著增高(P<0.05)。(5)上述结果证实,缺血性脑卒中与铁死亡密切相关,靶向特征基因NFE2L2可以为研究和治疗缺血性脑卒中提供一定的思路与方向。

关键词：缺血性脑卒中铁死亡生物信息学 HT22细胞机器学习特征基因细胞实验 NFE2L2 ASCL4 GPX4

在线全文

学校读者我要写书评

暂无评论

核因子I-C调控人根尖牙乳头干细胞的分化

中国组织工程研究 2025年第31期29卷 6667-6673页

作者：吴越朱永娜葛翔刘樊何泽禹刘茜蚌埠医科大学口腔医学院蚌埠医科大学第二附属医院口腔科安徽省蚌埠市233000

背景:体外过表达核因子I-C基因可促进人根尖牙乳头干细胞的分化,Wnt/β-catenin信号通路的激活也可促进人根尖牙乳头干细胞的分化,而且核因子I-C对间充质干细胞中Wnt/β-catenin通路具有调节作用。然而,核因子I-C能否通过激活人根尖牙... 详细信息

背景:体外过表达核因子I-C基因可促进人根尖牙乳头干细胞的分化,Wnt/β-catenin信号通路的激活也可促进人根尖牙乳头干细胞的分化,而且核因子I-C对间充质干细胞中Wnt/β-catenin通路具有调节作用。然而,核因子I-C能否通过激活人根尖牙乳头干细胞中Wnt/β-catenin通路进而影响细胞分化,目前尚未见报道。目的:探讨核因子I-C通过Wnt/β-Catenin信号通路调控人根尖牙乳头干细胞分化的作用。方法:玻片覆盖组织块法培养人根尖牙乳头干细胞,慢病毒转染过表达核因子I-C基因。①设置对照组、空载体组和过表达核因子I-C组,Western blot检测β-Catenin、LRP5和TCF7L2的表达。②设置对照组、空载体组、过表达核因子I-C组和过表达核因子I-C+DKK-1(Wnt通路抑制剂)组,细胞成骨诱导7 d后进行碱性磷酸酶染色及活性定量,细胞成骨诱导14 d后qPCR和Western blot检测Runt相关转录因子2、牙本质涎磷蛋白、骨钙素mRNA及蛋白的表达,茜素红染色观察矿化结节形成情况。结果与结论:①与对照组、空载体组相比,过表达核因子I-C组人根尖牙乳头干细胞中Wnt/β-Catenin通路相关蛋白β-Catenin、LRP5、TCF7L2表达显著升高(P<0.01);②与对照组、空载体组相比,过表达核因子I-C组人根尖牙乳头干细胞中碱性磷酸酶活性显著升高(P<0.01),Runt相关转录因子2、牙本质涎磷蛋白、骨钙素mRNA及蛋白表达均显著升高(P<0.01),矿化结节数量显著增加(P<0.01);③与过表达核因子I-C组相比,过表达核因子I-C+DKK-1组人根尖牙乳头干细胞中碱性磷酸酶活性、Runt相关转录因子2、牙本质涎磷蛋白、骨钙素mRNA及蛋白表达显著下降(P<0.05),矿化结节数量显著减少(P<0.05)。结果表明:核因子I-C能够激活人根尖牙乳头干细胞中Wnt/β-catenin信号通路,介导人根尖牙乳头干细胞的成骨/成牙分化。

关键词：人根尖牙乳头干细胞核因子I-C Wnt 信号通路分化成骨 DKK-1 工程化干细胞

同方期刊数据库

Precipitating thermally reinforcement phase in aluminum alloys for enhanced strength at 400℃

在线全文

学校读者我要写书评

暂无评论

Journal of Materials Science & Technology 2024年第5期172卷 71-82页

作者： xiang Su Yuan Lei Yang Chen Hongjie Qu Zhixiang qi Gong Zheng Xu liu Henggao xiang Guang Chen National Key Laboratory of Advanced Casting Technologies MIIT Key Laboratory of Advanced Metallic and Intermetallic Materials TechnologyEngineering Research Center of Materials Behavior and DesignMinistry of EducationNanjing University of Science and TechnologyNanjing 210094China School of Aviation and Mechanical Engineering Changzhou Institute of TechnologyChangzhou 213032China

