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Magnamosis for rectal reconstruction in canines

World Journal of Gastrointestinal Surgery 2025年第2期17卷 227-236页

作者： Shi-Qi Liu Hong-Ke Zhang Yi Lv xiang-hua xu Yu-Feng Li Dong-Wen Quan Medical College Xijing UniversityXi’an 710123Shaanxi ProvinceChina Department of Pediatric Surgery The First Affiliated Hospital of Xi'an Jiaotong UniversityXi'an 710061Shaanxi ProvinceChina National Local Joint Engineering Research Center for Precision Surgery&Regenerative Medicine Xi’an 710061Shaanxi ProvinceChina Department of Hepatobiliary Surgery The First Affiliated Hospital of Xi'an Jiaotong UniversityXi'an 710061Shaanxi ProvinceChina Department of Pediatric Surgery Guilin Maternal and Child Health HospitalGuilin 541001Guangxi Zhuang Autonomous RegionChina The Second Clinical Medical School Shaanxi University of Chinese MedicineXianyang 712000Shaanxi ProvinceChina

BACKGROUND The magnamosis,a minimal invasive,suture-free procedure,has been used for digestive tract or vessel reconstruction,such as gastrointestinal anastomosis,bilioenteric anastomosis,and coronary artery *** some case reports have demonstrated the potential of magnamosis for the treatment of congenital rectal atresia(RA),they cannot provide strong evidence for its widespread *** To assess the feasibility and safety of magnamosis in treating RA in dogs as compared to suturing *** findings of this study can be beneficial in guiding the clinical application of *** Thirty-six dogs were randomly assigned to the magnamosis group(n=18)and the suturing anastomosis group(n=18).The rectum was freed laparoscopically in all *** the magnamosis group,rectal anastomosis was performed using a pair of magnetic rings,while the suturing anastomosis group underwent a straight-sighted end-to-end rectal anastomosis with 4-0 absorbable *** anastomosis time was recorded,and abdominal plain film examination was performed to locate the magnets until they were expelled *** of the anastomosis were evaluated at one month,three months,and six months after *** The mean time for rectal anastomosis was significantly shorter in the magnamosis group(12.22±2.78 minutes)than the suturing anastomosis group(18.11±1.68 minutes).There was one incidence of anastomotic bleeding in the suturing anastomosis group,whereas no complication was recorded in the magnamosis *** magnets were discharged post-surgery in 7.17±1.30 days in all the *** histopathological examination revealed a smoother healing of anastomotic mucosa in the magnamosis group as compared to that in the suturing anastomosis ***,the fiber alignment was also more natural in the magnamosis group with minimal *** Rectal reconstruction using magnamosis is a feasible,safe,and effective alternative to suturing a

关键词： Rectal reconstruction Canines Magnamosis Dogs

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Study on gene expression patterns and functional pathways of peripheral blood monocytes reveals potential molecular mechanism of surgical treatment for periodontitis

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World Journal of Clinical Cases 2019年第12期7卷 1383-1392页

作者： Jin-Ji Ma Hong-Mei Liu xiang-hua xu Li-Xin Guo Qing Lin Jinan Stomatological Hospital Gaoxin Branch Jinan 250001 Shandong Province China Department of Endodontics Jinan Stomatological Hospital Jinan 250001 Shandong Province China Department of Stomatology Shandong Provincial Hospital Jinan 250001 Shandong Province China

