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Improvement of human embryonic stem cell-derived retinal pigment epithelium cell adhesion, maturation, and function through coating with truncated recombinant human vitronectin

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International Journal of Ophthalmology(English edition) 2021年第8期14卷 1160-1167页

作者： Xin-yue Zhu yu-Hong Chen Ting Zhang Su-Jun Liu Xin-yue Bai xian-yu huang Mei Jiang Xiao-Dong Sun Department of Ophthalmology Shanghai General HospitalShanghai Jiao Tong University School of MedicineShanghai 200080China National Clinical Research Center for Eye Diseases Shanghai 200080China Shanghai Key Laboratory of Fundus Diseases Shanghai 200080China Shanghai Engineering Center for Visual Science and Photomedicine Shanghai 200080China

AIM:To explore an xeno-free and defined coating substrate suitable for the culture of H9 human embryonic stem cell-derived retinal pigment epithelial(hES-RPE)cells in vitro,and compare the behaviors and functions of h ESRPE cells on two culture substrates,laminin521(LN-521)and truncated recombinant human vitronectin(VTN-N).METHODS:hES-RPE cells were used in the *** abilities of LN-521 and VTN-N at different concentrations to adhere to hES-RPE cells were compared with a high-content imaging *** real-time polymerase chain reaction was used to evaluate RPE-specific gene expression levels midway(day 10)and at the end(day 20)of the time *** polarity was observed by immunofluorescent staining for apical and basal markers of the *** phagocytic ability of hES-RPE cells was identified by flow cytometry and ***:The cell adhesion assay showed that the ability of LN-521 to adhere to hES-RPE cells was *** increasing coating concentration,an increasing number of cells attached to the surface of LN-521-coated *** contrast,VTN-N presented a strong adhesive ability even at a low *** optimal concentration of LN-521 and VTN-N required to coat and adhesion to hES-RPE cells were 2 and 0.25μg/cm^(2),***,both LN-521 and VTN-N could facilitate adoption of the desired cobblestone cellular morphology with tight junction and showed polarity by the hES-RPE ***,hES-RPE cells cultivated in VTN-N had a greater phagocytic ability,and it took less time for these hES-RPE cells to ***:VTN-N is a more suitable coating substrate for cultivating hES-RPE cells.

关键词： retinal pigment epithelial stem cell surface coating laminin vitronectin cell adhesion phagocytosis

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Infection within 2 weeks before liver transplantation closely related to prognosis of posttransplant infection:A single-center retrospective observational study in China

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Hepatobiliary & Pancreatic Diseases International 2020年第4期19卷 358-364页

作者： yue Ying Rui-Dong Li Jing-Wen Ai Yi-Min Zhu xian Zhou Yi-Yi Qian Xin-Chang Chen Xu-Yang Wang Hao-Cheng Zhang Yang Li Shan-Shan Weng Yi-Qi yu Yi-Feng Tao yu-xian huang Zheng-Xin Wang Wen-Hong Zhang Department of Infectious Diseases Huashan HospitalFudan UniversityShanghai 200040China Department of General Surgery Huashan HospitalFudan UniversityShanghai 200040China

Background:Infections still represent the main factors influencing morbidity and mortality following liver *** study aimed to evaluate the incidence and risk factors for infection and survival after liver ***:We retrospectively examined medical records in 210 liver recipients who underwent liver transplantation between April 2015 and October 2017 in our *** manifestations and results of pathogen detection test were used to define *** analyzed the prevalence,risk factors and prognosis of patients with ***:The median follow-up was 214 days;the incidence of infection after liver transplantation was 46.7%(n=98)which included pneumonia(43.4%),biliary tract infection(21.9%),peritonitis(21.4%)and bloodstream infection(7.6%).Among the pathogens in pneumonia,the most frequently isolated was Acinetobacter baumanii(23.5%)and Klebsiella pneumoniae(21.2%).Model for end-stage liver disease(MELD)score(OR=1.083,95%CI:1.045–1.123;P<0.001),biliary complication(OR=4.725,95%CI:1.119–19.947;P=0.035)and duration of drainage tube(OR=1.040,95%CI:1.007–1.074;P=0.017)were independent risk factors for posttransplant ***-cause mortality was 11.0%(n=23).The prognostic factors for postoperative infection in liver recipients were prior-transplant infection,especially pneumonia within 2 weeks before ***-Meier curves of survival showed that recipients within 2 weeks prior infection had a significantly lower cumulative survival rate compared with those without infection(65.2%vs.90.0%;hazard ratio:4.480;P<0.001).Conclusions:Infection,especially pneumonia within 2 weeks before transplantation,complication with impaired renal function and MELD score after 7 days of transplantation was an independent prognostic factor for postoperative infection in liver transplant recipients.

