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A single mutation in the PBC loop of VP2 is involved in the in vitro replication of infectious bursal disease virus

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Science China(Life Sciences) 2016年第7期59卷 717-723页

作者： Xiaole Qi xiang gao Zhen Lu Lizhou Zhang Yongqiang Wang Li gao Yulong gao Kai Li Honglei gao Changjun Liu Hongyu Cui Yanping Zhanga Xiaomei Wang Division of Avian Infectious Diseases State Key Laboratory of Veterinary Biotechnology Harbin Veterinary Research Institute Chinese Academy of Agricultural Sciences Harbin 150001 China Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Disease and Zoonoses Yangzhou 225009 China

To test whether amino acid mutations in the PBC and PHI loops of VP2 are involved in the replication and virulence of infectious bursal disease virus(IBDV), a pair of viruses, namely the moderately virulent IBDV(rG x-F9VP2) and the attenuated strain(rGt), were used. Residue mutations A222P(P_(BC)) and S330R(PHI), selected by sequence comparison, were introduced individually into r Gx-F9VP2 by using a reverse genetics system. In addition, the reverse mutation of either P222 A or R330 S was introduced into r Gt. The four modified viruses were then rescued and evaluated in vitro(CEF cells) and in vivo(SPF chickens). Results showed that A222 P elevated the replication efficiency of rG x-F9VP2 while P222 A reduced that of rG t in CEF cells. A mutation at residue 330 did not alter IBDV replication. In addition, animal experiments showed that a single mutation at either residue 222 or 330 did not significantly influence the virulence of IBDV. In conclusion, residue 222 in PBC of VP2 is involved in the replication efficiency of IBDV in vitro but does not affect its virulence in vivo, further facilitating our understanding of the gene-function of IBDV.

关键词：传染性法氏囊病病毒体外复制 PBC VP2 变参可靠性增长试验 IBDV 中等毒力

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催化烟机振动值上升原因分析与处理措施研究

现代制造技术与装备 2024年第1期60卷 154-156,169页

作者：高翔中国石油化工股份有限公司洛阳分公司洛阳471000

催化烟机在日常运行中振动值突升导致主风机联锁停机。通过逐一分析影响烟机振动的各种因素,查找出问题出现的原因,制定机组操作指南。经烟机解体检修后确认,此次烟机故障原因为拉杆断裂,为类似故障处理提供了经验。

关键词：烟气轮机振动值转子不平衡粉尘结垢

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装配式建筑结构防渗漏常见问题及管控措施

建设监理 2024年第5期 69-71,102页

作者：高翔上海建科工程咨询有限公司上海200032

随着我国城市化进程的加速发展和国家政策的支撑,装配式建筑在国内得到了广泛的应用。主要针对装配式结构的渗漏常见问题和防渗漏的管控措施进行研究,重点研究装配式结构各关键部位连接和预留预埋的防渗漏的管控等。分析装配式建筑出现... 详细信息

随着我国城市化进程的加速发展和国家政策的支撑,装配式建筑在国内得到了广泛的应用。主要针对装配式结构的渗漏常见问题和防渗漏的管控措施进行研究,重点研究装配式结构各关键部位连接和预留预埋的防渗漏的管控等。分析装配式建筑出现水平接缝渗漏、竖向接缝渗漏、预留预埋渗漏和窗框边缘等渗漏情况的原因,通过采取深化设计、构件加工、现场安装等相应管理措施,提高装配式的防渗漏效果和装配式建筑施工质量,减少渗漏水的风险。

关键词：装配式建筑防渗漏管控

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樱桃萝卜阳台种植技术

长江蔬菜 2024年第6期 60-62页

作者：高翔郑州市农业科技研究院河南郑州450015

樱桃萝卜是一种小型萝卜,一年四季均可栽培,其生长周期短,适应性强,管理简单,非常适宜在阳台种植。主要介绍了樱桃萝卜在阳台种植的关键技术,希望可为广大种植爱好者提供一些参考建议。

关键词：樱桃萝卜阳台种植技术

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浅谈通风安全监测监控系统现状

能源与节能 2024年第6期 284-286,290页

作者：高翔霍州煤电集团有限责任公司李雅庄煤矿山西霍州031400

煤矿通风安全监测监控系统在煤矿安全生产中发挥着至关重要的作用。近些年来,随着中国煤矿开采深度的增加和开采强度的增加,通风安全监测监控系统面临着一些挑战。首先,分析了通风安全监测监控系统面临的问题,主要包括监控数据形式单一... 详细信息

煤矿通风安全监测监控系统在煤矿安全生产中发挥着至关重要的作用。近些年来,随着中国煤矿开采深度的增加和开采强度的增加,通风安全监测监控系统面临着一些挑战。首先,分析了通风安全监测监控系统面临的问题,主要包括监控数据形式单一、调节控制功能不强以及报警分析能力不强等。然后,探讨了一些完善通风安全监测监控系统的对策,主要是增强系统的控制调节功能、引入智能分析决策以及提升安全监测的自动化水平等。可以为煤矿安全生产提供一定的技术参考。

关键词：煤矿通风安全生产监测监控

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一种适用多类型半导体基块的对中焊接工装

科技创新与应用 2024年第12期14卷 40-44页

作者：高翔中科艾尔(北京)科技有限公司北京100000

为提升半导体基块与堵帽在焊接过程中的质量精度,提高生产效率,减轻操作者的劳动强度,设计一款工装在生产过程中解决半导体基块流道孔因加工精度影响而出现焊缝质量不稳定问题并解决一种焊接工装可满足多种类半导体基块的焊接需求,提升... 详细信息

