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Relationship between clinical features and intestinal microbiota in Chinese patients with ulcerative colitis

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World Journal of Gastroenterology 2021年第28期27卷 4722-4737页

作者： Xu-Xia He Ying-He Li Peng-Guang Yan xiang-chen meng Chu-Yan chen Ke-Min Li Jing-Nan Li Department of Gastroenterology Peking Union Medical College HospitalPeking Union Medical College and Chinese Academy of Medical ScienceBeijing 100730China

BACKGROUND Dysbacteriosis may be a crucial environmental factor for ulcerative colitis(UC).Further study is required on microbiota alterations in the gastrointestinal tract of patients with UC for better clinical management and *** To analyze the relationship between different clinical features and the intestinal microbiota,including bacteria and fungi,in Chinese patients with *** Eligible inpatients were enrolled from January 1,2018 to June 30,2019,and stool and mucosa samples were *** was diagnosed by endoscopy,pathology,Mayo Score,and Montreal *** amplicon sequencing of 16S rRNA gene and fungal internal transcribed spacer gene was used to detect the intestinal microbiota *** diversity,principal component analysis,similarity analysis,and Metastats analysis were employed to evaluate differences among *** A total of 89 patients with UC and 33 non-inflammatory bowel disease(IBD)controls were *** bacterial analysis,72 stool and 48 mucosa samples were obtained from patients with UC and 21 stool and 12 mucosa samples were obtained from the *** fungal analysis,stool samples were obtained from 43 patients with UC and 15 controls.A significant difference existed between the fecal and mucosal bacteria of patients with ***α-diversity of intestinal bacteria and the relative abundance of some families,such as Lachnospiraceae and Ruminococcaceae,decreased with the increasing severity of bowel inflammation,while Escherichia-Shigella showed the opposite *** intermicrobial correlations in UC in remission than in active patients were *** bacteriafungi correlations became single and uneven in patients with *** The intestinal bacteria flora of patients with UC differs significantly in terms of various sample types and disease *** intermicrobial correlations change in patients with UC compared with non-IBD controls.

关键词： Ulcerative colitis Intestinal microbiota Intermicrobial correlation Bacteria Fungi Chinese

Detection of blood AFPmRNA in nude mice bearing human HCC using nested RT-PCR and its significance

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World Journal of Gastroenterology 1998年第3期4卷 88-90页

作者： LIU Yang, ZHANG BaiHe, QIAN Guangxiang, chen Han and WU mengChao

ＤｅｔｅｃｔｉｏｎｏｆｂｌｏｏｄＡＦＰｍＲＮＡｉｎｎｕｄｅｍｉｃｅｂｅａｒｉｎｇｈｕｍａｎＨＣＣｕｓｉｎｇｎｅｓｔｅｄＲＴＰＣＲａｎｄｉｔｓｓｉｇｎｉｆｉｃａｎｃｅＬＩＵＹａｎｇ，ＺＨＡＮＧＢａｉＨｅ，ＱＩＡＮＧ... 详细信息

ＤｅｔｅｃｔｉｏｎｏｆｂｌｏｏｄＡＦＰｍＲＮＡｉｎｎｕｄｅｍｉｃｅｂｅａｒｉｎｇｈｕｍａｎＨＣＣｕｓｉｎｇｎｅｓｔｅｄＲＴＰＣＲａｎｄｉｔｓｓｉｇｎｉｆｉｃａｎｃｅＬＩＵＹａｎｇ，ＺＨＡＮＧＢａｉＨｅ，ＱＩＡＮＧｕａｎｇＸｉａｎｇ，ＣＨＥＮＨａ...