关键词： Infection Liver transplantation Risk factors MELD score

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Primary pulmonary amebic abscess in a patient with pulmonary adenocarcinoma:a case report

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Infectious Diseases of Poverty 2018年第1期7卷 344-348页

作者： yuan-yuan Liu yue Ying Chong Chen yue-Kai Hu Fei-Fei Yang Ling-yun Shao Xun-Jia Cheng yu-xian huang Department of Infectious Diseases Huashan HospitalFudan University12Middle Wulumuqi RoadShanghai 200040China Department of Infectious Diseases Chongqing Kaixian People’s Hospital8Ankang RoadChongqing 405499China Department of Medical Microbiology and Parasitology School of Basic Medical SciencesFudan University130 Dongan RoadShanghai 200032China

Background:Primary pulmonary amoeba is very rare and here we report a case of a 68-year-old man presenting with primary pulmonary amoeba after undergoing chemotherapy for lung *** presentation:In October 2016,the man aged 68 was admitted to our hospital because of repeated cough for 8 months and hemoptysis for 1 *** was diagnosed lung adenocarcinoma and underwent surgery in 2012 without receiving *** March 2016,the patients suffered recurrence of cancer and was treated with *** 2 months of chemotherapy,the patient had consistent cough with white sputum,and chest CT showed a local lung *** physicians suspected that the patient had pulmonary infectious diseases,and he was treated with empirical antibacterial ***,his symptom wasn’t relieved and later the percutaneous lung biopsy found trophozites of Entamoeba *** administration of metronidazole,the symptoms of the patient were markedly relieved and the lesions were ***:In such cases where patients with pulmonary nodules were in immunodeficiency state and had adequate but ineffective anti-bacterial treatment,Entamoeba histolytica infection could be one of the rare *** lung biopsy should be recommended and specific dying for parasites should be done when necessary.

关键词： Amebiasis Pulmonary amebic abscess Pulmonary adenocarcinoma

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Clinical features of acute hepatitis E super-infections on chronic hepatitis B

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World Journal of Gastroenterology 2016年第47期22卷 10388-10397页

作者： Chong Chen Shu-Ye Zhang Dan-Dan Zhang Xin-Yan Li yu-Ling Zhang Wei-Xia Li Jing-Jing Yan Min Wang Jing-Na Xun Chuan Lu yun Ling yu-xian huang Liang Chen Wenzhou Medical University Wenzhou 325000 Zhejiang Province China Shanghai Public Health Clinical Center Fudan University Shanghai 201508 China Institutes of Biomedical Sciences Fudan UniversityShanghai 200433 China School of Life Sciences Hebei Normal University Shijiazhuang 050000 Hebei Province China

AIM To examine the clinical features and risk factors for adverse outcomes in chronic hepatitis B(CHB) superimposed with hepatitis E virus(HEV).METHODS This retrospective cohort study included 228 patients with acute HEV infection(showing clinical acute hepatitis symptomology and positivity for anti-HEV immunoglobulin M) with underlying CHB(confirmed by positivity for hepatitis B surface antigen and/or hepatitis B virus(HBV) DNA over 6 mo) who had been admitted to the Shanghai Public Health Clinical Center, which represents the regional tertiary hospital for infectious diseases in Shanghai city, China. Data for adverse outcomes were collected, and included severe liver diseases(defined as liver failure and/or acute liver decompensation) and liver-related mortality. Logistic regression modeling was performed to determine the risk factors for adverse *** The symptoms caused by superimposed acute hepatitis E(AHE) were much more severe in cirrhotic patients(n = 94) than in non-cirrhotic patients(n = 134), as evidenced by significantly higher liver complications(77.7% vs 28.4%, P < 0.001) and mortality rate(21.3% vs 7.5%, P = 0.002). Most of the cirrhotic patients(n = 85, 90.4%) had no prior decompensation. Among the non-cirrhotic patients, superimposed AHE caused progressively more severe diseases that corresponded with the CHB disease stages, from immune tolerant to immune reactivation phases. Few risk factors were identified in the cirrhotic patients, but risk factors for non-cirrhotic patients were found to be intermediate HBV DNA levels(OR: 5.1, P = 0.012), alcohol consumption(OR: 6.4, P = 0.020), and underlying diabetes(OR: 7.5, P = 0.003) and kidney diseases(OR: 12.7, P = 0.005). Only 28.7% of the cirrhotic patients and 9.0% of the non-cirrhotic patients had received anti-HBV therapy previously and, in all cases, the efficacy had been suboptimal. CONCLUSION CHB-related cirrhosis and intermediate HBV DNA level were associated with severe disease in sup

关键词： Cirrhosis Co-infections Liver failure Liver decompensation Stages of hepatitis B virus infection

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试论“黎人”对苏轼诗文创作之影响

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中南民族大学学报（人文社会科学版） 2007年第1期27卷 160-163页

作者：鲜于煌重庆师范大学文学与新闻学院重庆400047

本文详细论述了海南少数民族“黎人”对苏轼诗文创作的重大影响。文章指出,这对更加全面地认识苏轼沉浮宦海、驰骋翰墨、波澜起伏的一生和诗文之创作,显然有不可忽视的重要意义和作用。

关键词：少数民族“黎人” 苏轼诗文创作影响

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An unexpected Diels-Alder type cycloaddition of cyclic enone ethylene ketals

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Chinese Journal of Chemistry 1993年第4期11卷 350-359页

作者： CHOW,yuan L. CHENG,xian-En ZHANG,yu-huang Department of Chemistry,Simon Fraser University,Burnaby,B.C.Canada V5A 1S6 Department of Chemistry Simon Fraser University Burnaby B. C. Canada V5A iSe

The ethylene ketal of 2-cyelopentenone and 2-cyelohexenone in acetonitrile in the presence of a trace of an acid rapidly undergoes the Diels-Alder type dimerization,The products are regiospecific but not stereoselective and are hydrolysed sequentially to afford keto-ketals and diketones whose structures are determined by extensive applications of NMR spectroscopy.