BACKGROUND Periodontitis is a chronic inflammation of periodontal supporting tissue caused by local factors. Periodontal surgery can change the gene expression of peripheral blood mononuclear cells. However, little is known about the potential mechanism of surgical treatment for periodontitis. AIM To explore the potential molecular mechanism of surgical treatment for periodontitis. METHODS First, based on the expression profiles of genes related to surgical treatment for periodontitis, a set of expression disorder modules related to surgical treatment for periodontitis were obtained by enrichment analysis. Subsequently, based on crosstalk analysis, we proved that there was a significant crosstalk relationship between module 3 and module 5. Finally, based on predictive analysis of multidimensional regulators, we identified a series of regulatory factors, such as endogenous genes, non-coding RNAs (ncRNAs), and transcription factors, which have potential regulatory effects on periodontitis. RESULTS A total of 337 genes related to surgical treatment for periodontitis were obtained, and 3896 genes related to periodontitis were amplified. Eight expression modules of periodontitis were obtained, involving the aggregation of 2672 gene modules. These modules are mainly involved in G-protein coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger, and adenylate cyclasemodulating G-protein coupled receptor signaling pathway. In addition, eight endogenous genes (including EGF, RPS27A, and GNB3) were screened by network connectivity analysis. Finally, based on this set of potential dysfunction modules, 94 transcription factors (including NFKB1, SP1, and STAT3) and 1198 ncRNAs (including MALAT1, CRNDE, and ANCR) were revealed. These core regulators are thought to be involved in the potential molecular mechanism of periodontitis after surgical treatment. CONCLUSION Based on the results of this study, we can show biologists and pharmacists a new idea to re

关键词： Peripheral blood mononuclear cells Gene expression Dysregulation module Potential molecular mechanism Gene expression pattern

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Esophageal magnetic compression anastomosis in dogs

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World Journal of Gastroenterology 2022年第36期28卷 5313-5323页

作者： xiang-hua xu Yi Lv Shi-Qi Liu Xiao-Hai Cui Rui-Yang Suo Department of Hepatobiliary Surgery The First Affiliated Hospital of Xi’an Jiaotong UniversityXi’an 710061Shaanxi ProvinceChina National Local Joint Engineering Research Center for Precision Surgery and Regenerative Medicine The First Affiliated Hospital of Xi’an Jiaotong UniversityXi’an 710061Shaanxi ProvinceChina Department of Pediatric Surgery Xi’an Children’s HospitalXi’an 710003Shaanxi ProvinceChina Department of Thoracic Surgery The First Affiliated Hospital of Xi’an Jiaotong UniversityXi’an 710061Shaanxi ProvinceChina Zonglian College Xi’an Jiaotong University Health Science CenterXi’an 710061Shaanxi ProvinceChina

BACKGROUND Magnetic compression anastomosis(MCA)is a novel suture-free reconstruction of the digestive *** has been used in gastrointestinal anastomosis,jejunal anastomosis,cholangioenteric anastomosis and so *** traditional operative outcomes of congenital esophageal atresia and benign esophageal stricture are poor,and there are too many complications *** To test MCA technology to reconstruct the esophagus in dogs,prior to studying the feasibility and safety of MCA in *** Thirty-six dogs were randomized into either the study or control group(n=18 per group).The dogs in the study group were subjected to end-to-end esophageal anastomosis with the magnetic compression device,while those in the control group underwent hand-sewn anastomosis with 4-0 absorbable multifilament *** used interrupted single-layer inverting *** anastomosis time,gross appearance,weight and pathology of the anastomosis were evaluated at one month,three months and six months *** The anastomosis time of the MCA group was shorter than that of the hand-sewn group(7.5±1.0 min vs 12.5±1.8 min,P<0.01).In the MCA group,X-ray examination was performed every day to locate the magnetic device in the esophagus before the magnetic device fell off from the *** the hand-sewn group,dogs did not undergo X-ray *** month after the surgeries,the mean weight of the dogs in the hand-sewn group had decreased more than that of the dogs in the MCA group(11.63±0.71 kg vs 12.73±0.80 kg,P<0.05).At 3 mo and 6 mo after the operation,the dogs’weights were similar between the two groups(13.75±0.84 kg vs 14.03±0.82 kg,14.93±0.80 kg vs 15.44±0.47 kg).The number of inflammatory cells in MCA group was lower than that in hand-sewn group on 1 mo after *** MCA is an effective and safe method for esophageal *** anastomosis time of the MCA group was less than that of the hand-sewn *** study shows that MCA tec

关键词： Magnetic Anastomosis Esophagus Reconstruction Hand sewn Dog

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Giant bile duct dilatation in newborn:A case report

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World Journal of Clinical Cases 2024年第6期12卷 1150-1156页

