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EFFECT OF STRESS-INDUCED REACTIONS ON MORPHOLOGICAL STRUCTURE AND PROCESSABILITY OF PVC DURING PAN-MILLING

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Chinese Journal of Polymer Science 2005年第2期23卷 227-234页

作者： Hua-weiZou WenXu Qing-fenFeng xixu TheStateKeyLaboratoryofPolymerMaterialsEngineering PolymerResearchInstituteSichuanUniversityChengdu610065China

The effect of pan-milling on morphological structure,processability and properties of PVC was studied throughSEM,FTIR,granulometer,GPC and mechanical properties test in the hope of gaining ease in operation,needless ofplasticizers,a clean and efficient route for improving the processability of PVC through stress-induced reactions,fulfilling the idea of“plasticizing PVC by itself”.The experimental results show that during pan-milling at ambienttemperature,within 2-3 min,the microcrystalline structure of PVC becomes indistinct,the grain size of PVC is reducedfrom 130-160 μm to 1-50 μm the molecular weight of PVC is slightly decreased,the variation of molecular weightdistribution is indistinct,the plasticizing time and torque at balance drop a great deal from 71-132 s to 31-33 s and from18.2-22.1 Nm to 14.7-18.4 Nm,respectively,the processability of PVC is markedly improved,and the mechanicalproperties get enhanced too.

关键词： Poly(vinyl chloride) Stress-induced reaction Morphological structure Processability.

苦荞FtWRKY28基因克隆及其在低磷与激素诱导下的表达分析

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西北植物学报 2024年第6期44卷 930-937页

作者：文碧瑶彭喜旭田建红邬清韬王海华唐新科湖南科技大学生命科学与健康学院湖南湘潭411201 经济作物遗传改良与综合利用湖南省重点实验室湖南湘潭411201 重金属污染土壤生态修复与安全利用湖南省高校重点实验室湖南湘潭411201

[目的]WRKY转录因子参与植物对低磷胁迫反应的调控。基于前期苦荞在低磷胁迫下的转录组数据,克隆FtWRKY28基因,预测基因及其编码蛋白的序列结构特征,分析基因在各器官中以及在低磷和激素处理下的表达模式、蛋白的亚细胞定位与转录激活活... 详细信息

[目的]WRKY转录因子参与植物对低磷胁迫反应的调控。基于前期苦荞在低磷胁迫下的转录组数据,克隆FtWRKY28基因,预测基因及其编码蛋白的序列结构特征,分析基因在各器官中以及在低磷和激素处理下的表达模式、蛋白的亚细胞定位与转录激活活性,为阐明基因的生物学功能奠定基础。[方法]基于苦荞基因组数据库注释设计特异引物,用逆转录PCR从低磷处理的苦荞根RNA样中克隆FtWRKY28的完整编码序列,用生物信息学方法分析基因与蛋白的结构以及同源蛋白的进化关系,用实时荧光定量分析基因的表达模式,用拟南芥原生质体瞬时表达体系分析蛋白的亚细胞定位,用酵母单杂交分析蛋白的转录激活活性。[结果]FtWRKY28的完整编码序列长876bp,编码1个含291个氨基酸、有1个WRKY结构域、锌指结构域为C2H2型的蛋白,归属WRKY家族Ⅱ组。FtWRKY28绝大部分定位于细胞核,具有转录激活活性。FtWRKY28在根中表达水平最高,受低磷、吲哚乙酸、赤霉素3和6-苄氨基嘌呤显著诱导。[结论]FtWRKY28具有转录因子的基本结构与生物化学特征,可能通过生长素、赤霉素、细胞分裂素信号网络的交互作用,调控苦荞在低磷胁迫下的响应过程。

关键词：苦荞低磷胁迫 WRKY基因基因表达结构预测生化特征

Asiatic acid inhibits lung cancer cell growth in vitro and in vivo by destroying mitochondria