Heat-resistant aluminum alloys are widely used in aerospace and automotive fields for manufacturing hot components due to their advantages in lightweight design and energy ***,the high-temperature strength of existing cast aluminum alloys is always limited to about 100 MPa at 350℃due to coarsening and transformation of strengthening ***,we reveal that the yield strength and ultimate tensile strength of the T6 state Al-8.4Cu-2.3Ce-1.0Mn-0.5Ni-0.2Zr alloy at 400◦C increase by 34%and 44%after re-aging at 300℃for 100 h,and its thermal strength exhibits distinguished ad-vantage over traditional heat-resistant aluminum *** enhanced elevated-temperature strength is attributed to the reprecipitation of the Ni-bearing T-Al_(20)Cu_(2)Mn_(3)phase,whose number density increases over one *** significant segregation of Ni,Ce,and Zr elements at the interfaces helps improve the thermal stability of the T *** thermostable T phase effectively strengthens the matrix by in-hibiting dislocation ***,a highly interconnected 3D intermetallic network along the grain boundaries can still remain after long-term re-aging at 300℃,which is conducive to imposing a drag on the grain boundaries at high *** finding offers a viable route for enhancing the elevated-temperature strength of heat-resistant aluminum alloys,which could provide expanded opportunities for higher-temperature applications.

关键词： Aluminum alloys Precipitation Thermally reinforcement phase Re-aging treatment High-temperature strength

Wavelet-Based Boundary Element Method for Calculating the Band Structures of Two-Dimensional Phononic Crystals

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Acta Mechanica Solida Sinica 2021年第5期34卷 687-705页

作者： qi Wei Xingfu Ma Jiawei xiang College of Mechanical and Electrical Engineering Wenzhou UniversityWenzhou325035China

A wavelet-based boundary element method is employed to calculate the band structures of two-dimensional phononic crystals,which are composed of square or triangular lattices with scatterers of arbitrary cross *** the aid of structural periodicity,the boundary integral equations of both the scatterer and the matrix are discretized in a unit *** make the curve boundary compatible,the second-order scaling functions of the B-spline wavelet on the interval are used to approximate the geometric boundaries,while the boundary variables are interpolated by scaling functions of arbitrary *** any given angular frequency,an effective technique is given to yield matrix values related to the boundary ***,combining the periodic boundary conditions and interface conditions,linear eigenvalue equations related to the Bloch wave vector are *** numerical examples illustrate the superior performance of the proposed method by comparing with the conventional BEM.

关键词： Wavelet-based boundary element method B-spline wavelet on the interval Phononic crystal Band structure

Influence of tectonic preservation conditions on the nanopore structure of shale reservoir:A case study of Wufeng-Longmaxi Formation shale in western Hubei area,south China

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Petroleum Science 2024年第4期21卷 2203-2217页

作者： Meng xiang Shang Xu Ya-Ru Wen qi-Yang Gou Bing-Chang liu National Key Laboratory of Deep Oil and Gas China University of Petroleum(East China)Qingdao266580ShandongChina Hubei Key Laboratory of Resources and Eco-environment Geology Hubei Geological SurveyWuhan430034HubeiChina Key Laboratory of Tectonics and Petroleum Resources Ministry of EducationChina University of GeosciencesWuhan430074HubeiChina

