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Amphibian pore-forming proteinβγ-CAT drives metabolite release from small extracellular vesicles through channel formation

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Zoological Research 2023年第4期44卷 739-742页

作者： Qi-Quan Wang Xin-Qiang Lan Xue-Song Wei Si-Man Xu ling-Zhen Liu xian-ling bian Lin Zeng Xiao-Long Guo Ying-Qi Guo Wen-Hui Lee Yang xiang Yun Zhang Human Aging Research Institute(HARI)and School of Life Science Nanchang Universityand Jiangxi Key Laboratory of Human AgingNanchangJiangxi 330031China Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences&Yunnan Province Kunming Institute of ZoologyChinese Academy of SciencesKunmingYunnan 650201China Center for Excellence in Animal Evolution and Genetics Chinese Academy of SciencesKunmingYunnan 650201China Institutional Center for Shared Technologies and Facilities of the Kunming Institute of Zoology Chinese Academy of SciencesKunmingYunnan 650201China

DEAR EDITOR,Pore-forming proteins(PFPs)are widely distributed among all kingdoms of life and can oligomerize to form pores/channels in membrane *** vesicles(EVs)circulate in all biological fluids and can trigger biological responses at a distance,thus emerging as an additional means of intercellular communication through the release of cellular cargo,such as lipids,nucleic acids,metabolites,and *** date,however,the mechanism by which EV contents are released into extracellular space remains *** our previous study on toads(Bombina maxima),we identified a PFP and trefoil factor complexβγ-CAT(βγ-crystallin fused aerolysin-like protein(α-subunit)and trefoil factor(β-subunit)complex,hence namedβγ-CAT),which assembled under strict regulation in response to environmental ***,we observed that the PFPβγ-CAT colocalized with EVs in the skin of *** in *** small EVs(sEVs)isolated from *** dermal fibroblast cells and murine fibroblast cells,we found thatβγ-CAT could specifically target and oligomerize on purified EVs,rather than disrupt membrane integrity,and promote the release of different *** analysis revealed that a secretory PFP drove metabolite release from EVs through channel formation,providing novel clues for the delivery of EV content into extracellular space.

关键词： release extracellular hence

Purification, characterization and functional analysis of peptidyl-aminoacyl-L/D-isomerase from Bombina maxima

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Purification, characterization and functional analysis of pe...

ImSpiRE: image feature-aided spatial resolution enhancement method

中国生物化学与分子生物学会2019年全国学术会议暨学会...

作者： xian-ling bian Qi-Quan Wang ling-Zhen Liu Wen-Hui Lee Yun Zhang Xiao-Long Guo School of Life Science University of Science and Technology of China Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences/Key Laboratory of Bioactive Peptides of Yunnan Province Kunming Institute of Zoology Chinese Academy of Sciences School of Clinical Medicine Weifang Medical University

Although most natural proteins are composed exclusively of L-configuration amino acids, more and more emerging evidences showed that D-amino acids play crucial roles in sustaining the functions of proteins or peptides, as well as in the development of diseases. Peptidyl-aminoacyl-L/D-isomerase is a key enzyme to catalyze the transition from L-amino acids to D-amino acids. However, the molecular characterization and functional analysis of the enzyme in amphibian species of Bombina maxima were unclear. In this study, the enzyme was purified from skin secretions of B. maxima. The apparent molecular weight of the purified enzyme was determined to be 35 kDa. Interestingly, no internal or interchain disulfide bonds was found for this enzyme. Functional study revealed that the isomerase has a strong ability to catalyzes the isomerization of an L-Ile in position 2 of a model peptide(IIGPVLGC) to D-allo-Ile. Mutation study demonstrated that the lysine in position122 of the enzyme was essential for its function. Interestingly, a D-amino acids containing peptide named maximin H was identified from B. maxima skin secretions and displayed a strong antimicrobial activity. However, the activity of maximin H was significant decreased once the its D-Ile changed to L-Ile. These findings suggested that peptidyl-aminoacyl-L/D-isomerase was vital for sustaining the functions of frog-derived bio-active peptides and the survival of frogs.