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药学学报：英文版 2017年第1期7卷 65-72页

作者： Tiancong Wu Ji Geng Wenjie Guo Jing Gao xixu Zhu Department of Radiation Oncology Jinling HospitalMedical School of Nanjing University School of Pharmacy Jiangsu University School of Life Science Nanjing University

Asiatic acid(AA), a pentacyclic triterpene found in Centella asiatica, displays significant antiproliferative effects on cancer cells in vitro although the underlying mechanism of this effect remains unknown. This study investigated the efficacy and mechanism of action of AA against lung cancer both in vivo and in vitro. Using the MTT assay, AA was found to induce apoptosis in a dose- and timedependent manner, an effect enhanced by pretreatment with an autophagy inhibitor. It also elevated expression of microtubule-associated protein 1 light chain 3(LC3) and decreased the expression of ***, exposure to AA resulted in collapse of the mitochondrial membrane potential and generation of reactive oxygen species(ROS), suggesting mitochondria are the target of AA. In the mouse lung cancer xenograft model, oral administration of AA significantly inhibited tumor volume and weight accompanied by significant apoptosis of lung cancer cells. In addition, it led to a significant decrease in the expression of proliferating cell nuclear antigen(PCNA). In summary, the results show that AA significantly reduces lung cancer cell growth both in vitro and in vivo and that the associated apoptosis is mediated through mitochondrial damage.

关键词： Asiatic acid Lung cancer Apoptosis Mitochondria Reactive oxygen species

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思维导图在高中生物学教学中的应用与拓展

生物学通报 2024年第4期59卷 13-17页

作者：唐玉琴李莉龚西旭贵州师范大学生命科学学院贵州贵阳550025 贵州师范大学附属中学贵州贵阳550025

思维导图因图文结合的可视化优势在教学中应用广泛,尤其适用于内容覆盖面广和层次多样的生物学课程教学。思维导图作为教学常用的工具之一,具有激发思维、整理知识、改善记忆等优势。通过文献分析工具SATI3.2,从思维导图在不同学段、学... 详细信息

思维导图因图文结合的可视化优势在教学中应用广泛,尤其适用于内容覆盖面广和层次多样的生物学课程教学。思维导图作为教学常用的工具之一,具有激发思维、整理知识、改善记忆等优势。通过文献分析工具SATI3.2,从思维导图在不同学段、学科中的应用及对高中生物学学科核心素养的支撑等方面,综述了在高中生物学教学中的适用性和普遍性,并结合高中生物学学科核心素养的基本要求,综合分析思维导图在高中生物学教学中的重要功能和拓展需求。

关键词：思维导图生物学教学高中生物学核心素养科学思维

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高校精品在线开放课程教学资源的整合探研

成才之路 2024年第26期 85-88页

作者：曾锦吴天钰严颖陈熙栩昭通卫生职业学院云南昭通657000

迅速发展的信息技术为各行各业注入了新的活力,教育领域亦然。精品在线开放课程作为依托于信息技术形成的重要教学资源,被越来越多的人认可、接受。为此,教育工作者需加大力度,全面整合在线开放课程教学资源,使其实现精品化,从而为学生... 详细信息

迅速发展的信息技术为各行各业注入了新的活力,教育领域亦然。精品在线开放课程作为依托于信息技术形成的重要教学资源,被越来越多的人认可、接受。为此,教育工作者需加大力度,全面整合在线开放课程教学资源,使其实现精品化,从而为学生提供更优质的教育服务。但目前,在线开放课程的整合工作尚存在不足之处,教育工作者可基于教学资源整合原则,做好课程整体规划工作,积极提升课程利用率,并及时跟进课程体系的后续更新工作,以精品在线开放课程的合理有效整合,助推高校精品在线开放课程高质量发展。

关键词：在线开放课程教学资源服务社会特色规划利用率更新问题

维普期刊数据库博看期刊

Differential Expression of Rice Valine-Qlutamine Gene Family in Response to Nitric Oxide and Regulatory Circuit of OsVQ7 and OsWRKY24

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Rice science 2020年第1期27卷 10-20页

