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响应面分析法优化女贞子蛋白质提取工艺及功能性研究

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中国调味品 2024年第7期49卷 1-9页

作者：沈祥皓陈昌威付靖雯杨柳莺王则程杨金玉盘赛昆江苏海洋大学海洋食品与生物工程学院江苏连云港222005 江苏省海洋生物资源与环境重点实验室江苏连云港222000 江苏省海洋生物产业技术协同创新中心江苏连云港222000

为了进一步研究和开发女贞子在食品领域的应用,以提高女贞子蛋白质的提取率为目的进行研究。将超声辅助碱溶酸沉法提取干燥女贞子果实中的蛋白质工艺作为基础,通过单因素实验和响应面实验考察提取温度、提取时间、液料比、pH值4个因素... 详细信息

为了进一步研究和开发女贞子在食品领域的应用,以提高女贞子蛋白质的提取率为目的进行研究。将超声辅助碱溶酸沉法提取干燥女贞子果实中的蛋白质工艺作为基础,通过单因素实验和响应面实验考察提取温度、提取时间、液料比、pH值4个因素对女贞子蛋白质提取率的影响,以确定最佳的提取工艺并测定其功能性。实验结果表明,女贞子蛋白质的最佳提取条件为提取温度45℃、提取时间40 min、液料比为25∶1、pH值9.0,该条件下最高提取率为85.09%,实际提取率与模型预测值无显著性差异。此时所得的女贞子蛋白质在持水性、乳化性、乳化稳定性方面均高于市售成品大豆蛋白,持油性、起泡性、泡沫稳定性均低于大豆蛋白,且具有较强的抗氧化能力。因此,该方法提取的女贞子蛋白质具有很高的应用价值和发展潜力,可为女贞子在饮品、调味品等食品领域的高值化利用提供理论依据。

关键词：女贞子蛋白质工艺优化功能性食品

Activation of disease resistance against Botryosphaeria dothidea by downregulating the expression of MdSYP121 in apple

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Horticulture Research 2018年第1期5卷 652-667页

作者： Xiaowen He Yanhong Huo Xiuxia Liu Qianqian Zhou Shouqian Feng xiang shen Baohua Li Shujing Wu Xuesen Chen State Key Laboratory of Crop Biology College of Horticulture Sciences and EngineeringShandong Agricultural UniversityDaizong Street No.61Tai’anShandong271018China College of Plant Health and Medicine Qingdao Agricultural UniversityChangcheng Road No.700QingdaoShandong266109China

In plants,the vesicle fusion process plays a vital role in pathogen defence.However,the importance of the vesicle fusion process in apple ring rot has not been studied.Here,we isolated and characterised the apple syntaxin gene MdSYP121.Silencing the MdSYP121 gene in transgenic apple calli increased tolerance to Botryosphaeria dothidea infection;this increased tolerance was correlated with salicylic acid(SA)synthesis-related and signalling-related gene transcription.In contrast,overexpressing MdSYP121 in apple calli resulted in the opposite phenotypes.In addition,the results of RNA sequencing(RNA-Seq)and quantitative real-time PCR(qRT-PCR)assays suggested that MdSYP121 plays an important role in responses to oxidation–reduction reactions.Silencing MdSYP121 in apple calli enhanced the expression levels of reactive oxygen species(ROS)-related genes and the activity of ROS-related enzymes.The enhanced defence response status in MdSYP121-RNAi lines suggests that syntaxins are involved in the defence response to B.dothidea.More importantly,we showed that MdSYP121 forms a soluble N-ethylmaleimide-sensitive factor attachment protein receptor(SNARE)complex with MdSNAP33,and the complex may participate in regulating resistance to B.dothidea.In conclusion,by regulating the interaction of SA pathway and oxidation–reduction process,MdSYP121 can influence the pathogen infection process in apple.

关键词： infection resistance regulating

Exceptional thermal stability of ultrafine-grained long-period stacking ordered Mg alloy

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Rare Metals 2022年第5期41卷 1537-1542页

作者： Shuai-Jun Ding Li-Dong Xu Xue-Cheng Cai shen-xiang-Yu Gu Kang-Kang Wen Hui Yu Zheng Chen Bao-Ru Sun sheng-Wei Xin Tong-De shen State Key Laboratory of Metastable Materials Science and Technology Clean Nano Energy CenterYanshan UniversityQinhuangdao 066004China Department of Mechanical Engineering the University of Hong KongHong Kong 999077China School of Material Science and Engineering China University of Mining and TechnologyXuzhou 221116China School of Materials Science and Engineering Hebei University of TechnologyTianjin 300132China

The hierarchical long-period stacking ordered(LPSO)architecture composed mainly of an ultrafine-grained LPSO matrix has been demonstrated to be a feasible design strategy for achieving ultra-strong Mg alloys.Here,we r... 详细信息

关键词： grain alloy alloy

Physical Layer Encryption of OFDM-PON Based on Quantum Noise Stream Cipher with Polar Code

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China Communications 2024年第3期21卷 174-188页

