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Aerobic exercise improves ejaculatory behaviors and complements dapoxetine treatment by upregulating the BDNF-5-HT duo:a pilot study in rats

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Asian Journal of Andrology 2023年第5期25卷 637-642页

作者： Yuan-Yuan Huang Dang-Wei Peng Qiu-Shi Liu Hui Jiang xian-sheng zhang Department of Urology The First Affiliated Hospital of Anhui Medical UniversityHefei 230022China Department of Urology Peking University First HospitalBeijing 100000China Andrology Center Peking University First HospitalBeijing 100000China Institute of Urology Peking UniversityBeijing 100000China

Accumulating evidence has revealed many clues that regular aerobic exercise benefits brain health and behaviors.The aims of this study were to explore the effect of aerobic exercise on ejaculatory behaviors,as well as to make a preliminary assessment of aerobic exercise as a complementary strategy to dapoxetine treatment in rapid ejaculators.Copulatory tests of rats and a treadmill training protocol were performed in this study.In total,12 rapid ejaculators were selected on the basis of ejaculation distribution theory and randomly assigned to 4 groups:control(Ctrol)group,aerobic exercise(Ex)group,dapoxetine(Dapo)group,and Ex+Dapo group.We evaluated the changes in ejaculatory parameters in the 4 groups.Variations in biological markers,including serum corticosterone,serotonin(5-HT),and brain-derived neurotrophic factor(BDNF)of the raphe nucleus,were determined by enzyme-linked immunosorbent assay(ELISA).The primary finding of our study was that both aerobic exercise and acute dapoxetine could enhance ejaculation control and prolong ejaculation latency in rapid ejaculator rats.The ejaculation delay effect of aerobic exercise was nearly equivalent to that of acute dapoxetine.In addition,both aerobic exercise and dapoxetine treatment could lead to increased expression of BDNF and 5-HT in the raphe nucleus of rapid ejaculators.Moreover,the two interventions,when applied together,may further upregulate the expression of BDNF-5-HT duo in a complementary manner.This study highlights the positive effects of aerobic exercise on ejaculation control.Regular aerobic exercise might be a promising complementary treatment to dapoxetine in rats.

关键词： aerobic exercise animal model brain-derived neurotrophic factor premature ejaculation raphe nucleus serotonin

MBOAT1 homozygous missense variant causes nonobstructive azoospermia

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Asian Journal of Andrology 2022年第2期24卷 186-190页

作者： Yang-Yang Wan Lan Guo Yao Yao Xiao-Yun Shi Hui Jiang Bo Xu Juan Hua xian-sheng zhang Department of Urology The First Affiliated Hospital of Anhui Medical UniversityHefei 230022China Department of Biochemistry and Molecular Biology School of Basic Medical SciencesAnhui Medical UniversityHefei 230032China The Department of Urology Peking University Third HospitalPeking 100191China Reproductive and Genetic Hospital The First Affiliated Hospital of USTCDivision of Life Sciences and MedicineUniversity of Science and Technology of ChinaHefei 230001China

Nonobstructive azoospermia(NOA)is a common cause of infertility and is defined as the complete absence of sperm in ejaculation due to defective spermatogenesis.The aim of this study was to identify the genetic etiology of NOA in an infertile male from a Chinese consanguineous family.A homozygous missense variant of the membrane-bound O-acyltransferase domain-containing 1(MBOAT1)gene(c.770C>T,p.Thr257Met)was found by whole-exome sequencing(WES).Bioinformatic analysis also showed that this variant was a pathogenic variant and that the amino acid residue in MBOAT1 was highly conserved in mammals.Quantitative polymerase chain reaction(Q-PCR)analysis showed that the mRNA level of MBOAT1 in the patient was 22.0%lower than that in his father.Furthermore,we screened variants of MBOAT1 in a broader population and found an additional homozygous variant of the MBOAT1 gene in 123 infertile men.Our data identified homozygous variants of the MBOAT1 gene associated with male infertility.This study will provide new insights for researchers to understand the molecular mechanisms of male infertility and will help clinicians make accurate diagnoses.

关键词： male infertility membrane-bound O-acyltransferase domain-containing 1 nonobstructive azoospermia whole-exome sequencing

Towards an understanding of plant reproductive isolation:uncovering the species-specific signal for pollen tube guidance

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Science China(Life Sciences) 2020年第4期63卷 620-622页

作者： Xin-Qi Gao Huan Guan xian-sheng zhang National Key Laboratory of Crop Biology College of Life SciencesShandong Agricultural UniversityTai’an 271018China

Fertilization produces diploid zygote as the initiation of new diploid organism by fusing two haploid gametes(sperm and egg cell).Different from animals,plants in angiosperms produce immobile sperms in pollen/pollen tube(male gametophyte).Whereas,the egg cell is located in the female gametophyte(embryo sac)that consists of two female gametes(egg and central cells)and accessory cells(synergids and antipodals).Embryo sac is embedded in the ovule of ovary,which is an enlarged basal portion of the pistil.The growth of pollen tube provides a strategy for the immobile sperm approaching the egg cell.After pollination,pollen germinates at the surface of the stigma and produces pollen tube.It grows through the transmitting tract of the style and emerges from it to enter the ovary in response to certain attracting cues from the ovule.