作者： PENG xixu XIAO Ting MENG Jiao TAO Zong ZHOU Dinggang TANG Xinke WANG Haihua School of Life Science Hunan University of Science and TechnologyXiangtan 411201China Key Laboratory of Integrated Management of the Pests and Diseases on Horticultural Crops in Hunan Province Xiangtan 411201China Key Laboratory of Ecological Remediation and Safe Utilization of Heavy Metal-Polluted Soils College of Hunan ProvinceXiangtan 411201China

The functional diversity of plant valine-qlutamine(VQ) proteins is closely associated with their partners WRKY transcription factors, and also with a complex network of signaling pathways that mediated by hormone molecules. We reported genome-wide expression profiles of differentially expressed rice VQ genes under nitric oxide(NO) treatment based on a microarray analysis. Cluster analysis of expression patterns revealed that some VQ genes and WRKY genes shared similar expression trends. Prediction of cis-elements showed that W-box or W-box-like sequences were overrepresented within the promoters of most of NO-responsive VQ genes. In particular, the similarly expressed Os VQ7 and Os WRKY24 showed great induction upon NO triggering. Transient expression assay and chromatin immunoprecipitation analysis demonstrated that OsWRKY24 was specifically bound to the promoter regions of Os VQ7 and Os WRKY24 itself, which contain multiple copies of W-box or W-box-like cis-elements. Yeast-two-hybrid assay indicated that OsWRKY24 can interact physically with OsVQ7 through the C-terminal of WRKY domain. The results suggested that OsVQ7 and OsWRKY24 may form an auto-and cross-regulation circuit that is required for tight regulation and fine-tuning of physiological processes they are involved in. These findings provided a solid foundation for exploring the specific functions of the VQ protein family in NO signaling pathway.

关键词： rice valine-qlutamine protein expression profile WRKY transcription factor nitric oxide regulatory circuit

Identification and fine mapping of a fertility restorer gene for wild abortive cytoplasmic male sterility in the elite indica rice non-restorer line 9311

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The Crop Journal 2023年第3期11卷 887-894页

作者： Zuopeng Xu Yuanyue Du xixu Li Ruixuan Wang Zhi'ai Chen Xiangqiang Zhao Qiaoquan Liu Shuzhu Tang Honggen Zhang Jiangsu Key Laboratory of Crop Genetics and Physiology/Key Laboratory of Plant Functional Genomics of the Ministry of Education/Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding Agricultural CollegeYangzhou UniversityYangzhou 225009JiangsuChina Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops Yangzhou UniversityYangzhou 225009JiangsuChina School of Life Sciences Nantong UniversityNantong 226019JiangsuChina

The wild abortive(WA)-type cytoplasmic male sterility(CMS)derived from the wild rice species Oryza rufipogon *** used widely in three-line indica *** identification and mapping of restorer of fertility(Rf)genes aided in the development of WA-type *** we report that testcross F1 plants from the WA-type CMS line and 9311 exhibited stainable pollen grains with no seed set,indicating that 9311 carries minor-effect Rfs for WA-type *** developed an advanced backcross population consisting of plants harboring small regions of donor chromosomal segments from 9311 in the WATianfeng A genetic background with moderate seed setting *** analysis showed that the pollen fertility levels of the backcross individuals are governed by a single gene from 9311 that we named Rf19(t).By use of the RICE 40 K gene chip,three introduced segments were identified in the fertile lines,and a candidate region spanning 4.37–8.29 Mb on chromosome 1 was identified for Rf19(t).Finally,Rf19(t)was fine-mapped to a region of 90 kb between the DNA marker loci STS1-163 and STS1-183,in which eight ORFs were ***,using relative expression analyses,comparative sequence analyses and functional domain analyses,we identified LOC_Os01g10530 as the most likely candidate gene for Rf19(t).Furthermore,Rf19(t)was found to function in fertility restoration,most probably by regulating the degradation of m RNA transcribed from the mitochondrial gene *** results increase our knowledge of fertility restoration in WA-type CMS lines and will facilitate the development of high-quality pairs of WAtype CMS and maintainer lines.