为提升半导体基块与堵帽在焊接过程中的质量精度,提高生产效率,减轻操作者的劳动强度,设计一款工装在生产过程中解决半导体基块流道孔因加工精度影响而出现焊缝质量不稳定问题并解决一种焊接工装可满足多种类半导体基块的焊接需求,提升生产效率。半导体基块焊接质量主要由半导体气路流道和堵帽配合同轴度与焊缝质量来评价,在此工装设计采用同轴对中校正结构实现一种可对中定心调整的焊接工装,并通过更换不同种类的垫块以用于多种类半导体基块与堵帽的对中焊接。通过工装的设计可以减少人工校对辅助时间,保证焊缝质量和批量生产需求。此焊接工装在实际使用中比人工焊接效率提升1倍并且焊缝美观,焊接质量可靠。

关键词：焊缝同轴度焊接工装工装定位焊缝修磨半导体焊接

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篮球技术训练与篮球意识的培养探究

冰雪体育创新研究 2024年第3期 185-187页

作者：高翔西安体育学院运动训练学院陕西榆林710068

篮球是一项需要高度协同和技巧的团队运动,其中,技术和意识是决定球员表现的两大关键因素。技术训练旨在提高球员的基本动作技能,而篮球意识则涉及在比赛中如何迅速做出决策、与队友协同工作,并预测对方的动作。该文详细探讨了如何分阶... 详细信息

篮球是一项需要高度协同和技巧的团队运动,其中,技术和意识是决定球员表现的两大关键因素。技术训练旨在提高球员的基本动作技能,而篮球意识则涉及在比赛中如何迅速做出决策、与队友协同工作,并预测对方的动作。该文详细探讨了如何分阶段、分层次地进行篮球技术训练,同时也介绍了培养篮球意识的关键方法。

关键词：篮球技术训练意识培养

维普期刊数据库博看期刊

Latest progress on fully non-fused electron acceptors for high-performance organic solar cells

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中国化学快报:英文版 2023年第8期34卷 76-81页

作者： Jianhong gao Xiaodong Zhu Hanyi Bao Jibao Feng xiang gao Zhitian Liu Ziyi Ge Hubei Engineering Technology Research Center of Optoelectronic and New Energy Materials Hubei Key Laboratory of Plasma Chemistry and Advanced MaterialsSchool of Materials Science and EngineeringWuhan Institute of TechnologyWuhan 430205China Ningbo Institute of Materials Technology and Engineering Chinese Academy of SciencesNingbo 315201China Center of Materials Science and Optoelectronics Engineering University of Chinese Academy of SciencesBeijing 100049China

Benefitting from the development of non-fullerene acceptors(NFAs),remarkable advances have been achieved with the power conversion efficiency(PCE)exceeding 19%over the last few years.However,the major achievement comes from fused ring electron acceptors(FREAs)with complex structures,leading to high cost.Hence,it is urgent to design new materials to resolve the cost issues concerning basic commercial requirements of organic solar cells.Recently,great progress has been made in fully non-fused ring electron acceptors(NFREAs)with only single-aromatic ring in the electron-donating core,which might achieve a fine balance between the efficiency and cost,thus accelerating the commercial application of organic solar cells.Therefore,this article summarizes the recent advances of fully NFREAs with efficiency over 10%,which may provide a guidance for developing the cost-effective solar cells.

关键词： Organic solar cells Fully non-fused electron acceptors Noncovalent interaction Power conversion efficiency Over 10%

Enhancing reversibility of LiNi_(0.5)Mn_(1.5)O_(4)by regulating surface oxygen deficiency

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Carbon Energy 2023年第11期5卷 81-89页

作者： Dandan Wang Cong gao Xuefeng Zhou Shang Peng Mingxue Tang Yonggang Wang Lujun Huang Wenge Yang xiang gao Center for High Pressure Science and Technology Advanced Research(HPSTAR) BeijingChina School of Materials Science and Engineering Harbin Institute of TechnologyHarbinChina

Oxygen deficiency has crucial effects on the crystal structure and electrochemical performance of spinel oxide lithium electrode materials such as LiNi_(0.5)Mn_(1.5)O_(4)(LNMO)cathode.In particular,the oxygen stoichiometry on the crystal surface differs from that on the crystal interior in LNMO.The detection of local oxygen loss in LNMO and its correlation with the crystal structure and the cycling stability of LNMO remain challenging.In this study,the effect of oxygen deficiency in LNMO controlled by sintering temperature on the surface crystal structure and electrochemical performance of LNMO is comprehensively investigated.The high concentration of oxygen vacancies segregates at the surface regions of LNMO forming a thin rock‐salt and/or deficient spinel surface layer.The atomic‐level surface structure reconstruction was demonstrated by annular dark‐field and annular brightfield techniques.For the synthesis of LNMO,the higher sintering temperature results in higher crystallinity but the higher oxygen deficiency in LNMO.The high crystallinity of LNMO would increase the thermal stability of LNMO cathodes while the high content of oxygen deficiency would decrease the surface structural stability of LNMO.Therefore,the LNMO sintered at a medium temperature of 850°C achieved the best capacity retention.The results suggest a competitive function mechanism between oxygen stoichiometry and the crystallinity of LNMO on the cycling performance of LNMO.

关键词： electrochemical performance LiNi_(0.5)Mn_(1.5)O_(4) lithium‐ion battery oxygen vacancies surface reconstruction