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A transcriptional cascade involving BBX22 and HY5 finely regulates both plant height and fruit pigmentation in citrus

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Journal of Integrative Plant Biology 2024年第8期66卷 1752-1768页

作者： Jialing Fu Li Liao Jiajing Jin Zhihao Lu Juan Sun Lizhi Song Yue Huang Shengjun Liu Ding Huang Yuantao Xu Jiaxian He Bin Hu Yiqun Zhu Fangfang Wu xia Wang Xiuxin Deng Qiang Xu National Key Laboratory for Germplasm Innovation and Utilization of Horticultural Crops College of Horticulture and Forestry SciencesHuazhong Agricultural UniversityWuhan 430070P.R.China Hubei hongshan Laboratory Wuhan 430070P.R.China Science and Technology Innovation Research Center of Majia Pomelo Shangrao 334000China

Dwarfing is a pivotal agronomic trait affecting both yield and *** species exhibit substantial variation in plant height,among which internode length is a core ***,the molecular mechanism governing internode elongation remains ***,we unveiled that the transcriptional cascade consisting of B-BOX DOMAIN PROTEIN 22(BBX22)and ELONGATED HYPOCOTYL 5(HY5)finely tunes plant height and internode elongation in ***-of-function mutations of BBX22 in an early-flowering citrus(Citrus hindsii“SJG”)promoted internode elongation and reduced pigment accumulation,whereas ectopic expression of BBX22 in SJG,sweet orange(***),pomelo(***)or heterologous expression of BBX22 in tomato(Solanum lycopersicum)significantly decreased internode ***,exogenous application of gibberellin A3(GA3)rescued the shortened internode and dwarf phenotype caused by *** experiments revealed that BBX22 played a dual role in regulation internode elongation and pigmentation in *** the one hand,it directly bound to and activated the expression of HY5,GA metabolism gene(GA2 OXIDASE 8,GA2ox8),carotenoid biosynthesis gene(PHYTOENE SYNTHASE 1,PSY1)and anthocyanin regulatory gene(Ruby1,a MYB DOMAIN PROTEIN).On the other hand,it acted as a cofactor of HY5,enhancing the ability of HY5 to regulate target genes ***,our results reveal the critical role of the transcriptional cascade consisting of BBX22 and HY5 in controlling internode elongation and pigment accumulation in *** the crosstalk regulatory mechanism between internode elongation and fruit pigmentation provides key genes for breeding of novel types with both dwarf and health-beneficial fortification in citrus.

关键词： anthocyanins BBX22 carotenoids citrus HY5 internode elongation plant height

3D Co(II, III) mixed-valence metal-organic framework affording field-induced slow magnetic relaxation

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Science China Chemistry 2015年第6期58卷 1032-1038页

作者： Sheng Zhang xiangyu Liu Bin Liu Zhengqiang xia Weitao Wang Qi Yang hongshan Ke Qing Wei Gang Xie Sanping Chen Shengli Gao Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education College of Chemistry and Materials Science Northwest University School of Chemistry and Chemical Engineering Ningxia University College of Chemistry and Chemical Engineering Shaanxi University of Science & Technology

A 3D mixed-valence Co（Ⅲ）-Co（Ⅱ） compound [Co9（bta）10（Hbta）2（H2O）10]n·[22（H2O）]n （1）（H2bta=N,N-bis（1H-tetrazole-5- yl）-amine） was hydrothermally synthesized by reaction of Co（NO3）2·6H2O with H2bta·H2O. Compound 1 consists of three kinds of distorted-octahedral [CoⅡ（N4O2）] paramagnetic nodes which are separated by [CoⅢ（bta）2（Hbta）]2-/[CoⅢ（bta）3]3- dia- magnetic linkers to generate a 3D porous metal-organic framework （MOF） with alternative …Co（Ⅲ）…Co（II）… array and channels incorporating water molecules. Under an applied magnetic field of 4000 Oe, compound 1 exhibits slow relaxation of magnetization at low temperatures, giving AE/kB=30.O0 K and ι0=2.0×10^-8 s.

