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Emodin enhances alveolar epithelial barrier function in rats with experimental acute pancreatitis

World Journal of Gastroenterology 2010年第24期16卷 2994-3001页

作者： xia, xian-ming Wang, Fang-Yu Wang, Zhen-Kai Wan, Hai-Jun Xu, Wen-An Lu, Heng Jinling Hosp Dept Gastroenterol & Hepatol Nanjing 210002 Jiangsu Prov Peoples R China

AIM: To investigate the effect of emodin on expression of claudin4, claudin5 and occludin, as well as the alveolar epithelial barrier in rats with pancreatitis induced by sodium taurocholate. METHODS: Experimental pancreatitis was induced by retrograde injection of 5% sodium taurocholate into the biliopancreatic duct. Emodin was injected via the external jugular vein 3 h after induction of acute pancreatitis. Rats from sham operation group and acute pancreatitis group were injected with normal saline (an equivalent volume as emodin) at the same time point. Samples of lung and serum were obtained 6 h after drug administration. Pulmonary morphology was examined with HE staining. Pulmonary edema was estimated by measuring water content in lung tissue samples. Tumor necrosis factorα (TNFα) and interleukin6 (IL6) level were measured by enzymelinked immunospecific assay. Serum amylase and pulmonary myeloperoxidase (MPO) activity were detected by spectrophotometry. Alveolar epithelial barrier was assessed by pulmonary dye extravasation. Expression of claudin4, claudin5 and occludin in lung tissue samples was examined by immunohistology, quantitative realtime reverse transcription polymerase chain reaction and Western blotting analysis, ***: Pancreatitis-associated lung injury was char-acterized by pulmonary edema, leukocyte infiltration, alveolar collapse, and elevated serum amylase level. The pulmonary damage, pulmonary pathological scores, serum amylase and MPO activity, TNF-α and IL-6 levels, and wet/dry ratio were decreased in rats after treatment with emodin. Immunostaining of claudin-4, claudin-5 and occludin was detected in lung tissue samples from rats in sham operation group, which was distributed in alveolar epithelium, vascular endothelium, and bron-chial epithelium, respectively. The mRNA and protein expression levels of claudin-4, claudin-5 and occludin in lung tissue samples were markedly decreased, the expression level of claudin-4, claudin-

关键词： Acute pancreatitis Emodin Lung injury Claudin Occludin Epithelial barrier

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Emodin and baicalein inhibit pancreatic stromal derived factor-1 expression in rats with acute pancreatitis

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Hepatobiliary & Pancreatic Diseases International 2009年第2期8卷 201-208页

作者： Li, Zong-Fang xia, xian-ming Huang, Chen Zhang, Shu Zhang, Jian Zhang, Ai-Jun Xi An Jiao Tong Univ Sch Med Affiliated Hosp 2 Dept Surg Xian 710004 Peoples R China Xi An Jiao Tong Univ Minist Educ Key Lab Environm & Genes Related Dis Xian 710004 Peoples R China Wannan Med Coll Yijishan Hosp Dept Surg Wuhu 241001 Peoples R China

BACKGROUND: Stromal derived factor-1 (SDF-1) is an efficacious leukocyte chemoattractant, which can attract lymphocytes and mononuclear cells from bloodstream into the site of inflammation. Emodin., an anthraquinone derivative from Radix et Rhizoma Rhei, and baicalein, a flavone from Scutellaria baicalensis Georgi, both have been reported to possess anti-inflammatory activities. The expression pattern of SDF-1 in experimental acute pancreatitis (AP) and the effect of emodin or baicalein on that are not well defined. The present study aimed to investigate the effects of emodin and baicalein on pancreatic myeloperoxidase (MPO) activity (reflecting leukocyte sequestration) and cytokine production, as well as tissue SDF-1 expression in the setting of AP. METHODS: A :rat model of AP was induced by administration (of 5% sodium taurocholate through the biliopancreatic duct. The level of tumor necrosis factor-a (TNF-alpha), interleukin-6 (IL-6) and MPO in the pancreas, and serum amylase were tested by immunohistochemistry, ELISA and chromatometry. The expressions of SDF-1 alpha and SDF-1 beta were detected by real-time PCR, Western blotting, and immunohistochemistry. RESULT: Combination of emodin and baicalein significantly reduced pancreatic TNIP-alpha, IL-6 and MPO, and also inhibited pancreatic SDF-1 expression. CONCLUSIONS: The inhibition of SDF-1 expression by emodin and baicalein might contribute, in part at least, to the amelioration of pancreatic inflammation. The present study also shows benefits of simultaneous treatment of AP.