Tectonism is one of the dominant factors affecting the shale pore ***,the control of shale pore structure by tectonic movements is still controversial,which limits the research progress of shale gas accumulation mechanism in the complex tectonic region of southern *** this study,34 samples were collected from two exploratory wells located in different tectonic *** experiments,e.g.,organic geochemistry,XRD analysis,FE-SEM,low-pressure gas adsorption,and high-pressure mercury intrusion,were conducted to fully characterize the shale *** TOC,Ro,and mineral composition of the shale samples between the two wells are similar,which reflects that the shale samples of the two wells have proximate pores-generating capacity and pores-supporting ***,the pore characteristics of shale samples from two wells are significantly *** with the stabilized zone shale,the porosity,pore volume,and specific surface area of the deformed zone shale were reduced by 60.61%,64.85%,and 27.81%,***,the macroscopic and fine pores were reduced by 54.01%and 84.95%,*** activity and uplift denudation are not conducive to pore preservation,and the rigid basement of Huangling uplift can promote pore *** three factors are important reasons for controlling the difference in pore structure between two wells *** established a conceptual model of shale pores evolution under different tectonic preservation *** study is significant to clarify the scale of shale gas formation and enrichment in complex tectonic regions,and helps in the selection of shale sweet spots.

关键词： Shale gas Pore structure Tectonic preservation conditions Shale gas enrichment mechanism

Enzyme Activity in Water-Stable Soil Aggregates as Affected by Long-Term Application of Organic Manure and Chemical Fertiliser

在线全文

学校读者我要写书评

暂无评论

Pedosphere 2013年第1期23卷 111-119页

作者： liu Yi-Ren LI xiang SHEN qi-Rong XU Yang-Chun Jiangsu Provincial Key Laboratory for Solid Organic Waste Utilization College of Resources and Environmental SciencesNanjing Agricultural University Institute of Soil Fertilizer and Resource Environment Jiangxi Academy of Agricultural Sciences

The activities of invertase, protease, urease, acid phosphomonoesterase, dehydrogenase, and catalase in different fractions of waterstable aggregates (WSA) were examined in long-term (26 years) fertilised soils. The long-term application of organic manure (OM) with chemical fertiliser (CF) significantly increased macroaggregate and decreased microaggregate percentages, enhanced the mean weight diameter, and significantly increased soil total carbon (TC) and total nitrogen (TN) contents of WSA in different size fractions. Combined fertilisation with OM and CF also increased invertase, protease, urease, acid phosphomonoesterase, dehydrogenase, and catalase activities of WSA in different size fractions. Enzyme activities were higher in macroaggregates than in microaggregates. The distribution of enzyme activities generally followed the distribution of TC and TN in WSA. The geometric mean of the enzyme activities in different WSA of OM-treated soils was significantly higher than that in soils treated with 100% CF or no fertiliser. The results indicated that the long-term combined application of OM with CF increased the aggregate stability and enzyme activity of different WSA sizes, and consequently, improved soil physical structure and increased soil microbial activity.

关键词： distribution mean weight diameter stability total carbon total nitrogen

Origin and depositional model of deep-water lacustrine sandstone deposits in the 7th and 6th members of the Yanchang Formation(Late Triassic),Binchang area,Ordos Basin,China

在线全文

学校读者我要写书评

暂无评论

Petroleum Science 2017年第1期14卷 24-36页

作者： Xi-xiang liu Xiao-qi Ding Shao-Nan Zhang Hao He State Key Laboratory of Oil and Gas Reservoir Geology and Exploitation Southwest Petroleum UniversityChengdu 610500SichuanChina School of Geoscience and Technology Southwest Petroleum UniversityChengdu 610500SichuanChina College of Energy Chengdu University of TechnologyChengdu 610059SichuanChina Second Production Plant Petro China Changqing Oilfield CompanyQinyang 745000GansuChina

Sandstones attributed to different lacustrine sediment gravity flows are present in the 7th and 6th members of the Yanchang Formation in the Ordos Basin, China. These differences in their origins led to different sandstone distributions which control the scale and connectivity of oil and gas reservoirs. Numerous cores and outcrops were analysed to understand the origins of these sandstones. The main origin of these sandstones was analysed by statistical methods, and well logging data were used to study their vertical and horizontal distributions. Results show that the sandstones in the study area accu- mulated via sandy debris flows, turbidity currents and slumping, and sandy debris flows predominate. The sand- stone associated with a single event is characteristically small in scale and exhibits poor lateral continuity. How- ever, as a result of multiple events that stacked gravity flow-related sandstones atop one another, sandstones are extensive overall, as illustrated in the cross section and isopach maps. Finally, a depositional model was developed in which sandy debris flows predominated and various other types of small-scale gravity flows occurred frequently, resulting in extensive deposition of sand bodies across a large area.