作者： Dong-Wen Quan Peng-Gang Li xiang-hua xu Shi-Qi Liu The Second Clinical Medical School Shaanxi University of Chinese MedicineXianyang 712000Shaanxi ProvinceChina The First School of Clinical Medicine Shaanxi University of Chinese MedicineXianyang 712000Shaanxi ProvinceChina Department of Hepatobiliary Surgery The First Affiliated Hospital of Xi’an Jiaotong UniversityXi’an 710061Shaanxi ProvinceChina Department of Neonatal Surgery Xi’an Children’s Hospital Affiliated Xi’an Jiaotong UniversityXi’an 710003Shaanxi ProvinceChina

BACKGROUND Giant congenital biliary dilation(CBD)is a rare condition observed in clinical *** born with this condition often experience a poor overall health status,and the disease progresses rapidly,leading to severe biliary obstruction,infections,pressure exerted by the enlarged CBD on abdominal organs,disturbances in the internal environment,and multiple organ *** treatment of giant CBD using laparoscopy is challenging due to the high degree of variation in the shape of the bile duct and other organs,making it difficult to separate the bile duct wall from adjacent tissues or to control *** SUMMARY Herein,we present the details of an 11-d-old male newborn who was diagnosed with giant *** patient was admitted to the neonatal surgery department of our hospital due to a history of common bile duct cyst that was detected more than 3 mo ago,and also because the patient had been experiencing yellowish skin for the past 9 *** abnormal echo in the fetal abdomen was first noticed by the patient’s mother during a routine ultrasound examination at a local hospital,when the patient was at 24 wk+6 d of *** finding raised concerns about the possibility of congenital biliary dilatation(22 mm×21 mm).Subsequent ultrasound examinations at different hospitals consistently confirmed the presence of a congenital biliary *** specific treatment was administered for biliary dilatation during this period.A computed tomography scan conducted during the hospitalization revealed a large cystic mass in the right upper quadrant and pelvis,measuring approximately 9.2 cm×7.4 cm×11.3 *** on the CONCLUSION The analysis reveals that prenatal imaging techniques,such as ultrasound and magnetic resonance imaging,play a crucial role in the early diagnosis,fetal prognosis,and treatment plan for giant *** surgery for giant CBD presents certain challenges,including difficulties in separating the cyst wall,anastomosis,and hemos

关键词： Acute cholangitis Congenital biliary dilation Laparoscopic surgery vs Open hepatic duct-jejunostomy Case report

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Tumor-infiltrating platelets predict postoperative recurrence and survival in resectable pancreatic neuroendocrine tumor

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World Journal of Gastroenterology 2019年第41期25卷 6248-6257页

作者： Shuai-Shuai xu hua-xiang xu Wen-Quan Wang Shuo Li Hao Li Tian-Jiao Li Wu-Hu Zhang Liang Liu Xian-Jun Yu Department of Pancreatic Surgery Fudan University Shanghai Cancer CenterShanghai 20032China Department of Oncology Shanghai Medical CollegeFudan UniversityShanghai 200032China Shanghai Pancreatic Cancer Institute Shanghai 200032China Pancreatic Cancer Institute Fudan UniversityShanghai 200032China

BACKGROUND Platelets have been reported to participate in tumor cell growth,extravasation,epithelial–mesenchymal transition,metastasis,and drug ***,the importance of platelets in pancreatic neuroendocrine tumor(pNET)lacks adequate literature *** predictive value of tumor-infiltrating platelets(TIPs)in pNET remains *** To investigate the relationship between TIPs and the prognosis of patients with pNET following radical *** In total,113 patients who had undergone radical surgical resection with a pathologic diagnosis of pNET were enrolled in this *** analysis of cluster of differentiation 42b(CD42b)expression in the tumor specimens was performed to determine the presence of *** and multivariate analyses were used to analyze the prognostic value of *** TIPs were observed in intratumoral areas in 54 *** basic characteristics nor preoperative platelet-associated indicators showed a significant relationship with the presence of TIPs(all P>0.05).Patients with positive intratumoral CD42b expression had worse overall survival(P=0.005)and recurrence-free survival(P0.05).CONCLUSION TIPs are a useful prognostic biomarker for patients with resectable pNET,and their detection represents a promising tool for pNET treatment strategy decisions.

