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Transcriptome profiling reveals the roles of pigment mechanisms in postharvest broccoli yellowing

Horticulture Research 2019年第1期6卷 846-859页

作者： Feng Luo Jia-Hui Cai xi-man kong Qian Zhou xin Zhou Ying-Bo Zhao Shu-Juan Ji Department of Food Science Shenyang Agricultural University110866 ShenyangPR Chin

Postharvest broccoli is prone to yellowing during storage,which is the key factor leading to a reduction in *** explore appropriate control methods,it is important to understand the mechanisms of *** analyzed the genes related to the metabolism of chlorophyll,carotenoids,and flavonoids and the transcription factors(TFs)involved in broccoli yellowing using transcriptome sequencing *** stored at 10℃showed slight yellowing on postharvest day 5 and serious symptoms on day *** were significant changes in chlorophyll fluorescence kinetics,mainly manifesting as a decrease in the Fv/Fm value and an increase in nonphotochemical quenching,during the yellowing *** sequencing profiles from samples of fresh broccoli and broccoli with slight and severe yellowing revealed 6,5,and 4 differentially expressed genes involved in chlorophyll metabolism,carotenoid biosynthesis,and flavonoid biosynthesis,*** transcription factor gene ontology categories showed that the MYB,bHLH,and bZip gene families were involved in chlorophyll *** addition,the transcription factor families included NACs and ethylene response factors(ERFs)that regulated carotenoid *** transcription polymerase chain reaction further confirmed that bHLH66,PIF4,LOB13,NAC92,and APL were vital transcription factors that potentially regulated the CAO and HYD genes and were involved in chlorophyll metabolism and the carotenoid biosynthetic *** flavonoid biosynthetic pathway was mainly regulated by MYBs,NACs,WRKYs,MADSs,and *** results of the differentially expressed gene(DEG)and pigment content analyses indicated that the transcriptome data were accurately and positively associated with broccoli yellowing.

关键词： Fv/Fm process fir

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Interaction of Plasma Proteins with Tri-quaternary Ammonium Salt Cationic Surfactant Studied by QCM-D

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Chinese Journal of Chemistry 2015年第2期33卷 253-260页

作者： man xi Baoyan Zhang Research Center for Molecular Science and Engineering Northeastern UniversityShenyangLiaoning 110819China

A tri-quaternary ammonium salt cationic surfactant was *** structure was confirmed by using Fourier-transform infrared spectroscopy,^(1)H nuclear magnetic resonance spectroscopy,and X-ray photoelectron spectroscopy *** model surfaces,including Au-CH_(3),Au-OH and Au-COOH,were *** of surfactant on the three model surfaces and subsequent plasma proteins adsorption were investigated by quartz crystal microbalance with dissipation(QCM-D).The mass of surfactant on the Au-COOH surface was the largest,followed by that on the Au-CH_(3)surface,and that on the Au-OH *** results suggested that the main driving force of surfactant immobilization was electrostatic interaction followed by hydrophobic *** on the results obtained,we concluded that the protein mass adsorbed on Au-CH_(3)-S,Au-OH-S,and Au-COOH-S surfaces depended on the protein size and *** mass and thickness of S on the Au-COOH surface is the largest and the protein adsorption capacity of Au-COOH-S surface is inferior to that of Au-CH_(3)-*** Au-COOH-S surface could inhibit lysozyme adsorption,maintain the adsorption balance of bovine serum albumin,and induce fibrinogen-binding protein adsorption.

关键词： tri-quaternary ammonium salt cationic surfactant self-assembled monolayers quartz crystal micro-balance with dissipation(QCM-D) proteins adsorption

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Relationship between high dietary fat intake and Parkinson’s disease risk: a meta-analysis

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Neural Regeneration Research 2019年第12期14卷 2156-2163页

作者： Yan Qu xi Chen man-man Xu Qiang Sun Department of Physiology School of Basic Medicine Qingdao University Qingdao Shandong Province China Intensive Care Unit The Affiliated Hospital of Qingdao University Qingdao Shandong Province China