关键词： Co(Ⅱ, Ⅲ) mixed-valence metal-organic framework, slow magnetic relaxation

Characterization of a 4.1 Mb inversion harboring the stripe rust resistance gene YR86 on wheat chromosome 2AL

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The Crop Journal 2024年第4期12卷 1168-1175页

作者： Qiang Cao Zhanwang Zhu Dengan Xu Jianhui Wu xiaowan Xu Yan Dong Yingjie Bian Fugong Ding Dehui Zhao Yang Tu Ling Wu Dejun Han Caixia Lan xianchun xia Zhonghu He Yuanfeng Hao College of Agronomy Northwest A&F UniversityYangling 712100ShaanxiChina State Key Laboratory of Crop Gene Resources and Breeding Institute of Crop SciencesChinese Academy of Agricultural Sciences(CAAS)Beijing 100081China Food Crops Institute Hubei Academy of Agricultural Sciences/Wheat Disease Biology Research Station for Central ChinaWuhan 430064HubeiChina Crop Research Institute Sichuan Academy of Agricultural SciencesChengdu 610066SichuanChina Hubei hongshan Laboratory College of Plant Science and TechnologyHuazhong Agricultural UniversityWuhan 430070HubeiChina CIMMYT-China Office C/O CAASBeijing 100081China

Wheat cultivar Zhongmai 895 was earlier found to carry YR86 in an 11.6 Mb recombination-suppressed region on chromosome 2AL when crossed with Yangmai *** fine-map the YR86 locus,we developed two large F2 populations from crosses Emai 580/Zhongmai 895 and Avocet S/Zhongmai ***,both populations exhibited suppressed recombination in the same 2AL *** analysis across Chinese Spring,Aikang 58,and 10+wheat genomes revealed a 4.1 Mb chromosomal inversion spanning 708.5-712.6 Mb in the Chinese Spring reference *** markers were developed in the breakpoint and were used to assess a wheat cultivar panel,revealing that Chinese Spring,Zhongmai 895,and Jimai 22 shared a common sequence named InvCS,whereas Aikang 58,Yangmai 16,Emai 580,and Avocet S shared the sequence named *** inverted configuration explained the suppressed recombination observed in all three bi-parental *** recombination was observed in a Jimai 22/Zhongmai 895 F2 population,facilitating mapping of YR86 to a genetic interval of 0.15 cM corresponding to 710.27-712.56 Mb falling within the inverted ***-three high-confidence genes were annotated in the interval using the Chinese Spring reference genome,with six identified as potential candidates for YR86 based on genome and transcriptome *** results will accelerate map-based cloning of YR86 and its deployment in wheat breeding.

关键词： Adult-plant resistance Chromosomal inversion Puccinia striiformis Triticum aestivum

KLF4 facilitates chromatin accessibility remodeling in porcine early embryos

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Science China(Life Sciences) 2024年第1期67卷 96-112页

作者： Wei Zhu Guowei Bu Ruifeng Hu Jixiang Zhang Lianyong Qiao Kai Zhou Tingting Wang Qiao Li Jingjing Zhang Linhui Wu Yali Xie Taotao Hu Shichun Yang Jiaqi Guan xiaoyu Chu Juanjuan Shi xia Zhang Falong Lu Xin Liu Yi-Liang Miao Institute of Stem Cell and Regenerative Biology College of Animal Science and Veterinary MedicineHuazhong Agricultural UniversityWuhan 430070China Key Laboratory of Agricultural Animal Genetics Breeding and Reproduction(Huazhong Agricultural University)Ministry of EducationWuhan 430070China State Key Laboratory of Molecular Developmental Biology Institute of Genetics and Developmental BiologyInnovative Academy of Seed DesignChinese Academy of SciencesBeijing 100101China Frontiers Science Center for Animal Breeding and Sustainable Production Huazhong Agricultural UniversityWuhan 430070China Hubei hongshan Laboratory Wuhan 430070China

Chromatin accessibility remodeling driven by pioneer factors is critical for the development of early *** studies have illustrated several pioneer factors as being important for agricultural animals,but what are the pioneer factors and how the pioneer factors remodel the chromatin accessibility in porcine early embryos is not *** employing low-input DNase-seq(liDNase-seq),we profiled the landscapes of chromatin accessibility in porcine early embryos and uncovered a unique chromatin accessibility reprogramming pattern during porcine preimplantation *** data revealed that KLF4 played critical roles in remodeling chromatin accessibility in porcine early *** down of KLF4 led to the reduction of chromatin accessibility in early embryos,whereas KLF4 overexpression promoted the chromatin openness in porcine ***,KLF4 deficiency resulted in mitochondrial dysfunction and developmental failure of porcine *** addition,we found that overexpression of KLF4 in blastocysts promoted lipid droplet accumulation,whereas knockdown of KLF4 disrupted this *** together,our study revealed the chromatin accessibility dynamics and identified KLF4 as a key regulator in chromatin accessibility and cellular metabolism during porcine preimplantation embryo development.