关键词： attracting approaching tube

Regulation of cell reprogramming by auxin during somatic embryogenesis

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aBIOTECH 2020年第3期1卷 185-193页

作者： Li Ping Tang xian sheng zhang Ying Hua Su State Key Laboratory of Crop Biology College of Life SciencesShandong Agricultural UniversityTai’an 271018ShandongChina

How somatic cells develop into a whole plant is a central question in plant developmental biology.This powerful ability of plant cells is recognized as their totipotency.Somatic embryogenesis is an excellent example and a good research system for studying plant cell totipotency.However,very little is known about the molecular basis of cell reprogramming from somatic cells to totipotent cells in this process.During somatic embryogenesis from immature zygotic embryos in Arabidopsis,exogenous auxin treatment is required for embryonic callus formation,but removal of exogenous auxin inducing endogenous auxin biosynthesis is essential for somatic embryo(SE)induction.Ectopic expression of specific transcription factor genes,such as "LAFL" and BABY BOOM(BBM),can induce SEs without exogenous growth regulators.Somatic embryogenesis can also be triggered by stress,as well as by disruption of chromatin remodeling,including PRC2-mediated histone methylation,histone deacetylation,and PKL-related chromatin remodeling.It is evident that embryonic identity genes are required and endogenous auxin plays a central role for cell reprogramming during the induction of SEs.Thus,we focus on reviewing the regulation of cell reprogramming for somatic embryogenesis by auxin.

关键词： Somatic embryogenesis Auxin Cell reprogramming Transcription factors Chromatin remodeling

Initiation and maintenance of plant stem cells in root and shoot apical meristems

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aBIOTECH 2020年第3期1卷 194-204页

作者： Junxia Wang Yinghua Su xiangpei Kong Zhaojun Ding xian sheng zhang The Key Laboratory of Plant Development and Environmental Adaptation Biology Ministry of EducationCollege of Life SciencesShandong UniversityQingdao 266237ShandongChina State Key Laboratory of Crop Biology College of Life SciencesShandong Agricultural UniversityTai’an 271018ShandongChina

Plant stem cells are a small group of cells with a self-renewal capacity and serve as a steady supply of precursor cells to form new differentiated tissues and organs in plants.Root stem cells and shoot stem cells,which are located in the root apical meristem and in the shoot apical meristem,respectively,play a critical role in plant longitudinal growth.These stem cells in shoot and root apical meristems remain as pluripotent state throughout the lifespan of the plant and control the growth and development of plants.The molecular mechanisms of initiation and maintenance of plant stem cells have been extensively investigated.In this review,we mainly discuss how the plant phytohormones,such as auxin and cytokinin,coordinate with the key transcription factors to regulate plant stem cell initiation and maintenance in root and shoot apical meristems.In addition,we highlight the common regulatory mechanisms of both root and shoot apical meristems.

关键词： Stem cell Initiation Maintenance Auxin Cytokinin Transcription factors

Plant cell totipotency: Insights into cellular reprogramming

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Journal of Integrative Plant Biology 2021年第1期63卷 228-240页

作者： Ying Hua Su Li Ping Tang xiang Yu Zhao xian sheng zhang State Key Laboratory of Crop Biology College of Life SciencesShandong Agricultural UniversityTai'an 271018China

Plant cells have a powerful capacity in their propagation to adapt to environmental change, given that a single plant cell can give rise to a whole plant via somatic embryogenesis without the need for fertilization. The reprogramming of somatic cells into totipotent cells is a critical step in somatic embryogenesis. This process can be induced by stimuli such as plant hormones, transcriptional regulators and stress. Here, we review current knowledge on how the identity of totipotent cells is determined and the stimuli required for reprogramming of somatic cells into totipotent cells. We highlight key molecular regulators and associated networks that control cell fate transition from somatic to totipotent cells. Finally,we pose several outstanding questions that should be addressed to enhance our understanding of the mechanisms underlying plant cell totipotency.

关键词： cell reprogramming plant cell totipotency somatic embryogenesis totipotent cell

Meristem Biology Flourishes Under Mt. Tai

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Molecular Plant 2016年第8期9卷 1224-1227页

作者： Yuling Jiao Olivier Hamant Zhaojun Ding xian sheng zhang State Key Laboratory of Plant Genomics Institute of Genetics and Developmental Biology Chinese Academy of Sciences National Research Beijing 100101 China Laboratoire Reproduction et D6veloppement des Plantes Univ Lyon ENS de Lyon UCB Lyon 1 CNRS INRA 69342 Lyon France Ministry of Education Key Laboratory of Plant Cell Engineering and Germplasm Innovation College of Life Sciences Shandong University Jinan Shandong 250100 China State Key Laboratory of Crop Biology College of Life Sciences Shandong Agricultural University Tai'an Shandong 271018 China These authors contributed equally to this article.