OBJECTIVE: To assess whether dietary fat intake influences Parkinson’s disease risk. DATA SOURCES: We systematically surveyed the Embase and PubMed databases, reviewing manuscripts published prior to October 2018. The following terms were used:(“Paralysis agitans” OR “Parkinson disease” OR “Parkinson” OR “Parkinson’s” OR “Parkinson’s disease”) AND (“fat” OR “dietary fat” OR “dietary fat intake”). DATA SELECTION: Included studies were those with both dietary fat intake and Parkinson’s disease risk as exposure factors. The Newcastle-Ottawa Scale was adapted to investigate the quality of included studies. Stata V12.0 software was used for statistical analysis. OUTCOME MEASURES: The primary outcomes included the relationship between high total energy intake, high total fat intake, and Parkinson’s disease risk. The secondary outcomes included the relationship between different kinds of fatty acids and Parkinson’s disease risk. RESULTS: Nine articles met the inclusion criteria and were incorporated into this meta-analysis. Four studies scored 7 and the other five studies scored 9 on the Newcastle-Ottawa Scale, meaning that all studies were of high quality. Meta-analysis results showed that high total energy intake was associated with an increased risk of Parkinson’s disease (P = 0.000, odds ratio (OR)= 1.49, 95% confidence interval (CI): 1.26–1.75);in contrast, high total fat intake was not associated with Parkinson’s disease risk (P = 0.123, OR = 1.07, 95% CI: 0.91–1.25). Subgroup analysis revealed that polyunsaturated fatty acid intake (P = 0.010, OR = 1.03, 95% CI: 0.88–1.20) reduced the risk of Parkinson’s disease, while arachidonic acid (P = 0.026, OR = 1.15, 95% CI: 0.97–1.37) and cholesterol (P = 0.002, OR = 1.09, 95% CI: 0.92–1.29) both increased the risk of Parkinson’s disease. Subgroup analysis also demonstrated that, although the results were not significant, consumption of n-3 polyunsaturated fatty acids (P = 0.071, OR = 0.88, 95% CI: 0.73–1.05),α-linolenic ac

关键词： nerve regeneration dietary fat Parkinson's disease risk meta-analysis total energy intake polyunsaturated fatty acids arachidonic acid cholesterol α-linolenic acid linoleic acid n-3/n-6 polyunsaturated fatty acid intake ratio monounsaturated fatty acids neural regeneration

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CFD simulation of bubbly turbulent Tayor–Couette flow

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Chinese Journal of Chemical Engineering 2016年第6期24卷 719-727页

作者： xi Gao Bo kong R.Dennis Vigil Department of Chemical & Biological Engineering 2114 Sweeney Hall Iowa State University

Bubbly gas-liquid Taylor-Couette vortex flow has been the subject of several recent investigations both because of interest in bubble-induced drag reduction and because such devices have potential applications to a variety of chemical and biochemical processing problems. In order to quantitatively describe the hydrodynamics of highly turbulent two phase Taylor-Couette flow, a rigorous two-fluid computational fluid dynamics （CFD） model was developed and compared with previously published experimental data. This model includes a comprehensive description of the constitutive closure for inter-phase forces and turbulence was simulated using both the k- and k-to models. In addition, the mechanism by which the dispersed fluid attains a non-uniform radial and axial distribution is analyzed and the relative importance of various interphase forces is discussed. Lastly the model was validated by comparison of simulation predictions with experimental data, and it is shown that the CFD model correctly predicts phase velocity, velocity fluctuation, and gas distribution, and may provide guidance for reactor design and scale-up.

关键词： Taylor-Couette flowTwo-phase flowBubbleCFD

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An Improved Differential Fault Analysis on Block Cipher KLEIN-64

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Computers, Materials & Continua 2020年第11期65卷 1425-1436页

作者： Min Long man kong Sai Long xiang Zhang Changsha University of Science and Technology Changsha410014China School of Computing National University of SingaporeSingapore

KLEIN-64 is a lightweight block cipher designed for resource-constrained environment,and it has advantages in software performance and hardware *** investigation shows that KLEIN-64 is vulnerable to differential fault attack(DFA).In this paper,an improved DFA is performed to *** is found that the differential propagation path and the distribution of the S-box can be fully utilized to distinguish the correct and wrong keys when a half-byte fault is injected in the 10th *** analyzing the difference matrix before the last round of S-box,the location of fault injection can be limited to a small ***,this improved analysis can greatly improve the attack *** the best case,the scale of brute-force attack is only *** for the worst case,the scale of brute-force attack is far less than 232 with another half byte fault injection,and the probability for this case is 1/***,the measures for KLEIN-64 in resisting the improved DFA are proposed.