关键词： peptidyl-aminoacyl-L/D-isomerase D-amino acid Bombina maxima

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Science China Life Sciences 2025年第1期 272-283页

作者： Yuwei Hua Yizhi Zhang Zhenming Guo Shan bian Yong Zhang State Key Laboratory of Cardiovascular Diseases and Medical Innovation Center Institute for Regenerative MedicineDepartment of NeurosurgeryShanghai East HospitalShanghai Key Laboratory of Signaling and Disease ResearchFrontier Science Center for Stem Cell ResearchSchool of Life Sciences and TechnologyTongji University

The resolution of most spatially resolved transcriptomic technologies usually cannot attain the single-cell level, limiting their applications in biological discoveries. Here, we introduce ImSpiRE, an image feature-aided spatial resolution enhancement method for in situ capturing spatial transcriptome. Taking the information stored in histological images, ImSpiRE solves an optimal transport problem to redistribute the expression profiles of spots to construct new transcriptional profiles with enhanced resolution, together with extending the gene expression profiles into unmeasured regions. Applications to multiple datasets confirm that ImSpiRE can enhance spatial resolution to the subspot level while contributing to the discovery of tissue domains, signaling communication patterns, and spatiotemporal characterization.

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Cohort study on the treatment of BRAF V600E mutant metastatic colorectal cancer with integrated Chinese and western medicine

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World Journal of Clinical Oncology 2025年第1期16卷 25-33页

作者： Jiang-Yu bian Yu-Fang Feng Wen-Ting He Tong Zhang Department of Oncology Xiyuan Hospital of China Academy of Chinese Medical SciencesBeijing 100091China Department of Oncology The Fourth Clinical Medical College of Xinjiang Medical UniversityUrumqi 830001China Department of Oncology Traditional Chinese Medicine Hospital Affiliated to Xinjiang Medical UniversityUrumqi 830001China

BACKGROUND Patients with BRAF V600E mutant metastatic colorectal cancer(mCRC)have a low incidence rate,poor biological activity,suboptimal response to conventional treatments,and a poor *** the previous cohort study on mCRC conducted by our team,it was observed that integrated Chinese and Western medicine treatment could significantly prolong the overall survival(OS)of patients with colorectal ***,we further explored the survival benefits in the population with BRAF V600E mutant *** To evaluate the efficacy of integrated Chinese and Western medicine in the treatment of BRAF V600E mutant metastatic colorectal *** A cohort study was conducted on patients with BRAF V600E mutant metastatic colorectal cancer admitted to Xiyuan Hospital of China Academy of Chinese Medical Sciences and Traditional Chinese Medicine Hospital of Xinjiang Uygur Autonomous Region from January 2016 to December *** patients were divided into two *** A total of 34 cases were included,with 23 in Chinese-Western medicine cohort(cohort A)and 11 in Western medicine cohort(cohort B).The median overall survival was 19.9 months in cohort A and 14.2 months in cohort B,with a statistically significant difference(P=0.038,hazard ratio=0.46).The 1-3-year survival rates were 95.65%(22/23),39.13%(9/23),and 26.09%(6/23)in cohort A,and 63.64%(7/11),18.18%(2/11),and 9.09%(1/11)in cohort B,*** analysis showed statistically significant differences in median OS between the two cohorts in the right colon,liver metastasis,chemotherapy,and first-line treatment subgroups(P<0.05).CONCLUSION Integrated Chinese and Western medicine can prolong the survival and reduce the risk of death in patients with BRAF V600E mutant metastatic colorectal cancer,with more pronounced benefits observed in patients with right colon involvement,liver metastasis,combined chemotherapy,and first-line treatment.