关键词： Sediment gravity flows - Sandy debris flows -Binchang area Yanchang Formation Ordos Basin

Effects of size reduction on deformation,microstructure,and surface roughness of micro components for micro metal injection molding

在线全文

学校读者我要写书评

暂无评论

International Journal of Minerals,Metallurgy and Materials 2017年第9期24卷 1021-1026页

作者： Lin liu Xin-da Wang xiang Li Xiao-tong qi Xuan-hui Qu School of Mechanical and Electrical Engineering China University of Mining and Technology(Beijing) School of Material Science and Engineering University of Science and Technology Beijing

The fabrication of 17-4PH micro spool mandrils by micro metal injection molding was described here. The effects of size reduction on deformation, microstructure and surface roughness were studied by comparing a I center dot 500 mu m micro post and a I center dot 1.7 mm cylinder after debinding and sintering. Experimental results show that slumping of the micro posts occurred due to a dramatic increase in outlet vapor pressure initiated at the thermal degradation onset temperature and the moment of gravity. Asymmetrical stress distribution within the micro component formed during the cooling stage may cause warping. Prior solvent debinding and adjustment in a thermal debinding scheme were useful for preventing the deformation of the micro components. Smaller grain size and higher micro hardness due to impeded grain growth were observed for the micro posts compared with the I center dot 1.7 mm cylinder. Surface roughness increased with distance from the gate of the micro spool mandril due to melt front advancement during mold filling and the ensuing pressure distribution. At each position, surface roughness was dictated by injection molding and increased slightly after sintering.

关键词： powder metallurgy deformation microstructure surface roughness sintering

Sangguayin preparation prevents palmitate-induced apoptosis by suppressing endoplasmic reticulum stress and autophagy in db/db mice and MIN6 pancreatic β-cells

在线全文

学校读者我要写书评

暂无评论

Chinese Journal of Natural Medicines 2020年第6期18卷 472-480页

作者： XING qi-Chang liu xiang LI Wei CHEN Yun-Zhong CHEN Jia Department of pharmacy Xiangtan Central HospitalXiangtan 411100China College of pharmacy Hubei University of Chinese MedicineWuhan 430065China

Sangguayin preparation(SGY-P) is refined from the traditional Chinese medicinal compound Sangguayin, which"clears heat and promotes fluid" and "tonifies kidney and spleen" for "Xiaoke", commonly known as ‘Diabetes mellitus’ in *** studies have shown that SGY-P could reduce insulin resistance and repair damaged pancreas in db/db mice, but the underlying mechanisms were unclear. Here, we investigated whether treatment with SGY-P could protect pancreatic β-cells from apoptosis and uncovered the underlying mechanisms. db/db mice were used to observe the hypoglycemic and islet protective effect in vivo. Apoptosis was induced in mouse insulinoma 6(MIN6) cells by palmitate, following which the cells were treated with SGY-P for elucidating the anti-apoptotic mechanism in vitro. Cell viability and nuclear morphology were detected by CCK-8 assay and Hoechst 33258 staining. The expression levels of apoptosis-, endoplasmic reticulum(ER) stress-, and autophagy-related proteins were measured by western blot. The results showed that SGY-P reduced fasting blood glucose, pancreatic pathological changes, and islet β-cell apoptosis in db/db mice. Palmitate-induced apoptosis in MIN6 cells was decreased by SGY-P treatment. Hence, SGY-P therapy exhibited a protective effect on pancreatic β-cells by decreasing the expression of cleaved caspase-3, cleaved PARP and Bax, and increasing Bcl-2 by suppressing ER stress(Bip/XBP1/IRE1α/CHOP/Caspase-12) and autophagy(LC3/p62/Atg5) pathways.2/Atg5 pathways.

关键词： Sangguayin preparation MIN6 cells Apoptosis Endoplasmic reticulum(ER)stress Autophagy Lipotoxicity