关键词： Block cipher KLEIN-64 differential fault analysis half-byte fault injection

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Inverse problems of the Erdos-Ko-Rado type theorems for families of vector spaces and permutations

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Science China Mathematics 2022年第5期65卷 1081-1108页

作者： xiangliang kong Yuanxiao xi Bingchen Qian Gennian Ge School of Mathematical Sciences Capital Normal UniversityBeijing 100048China School of Mathematical Sciences Zhejiang UniversityHangzhou 310027China

Ever since the famous Erd os-Ko-Rado theorem initiated the study of intersecting families of subsets,extremal problems regarding intersecting properties of families of various combinatorial objects have been extensively *** them,studies about families of subsets,vector spaces and permutations are of particular ***,we proposed a new quantitative intersection problem for families of subsets:For F([n]k),define its total intersection number as I(F)=ΣF1;F2∈F|F1∩F2|.Then,what is the structure of F when it has the maximal total intersection number among all the families in([n]k)with the same family size?In a recent paper,kong and Ge(2020)studied this problem and characterized extremal structures of families maximizing the total intersection number of given *** this paper,we consider the analogues of this problem for families of vector spaces and *** certain ranges of family sizes,we provide structural characterizations for both families of subspaces and families of permutations having maximal total intersection *** some extent,these results determine the unique structure of the optimal family for some certain values of jFj and characterize the relationship between having the maximal total intersection number and being ***,we also show several upper bounds on the total intersection numbers for both families of subspaces and families of permutations of given sizes.

关键词： total intersection number vector spaces permutations

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Small cell lung cancer with peripheral neuropathy as the first symptom:Two case reports

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World Journal of Clinical Cases 2025年第9期13卷 30-40页

作者： man Luo xiao-xi Lu Dan-Yang Meng Jin Hu Department of Neurology Affiliated Hospital of Jiaxing UniversityJiaxing 314000Zhejiang ProvinceChina Department of Information Technology Affiliated Hospital of Jiaxing UniversityJiaxing 314000Zhejiang ProvinceChina

BACKGROUND Small cell lung cancer(SCLC)is the most malignant type of lung *** in the latent period and early stage of the tumor,SCLC is prone to produce distant metastases with complex and diverse clinical *** is most closely related to paraneoplastic syndrome,and some cases present as paraneoplastic peripheral neuropathy(PPN).PPN in SCLC appears early,lacks specificity,and often occurs before diagnosis of the primary *** is easy to be misdiagnosed as a primary disease of the nervous system,leading to missed diagnosis and delayed diagnosis and *** SUMMARY This paper reports two cases of SCLC with limb weakness as the first *** first symptoms of one patient were rash,limb weakness,and abnormal *** patient was repeatedly referred to the hospital for limb weakness and rash for>1 year,during which time,treatment with hormones and immunosuppressants did not lead to significant improvement,and the condition gradually *** patient was later diagnosed with SCLC,and the dyskinesia did not worsen as the dermatomyositis improved after antineoplastic and hormone *** second case presented with limb numbness and weakness as the first symptom,but the patient did not pay attention to ***,the patient was diagnosed with SCLC after facial edema caused by tumor thrombus invading the ***,he was diagnosed with extensive SCLC and died 1 year after *** The two cases had PPN and abnormal electromyography,highlighting its correlation with early clinical indicators of SCLC.

关键词： Paraneoplastic peripheral neuropathy Small cell lung cancer Peripheral neuropathy Electromyography Dermatomyositis Case report

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Al-modified yolk-shell silica particle-supported NiMo catalysts for ultradeep hydrodesulfurization of dibenzothiophene and 4,6-dimethyldibenzothiophene:Efficient accessibility of active sites and suitable acidity

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Petroleum Science 2024年第1期21卷 654-666页

作者： Ke Yu Wei-Min kong Zhen Zhao Ai-Jun Duan Lian kong xi-Long Wang State Key Laboratory of Heavy Oil Processing China University of PetroleumBeijing102249China Institute of Catalysis for Energy and Environment College of Chemistry and Chemical EngineeringShenyang Normal UniversityShenyang 110034LiaoningChina

