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Telomere-to-telomere genome of the allotetraploid legume Sesbania cannabina reveals transposon-driven subgenome divergence and mechanisms of alkaline stress tolerance

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Science China(Life Sciences) 2024年第1期67卷 149-160页

作者： Haofei Luo Xiaofei Wang Changqing You Xuedan Wu Duofeng Pan Zhiyao Lv Tong Li Dongmei Zhang Zhongbao Shen Xiaodong Zhang Guodao Liu Kaixuan He Qingtong Ye Yajun Jia Qinghua zhao xian Deng Xiaofeng Cao xianwei Song Gai Huang State Key Laboratory of Plant Genomics and National Center for Plant Gene Research Institute of Genetics and Developmental BiologyChinese Academy of SciencesBeijing 100101China Hainan Yazhou Bay Seed Lab Sanya 572025China University of Chinese Academy of Sciences Beijing 100049China Institute of Forage and Grassland Sciences Heilongjiang Academy of Agricultural SciencesHarbin 150086China Shandong Academy of Agricultural Sciences Jinan 250100China National Center of Technology Innovation for Comprehensive Utilization of Saline-Alkali Land Dongying 257345China Shandong Green Manure Ecological Technology Co. LtdDongying 257345China State Key Laboratory of Tropical Crop Breeding Chinese Academy of Tropical Agricultural SciencesHaikou 571101China

Alkaline soils pose an increasing problem for agriculture worldwide,but using stress-tolerant plants as green manure can improve marginal ***,we show that the legume Sesbania cannabina is very tolerant to alkaline conditions and,when used as a green manure,substantially improves alkaline *** understand genome evolution and the mechanisms of stress tolerance in this allotetraploid legume,we generated the first telomere-to-telomere genome assembly of *** spanning~2,087 *** assembly included all centromeric regions,which contain centromeric satellite repeats,and complete chromosome ends with telomeric *** genome analysis distinguished A and B subgenomes,which diverged approximately 7.9 million years *** genomic analysis revealed that the chromosome homoeologs underwent large-scale inversion events(>10 Mb)and a significant,transposon-driven size expansion of the chromosome 5A *** further identified four specific alkali-induced phosphate transporter genes in ***;these may function in alkali tolerance by relieving the deficiency in available phosphorus in alkaline *** work highlights the significance of *** as a green tool to improve marginal lands and sheds light on subgenome evolution and adaptation to alkaline soils.

关键词： Sesbania cannabina alkaline stress telomere-to-telomere genome legume green manure phosphate transporter allotetraploid

Large‑scale transcriptomic and genomic analyses reveal a novel functional gene SERPINB6 for chicken carcass traits

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Journal of Animal Science and Biotechnology 2024年第4期15卷 1466-1481页

作者： Di zhao Ranran Liu Xiaodong Tan Huimin Kang Jie Wang Zheng Ma Haiquan zhao Hai xiang Zhengfen Zhang Hua Li Guiping zhao Guangdong Provincial Key Laboratory of Animal Molecular Design and Precise Breeding School of Life Science and EngineeringFoshan UniversityFoshanChina State Key Laboratory of Animal Biotech Breeding Institute of Animal SciencesChinese Academy of Agricultural SciencesBeijingChina Guangdong Tinoo’s Foods Group Co. Ltd.QingyuanChina

Background Carcass traits are crucial indicators of meat production ***,the molecular regulatory mechanisms associated with these traits remain *** In this study,we conducted comprehensive transcriptomic and genomic analyses on 399 Tiannong partridge chickens to identify key genes and variants associated with carcass traits and to elucidate the underlying regulatory *** on association analyses with the elastic net(en)model,we identified 12 candidate genes(AMY1A,AP3B2,CEBPG,EEF2,EIF4EBP1,FGFR1,FOXD3,GOLM1,LOC107052698,PABPC1,SERPINB6 and TBC1D16)for 4 carcass-related traits,namely live weight,dressed weight,eviscerated weight,and breast muscle ***6 was identified as the only overlapping gene by 3 analyses,en model analysis,weighted gene co-expression network analysis and differential expression ***-level experiments confirmed that SERPINB6 promotes the proliferation of chicken DF1 cells and primary *** expression genome-wide association study and association analysis indicated that rs317934171 is the critical site that enhances SERPINB6 ***,a dual-luciferase reporter assay proved that gga-miR-1615 targets the 3′UTR of *** Collectively,our findings reveal that SERPINB6 serves as a novel gene for chicken carcass traits by promoting fibroblast and myoblast ***,the downstream variant rs317934171 regulates SERPINB6 *** results identify a new target gene and molecular marker for the molecular mechanisms of chicken carcass traits.

