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中药癌痛克对人肝癌细胞HepG2增殖、凋亡及Rb基因表达的影响

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中国组织工程研究与临床康复 2007年第12期11卷 2253-2256页

作者：徐波朱光辉夏金堂李书华广州市第一人民医院普外科广东省广州市510180 广州医学院病理教研室广东省广州市510180

目的:通过观察不同浓度癌痛克对肝癌细胞株HepG2增殖及凋亡的作用,以及在相应状态下细胞内Rb基因表达量的改变,探讨中药癌痛克抗肝癌的可能作用机制。方法:实验于2005-09/2006-03在广州医学院中心实验室完成。取对数生长期的HepG2细胞,... 详细信息

目的:通过观察不同浓度癌痛克对肝癌细胞株HepG2增殖及凋亡的作用,以及在相应状态下细胞内Rb基因表达量的改变,探讨中药癌痛克抗肝癌的可能作用机制。方法:实验于2005-09/2006-03在广州医学院中心实验室完成。取对数生长期的HepG2细胞,使细胞静止于G0/G1期。随机分为癌痛克2,10,50mg/L组和对照组,癌痛克2,10,50mg/L组分别加入对应浓度癌痛克(购自河南省肿瘤研究所,由金蝎、土元、九香虫、大黄、人参、灵芝、黄芪等纯中药组成的粉状制剂,功能:消癌肿、消癌痛、消积水、升白排毒),对照组不加药物,每组设4个复孔。MTT法检测各组细胞24,48,72h增殖率,细胞增殖率=[(A570癌痛克组-A570对照组)/A570对照组]×100%;以流式细胞术检测各组细胞24,48,72h凋亡率;RT-PCR方法检测各组细胞24,48,72h细胞内Rb基因mRNA表达量。结果:①2～50mg/L癌痛克具有明显的增殖抑制作用,癌痛克2,10,50mg/L组在24,48,72h与对照组比较,差异有显著性意义(P0.05);3组各时点比较差异有显著性意义(P<0.05)。结论:中药癌痛克可通过抑制HepG2细胞增殖或诱导其凋亡,而发挥抗肝癌作用;且其诱导细胞凋亡的机制之一可能为通过增加Rb基因的表达而实现。

关键词：癌肝细胞/病理学基因.肿瘤抑制基因视网膜母细胞瘤/遗传学抗肿瘤药/药理学细胞凋亡/药物作用

携带肿瘤坏死因子相关细胞凋亡诱导配体重组腺相关病毒的构建及鉴定

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中国组织工程研究与临床康复 2007年第10期11卷 1966-1968,1971页

作者：夏金堂徐波李雯王劭晟赖越元伍兆锋李书华广州市第一人民医院普外科广东省广州市510180 中山大学附属第一医院普通外科实验室广东省广州市510080

目的:构建一种携带肿瘤坏死因子相关细胞凋亡诱导配体(tumor necrosis factor related apoptosis inducing ligand,TRAIL)基因的重组腺相关病毒(recombined adeo-associated virus,rAAV)载体。方法:实验于2005-09/2006-04在中山大学附属第一医院普通外科实验室完成。首先构建携带可溶性肿瘤坏死因子相关细胞凋亡诱导配体基因的穿梭质粒pAAV-sTRAIL,利用磷酸钙共沉淀法将穿梭质粒共转染入HEK293细胞中,采用细胞内质粒DNA同源重组法构建重组腺相关病毒rAAV-sTRAIL。以噬斑分析法筛选单克隆重组腺相关病毒;聚合酶链反应法鉴定阳性重组腺相关病毒;氯化铯密度梯度离心法纯化病毒;紫外分光光度仪测定病毒颗粒数及纯度,噬斑分析法测定病毒感染滴度;Western blot检测rAAV-sTRAIL在293细胞中的表达情况。结果:成功构建了重组腺相关病毒载体rAAV-sTRAIL,制备的病毒纯度好、滴度高,且在293转导细胞中能有效表达目的基因sTRAIL。结论:构建的重组腺相关病毒载体rAAV-sTRAIL,为组织工程相关细胞转基因构建及临床应用提供先进的载体系统。

关键词：肿瘤坏死因子相关细胞凋亡诱导配体腺相关病毒基因治疗

Results and Future Plan of jt-60U towards Steady-State Tokamak Reactor

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Plasma Science and Technology 2004年第1期6卷 2151-2158页

作者： S.Sakurai the jt-60 Team Japan Atomic Energy Research Institute Naka Ibaraki 311-0193 JapanJapan Atomic Energy Research Institute Naka Ibaraki 311-0193 Japan

Recent results of jt-60U towards establishment of physics basis for ITER andadvanced tokamak operation are presented. Progress in high integrated performance is achieved withimprovement of N-NB and ECRF heating systems. In the next experimental campaign 2003～2004,discharge duration with 17 MW heating will be extended up to 30s for sustaining high-beta plasmalonger than the current diffusion time. Superconducting modification of jt-60 is planned todemonstrate high-beta plasma sustainment exceeding ideal MHD instability limit without wallstabilization.