关键词： porcine embryos liDNase-seq chromatin accessibility KLF4 mitochondrion lipid droplets

Hierarchical Coordinated Control for Power System Voltage Using Linear Temporal Logic

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Engineering（科研） 2009年第2期1卷 117-126页

作者： hongshan ZHAO Hongliang GAO Yang xia 不详

The paper proposed an approach to study the power system voltage coordinated control using Linear Temporal Logic (LTL). First, the hybrid Automata model for power system voltage control was given, and a hierarchical coordinated voltage control framework was described in detail. In the hierarchical control structure, the high layer is the coordinated layer for global voltage control, and the low layer is the power system controlled. Then, the paper introduced the LTL language, its specification formula and basic method for control. In the high layer, global voltage coordinated control specification was defined by LTL specification formula. In order to implement system voltage coordinated control, the LTL specification formula was transformed into hybrid Automata model by the proposed algorithms. The hybrid Automata in high layer could coordinate the different distributed voltage controller, and have constituted a closed loop global voltage control system satisfied the LTL specification formula. Finally, a simple example of power system voltage control include the OLTC controller, the switched capacitor controller and the under-voltage shedding load controller was given for simulating analysis and verification by the proposed approach for power system coordinated voltage control. The results of simulation showed that the proposed method in the paper is feasible.

关键词： Power Systems Voltage Control Linear Temporal Logic Hierarchical Coordinated Control Hybrid Automata

战略性新兴产业民营企业创新动力评测与比较分析——纳入隐性制度安排视角

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工业技术经济 2015年第5期34卷 20-28页

作者：艾洪山李侠湖南大学长沙410079

考虑到存在隐性制度安排视角下,企业创新动力评价指标多为定性指标和定量指标共同组成,且大多指标取值具有模糊不确定性的特征,本文基于模糊数学理论构造了民营企业创新动力多维关系矩阵,在此基础上,结合2005~2007年战略性新兴产业民营... 详细信息

考虑到存在隐性制度安排视角下,企业创新动力评价指标多为定性指标和定量指标共同组成,且大多指标取值具有模糊不确定性的特征,本文基于模糊数学理论构造了民营企业创新动力多维关系矩阵,在此基础上,结合2005~2007年战略性新兴产业民营企业数据,运用模糊综合评价法建立民营企业创新动力模糊评价模型,从一个全新的角度检验了隐性制度安排对民营企业创新动力的影响程度和渠道。结果显示,中国战略性新兴产业民营企业创新动力水平呈现出区域非均衡性分布,制度环境特别是隐性制度环境在中国战略性新兴产业民营企业创新动力的生成和发展过程中扮演着关键的角色。在此基础上,为优化制度环境,本文提出了加强对政策性金融工具的监督,加强监督机制,完善政府采购法律法规,使政府采购公平公正,严格减免税批准审核机制的政策建议。

关键词：创新动力隐性制度模糊决策民营企业

PEMFC水淹的阳极气体压力降变化特征预警技术

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农业机械学报 2014年第7期45卷 340-346页

作者：宋满存裴普成曾夏查鸿山清华大学汽车安全与节能国家重点实验室北京100084

通过观察阳极气体压力降对一个两片的质子交换膜燃料电池堆进行水淹预警的研究发现,其在水淹过程中阳极气体压力降具有'两级台阶'的变化特征。结合流道内水积聚过程的研究成果以及电压的变化特点,可以将水淹过程分为良好期、湿润期、过... 详细信息

通过观察阳极气体压力降对一个两片的质子交换膜燃料电池堆进行水淹预警的研究发现,其在水淹过程中阳极气体压力降具有'两级台阶'的变化特征。结合流道内水积聚过程的研究成果以及电压的变化特点,可以将水淹过程分为良好期、湿润期、过渡期和水淹期4个阶段。对比实验表明电流和温度对于两级台阶的相对幅度影响不大,气体压力和阳极过量系数对台阶相对幅度有较显著的影响。提高气体压力和增大阳极过量系数可以提高PEMFC抵抗水淹的能力,但增大阳极过量系数则会造成氢气浪费。调整电池堆工作温度是一种有效的水淹自愈手段,通过调整温度可以使PEMFC工作在'微湿未淹'的状态下。阳极脉冲排气或增大阳极气体过量系数则可作为PEMFC严重水淹时的辅助处理措施。

关键词：质子交换膜燃料电池水淹预警阳极气体压力降

Effects of Fuke Qianjin Formula on hormones and their receptors and metabonomics study in uterine fibroids model rats

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Digital Chinese Medicine 2021年第4期4卷 316-327页

作者： LI Yamei TANG Jie LUO hongshan xia Bohou LIN Limei LIAO Duanfang College of Pharmacy Hunan University of Chinese MedicineChangshaHunan 410208China Key Laboratory for Quality Evaluation of Bulk Herbs of Hunan Province ChangshaHunan 410208China