关键词： carcinoma, hepatocellular/pathology lung neoplasms/secondary kidney neoplasms/secondary alphafetoproteins/analysis RNA, messenger polymerase chain reaction

Coupling Co_(2)P nanoparticles onto N,P-doped biomass-derived carbon as efficient electrocatalysts for flexible Zn-air batteries

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Rare Metals 2024年第10期43卷 4982-4991页

作者： Shu-Qi Li Kang Sun Yan-Yan Liu Shu-Ling Liu Jing-Jing Zhou Wen-Bo Zhang Yi-Hang Lu xiang-meng chen Xiao-Peng Wang Bao-Jun Li Jian-Chun Jiang College of Science Henan Agricultural UniversityZhengzhou 450002China Department of Chemistry Tsinghua UniversityBeijing 100084China College of Chemistry Zhengzhou UniversityZhengzhou 450001China Institute of Chemical Industry of Forest Products National Engineering Lab for Biomass Chemical UtilizationKey and Open Lab on Forest Chemical EngineeringChinese Academy of ForestryState Forestry AdministrationNanjing 210042China

Catalysts for oxygen conversion play an important role in energy storage and conversion technologies such as metal-air *** highly active stable,and low-cost catalysts is critical for further implementation of the above ***,an efficien oxygen electrocatalyst with Co_(2)P nanoparticles(NPs)coupling onto N,P-doped porous carbon nanosheets(Co_(2)P@NPAC)derived from cotton stalk is successfully designed and prepared via activation,hydrothermal treatment,and pyrolysis *** excellent properties of electrical conductivity,mass transfer,and synergistic integration between Co_(2)P and N,P-doped carbon endow Co_(2)P@NPAC composite with superior oxygen reduction reaction/oxygen evolution reaction(ORR/OER)***_(2)P@NPAC catalyst achieves ORR activity with half-wave potential of 0.85 V and high OER activity with overpotential of 261 mV at 10 mA·cm^(-2).The flexible Zn-air batteries(ZABs)based on Co_(2)P@NPAC catalyst revea outstanding performances with a high open-circuit voltage(1.38 V),a peak power density of(75 mW·cm^(-2)),and a low charge-discharge voltage gap(0.74 V,~200 cycles)The flexible ZABs express the stable discharge-charge voltage gap at various bending *** work provides interesting inspiration for the ingenious design of efficien carbon electrocatalysts from biomass with potential application of energy storage and conversion devices.

关键词： Cotton stalk N P-doped activated carbon Oxygen reduction reaction Flexible Zn-air batteries

Optimization of the quenching method for metabolomics analysis of Lactobacillus bulgaricus

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2014年第4期15卷 333-342页

作者： Ming-ming chen Ai-li LI Mao-cheng SUN Zhen FENG xiang-chen meng Ying WANG MOE Key Laboratory of Dairy Science College of Food ScienceNortheast Agricultural University School of Public Health Jilin Medical College

This study proposed a quenching protocol for metabolite analysis of Lactobacillus delbrueckii *** cells were quenched with 60% methanol/water,80% methanol/glycerol,or 80% methanol/*** effect of the quenching process was assessed by the optical density（OD）-based method,flow cytometry,and gas chromatography-mass spectrometry（GC-MS）.The principal component analysis（PCA） and orthogonal partial least squares-discriminant analysis（OPLS-DA） were employed for metabolite *** results indicated that quenching with 80% methanol/water solution led to less damage to the *** cells,characterized by the lower relative fraction of prodium iodide（PI）-labeled cells and the higher OD recovery *** GC-MS analysis,higher levels of intracellular metabolites（including focal glutamic acid,aspartic acid,alanine,and AMP） and a lower leakage rate were detected in the sample quenched with 80% methanol/water compared with the *** conclusion,we suggested a higher concentration of cold methanol quenching for *** metabolomics due to its decreasing metabolite leakage.