关键词： jt-60U confinement study steady-state operation high confinement plasma plan and future plan

Present Status of jt-60SA Project and Development of Heating Systems for jt-60SA

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Plasma Science and Technology 2011年第3期13卷 367-375页

作者： Yoshitaka IKEDA jt-60SA Team Japan Atomic Energy Agency 801-1 Mukoyama Naka Ibaraki 311-0193 Japan

Present status of the jt-60SA （jt-60 Super Advanced） project, implemented jointly by Europe and Japan since 2007, is described. The design of the main tokamak components was completed in late 2008, and all the scientific missions are preserved to contribute to ITER and DEMO reactors. The construction of the jt-60SA has begun with procurement activities for the superconducting magnet systems, vacuum vessel, in-vessel components and other components under the relevant procurement arrangements between the implementing agencies of JAEA （Japan Atomic Energy Agency） in Japan and Fusion for Energy in Europe. Designs and developments of the auxiliary heating systems for jt-60SA have been progressing at JAEA so as to provide the total injection power of 41 MW for 100 s.

关键词： jt-60SA ITER DEMO JAEA heating systems

Influence of Hot Isostatic Pressing on Microstructuresand Properties of NiAl/Cr(Mo)-TiC in-situMultiphase Composites

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Journal of Materials Science & Technology 1998年第4期14卷 331-334页

作者： Jiang, DT Guo, jt Chinese Acad Sci Inst Met Res Shenyang 110015 Peoples R China

A novel in-situ multiphase NiAl-based composite was investigated on its microstructures and properties influenced by HIP treatment. In the as- processed composites, a fine array of NiAl precipitates was found to be distributed in Cr(Mo) phase. TiC particles tended to segregate at grain or phase boundaries. In addition, a number of dislocations were observed to exist in NiAl grains meanwhile fewer in Cr(Mo) grains. Little change in the morphology was found except that nearly fully densification was achieved. Some changes in microstructures were notable including coarsening of NiAl precipitates in Cr(Mo) phase and increase in dislocation density in NiAl phase. After HIP, the strength of the composites increased significantly compared with the as-processed composites. The reason for the enhanced strength of the composites is also discussed.

关键词： Metallic matrix composites

Influence of oscillator phase on oscillation frequency and sensitivity of an electrode-separated piezoelectric sensor

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Chinese Journal of Chemistry 1996年第3期14卷 235-243页

作者： Shen, DZ Kang, Q Hu, jt SHANDONG UNIV CHEM COLLJINAN 250100SHANDONGPEOPLES R CHINA

Theoretical consideration was proposed for the influences of the oscillator phase on the response nature of an electrode-separated piezoelectric sensor (ESPS) to liquid properties and verified experimentally. For the oscillators with different phases, the oscillation frequency of the ESPS may increase, maintain nearly constant or decrease with increasing conductivity in low conductivity solutions. The sensitivity of response to density and viscosity increases slightly with increasing oscillator phase. The response to permittivity depends hardly on the oscillator phase. In addition, the dependence of the oscillation frequency of the ESPS on the supply voltage of the oscillator was explained. The ESPS was used to determine atropine sulfate with a detection limit of 1.6x10(-7) mol/L.

关键词： piezoelectric sensor oscillator phase oscillation frequency liquid properties

Synthesis and Luminescence Properties of Barium Fluorotungstate Phosphors

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Chinese Chemical Letters 1997年第7期8卷 643-644页

作者： Yang, SJ Sun, jt Qin, ZB HUBEI NORMAL UNIV DEPT CHEMHUNGSHI 435002PEOPLES R CHINA WUHAN UNIV DEPT CHEMWUHAN 430072PEOPLES R CHINA

The investigation of the system BaF2-WO3 revealed the existence of a fluoro-tungstate formulated as Ba2WO3F4. The Ba2WO3F4 was prepared with the solid state reaction method. Under UV or X-ray excitation, Ba2WO3F4 exhibits a very strong blue-green emission peaking at 480 nm at room temperature. The luminescence properties are similar to those of the tungstate, especially MgWO4. The luminescence and energy transfer of tungstates are well known for a long time and was reviewed by Blasse([1]). But barium tungstate show only weak luminescence even down to liquid helium temperature([2]). We were surprised to find that Ba2WO3F4 is an efficient phosphor which can be practically used. In this paper, the preparation method and luminescence properties of Ba2WO3F4 phosphor were investigated. The relationship between the luminescent properties and crystal structure was discussed.

关键词： Ba nm

DIFFUSION WELDING FOR 6061 Al REINFORCED WITH SiCw WHISKER

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Journal of Wuhan University of Technology(Materials Science) 1999年第1期14卷 1-5页

作者： Niu, jt Liu, LM Han, LH Harbin Inst Technol Sch Mat Sci & Engn Harbin 150001 Peoples R China

The weldability of SiCw/6061Al -was investigated. From the results of experiments, it is found that the strength of joint is mainly influenced by welding temperature, and high strength of joint is obtained under melding temperature between liquidus and solidus of matrix alloy. Diffusion welding of SiCw/6061Al was successfully realized with appropriate ivelding parameters.

关键词： SiCw/6061Al composite diffusion welding welding temperature

Powder Microelectrodes Applied to NO_2 Detection

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Chinese Chemical Letters 1999年第1期10卷 47-50页

作者： Yan, JW Lu, jt Liu, PF Wuhan Univ Dept Chem Wuhan 430072 Peoples R China

The reduction of nitrite at Au or carbon electrode in-H_2SO_4 was found to follow achemical-electrochemical (CE) mechanism with a very thin (4×10(-8)cm) preceding reaction *** was proposed and experimentally verified that for such kind of electrode processes the totalreaction the could be effectively enhanced by using electrodes with increased true surface *** as porous electrodes. As a combination of porous electrode and microelectrode, the powdermicroclectrode shows excellent performance for nitrite detection.

关键词： Nitrite reduction microelectrode CE mechanism