关键词： Rice WA-type CMS Restorer of fertility(Rf)gene Gene mapping

First detection of Diaporthe eucommiae causing leaf black blight disease on Cyclocarya paliurus

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First detection of Diaporthe eucommiae causing leaf black bl...

Genome-wide profiling of circular RNAs,alternative splicing,and R-loops in stem-differentiating xylem of Populus trichocarpa

中国植物病理学会第十一届全国会员代表大会暨2018年学术年会

作者： Jiang Dan He Huanhuan Peng xixu Tao Zong Xiao Ting Wang Haihua School of Life Science Hunan University of Science and Technology Key Laboratory of Ecological Remediation and Safe Utilization of Heavy Metal-polluted Soils College of Hunan Province

During the vegetation period(March to October) of 2013 and 2014,an outbreak of leaf black blight was observed on Cyclocarya paliurus plants in three plantations in Chengbu County,Hunan Province,China.A Diaporthe species was consistently isolated from the diseased leaflets or *** on the morphological characteristics of colony appearance,shape of conidia and conidiomata as well as sequences of internal transcribed spacer regions(ITS),the fungus was identified as Diaporthe *** test showed that *** isolates caused the symptoms originally observed on those of naturally infected plants,and the pathogenic isolates were successfully re-isolated from inoculated leaflets,thus fulfilling Koch’s *** is the first report of *** causing leaf black blight on *** screening indicated that the *** isolates were highly sensitive to thiophanate-methyl,chlorothalonil and iprodione,suggesting that practical use of these three fungicides has high potentials to control the leaf black blight on ***.

关键词： Diaporthe eucommiae Leaf black blight Identification of pathogen Cyclocarya paliurus

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Journal of Integrative Plant Biology 2021年第7期63卷 1294-1308页

作者： Xuqing Liu Yubang Gao Jiakai Liao Miao Miao Kai Chen Feihu Xi Wentao Wei Huihui Wang Yongsheng Wang xixu Anireddy S.N.Reddy Lianfeng Gu Basic Forestry and Proteomics Research Center College of ForestryFujian Provincial Key Laboratory of Haixia Applied Plant Systems BiologyFujian Agriculture and Forestry UniversityFuzhou 350002China Basic Forestry and Proteomics Research Center College of Life ScienceFujian Agriculture and Forestry UniversityFuzhou 350002China Department of Biology and Program in Cell and Molecular Biology Colorado State UniversityFort CollinsColorado 80523USA

Circular RNAs(circRNAs)are a recently dis-covered type of non‐coding RNA derived from pre‐mRNAs.R‐loops consist of a DNA:RNA hybrid andthe associated single‐stranded ***-dopsis thaliana,circRNA:DNA R‐loops regulatealternative splicing(AS)ofSEPALLATA3(SEP3).However,the occurrence and functions ofcircRNAs and R‐loops inPopulus trichocarpaarelargely ***,we performed circRNA‐enriched sequencing in the stem‐differentiatingxylem(SDX)*** identified 2,742distinct circRNAs,including circ‐CESA4,circ‐IRX7,and circ‐GUX1,which are generated from genesinvolved in cellulose,and hemicellulose biosyn-thesis,*** investigate the roles ofcircRNAs in modulating alternative splicing(AS),we detected 7,836 AS events using PacBio Iso‐Seq and identified 634 circRNAs that overlappedwith 699 AS ***,using DNA:RNAhybrid immunoprecipitation followed by se-quencing(DRIP‐seq),we identified 8,932 R‐looppeaks that overlapped with 181 circRNAs and 672AS ***,several SDX‐related circRNAsoverlapped with R‐loop peaks,pointing to theirpossible roles in modulating AS in ***,overexpressing circ‐IRX7increased the levels ofR‐loop structures and decreased the frequency ofintron retention in *** provides a valuable R‐loop atlas resourceand uncovers the interplay between circRNAs andAS in SDX ***.

关键词： alternative splicing circular RNA R‐loop Populustrichocarpa stem‐differentiating xylem