关键词： Tumor-infiltrating platelets Pancreatic neuroendocrine tumor Survival Recurrence Platelet count Mean platelet volume Platelet-to-lymphocyte ratio

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The clinicopathological and prognostic significance of PD-L1 expression in pancreatic cancer:A meta-analysis

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Hepatobiliary & Pancreatic Diseases International 2018年第2期17卷 95-100页

作者： He-Li Gaoa Liang Liua Zi-Hao Qi hua-xiang xu Wen-Quan Wang Chun-Tao Wu Shi-Rong Zhang Jin-Zhi xu Quan-Xing Ni Xian-Jun Yua Department of Pancreatic Surgery Fudan University Shanghai Cancer CenterShanghai 200032China Department of Oncology Shanghai Medical CollegeFudan UniversityShanghai 200032China Shanghai Pancreatic Cancer Institute Shanghai 200032China Pancreatic Cancer Institute Fudan UniversityShanghai 200032China

Background: Immunotherapy has shown promise against solid tumors. However, the clinical significance of programmed cell death 1(PD-1) and programmed cell death ligand 1(PD-L1) in pancreatic ductal adenocarcinoma(PDAC) remains unclear. This meta-analysis aimed to analyze the prognostic effect of PD-L1 in *** sources: Electronic search of the Pub Med, Cochrane Library and Web of Science was performed until December 2016. Through database searches, we identified articles describing the relationship between PD-L1 status and PDAC patient prognosis. Meta-analysis was performed to investigate the relationship between PD-1 and overall survival(OS).Results: Nine studies with 989 PDAC patients were included for PD-L1 expression analysis. And 5 studies with 688 PDAC patients were included in the prognostic analysis. The PD-L1 positive rate measured by immunohistochemistry(IHC) was higher than that measured by polymerase chain reaction(PCR)(P < 0.001). PDAC patients with high expression levels of PD-L1 had significantly reduced OS(HR = 2.34;95% CI: 1.78–3.08). Subgroup analysis showed that the prognostic effect of PD-L1 levels was similar between the IHC and PCR methods. The PD-L1 positive rate was associated with PDAC T stages; the PD-L1 positive rate in the T3–4 group was higher than that in the T1-2 group(OR = 0.37; P = 0.001).Conclusions: High PD-L1 expression levels predicted a poor prognosis in PDAC patients. Thus, PD-L1 status helps determine treatment in PDAC patients.

关键词： Pancreatic ductal adenocarcinoma Programmed cell death ligand 1 Prognosis Meta-analysis

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Active surveillance in metastatic pancreatic neuroendocrine tumors:A 20-year single-institutional experience

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World Journal of Clinical Cases 2020年第17期8卷 3751-3762页

作者： He-Li Gao Wen-Quan Wang hua-xiang xu Chun-Tao Wu Hao Li Quan-Xing Ni Xian-Jun Yu Liang Liu Department of Pancreatic Surgery Fudan University Shanghai Cancer CenterShanghai 200032China Department of Oncology Shanghai Medical CollegeFudan UniversityShanghai 200032China Shanghai Pancreatic Cancer Institute Shanghai 200032China Pancreatic Cancer Institute Fudan UniversityShanghai 200032China