作者： Xu Yinbo Gao Mingyi Zhu Huaqing Chen Bowen xiang Lian shen Gangxiang Jiangsu Engineering Research Center of Novel Optical Fiber Technology and Communication Network Suzhou Key Laboratory of Advanced Optical Communication Network Technologythe School of Electronic and Information EngineeringSoochow UniversityNo.1 Shizi StreetSuzhou 215006China

Orthogonal frequency division multiplexing passive optical network(OFDM-PON) has superior anti-dispersion property to operate in the C-band of fiber for increased optical power budget. However,the downlink broadcast exposes the physical layer vulnerable to the threat of illegal eavesdropping. Quantum noise stream cipher(QNSC) is a classic physical layer encryption method and well compatible with the OFDM-PON. Meanwhile, it is indispensable to exploit forward error correction(FEC) to control errors in data transmission. However, when QNSC and FEC are jointly coded, the redundant information becomes heavier and thus the code rate of the transmitted signal will be largely reduced. In this work, we propose a physical layer encryption scheme based on polar-code-assisted QNSC. In order to improve the code rate and security of the transmitted signal, we exploit chaotic sequences to yield the redundant bits and utilize the redundant information of the polar code to generate the higher-order encrypted signal in the QNSC scheme with the operation of the interleaver.We experimentally demonstrate the encrypted 16/64-QAM, 16/256-QAM, 16/1024-QAM, 16/4096-QAM QNSC signals transmitted over 30-km standard single mode fiber. For the transmitted 16/4096-QAM QNSC signal, compared with the conventional QNSC method, the proposed method increases the code rate from 0.1 to 0.32 with enhanced security.

关键词： physical layer encryption polar code quantum noise stream cipher

Microstructure design of advanced magnesium-air battery anodes

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Journal of Magnesium and Alloys 2024年第2期12卷 443-464页

作者： Xu Huang Qingwei Dai Qing xiang Na Yang Gaopeng Zhang Ao shen Wanming Li School of Metallurgy and Materials Engineering Chongqing University of Science and TechnologyChongqing 401331China School of Materials and Architectural Engineering Guizhou Normal UniversityGuiyang 550025China School of Material and Metallurgy University of Science and Technology LiaoningAnshan 114051China

Metal-air battery is an environmental friendly energy storage system with unique open structure.Magnesium(Mg)and its alloys have been extensively attempted as anodes for air batteries due to high theoretical energy density,low cost,and recyclability.However,the study on Mg-air battery(MAB)is still at the laboratory level currently,mainly owing to the low anodic efficiency caused by the poor corrosion resistance.In order to reduce corrosion losses and achieve optimal utilization efficiency of Mg anode,the design strategies are reviewed from microstructure perspectives.Firstly,the corrosion behaviors have been discussed,especially the negative difference effect derived by hydrogen evolution.Special attention is given to the effect of anode micro-structures on the MAB,which includes grain size,grain orientation,second phases,crystal structure,twins,and dislocations.For further improvement,the discharge performance,long period stacking ordered phase and its enhancing effect are considered.Meanwhile,given the current debates over Mg dendrites,the potential risk,the impact on discharge,and the elimination strategies are discussed.Microstructure control and single crystal would be promising ways for MAB anode.

关键词： Magnesium Air battery Anode Microstructure Anodic efficiency

Self-sufficient nanoparticles with dual-enzyme activity trigger radical storms and activate cascade-amplified antitumor immunologic responses

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Acta Pharmaceutica Sinica B 2024年第2期14卷 821-835页

作者： Liping Bai Jin Yang Siting Yu Zhongzheng xiang Yuanyuan Zeng Meiling shen Xiaorong Kou Qinjie Wu Changyang Gong Department of Biotherapy Cancer Center and State Key Laboratory of BiotherapyWest China HospitalSichuan UniversityChengdu 610041China

Radiotherapy(RT) can potentially induce systemic immune responses by initiating immunogenic cell death(ICD) of tumor cells.However,RT-induced antitumor immunologic responses are sporadic and insufficient against cancer metastases.Herein,we construct multifunctional self-sufficient nanoparticles(MARS) with dual-enzyme activity(GOx and peroxidase-like) to trigger radical storms and activate the cascade-amplified systemic immune responses to suppress both local tumors and metastatic relapse.In addition to limiting the Warburg effect to actualize starvation therapy,MARS catalyzes glucose to produce hydrogen peroxide(H_(2)O_(2)),which is then used in the Cu^(+)-mediated Fenton-like reaction and RT sensitization.RT and chemodynamic therapy produce reactive oxygen species in the form of radical storms,which have a robust ICD impact on mobilizing the immune system.Thus,when MARS is combined with RT,potent systemic antitumor immunity can be generated by activating antigen-presenting cells,promoting dendritic cells maturation,increasing the infiltration of cytotoxic T lymphocytes,and reprogramming the immuno suppre ssive tumor microenvironment.Furthermore,the synergistic therapy of RT and MARS effectively suppresses local tumor growth,increases mouse longevity,and results in a 90% reduction in lung metastasis and postoperative recurrence.Overall,we provide a viable approach to treating cancer by inducing radical storms and activating cascade-amplified systemic immunity.