关键词： Metabolomics Quenching method Lactobacillus bulgaricus Leakage

海藻酸钠-酚醛树脂复合凝胶球制备及对Au(Ⅲ)的吸附性能

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高校化学工程学报 2022年第5期36卷 684-692页

作者：刘云向勇陈香萌弓浩宇徐丽郑州大学化工学院河南郑州450001

以海藻酸钠(SA)为凝胶骨架,采用包埋共混法将酚醛树脂(RF)与SA复合,制备海藻酸钠-酚醛树脂复合凝胶球(SA-RF),用于从含金废液中回收金离子Au(Ⅲ)。间歇吸附实验表明,凝胶球在25℃时对Au(Ⅲ)的最大吸附量达508 mg×g^(-1),且可在多金属... 详细信息

以海藻酸钠(SA)为凝胶骨架,采用包埋共混法将酚醛树脂(RF)与SA复合,制备海藻酸钠-酚醛树脂复合凝胶球(SA-RF),用于从含金废液中回收金离子Au(Ⅲ)。间歇吸附实验表明,凝胶球在25℃时对Au(Ⅲ)的最大吸附量达508 mg×g^(-1),且可在多金属离子溶液中选择性地吸附Au(Ⅲ)。吸附动力学和热力学研究表明,Au(Ⅲ)在SA-RF上的吸附行为由多分子层化学吸附主导,适合用伪二级动力学模型和Freundlich等温线模型描述,吸附过程自发吸热,推测吸附机制为氧化还原、配位作用、离子交换和静电吸引的共同作用。这种合成简单、易分离、低成本的吸附剂在兼备高吸附性能的同时,能有效克服粉体材料难回收的缺点,为新型吸附剂的制备提供新思路,也使从水溶液中大规模回收金资源成为可能。

关键词：酚醛树脂海藻酸钠复合凝胶性能 Au(Ⅲ)吸附

Effects of probiotics and prebiotics on intestinal microbiota in mice with acute colitis based on 16S rRNA gene sequencing

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Chinese Medical Journal 2019年第15期132卷 1833-1842页

作者： Ya-Nan Wang xiang-chen meng Yi-Fan Dong Xin-Hua Zhao Jia-Ming Qian Hong-Ying Wang Jing-Nan Li Department of Gastroenterology Peking Union Medical College Hospital Peking Union Medical College Chinese Academy of Medical Sciences Beijing 100730 China National Cancer Center/Cancer Hospital Peking Union Medical College Chinese Academy of Medical Sciences Beijing 100021 China

Background:Imbalance of intestinal microbiota was closely related to *** these circumstances,regulation of enteric flora may be beneficial to the repair of *** aimed to investigate the effects of probiotics (Bifidobacterium and Lactobacillus),prebiotics and their combination on inflammation,and microflora in mice of acute ***:C57BL/6J mice were divided into six groups randomly (blank control group,model control group,probiotics group,synbiotics group,lactitol group and probiotics + lactitol group).Each group was given 2.5% dextran sulfate sodium drinking water for 5 days other than the blank control *** for the model control group,the other four groups were intervened with probiotics,synbiotics (probiotics and inulin),lactitol,and probiotics + *** were sacrificed after 1 week of gavage,and pathologic scores were *** feces of different periods and intestinal mucosa samples were collected to analyze the differences of intestinal microbiota by 16S rRNA *** of two groups or multiple groups were statistically examined through unpaired Student t test and analysis of variance (ANOVA),***,Tukey,Anosim,and metastats analysis were used to compare differences of microbiota among different ***:After gavage for 1 week,the pathologic scores of groups with the intervention were significantly lower than those in the model control group,and the difference was statistically significant (P < 0.05).The model control group was higher in the genus of Bacteroides (relative abundance:0.3679 vs.0.0099,P =0.0016) and lower in Lactobacillus (relative abundance:0.0020 vs.0.0122,P =0.0188),Roseburia (relative abundance:0.0004 vs.0.0109,P =0.0157),compared with the blank control ***,the same phenomenon was not found in groups gavaged with probiotics and *** with model control group,mice with intervention were increased with Bifidobacterium (relative abundance:0.017

关键词： Probiotics Lactitol Intestinal microbiota Akkermansia

Unraveling the therapeutic potential of Calculus Bovis in liver cancer:A novel step for targeted cancer treatment