BACKGROUND Pancreatic neuroendocrine tumors(PanNETs)are heterogeneous and indolent;systemic therapy is not essential for every patient with metastatic *** National Comprehensive Cancer Network guidelines state that delaying treatment is an option for PanNET with distant metastasis,if the patient has stable ***,specific factors that influence surveillance were not *** addition,data regarding the period of active surveillance in patients with metastatic PanNET are *** To specifically determine factors influencing active surveillance in patients with liver metastatic nonfunctioning PanNETs(NF-PanNETs).METHODS Seventy-six patients with liver metastatic NF-PanNETs who received active surveillance from a high-volume institution were *** to disease progression(TTP)and time to initiation of systemic therapy were *** Thirty-one(40.8%)patients had recurrent liver disease after R0 resection;45(59.2%)were diagnosed with liver *** median follow-up period was 42 mo and 90.7%patients were observed to have disease *** median TTP(mTTP)was 10 *** analysis showed that the largest axis of the liver metastasis>5 mm(P=0.04),non-resection of the primary tumor(P=0.024),and T3-4 stage(P=0.028)were associated with a shorter *** mTTP in patients with no risk factors was 24 mo,which was significantly longer than that in patients with one(10 mo)or more(6 mo)risk factors(P<0.001).A nomogram with three risk factors showed reasonable calibration,with a C-index of 0.603(95%confidence interval:0.47-0.74).CONCLUSION Active surveillance may only be safe for metastatic NF-PanNET patients with favorable risk factors,and other patients progressed rapidly without *** studies with a larger sample size and a control group are needed.

关键词： Pancreatic neuroendocrine tumor Liver metastasis Active surveillance Prognosis Nomogram

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Haemoglobin,albumin,lymphocyte and platelet predicts postoperative survival in pancreatic cancer

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World Journal of Gastroenterology 2020年第8期26卷 828-838页

作者： Shuai-Shuai xu Shuo Li hua-xiang xu Hao Li Chun-Tao Wu Wen-Quan Wang He-Li Gao Wang Jiang Wu-Hu Zhang Tian-Jiao Li Quan-Xing Ni Liang Liu Xian-Jun Yu Department of Pancreatic Surgery Fudan UniversityShanghai Cancer CenterShanghai 20032China Pancreatic Cancer Institute Fudan UniversityShanghai 200032China Department of Oncology Shanghai Medical CollegeFudan UniversityShanghai 200032China

BACKGROUND Systemic inflammation and nutrition status play an important role in cancer *** combined index of hemoglobin,albumin,lymphocyte,and platelet(HALP),consisting of haemoglobin,albumin,lymphocytes,and platelets,is considered as a novel marker to reflect both systemic inflammation and nutrition ***,no studies have investigated the relationship between HALP and survival of patients with pancreatic cancer following radical *** To evaluate the prognostic value of preoperative HALP in pancreatic cancer *** The preoperative serum levels of hemoglobin,albumin,lymphocyte counts,and platelet counts were routinely detected in 582 pancreatic adenocarcinoma patients who underwent radical *** relationship between postoperative survival and the preoperative level of HALP was *** Low levels of HALP were significantly associated with lymph node metastasis(P=0.002),poor tumor differentiation(P=0.032),high TNM stage(P=0.008),female patients(P=0.005)and tumor location in the head of the pancreas(P<0.001).Low levels of HALP were associated with early recurrence[7.3 mo vs 16.3 mo,P<0.001 for recurrence-free survival(RFS)]and short survival[11.5 mo vs 23.6 mo,P<0.001 for overall survival(OS)]in patients with resected pancreatic adenocarcinoma.A low level of HALP was an independent risk factor for early recurrence and short survival irrespective of sex and tumor *** Low levels of HALP may be a significant risk factor for RFS and OS in patients with resected pancreatic cancer.

关键词： Pancreatic adenocarcinoma HALP Systemic inflammation Nutrition status Postoperative survival

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Resting-state brain network remodeling after different nerve reconstruction surgeries:a functional magnetic resonance imaging study in brachial plexus injury rats

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Neural Regeneration Research 2025年第5期20卷 1495-1504页

作者： Yunting xiang xiangxin Xing xuyun hua Yuwen Zhang Xin xue Jiajia Wu Mouxiong Zheng He Wang Jianguang xu School of Rehabilitation Science Shanghai University of Traditional Chinese MedicineShanghaiChina Engineering Research Center of Traditional Chinese Medicine Intelligent Rehabilitation Ministry of EducationShanghaiChina Department of Rehabilitation Medicine Yueyang Hospital of Integrated Traditional Chinese and Western MedicineShanghai University of Traditional Chinese MedicineShanghaiChina Department of Traumatology and Orthopedics Yueyang Hospital of Integrated Traditional Chinese and Western MedicineShanghai University of Traditional Chinese MedicineShanghaiChina Institute of Science and Technology for Brain-Inspired Intelligence Fudan UniversityShanghaiChina Key Laboratory of Computational Neuroscience and Brain-Inspired Intelligence(Fudan University) Ministry of EducationShanghaiChina Human Phenome Institute Fudan UniversityShanghaiChina