关键词： Dual-enzyme activity Radiotherapy Chemodynamic therapy Fenton-likereaction Radical storms Immunogeniccell death Tumormicroenvironment Systemicimmunity

Design of High Performance MXene/Oxide Structure Memristors for Image Recognition Applications

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Chinese Journal of Electronics 2024年第2期33卷 336-345页

作者： Xiaojuan LIAN Yuelin SHI Xinyi shen xiang WAN Zhikuang CAI Lei WANG The College of Integrated Circuit Science and Engineering Nanjing University of Posts and Telecommunications The National and Local Joint Engineering Laboratory of RF Integration and Micro-Assembly Technology Nanjing University of Posts and Telecommunications

Recent popularity to realize image recognition by memristor-based neural network hardware systems has been witnessed owing to their similarities to neurons and synapses.However,the stochastic formation of conductive filaments inside the oxide memristor devices inevitably makes them face some drawbacks,represented by relatively higher power consumption and severer resistance switching variability.In this work,we design and fabricate the Ag/MXene（Ti3C2）/SiO2/Pt memristor after considering the stronger interactions between Ti3C2and Ag ions,which lead to a Ti3C2/SiO2structure memristor owning to much lower "SET" voltage and smaller resistance switching fluctuation than pure SiO2memristor.Furthermore,the conductances of the Ag/Ti3C2/SiO2/Pt memristor have been modulated by changing the number of the applied programming pulse,and two typical biological behaviors,i.e.,longterm potentiation and long-term depression,have been achieved.Finally,device conductances are introduced into an integrated device-to-algorithm framework as synaptic weights,by which the MNIST hand-written digits are recognized with accuracy up to 77.39%.

关键词： Memristor MXene/Oxide structure Electrical characteristics First-principles calculations Image recognition

Isolation and Manipulation of Quantitative Trait Loci for Disease Resistance in Rice Using a Candidate Gene Approach

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Molecular Plant 2008年第5期1卷 786-793页

作者： Ke-Ming Hu De-Yun Qiu xiang-Ling shen xiang-Hua Li Shi-Ping Wang National Key Laboratory of Crop Genetic Improvement National Center of Plant Gene Research (Wuhan) Huazhong Agricultural University Wuhan 430070 China

Bacterial blight caused by Xanthomonas oryzae pv. oryzae and fungal blast caused by Magnaporthe grisea result in heavy production losses in rice, a main staple food for approximately 50% of the world＇s population. Application of host resistance to these pathogens is the most economical and environment-friendly approach to solve this problem. Quantitative trait loci （QTLs） controlling quantitative resistance are valuable sources for broad-spectrum and durable disease resistance. Although large numbers of QTLs for bacterial blight and blast resistance have been identified, these sources have not been used effectively in rice improvement because of the complex genetic control of quantitative resistance and because the genes underlying resistance QTLs are unknown. To isolate disease resistance QTLs, we established a candidate gene strategy that integrates linkage map, expression profile, and functional complementation analyses. This strategy has proven to be applicable for identifying the genes underlying minor resistance QTLs in rice-Xoo and rice-M, grisea systems and it may also help to shed light on disease resistance QTLs of other cereals. Our results also suggest that a single minor QTL can be used in rice improvement by modulating the expression of the gene underlying the QTL. Pyramiding two or three minor QTL genes, whose expression can be managed and that function in different defense signal transduction pathways, may allow the breeding of rice cultivars that are highly resistant to bacterial blight and blast.

关键词： bacterial blight blast Oryza sativa quantitative resistance QTL.

OPTIMIZATION OF CORONARY STENT STRUCTURE DESIGN FOR MAXIMIZING THE ANTICOMPRESSION MECHANICAL PROPERTY

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Chinese Journal of Mechanical Engineering 2008年第6期21卷 98-102页

作者： shen xiang YI Hong NI Zhonghua GU Xingzhong Jiangsu Province Key Laboratory for Design and Manufacture of Micro-Nano Biomedical Instruments Southeast University Nanjing 211189 China

Excellent mechanical property of the anti-compression or high collapse pressure has become an essential feature of new coronary stents. How to determine the design parameters of stent becomes the key to improve the stent quality. An integrated approach using radial basis function neural network （RBFNN） and genetic algorithm （GA） for the optimization of anti-compression mechanical property of stent is presented in this paper. First, finite element simulation and RBFNN are used to map the complex non-linear relationship between the collapse pressure and stent design parameters. Then GA is employed with the fitness function based on an RBFNN model for arriving at optimum configuration of the stent by maximizing the collapse pressure. The results of numerical experiment demonstrate that the combination of RBFNN and GA is an effective approach for the mechanical properties optimization of stent.

关键词： Stent Anti-compression Genetic algorithm （GA） Radial basis function neural network Optimization