关键词： Carcass traits Chicken Genome SERPINB6 Transcriptome

电弧离子镀Al-Cr-O/Zr-O+NiCoCrAlSiY和Al-Cr-O+NiCoCrAlSiY涂层的抗氧化性和隔热性能

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Transactions of Nonferrous Metals Society of China 2024年第4期34卷 1237-1250页

作者：鲜丽君赵海波范洪远鲜广四川大学机械工程学院成都610065 四川大学分析测试中心成都610065

采用电弧离子镀在高温合金表面沉积Al-Cr-O/Zr-O多层涂层和Al-Cr-O单层涂层+NiCoCrAlSiY结合层,并在1000~1200℃下进行热处理。利用扫描电子显微镜和X射线衍射仪分析涂层的显微组织和物相结构。结果表明,Al-Cr-O/Zr-O多层涂层和Al-Cr-O... 详细信息

采用电弧离子镀在高温合金表面沉积Al-Cr-O/Zr-O多层涂层和Al-Cr-O单层涂层+NiCoCrAlSiY结合层,并在1000~1200℃下进行热处理。利用扫描电子显微镜和X射线衍射仪分析涂层的显微组织和物相结构。结果表明,Al-Cr-O/Zr-O多层涂层和Al-Cr-O单层涂层均呈现出致密的球形结构。热处理后,Al-Cr-O/Zr-O涂层表面出现裂纹,且裂纹随着温度的升高而增多和变粗。然而,Al-Cr-O涂层经热处理后其表面胞状结构转变为紧密连接的粒状结构,并且随着热处理温度的升高,粒状结构显著长大。由于作为氧离子导体以及t-ZrO_(2)的致密度低于α-Al_(2)O_(3),Al-Cr-O/Zr-O涂层中的t-ZrO_(2)为氧离子向涂层内扩散提供了通道,因此,Al-Cr-O单层涂层+NiCoCrAlSiY涂层体系的高温抗氧化性优于Al-Cr-O/Zr-O多层涂层+NiCoCrAlSiY涂层体系。但是,由于更大的陶瓷涂层厚度,t-ZrO_(2)相的低热导率以及层间界面的热反射作用,Al-Cr-O/Zr-O多层涂层+NiCoCrAlSiY涂层体系的隔热性能优于Al-Cr-O单层涂层+NiCoCrAlSiY涂层体系。

关键词：电弧离子镀 Al-Cr-O/Zr-O多层涂层抗氧化性隔热性能

In situ forming injectable MSC-loaded GelMA hydrogels combined with PD for vascularized sweat gland regeneration

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Military Medical Research 2024年第1期11卷 152-155页

作者： enhe Jirigala Bin Yao zhao Li Yi-Jie Zhang Chao Zhang Li-Ting Liang Fan-Liang Zhang Xing-Yu Yuan xian-Lan Duan Wei Song Meng-De Zhang Yi Kong Xiao-Bing Fu Sha Huang Research Center for Tissue Repair and Regeneration Medical Innovation Research DepartmentChinese PLA General Hospital and PLA Medical CollegeBeijing 100048China Institute of Basic Medical Research Inner Mongolia Medical UniversityHohhot 010110China Academy of Medical engineering and Translational Medicine Tianjin UniversityTianjin 300072China School of Medicine Nankai UniversityTianjin 300071China

Dear Editor,Three dimensional(3D)bioprinted extracellular matrix(ECM)can be used to provide both biochemical and biophysical cues to direct mesenchymal stem cells(MSCs)differentiation,and then differentiated cells were isolated for implantation in vivo using surgical ***,the reduced cell activity after cell isolation from 3D constructs and low cell retention in injured sites limit its application[1].Methacrylated gelatin(GelMA)hydrogel has the advantage of fast crosslinking,which could resemble complex architectures of tissue construct in vivo[2].Here,we adopted a noninvasive bioprinting procedure to imitate the regenerative microenvironment that could simultaneously direct the sweat gland(SG)and vascular differentiation from MSCs and ultimately promote the replacement of glandular tissue in situ(Fig.1a).