关键词： acute pancreatitis stromal derived factor-1 myeloperoxidase traditional Chinese medicine

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CXCR4 antagonist AMD3100 attenuates colonic damage in mice with experimental colitis

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World Journal of Gastroenterology 2010年第23期16卷 2873-2880页

作者： xia, xian-ming Wang, Fang-Yu Xu, Wen-An Wang, Zhen-Kai Liu, Jiong Lu, You-Ke Jin, Xin-Xin Lu, Heng Shen, Yun-Zhu Jinling Hosp Dept Gastroenterol & Hepatol Nanjing 210002 Jiangsu Prov Peoples R China

AIM:To investigate the effects of the chemokine stromal cell-derived factor-1(CXCL12) receptor(CXCR4) antagonist AMD3100 on colonic inflammation and epithelial barrier in dextran sulfate sodium(DSS)-induced colitis in ***:Experimental colitis was induced by administration of 5% DSS for 7 d,and assays performed on intestinal segments from the ileocecal valve to the *** morphology was examined by hematoxylin and eosin *** cytokines were determined by enzyme-linked immunosorbent ***(MPO) activity(indicator of inflammatory infiltration) was observed *** permeability was assessed by mucosal-to-serosal clearance of fluorescein isothiocyanate-conjugated dextran 4000(FD4) in everted gut *** apoptosis of colonic epithelium was assessed by Hoechst-33342 *** further elucidate the role of CXCR4 in colonic inflammation,we also investigated the effect of AMD3100 on migration and cytokine production of isolated peripheral blood mononuclear cells(PBMCs).RESULTS:DSS-induced colitis was characterized by morphologic changes,as well as increased colonic cytokines,inflammatory infiltration,epithelial apoptosis,and intestinal permeability in *** AMD3100-treated mice,epithelial destruction,inflammatory infiltration,and submucosal edema were markedly reduced;colonic tumor necrosis factor-α(TNF-α),interleukin-6(IL-6) and interferon-γ(IFN-γ) levels,as well as MPO activity were significantly *** intestinal permeability in DSS-treated mice was signif icantly reduced by *** number of apoptotic cells in colitis mice was markedly increased after DSS administration,and decreased when treated with the CXCR4 antagonist *** pre-activated PBMCs,CXCL12 stimulation signif icantly increased the migration of PBMCs,and was inhibited by *** increased TNF-α,IL-6,and IFN-γ from CXCL12-treated PBMCs were also reduced by ***:The CXCR4 antagonist AMD3100 exerts the

关键词： Ulcerative colitis Chemokine stromal cellderived factor-1 receptor Inflammation Apoptosis Intestinal permeability Epithelial barrier

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Rabeprazole 10 mg versus 20 mg in preventing relapse of gastroesophageal reflux disease： a meta-analysis

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Chinese Medical Journal 2013年第16期126卷 3146-3150页

作者： ZHU Hai-di WANG Heng xia xian-ming XU Shu-man LAN Yao Huazhong University of Science and Technology Wuhan Hubei430030 China The Fourth Affiliated Hospital of Anhui Medical University HefeiAnhui 230022 China Social Science and Health Management School Anhui MedicalUniversity Hefei Anhui 230032 China

Background Several randomized controlled trials （RCTs） have compared endoscopic and symptomatic relapses in patients with erosive gastroesophageal reflux disease （GERD）.We have summarized current evidence for rabeprazole 10 or 20 mg once daily for GERD maintenance treatment over 1 or 5 *** MEDLINE,EMBASE,and the Cochrane Central Register of Controlled Trials were searched,through August 2012,for eligible RCTs of adults with erosive *** efficacies of rabeprazole 10 and 20 mg/d were *** The search identified 288 citations,and five RCTs containing 1480 patients were considered *** relapse rates did not differ significantly between patients treated with rabeprazole 10 and 20 mg/d for 1 year （relative risk （RR）=1.29; 95％ confidence interval （CI）：0.97-1.72）,but differed in patients treated for 5 years （RR=1.274; 95％ CI：1.005-1.615）.Endoscopic relapse rates differed significantly between rabeprazole 10 and 20 mg/d for 1 year （RR=1.92;95％ CI：1.21-3.06）,for 5 years （RR=1.667; 95％ CI：1.073-2.589）,and in combined 1-and 5-year maintenance trials （RR=1.785; 95％ CI：1.298-2.456）.Conclusion Rabeprazole 20 mg/d was superior to rabeprazole 10 mg/d in preventing endoscopic relapse of erosive GERD,but that the two dosages were equivalent in symptomatic relief over 1 year.