Distinct brain remodeling has been found after different nerve reconstruction strategies,including motor representation of the affected ***,differences among reconstruction strategies at the brain network level have not been *** study aimed to explore intranetwork changes related to altered peripheral neural pathways after different nerve reconstruction surgeries,including nerve repair,endto-end nerve transfer,and end-to-side nerve ***–Dawley rats underwent complete left brachial plexus transection and were divided into four equal groups of eight:no nerve repair,grafted nerve repair,phrenic nerve end-to-end transfer,and end-to-side transfer with a graft sutured to the anterior upper ***-state brain functional magnetic resonance imaging was obtained 7 months after *** independent component analysis algorithm was utilized to identify group-level network components of interest and extract resting-state functional connectivity values of each voxel within the *** in intra-network resting-state functional connectivity were compared among the *** muscle reinnervation was assessed by behavioral observation(elbow flexion)and *** results showed that alterations in the sensorimotor and interoception networks were mostly related to changes in the peripheral neural *** repair was related to enhanced connectivity within the sensorimotor network,while end-to-side nerve transfer might be more beneficial for restoring control over the affected limb by the original motor *** thalamic-cortical pathway was enhanced within the interoception network after nerve repair and end-to-end nerve *** areas related to cognition and emotion were enhanced after end-to-side nerve *** study revealed important brain networks related to different nerve *** networks may be potential targets for enhancing motor recovery.

关键词： brain functional networks end-to-end nerve transfer end-to-side nerve transfer independent component analysis nerve repair peripheral plexus injury resting-state functional connectivity

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Rapid and scalable preparation of highly quaternized polymer networks to achieve efficient simultaneous adsorption of iodide and pertechnetate

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Science China Chemistry 2025年第1期68卷 337-349页

作者： Meiyun xu Song Gu Fulong Chen Zhe Zhao Peng Liu Daoben hua State Key Laboratory of Radiation Medicine and Protection School of Radiological and Interdisciplinary Sciences(RAD-X)Collaborative Innovation Center of Radiological Medicine of Jiangsu Higher Education InstitutionsSoochow UniversitySuzhou 215123China

The efficient separation of fission products,such as TcO_(4)^(-)and I^(-),holds strategic significance for the management of radioactive wastes and environmental *** this study,we propose an ultrafast strategy for scalable preparation of a highly quaternized organic network to facilitate efficient and synchronous separation of iodide and *** network can be prepared within a few minutes at room temperature,using polyethylenimine and 1,2,4,5-tetrakis(bromomethyl)benzene as building *** chlorine replacement,the network,abundantly decorated with quaternary ammonium groups(Cl@QPN),exhibits an ultrahigh positive charge density of 7.6 mmol/*** enables the rapid and efficient enrichment of target anions through strong electrostatic Coulomb *** a result,Cl@QPN exhibits significantly higher adsorption rate constants of 0.830 g/(mg min)for ReO_(4)^(-)(a nonradioactive surrogate of TcO_(4)^(-))and 0.677 g/(mg min)for I-compared to other ***,it possesses high adsorption capacities,reaching 1,681 mg/g for ReO_(4)^(-)and 917.4 mg/g for I-.Cl@QPN also demonstrates good selectivity towards target ions and shows efficient adsorption for^(99)TcO_(4)^(-).Additionally,Cl@QPN exhibits high dynamic processing capacities,handling up to 3,100 and 7,400 kg of simulated streams per kilogram of material for I-and ReO_(4)^(-),respectively.

关键词： quaternized polymer networks adsorption pertechnetate iodide liquid radioactive streams

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