The green and lush Mt. Tai provided an appropriate backdrop for the First International Conference on Plant Meristem Biology held on June 9 and 10, 2016. Proposed and organized by xian sheng zhang （Shandong Agricultural University, China）, Jiayang Li （Insti- tute of Genetics ＆ Developmental Biology, China）, Elliot Meyero- witz （California Institute of Technology, USA）, and Yuling Jiao （Institute of Genetics ＆ Developmental Biology, China）, the con- ference was hosted by Shandong Agricultural University and sponsored by the Department of Education, Shandong Province, China. The first meeting of its kind, it brought about 300 attendees from nine countries together for discussions on molecular mechanisms underlying shoot, root, cambium,

关键词： International Proposed Developmental

Single-cell profiling lights different cell trajectories in plants

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aBIOTECH 2021年第1期2卷 64-78页

作者： Hong-xiang Zheng Feng-hui Wu Si-min Li xian sheng zhang Na Sui Shandong Provincial Key Laboratory of Plant Stress College of Life SciencesShandong Normal UniversityJi’nan 250014ShandongChina State Key Laboratory of Crop Biology College of Life SciencesShandong Agricultural UniversityTai’an 271018ShandongChina

The molecular mechanism of the maintenance and differentiation of plant stem cells is an eternal theme in studies on plant growth and development.Recent advances in single-cell RNA sequencing(scRNAseq)methods have completely changed the understanding of cell heterogeneity and cell function,allowing research precision to identify the differentiation trajectory of stem cells maintained and differentiated at the cellular level.This review aimed to mainly discuss the novel insights provided by scRNA-seq for the maintenance and initiation of plant stem cells,cell differentiation,cell response to environmental changes,and improvement strategies for scRNA-seq.In addition,it highlighted additional perspectives beyond scRNA-seq,such as spatial transcriptomes,epigenomes,and single-cell multiomics,for a renewed understanding of stem cell maintenance and cell differentiation,thus providing potential targets and theoretical foundations for crop improvement.

关键词： Epigenomes Plant development Plant stem cells Single-cell multi-omics Single-cell RNA sequencing

Validity of premature ejaculation diagnostic tool and its association with International Index of Erectile Function-15 in Chinese men with evidence-based-defined premature ejaculation

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Asian Journal of Andrology 2018年第1期20卷 19-23页

作者： Dong-Dong Tang Chao Li Dang-Wei Peng xian-sheng zhang Department of Urology The First Affiliated Hospital of Anhui Medical University Hefei China Reproductive Medicine Center The First Affiliated Hospital of Anhui Medical University Hefei China

The premature ejaculation diagnostic tool （PEDT） is a brief diagnostic measure to assess premature ejaculation （PE）. However, there is insufficient evidence regarding its validity in the new evidence-based-defined PE. This study was performed to evaluate the validity of PEDT and its association with IIEF-15 in different types of evidence-based-defined PE. From June 2015 to January 2016, a total of 260 men complaining of PE and defined as lifelong PE （LPE）/acquired PE （APE） according to the evidence-based definition from Andrology Clinic of the First Affiliated Hospital of Anhui Medical University, along with 104 male healthy controls without PE from a medical examination center, were enrolled in this study. All individuals completed questionnaires including demographics, medical and sexual history, as well as PEDT and IIEF-15. After statistical analysis, it was found that men with PE reported higher PEDT scores （14.28 ± 3.05） and lower IIEF-15 （41.26 ± 8.20） than men without PE （PEDT： 5.32 ± 3.42, IIEF-15：52.66 ± 6.86, P 〈 0.001 for both）. It was suggested that a score of 〉9 indicated PE in both LPE and APE by sensitivity and specificity analyses （sensitivity： 0.875, 0.913; specificity： 0.865, 0.865, respectively）. In addition, IIEF-15 were higher in men with LPE （42.64 ± 8.11） than APE （39.43 ± 7.84, P 〈 0.001）. After adjusting for age, IIEF-15 was negatively related to PEDT in men with LPE （adjust r = -0.225, P 〈 0.001） and APE （adjust r = -0.378, P 〈 0.001）. In this study, we concluded that PEDT was valid in the diagnosis of evidenced-based-defined PE. Furthermore, IIEF-15 was negatively related to PEDT in men with different types of PE.

关键词： erectile dysfunction International Index of Erectile Function-15 male sexual dysfunction premature ejaculation premature ejaculation diagnostic tool