Yolk-shell SiO2 particles(YP)with center-radial meso-channels were fabricated through a simple and effective ***-containing YP-supported NiMo catalysts with different Al amounts(NiMo/AYP-x,x=Si/Al molar proportion)were prepared and dibenzothiophene(DBT)and 4,6-dimethyl-dibenzothiophene(4,6-DMDBT)were employed as the probes to evaluate the hydrodesulfurization(HDS)catalytic *** as-prepared AYP-x carriers and corresponding catalysts were characterized by some advanced characterizations to obtain deeper correlations between physicochemical properties and the HDS *** average pore sizes of series AYP-x supports are above 6.0 nm,which favors the mass transfer of organic *** cavity between the yolk and the shell is beneficial for the enrichment of S-containing compounds and the accessibility between reactants and active *** embedded into the silica framework could facilitate the formation of Lewis(L)and Brønsted(B)acid sites and adjust the metal-support interaction(MSI).Among all the as-synthesized catalysts,NiMo/AYP-20 catalyst shows the highest HDS *** improved HDS activity of NiMo/AYP-20 catalyst is attributed to the perfect combination of excellent structural properties of the yolk-shell mesoporous silica,enhanced acidity,moderate MSI,and good accessibility/dispersion of active components.

关键词： Al-containing yolk-shell silica particles Accessibility Acidity Metal-support interaction Hydrodesulfurization

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Advance in Studies of Dissolved Organic Carbon Flux in Wetland Ecosystem

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湿地科学 2013年第3期11卷 326-333页

作者： kong Fanting LI Yue xi Min kong Fanlong CHEN Wan College of Chemical and Environmental Engineering Qingdao University

Wetland ecosystem is an important part of the terrestrial ecosystem,and it plays a significant role in the global carbon *** wetlands are also major sources of dissolved organic carbon(DOC).The studies on DOC flux in wetlands have focused on its relation with carbon cycle *** was reported that there have been significant spatial and temporal variations of DOC in the wetlands associated with sloughs,streams and rivers in boreal and sub-boreal *** in DOC concentration and flux are considered to be related to a variety of factors affected by global climate change and human *** estimation of DOC concentration and flux from the terrestrial biosphere has been of great attention because of its importance for the status of carbon storage in the wetlands and the potential impact on global biogeochemical *** this paper,it is discussed the sources,compositions and characteristics of DOC,furthermore reviewed the research methods and the influential factors as well as some estimated models of DOC *** a conclusion,some suggestions and prospects for the direction of the future study have been addressed.

关键词：生态学环境地理学 DOC 二氧化碳

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Photodegradation of bisphenol A in Fe(Ⅲ)-oxalate complexes solution

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Journal of Environmental Sciences 2006年第4期18卷 771-776页

作者： ZHAN man-jun YANG xi xiAN Qi-ming kong Ling-ren State Key Laboratory of Pollution Control and Resource Reuse School of the Environment Nanjing University Nanjing 210093 China Jiaying University Meizhou 514015 China

The aqueous photodegradation of bisphenol A （BPA） in the presence of Fe（Ⅲ）-oxalate complexes （Fe（Ⅲ）-Ox）, which are common compositions of natural water, was investigated in this study. BPA underwent rapid indirect photolysis in Fe（Ⅲ）-Ox solution under simulated solar irradiation, proceeding pseudo-first-order kinetics. The photolysis rate increased with decreasing pH or initial BPA level and increasing Fe（Ⅲ）/oxalate concentration ratio. Hydroxyl radicals （·OH）, which were generated from the photochemical processes of Fe（Ⅲ）-Ox complexes and contributed to the photooxidation of BPA, were determined by molecular probe and electron spin resonance （ESR） methods with the steady-state concentration of 2.56 × 10^-14 mol/L. Superoxide anion radical （O2^·-） was considered as the precursor of. OH and qualitatively determined by adding nitro blue tetrazolium as well as ESR experiments. Based on the structural analysis of the intermediate photoproducts of BPA in Fe（Ⅲ）-Ox complexes solution, the possible degradation pathways of BPA were proposed, involving ·OH addition, alkyl scission and alky oxidation. The results indicate that the photochemical reactivity of Fe（Ⅲ） may affect the environmental fate of BPA in natural water significantly.

关键词： bisphenol A Fe(Ⅲ)-oxalate complexes photooxidation reactive oxygen species

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