关键词： Sweat gland GelMA In situ niche Cell differentiation Tissue incorporation Vascularization

长江中游通江故道水环境特征及其与浮游植物群落的关联分析

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长江流域资源与环境 2024年第7期33卷 1540-1549页

作者：刘小光柴朝晖朱孔贤水利部长江中下游河湖治理与防洪重点实验室湖北武汉430015 长江水利委员会长江科学院湖北武汉430015

为了厘清长江中游通江故道水环境特征及其对浮游植物群落的影响,在汛前及汛后对长江中游通江故道进行了水环境及浮游植物调查,包括TP、TN、NH_(3)-N、COD_(Mn)、BOD_(5)等水质指标以及浮游植物的种类组成、生物量、生物多样性等。通过... 详细信息

为了厘清长江中游通江故道水环境特征及其对浮游植物群落的影响,在汛前及汛后对长江中游通江故道进行了水环境及浮游植物调查,包括TP、TN、NH_(3)-N、COD_(Mn)、BOD_(5)等水质指标以及浮游植物的种类组成、生物量、生物多样性等。通过构建优化的模糊数学评价方法,对各故道水质状况及汛前汛后变化进行了量化评价,并对各故道富营养化状态进行评估,利用RDA分析与灰色关联分析探究了影响浮游植物群落特征的主要水质指标。结果表明:(1)各故道水质基本为III~IV,在汛前闸控通江故道水质要好于自然通江故道,在汛后则相反;(2)影响故道浮游植物密度的主要水质指标为TP与COD_(Mn),影响故道浮游植物群落多样性的水质指标主要为TN、NH_(3)-N与COD_(Mn);(3)建议通过营养盐阻控、水位控制与水动力调控等措施修复故道水质、优化浮游植物多样性与丰富度。

关键词：长江中游通江故道水环境浮游植物模糊数学

Effect of negative remodeling of the side branch ostium on the efficacy of a two-stent strategy for distal left main bifurcation lesions:an intravascular ultrasound study

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Journal of Geriatric Cardiology 2024年第5期21卷 506-522页

作者： Yi XU Tian XU Jia-Cong NONG Xiao-Han KONG Meng-Yao zhao Zhi-Jing GAO Yi-Fei WANG Wei YOU Pei-Na MenG Yu-He ZHOU xiang-Qi WU Zhi-Ming WU Mei-en ZHAN Yan-Qing WANG De-Feng PAN Fei YE Department of Cardiology Nanjing First HospitalNanjing Hospital Affiliated to Nanjing Medical UniversityNanjing Medical UniversityNanjingChina Department of Cardiology Clinical Medicine School of Nanjing UniversityNanjingChina Department of Cardiology Tianchang People’s HospitalTianchangChina Department of Cardiology the Affiliated Hospital of Xuzhou Medical UniversityXuzhouChina

OBJECTIVES To investigate whether negative remodeling(NR) detected by intravascular ultrasound(IVUS) of the side branch ostium(SBO) would affect in-stent neointimal hyperplasia(NIH) at the one-year follow-up and the clinical outcome of target lesion failure(TLF) at the long-term follow-up for patients with left main bifurcation(LMb) lesions treated with a two-stent *** A total of 328 patients with de novo true complex LMb lesions who underwent a 2-stent strategy of percutaneous coronary intervention(PCI) treatment guided by IVUS were enrolled in this study. We divided the study into two phases. Of all the patients, 48 patients who had complete IVUS detection pre-and post-PCI and at the 1-year follow-up were enrolled in phase Ⅰ analysis, which aimed to analyze the correlation between NR and in-stent NIH at SBO at the 1-year follow-up. If the correlation was confirmed, the cutoff value of the remodeling index(RI) for predicting NIH ≥ 50% was analyzed next. The phase Ⅱ analysis focused on the incidence of TLF as the primary endpoint at the 1-to 5-year follow-up for all 328 patients by grouping based on the cutoff value of *** In phase I: according to the results of a binary logistic regression analysis and receiver operating characteristic(ROC) analysis, the RI cutoff value predicting percent NIH ≥ 50% was 0.85 based on the ROC curve analysis, with a sensitivity of 85.7%, a specificity of 88.3%, and an AUC of 0.893(0.778, 1.000), P = 0.002. In phase Ⅱ: the TLR rate(35.8% vs. 5.3%, P ent NIH post-PCI for distal LMb lesions with a 2-stent strategy,and NR with RI ≤ 0.85 is linked to percent NIH area ≥ 50% at the 1-year follow-up and more TLF at the 5-year follow-up.