关键词： gastroesophageal reflux disease proton pump inhibitors rabeprazole relapse endoscopy heartburn

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无人直升机飞控系统设计与应用

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南京航空航天大学学报 2005年第4期37卷 476-478页

作者：顾冬雷夏先明高正南京航空航天大学直升机旋翼动力学国家重点实验室南京210016 南京模拟技术研究所南京210016

论述了将Voyage模型直升机改造成全自主无人直升机的工作。首先,改造了直升机结构,设计了飞控电子设备和基于客户/服务模式的飞控软件和地面站软件;其次,利用扫频数据进行频域辨识,建立了直升机姿态通道的动力学模型;最后,设计了飞行控... 详细信息

论述了将Voyage模型直升机改造成全自主无人直升机的工作。首先,改造了直升机结构,设计了飞控电子设备和基于客户/服务模式的飞控软件和地面站软件;其次,利用扫频数据进行频域辨识,建立了直升机姿态通道的动力学模型;最后,设计了飞行控制律,实现了在悬停和低速下的全自主飞行。

关键词：无人直升机电子设备频域辨识飞行控制

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Morphological changes of blood spleen barrier in portal hypertensive spleen

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Chinese Medical Journal 2008年第6期121卷 561-565页

作者： LI Zong-fang ZHANG Shu HUANG Ying xia xian-ming LI Ai-min PAN Dun ZHANG Wei WANG Juan Department of General Surgery Second Affiliated Hospital School of Medicine Xi'anJiaotong University Xi'an Shaanxi 710004 China Department of Pathology Second Affiliated Hospital School of Medicine Xi'anJiaotong University Xi'an Shaanxi 710004 China

The pathogenesis of hypersplenism and the immune function of the spleen in patients with portal hypertension （PH） remain obscure. This study aimed to evaluate the morphological changes of blood spleen barrier in spleen with hypersplenism due to PH and provide evidence for an in-depth investigation of the immune function of the spleen with hypersplenism and the mechanism of hypersplenism. Methods Spleen samples from 12 portal hypertensive patients and 4 patients with traumatic ruptures of spleen were examined. The samples of spleen were made into pathological sections, stained with Masson trichrome stain, Gomori stain, and CD68, CD34 immunohistochemistry, and were examined microscopically for the changes in the distribution of collagen fibers, reticular fibers, macrophages, and vascular endothelial cells. The changes in ultrastructure of macrophages and endothelial cells in marginal zone were also evaluated by transmission electron microscopy. Results As compared to the normal spleen, the density of macrophage in the PH spleen was decreased, but the macrophages were mainly located in the marginal zone and distributed around the splenic corpuscle, with many villi and pseudopodium-like protrusion on the cell surface. The accrementition of collagen fibers was obvious around the splenic corpuscle and central artery. The increased reticulate fibers encircled the splenic corpuscle with more connection between the fibers. The vascular endothelial cells were in diffused distribution, without any regionality in PH spleen, but the vessel with enlarged lumina increased in red pulp. Conclusions The morphological changes of the blood spleen barrier can be one of the pathological fundaments for the abnormality of the immune function and the increased destruction of blood cells located in the spleens of patients with PH However. this still entails clarification.

关键词： hypertension, portal spleen blood spleen barrier hypersplenism

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往复挤压对挤压态ZK60镁合金微观组织和力学性能的影响

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塑性工程学报 2017年第3期24卷 78-83页

作者：夏显明谢瑞薛克敏李萍安徽科技学院机械工程学院安徽凤阳233100 合肥工业大学材料科学与工程学院安徽合肥230009

在350℃下,对挤压态ZK60镁合金分别进行1、4、8道次的往复挤压变形(CEC)。利用金相显微镜(OM)、透射电镜(TEM)观察往复挤压前后ZK60镁合金的微观组织,利用X射线衍射仪(XRD)分析变形前后晶面取向变化,在万能拉伸试验机上测试变形前后镁... 详细信息