关键词： Metastatic colorectal cancer BRAF V600E mutation Integrated Chinese and Western medicine Cohort study

An Improved Method of Simultaneous Determination of Four Bioactive Compounds in Evodiae Fructus Using Ionic Liquids as Mobile Phase Additives in High Performance Liquid Chromatography

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Chemical Research in Chinese Universities 2017年第4期33卷 552-558页

作者： bian Min TIAN ling YAO Cheng College of Chemistry and Molecular Engineering Nanj'ing Tech University Nanjing 211800 P. R. China

There are many compounds with different structures and chemical properties in Evodiae Fructus. It is hard to simultaneously determine the bioactive compounds by high performance liquid chromatography（HPLC）. A new method was proposed for four bioactive compounds（synephrine, limonoids, evodiamine and rutecarpine） to be separated completely and determined accurately using ionic liquids（ILs） as mobile phase additives. The mechanism and the effect of the ILs for changing the chromatographic behaviors of the four compounds were studied by systemati- cally changing the pH value of mobile phase, the types and concentrations of ILs as well as the concentrations of phosphate buffer. The chromatographic behaviors of the analytes with a mobile phase containing ILs complied with the stoichiometric displacement model for retention（SDM-R）. All results demonstrate the dual nature of ionic liquids, which are competitive adsorption and ion-pair agent. Meanwhile, excellent linearity was observed for all the com- pounds with correlation coefficients between 0.9992 and 0.9998. The limit of detection and the limit of quantification of the four compounds varied from 0.47μg/mL to 0.87 μg/mL and from 1.79 μg/mL to 2.44μg/mL, respectively. Three kinds of Evodiae Fructus processed through different methods were analyzed via the method. The result shows that the contents of evodiamine and rutecarpine as the two main active compounds by processing with vinegar and salt are obviously higher than those of the raw products.

关键词： Evodiae Fructus Ionic liquid Mobile phase additive High performance liquid chromatography(HPLC) Processed Evodiae Fructus

Cobalamin-dependent radical S-adenosyl-L-methionine protein functions with a partner to successively methylate tricyclic indole alkaloid for chuangxinmycin maturation and derivatization

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Science China Chemistry 2025年第1期 308-316页

作者： Sili Wang Jiancheng Huang Yanan Du Wei Huang Yufeng Xue Xiaokun Xu Guannan Zhong Yuanyuan Shi Bin Hong Xiaoying bian Wen Liu State Key Laboratory of Chemical Biology Shanghai Institute of Organic Chemistry University of Chinese Academy of SciencesChinese Academy of Sciences State Key Laboratory of Microbial Technology Shandong University National Health Commission (NHC) Key Laboratory of Biotechnology for Microbial Drugs Institute of Medicinal BiotechnologyChinese Academy of Medical Science & Peking Union Medical College

Cobalamin(Cbl)-dependent radical S-adenosyl-L-methionine(SAM) proteins constitute the largest collection of the radical SAM superfamily that has hundreds of thousands of individual members. Many of these proteins are involved in the biosynthesis of pharmaceutically important natural products to catalyze chemically demanding reactions. In the biosynthetic pathway of chuangxinmycin(CXM), a unique indole alkaloid antibiotic with potent anti-infective activity, functionalization of the characteristic thiopyrano[4,3,2-cd]indole scaffold by regio-and stereoselective C3-methylation is believed to rely on a Cbl-dependent radical process, which, however, remained to be reconstituted biochemically. We here report the dissection of this enzymatic process, which requires the incorporation of Cxm8, a Cbl-dependent radical SAM protein, with Cxm9, a DUF5825family protein that shares no homology to any proteins of known functions. Cxm8 and Cxm9 function together by forming an unexpected heterodimeric complex that selectively catalyzes C3-methylation of the tricyclic indole-S-hetero ring system in a successive manner, achieving CXM and a recently identified, C3-dimethylated congener. Detailed biochemical characterization,isotope labeling, structural simulation and bioinformatics analysis rationalized the catalysis of the Cxm8/Cxm9 complex and particularly the necessity of the DUF5825 protein for C3-methylase activity. This is the first example that a Cbl-dependent protein acts with a partner to exhibit radical SAM activity.