关键词： lesions remodeling intravascular

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一种有状态容器跨集群实时迁移方法

计算机研究与发展 2024年第2期61卷 494-502页

作者：吴宪汤红波赵宇许明艳解放军信息工程大学郑州450001

虚拟容器的跨集群实时迁移技术是网络服务云化应用中的一项重要技术.容器实时迁移技术的优化能够达到缩短云端服务停机时间的目的,在实际应用中有重要的现实意义.目前针对跨集群容器实时迁移优化的研究相对较少,其中迭代迁移是一种能够... 详细信息

虚拟容器的跨集群实时迁移技术是网络服务云化应用中的一项重要技术.容器实时迁移技术的优化能够达到缩短云端服务停机时间的目的,在实际应用中有重要的现实意义.目前针对跨集群容器实时迁移优化的研究相对较少,其中迭代迁移是一种能够有效缩短停机时间的迁移方式,但这项技术在实际应用中还存在很多需要解决的问题.针对跨集群场景中网络延迟对服务停机时间产生的影响提出了一种容器实时迁移方法,该方法着重考虑集群间的网络延迟,并据此建立了数学模型.通过数学模型理论分析了跨集群迁移过程中网络延迟对迁移性能的影响,确定了启用迭代迁移方法的起始条件和迭代过程的终止条件,并给出了计算满足终止条件迭代次数的方法.该模型充分考虑了网络延迟对跨集群容器实时迁移的影响.实验结果表明该方法能够有效缩短服务停机时间.

关键词：容器实时迁移网络延迟服务停机时间迭代迁移跨集群

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青少年道德决策的同伴影响及合理性促进

教育理论与实践 2024年第14期44卷 37-40页

作者：赵献梓河北师范大学教育学院河北石家庄050024

同伴在青少年心目中有着十分重要的地位,个体会通过同伴来凸显自身的独立性,也会依赖同伴满足某些情感需求。由于同伴在青少年学习生活中的重要地位,会较多地影响青少年的道德认知、决策情绪和决策思维。外因通过内因发挥作用,内因在事... 详细信息

同伴在青少年心目中有着十分重要的地位,个体会通过同伴来凸显自身的独立性,也会依赖同伴满足某些情感需求。由于同伴在青少年学习生活中的重要地位,会较多地影响青少年的道德认知、决策情绪和决策思维。外因通过内因发挥作用,内因在事物发展中起决定性作用。青少年道德决策合理化的关键在于夯实青少年道德认知,增加个道德决策的理性成分;传授青少年道德决策方法,夯实个体道德决策的智慧基础;加强青少年独立人格建设,增强同伴导向下个体决策自主性;利用同伴与青少年的情感联结,促使个体基于积极情感体验做出决策。

关键词：青少年道德决策同伴影响道德认知道德情感道德思维

基于尾灯灯语的混行交通流车辆驾驶意图识别模型研究

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重庆交通大学学报（自然科学版） 2024年第2期43卷 48-56页

作者：赵树恩赵东宇重庆交通大学机电与车辆工程学院重庆400074

针对长期存在自动驾驶车辆(autonomous vehicle,AV)与人工驾驶车辆(human-driven vehicle,HV)混行的交通现状,结合深度学习和HSV颜色特征提取方法,提出了一种在混行交通环境下基于尾灯灯语的车辆驾驶意图识别模型。以Mask R-CNN(mask re... 详细信息

针对长期存在自动驾驶车辆(autonomous vehicle,AV)与人工驾驶车辆(human-driven vehicle,HV)混行的交通现状,结合深度学习和HSV颜色特征提取方法,提出了一种在混行交通环境下基于尾灯灯语的车辆驾驶意图识别模型。以Mask R-CNN(mask region proposal convolutional neural network)车辆模型检测出的感兴趣区域RoI(region of interest)为限制,将区域内的HV作为研究对象,根据尾灯位置相关性,在HV车尾区域添加纵横向约束来定位传递灯语信号的左右尾灯;在规定的灯语组合及转向灯闪烁频率基础上,提出了一种基于时间序列的灯语识别算法,运用多目标判别相关性滤波CSRT(channel and spatial relatability tracking)跟踪HV尾灯并统计尾灯时序状态;以动态灯语作为输入,构建基于尾灯灯语的驾驶意图识别模型;使用基于真实路况信息的Cityspaces数据集和交通流视频数据对模型进行训练、验证和测试。研究结果表明:基于尾灯灯语的驾驶意图识别模型对视频流车辆尾灯检测准确率和召回率分别为96.0%、98.2%,对驾驶意图识别的平均准确率达到了95.9%,单帧识别耗时为20 ms,为高速混行环境下的AV决策规划提供了有效的理论依据。

关键词：交通工程驾驶意图识别灯语识别自动驾驶 Mask R-CNN 交互行为