在350℃下,对挤压态ZK60镁合金分别进行1、4、8道次的往复挤压变形(CEC)。利用金相显微镜(OM)、透射电镜(TEM)观察往复挤压前后ZK60镁合金的微观组织,利用X射线衍射仪(XRD)分析变形前后晶面取向变化,在万能拉伸试验机上测试变形前后镁合金的力学性能,并利用扫描电镜(SEM)观察拉伸断口形貌。往复挤压后的检测结果表明,挤压态ZK60镁合金晶粒显著细化,晶粒尺寸分布较均匀,随着挤压道次增多,晶粒尺寸逐渐减小;1道次变形后组织内产生了大量晶格缺陷,出现了大角度晶界,第二相粒子分布在晶粒内部和晶界上;各晶面衍射峰增强,拉伸断口内存在大量基体撕裂棱和明显的韧窝分布;ZK60镁合金的力学性能变化较大,随着挤压道次增多,伸长率大幅提高,抗拉强度小幅增大,而屈服强度降低。

关键词： ZK60镁合金往复挤压微观组织力学性能

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运用电教媒体优化语文课堂教学

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芜湖职业技术学院学报 2001年第1期3卷 72-72,69页

作者：夏明娴淮南一中安徽淮南233000

现在许多学生对语文学习没有兴趣,厌倦上语文课。究其原因,主要是因为现在的语文教学形式单一,教法陈旧。那么如何改进语文课堂教学,激发学生学语文的兴趣呢?利用现代化教学手段即电教媒体可以优化语文教学,提高课堂教学效率。

关键词：电教媒体课堂教学语文教学教学形式教学方法教学手段教学效率

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ZK60镁合金往复挤压实验研究

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塑性工程学报 2017年第2期24卷 105-110页

作者：夏显明薛克敏李萍魏宝丽安徽科技学院机械工程学院安徽凤阳233100 合肥工业大学材料科学与工程学院安徽合肥230009

通过塑性变形装置实现了挤压态ZK60镁合金往复挤压实验,探讨了显微组织演变过程和力学性能变化。结果表明:相比初始挤压态,350℃往复挤压后各个道次的试样具有更加优良的显微组织和力学性能。随挤压道次增加,显微组织晶粒更加细化,等轴... 详细信息

通过塑性变形装置实现了挤压态ZK60镁合金往复挤压实验,探讨了显微组织演变过程和力学性能变化。结果表明:相比初始挤压态,350℃往复挤压后各个道次的试样具有更加优良的显微组织和力学性能。随挤压道次增加,显微组织晶粒更加细化,等轴细小晶粒增多,组织均匀性不断提高;拉伸断口形貌显示随着道次增加,韧窝数量与深度明显增加,变形能力提高显著。拉伸实验数据表明,往复挤压很大程度上改善了ZK60镁合金的力学性能,特别是塑性变形能力。1道次往复挤压后,径向硬度都比原始态高,并随温度升高有下降的趋势,轴向硬度也随着温度升高而降低,390℃下试样轴向硬度与初始样硬度值接近;350℃下不同道次往复挤压后,试样中部径向、轴向硬度随道次增大而降低,而颈部径向硬度呈不规律性变化。

关键词： ZK60镁合金往复挤压晶粒细化组织与性能

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ZK60镁合金高压扭转模拟及实验研究

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塑性工程学报 2017年第4期24卷 54-61页

作者：夏显明魏宝丽薛克敏李萍安徽科技学院机械工程学院安徽凤阳233100 合肥工业大学材料科学与工程学院安徽合肥230009

为了研究高压扭转工艺对ZK60镁合金组织及性能的影响,采用有限元法对ZK60镁合金试样高压扭转成形过程进行数值模拟,分析成形过程中等效应力、应变分布及变化趋势。通过压扭设备和专用模具对镁合金试样进行高压扭转实验,采用金相显微镜... 详细信息

为了研究高压扭转工艺对ZK60镁合金组织及性能的影响,采用有限元法对ZK60镁合金试样高压扭转成形过程进行数值模拟,分析成形过程中等效应力、应变分布及变化趋势。通过压扭设备和专用模具对镁合金试样进行高压扭转实验,采用金相显微镜观察试样的显微组织,利用维氏硬度计测量试样的显微硬度。结果表明:高压扭转能有效细化晶粒,改善材料组织结构;在一定的压力下,随着压扭圈数增加,晶粒得到细化,整体显微硬度有较大的提高;高压扭转后试样的显微硬度沿试样径向呈V型分布,与有限元模拟中试样径向的等效应变分布趋势一致。试样不同区域的等效应力、应变和显微硬度差异较大,边缘处的等效应力、应变最大,显微维氏硬度值最高。

关键词： ZK60镁合金高压扭转有限元模拟显微硬度显微组织

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