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World Journal of Gastroenterology 2025年第3期31卷 127-129页

作者： Xing-Yu chen xiang Lan Department of Hepatobiliary Surgery The First Affiliated Hospital of Chongqing Medical UniversityChongqing 400016China

Hepatocellular carcinoma is one of the leading causes of cancer-related deaths globally,and effective treatments are urgently *** present study aimed to investigate the inhibitory effect of Calculus Bovis(CB)on liver cancer and the underlying *** inhibited M2 tumor-associated macrophage polarization and modulated the Wnt/β-catenin signaling pathway,thereby suppressing the proliferation of liver cancer *** inhibitory effect on liver cancer growth was confirmed by both in vivo and in vitro experiments(detailed by Huang et al).The present study provides a theoretical basis for the application of CB for the treatment of liver cancer,providing new avenues for liver cancer treatment.

关键词： Calculus Bovis Liver cancer M2 tumor-associated macrophage polarization Wnt/β-catenin Pathway Tumor microenvironment

RAPD-PCR Analysis on Genetic Relationships Between Cultivars of Tree Peony

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Agricultural Sciences in China 2002年第7期1卷 792-797页

作者： ZHENG Guo-sheng, chen xiang-ming and meng Li(College of Life Science, Shandong Agricultural University, Taian 271018 , P.R.China Hefei EducationCollege,Hefei 230001 , P.R. China) College of Life Science Shandong Agricultural University Taian 271018 P.R. China

Random amplified polymorphic DNA (RAPD) was used to analyze genetic polymophism of 35 Tree Peony cultivars with 7 different color groups. Thirty four primers amplified 418 DNA fragments and 337 polymorphic bands (80.6%), including specific DNA markers for 18 cultivars that could be used to differentiate cultivars. The UPCMA method was used to analyze the genetic relationship among cultivars. The results showed that 35 Peony cultivars could be divided into 2 cluster groups when using similarity criteria of 1.5, and into 4 cluster groups when using similarity criteria of 1.0. The result confirmed that the flower color has no relation to the genetic clusters and the Tree Peony cultivars originated from the same area has close genetic relationship. Therefore, genetic background has no large effect on the genetic relationship. The sequence based on polymorphic rate from high to low was Blue groups > Yellow groups > Bark red groups > Blake groups > White groups>Green groups>Red groups.

关键词： Cultivars of Tree Peony cultivars Different color groups Genetic polymorphism DNA-fin-gerprinting RAPD analysis

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基于生成对抗网络的肺结节良恶性诊断算法

东北大学学报（自然科学版） 2022年第1期43卷 24-32页

作者：罗家健冯宝陈相猛顾正晖华南理工大学自动化科学与工程学院广东广州510640 桂林航天工业学院医学人工智能实验室广西桂林541004 江门市中心医院放射科广东江门529030

针对实性肺结节CT影像数据量少、人工标注耗时耗力等问题,提出一种结合生成对抗网络和集成学习的实性肺结节良恶性计算机辅助诊断方法.首先,使用基于梯度惩罚的生成对抗网络对肺结节CT影像数据集进行扩充,缓解由数据量少、样本类别不均... 详细信息

针对实性肺结节CT影像数据量少、人工标注耗时耗力等问题,提出一种结合生成对抗网络和集成学习的实性肺结节良恶性计算机辅助诊断方法.首先,使用基于梯度惩罚的生成对抗网络对肺结节CT影像数据集进行扩充,缓解由数据量少、样本类别不均衡导致的模型过拟合.然后,利用卷积神经网络进行CT影像特征提取,并通过主成分分析对深度特征进行降维.最后,联合CT图像特征和有效临床信息,采用集成学习方法构建分类模型预测实性肺结节良恶性.基于多中心临床数据分析表明,相比于传统卷积神经网络模型,所提出方法有更好的预测性能.

关键词：实性肺结节肺腺癌计算机断层扫描生成对抗网络集成学习