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Numerical analysis of heat transfer enhancement on steam condensation in the presence of air outside the tube

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Nuclear Science and Techniques 2022年第8期33卷 55-68页

作者： Wen-Tao Li xian-Ke Meng Hao-Zhi bian Ming Ding Heilongjiang Provincial Key Laboratory of Nuclear Power System and Equipment Harbin Engineering UniversityHarbin 150001China China Nuclear Engineering Consulting Co. Ltd.Beijing 100073China

In loss-of-coolant accidents,a passive containment heat removal system protects the integrity of the containment by condensing *** a large amount of air exists in the containment,the steam condensation heat transfer can be significantly *** on previous research,traditional methods for enhancing pure steam condensation may not be applicable to steam–air *** the present study,new methods of enhancing condensation heat transfer were adopted and several potentially enhanced heat transfer tubes,including corrugated tubes,spiral fin tubes,and ring fin tubes were ***-CCM+was used to determine the effect of enhanced heat transfer tubes on the steam condensation heat *** to the calculations,the gas pressure ranged from 0.2 to 1.6 MPa,and air mass fraction ranged from 0.1 to *** effective perturbation of the high-concentration air layer was identified as the key factor for enhancing steam–air condensation heat ***,the designed corrugated tube performed well at atmospheric pressure,with a maximum enhancement of 27.4%,and performed poorly at high *** the design of spiral fin tubes,special attention should be paid to the locations that may accumulate high-concentration ***,the ring-fin tubes generally displayed good performance under all conditions of interest,with a maximum enhancement of 24.2%.

关键词： Air–steam condensation Numerical simulation Heat transfer enhancement Fin tube

Desulfurization Characteristics of CaO-SiO_2-BaO-CaF_2-Al_2O_3-MgO Refining Slag

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Journal of Iron and Steel Research International 2005年第5期12卷 1-2,20页

作者： GAO Yan-hong bian ling-tao Chongqing University of Science and Technology Chongqing 400005 China lingyuan Iron and Steel Group Co Lingyuan 122504 China

The desulfurization characteristics of CaO-SiO2-BaO-CaF2-Al2O3-MgO refining slag were studied in laboratory with the methodology of orthogonal design. The influence of basicity, Al2O3, MgO, BaO and CaF2 contents in sl... 详细信息

关键词： refining slag desulfurization orthogonal design

Identification of a novel gain-of-function mutant allele,slr1-d5,of rice DELLA protein

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Journal of Integrative Agriculture 2016年第7期15卷 1441-1448页

作者： ZHANG Yun-hui bian Xiao-feng ZHANG Suo-bing ling Jing WANG Ying-jie WEI Xiao-ying FANG xian-wen The Jiangsu Provincial Platform for Conservation and Utilization of Agricultural Germplasm/Jiangsu Provincial Key Laboratory of Agrobiology/Institute of Food Crops Jiangsu Academy of Agricultural SciencesNanjing 210014P.R.China Shandong Business Institute Yantai 264670P.R.China

Controling the height of crops plays a crucial role for their yields. The large scale utilization of semi-dwarf varieties has greatly improved crop yield, providing an effective support for world food security. In rice, a main food for over half of the world’s population, a number of dwarf loci have been identiifed. However, most of them are recessive, such as the ‘green revolution’ genesd1. To gain more beneifcial loci for rice breeding programs, exploring new mutations is needed, especialy the dominant loci which can be used broadly for hybrid breeding. Here, we isolated a novel dominant dwarf rice mutant, slr1-d5. Al of the internodes ofslr1-d5are reduced. We ifnd that the responsiveness ofslr1-d5to gibberelin （GA）, GA3, was signiifcantly reduced. Map-based cloning revealed that the dominant dwarifsm ofslr1-d5was caused by an amino acid substitution in the N-terminal TVHYNP domain of rice DELLA protein, SLR1, where the conserved amino acid Pro （P） was substituted to His （H）. Our ifndings not only further prove the pivotal role of TVHYNP motif in regulating SLR1 stability, but also provide a new dwarf source for improvement of rice germplasms.

关键词： rice dominant dwarf DELLA protein gibberelin