Objective To investigate the effects of different fractions from Fuke Qianjin Formula(妇科千金方,FKQJF)on uterine leiomyoma(UL)to determine the best *** FKQJF was extracted and isolated to obtain polysaccharides(FKP),flavonoids(FKF),and grease(FKG).140 female SPF SD rats were divided into 14 groups[model(MOD),normal control(NC),Gouliuqing(GLQ),Mifepristone(MFST),FKQJF,low,medium,and high dose of polysaccharides(l-FKP,m-FKP,and h-FKP),low,medium,and high dose of flavonoids(l-FKF,m-FKF,and h-FKF),low,medium,and high dose of grease(l-FKG,m-FKG,and h-FKG)],and uterine fibroids model rats were treated with drugs for four *** levels of estrogen and progesterone were measured using enzyme-linked immunoassay assay(ELISA)*** expression of estrogen receptor(ER-α,ER-β)and progesterone receptor(PR)in the uterus was observed using immunohistochemistry(IHC).Serum metabolite profiles and FKG were analyzed using gas chromatography-mass spectrometry(GC-MS).Results FKQJF,h-FKF,m-FKG,and h-FKG significantly downregulated the estrogen level in the uterine fibroid model rats(P<0.01).FKQJF,h-FKF,and h-FKG significantly reduced the level of progesterone in the uterine fibroid model rats(P<0.01).The levels of ER-α,ER-β,and PR in uterine fibroid model rats were significantly decreased by FKQJF and h-FKG(P<0.01).The levels of ER-α,ER-β,and PR in the fibroid model rats were decreased by m-FKG(P<0.05).Additionally,serum metabolism results revealed that h-FKG and FKQJF could regulate related endogenous metabolites and make the pathological indices of uterine fibroids in rats close to the normal ***-six components were identified in the oil,accounting for 91.97%of the total oil *** FKQJF and h-FKG showed a significant anti-myoma activity and significantly improved the pathological state of the uterus in rats with *** mechanism of action may be related to the regulation of estrogen progesterone and its receptor in uterine fibroid model a

关键词： Fuke Qianjin Formula(妇科千金方,FKQJF) Uterine leiomyoma Grease Gas chromatography-mass spectrometry(GCMS) Metabolomics Estrogen Progesterone Estrogen receptor Progesterone receptor

超高海拔地区足月新生儿出生体质量及影响因素

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武汉大学学报（医学版） 2021年第2期42卷 296-301页

作者：张漪骆红山彭斯聪夏世文旦巴扎西湖北省妇幼保健院新生儿科湖北武汉430070 湖北省中西医结合医院病案统计科湖北武汉430030 西藏自治区山南市妇幼保健院儿科西藏山南856100

目的:了解平均海拔3 700 m地区的藏族足月新生儿的平均出生体质量及其与平原地区同胎龄新生儿的差别,以及发生低出生体质量儿(LBWI)的危险因素。方法:回顾性收集2018年9月1日至2019年9月1日在西藏山南市妇幼保健院产科出生的足月新生儿... 详细信息

目的:了解平均海拔3 700 m地区的藏族足月新生儿的平均出生体质量及其与平原地区同胎龄新生儿的差别,以及发生低出生体质量儿(LBWI)的危险因素。方法:回顾性收集2018年9月1日至2019年9月1日在西藏山南市妇幼保健院产科出生的足月新生儿的出生体质量、母亲基本信息及孕期合并症等资料,按照胎龄和性别分组,与平原地区相应数据比较,并分析其中发生LBWI的危险因素。结果:共纳入716例藏族足月新生儿,各胎龄平均体质量均低于全国相应胎龄的平均体质量,差异具有统计学意义(P<0.05)。LBWI共94例(占13.13%),卡方检验示出生季节、产妇年龄、产妇贫血、妊娠高血压综合征(妊高征)、肝功能受损、妊娠期糖尿病、羊水过少、初中以下文化水平、居住地海拔更高(≥4 000 m)及出生窒息等10个因素与LBWI有关(P<0.05),多因素Logistic回归分析示冬春季出生、产妇年龄≥35岁、产妇贫血、妊高征、羊水过少以及出生窒息是LBWI的危险因素,初中及以上文化水平、居住地海拔<4 000 m是LBWI的保护因素。结论:高海拔地区足月新生儿的平均出生体质量低于平原地区同胎龄新生儿。根据该地区发生LBWI的主要危险因素,建议可通过加强高海拔地区孕产妇孕期营养指导和宣教,规律产检管理以及配合政府由高海拔向低海拔地区移民等方式,降低LBWI发生率,提高该地区人口出生质量。

关键词：超高海拔足月新生儿出生体质量影响因素