关键词： An unexpected Diels-Alder type cycloaddition of cyclic enone ethylene ketals type

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Solution of rate constants in pharmacokinetics local model of nuclear medicine

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Nuclear Science and Techniques 1998年第3期 58-60页

作者： Cao Guo xian, yu Hui Xin and huang Xu Quan ( State Key Laboratory of Nuclear Medicine, Jiangsu Institute of Nuclear Medicine, Wuxi 214063)

１ＩｎｔｒｏｄｕｃｔｉｏｎＩｔｉｓｏｂｖｉｏｕｓｔｈａｔｎｏｒｍａｌｐｈａｒｍａｃｏｋｉｎｅｔｉｃｓｍｏｄｅｌ，ｓｕｃｈａｓｔｈｅｃｏｍｐａｒｔｍｅｎｔｍｏｄｅｌ，ｃａｎｏｎｌｙｄｅｓｃｒｉｂｅｐｈａｒｍａｃｅｕｔｉ... 详细信息

１ＩｎｔｒｏｄｕｃｔｉｏｎＩｔｉｓｏｂｖｉｏｕｓｔｈａｔｎｏｒｍａｌｐｈａｒｍａｃｏｋｉｎｅｔｉｃｓｍｏｄｅｌ，ｓｕｃｈａｓｔｈｅｃｏｍｐａｒｔｍｅｎｔｍｏｄｅｌ，ｃａｎｏｎｌｙｄｅｓｃｒｉｂｅｐｈａｒｍａｃｅｕｔｉｃａｌｓ’ｐｈａｒｍａｃｏｋｉｎｅ...

关键词： Pharmacokinetics Local model Rate constants

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Improving the accuracy of laser particle sizer with Mie scattering theory

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Chinese Journal of Lasers 1992年第5期 41-46页

作者： Gang Zheng, xian-huang yu, Nai-ning Wang (Shanghai Institute of Mechanical Engineering,shanghai,China)

Using the classical Mie scattering theory and Fraunhofer diffraction theory,adetailed analysis of the differences of the light energy distribution falling on the multi-elementconcentric photo-detector of a diffraction based laser particle sizer is *** calcula-tions and computer simulation are carried out. Experimental studies are also made with latexspheres as Standard Reference *** research shows that when the classical Mie scat-tering theory is used,the accuracy of the particle sizer can be essentially improved not only inthe small size range,but also for large *** the same time the time needed for data re-duction in both cases is almost the same.

关键词： laser particle sizer Mie scattering theory

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基于DEA的物流配送中心再选址研究

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数学的实践与认识 2017年第19期47卷 197-202页

作者：张于贤黄鑫桂林电子科技大学商学院广西桂林541004

为实现现有物流配送中心的优化管理的目的,利用DEA方法对已有配送中心的效率进行评估.根据评估的结果代入选址分析模型中,得到物流配送中心再选址的DEA评估规划模型,以配送收益与物流成本之差作为目标函数,以供需均衡关系、配送中心流... 详细信息

为实现现有物流配送中心的优化管理的目的,利用DEA方法对已有配送中心的效率进行评估.根据评估的结果代入选址分析模型中,得到物流配送中心再选址的DEA评估规划模型,以配送收益与物流成本之差作为目标函数,以供需均衡关系、配送中心流出量与流入量平衡、选址变量与流量内在联系构建约束条件,通过算例并借助于MATLAB软件验证了模型的有效性.

关键词： DEA方法规划模型物流配送中心选址均衡关系

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The role of prophylactic antibiotics in hepatitis B virus-related acute-on-chronic liver failure patients at risk of bacterial infection: a retrospective study

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Infectious Diseases of Poverty 2021年第2期10卷 84-84页

作者： Xiao-Qin Liu Xue-yun Zhang yueYing Jian-Ming Zheng Jian Sun Wen-Hong Zhang Ji-Ming Zhang yu-xian huang Departm ent of Infectious Diseases Huashan HospitalFudan University12 Middle Urumqi RoadShanghai 200040China 不详

Background:Acute-on-chronic liver failure(ACLF)is characterized by an excessive systemic inflammatory response and organ failure and has high *** infections(Bis)worsen the clinical course of ACLF and carry a poor prognosis in ACLF *** efficacy of third-generation cephalosporins has been challenged in recent *** aim of this study was to characterize the difference between ACLF patients with and without Bis and to provide a reference for medical intervention.

关键词： Acute-on-chronic liver failure Hepatitis B virus Infection Prophylactic antibiotics

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