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Nuclear microRNAs and their unconventional role in regulating non-coding RNAs

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Protein & Cell 2013年第5期4卷 325-330页

作者： Hongwei Liang Junfeng Zhang Ke zen.Chen-Yu Zhang xi Chen Jiangsu Engineering Research Center for microRNA Biology and Biotechnology State Key Laboratory of Pharmaceutical BiotechnologySchool of Life SciencesNanjing UniversityNanjing 210093China

MicroRNAs (miRNAs) are small non-coding RNAs (ncRNAs) that are involved in post-transcriptional gene regulation. It has long been assumed that miRNAs exert their roles only in the cytoplasm, where they recognize their target protein-coding messenger RNAs (mRNAs), and result in translational repression or target mRNA degradation. Recent studies, however, have revealed that mature miRNAs can also be transported from the cytoplasm to the nucleus and that these nuclear miRNAs can function in an unconventional manner to regulate the biogenesis and functions of ncRNAs (including miRNAs and long ncRNAs), adding a new layer of complexity to our understanding of gene regulation. In this review, we summarize recent literature on the working model of these unconventional miRNAs and speculate on their biological significance. We have every reason to believe that these novel models of miRNA function will become a major research topic in gene regulation in eukaryotes.

关键词： microRNA nuclear microRNA non-coding RNA nucleus Argonaute Exportin Importin

Immune modulatory function of abundant immune-related microRNAs in microvesicles from bovine colostrum

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Protein & Cell 2013年第3期4卷 197-210页

作者： Qi Sun xi Chen Jianxiong Yu Ke zen.Chen-Yu Zhang Liang Li Jiangsu Engineering Research Center for microRNA Biology and Biotechnology State Key Laboratory of Pharmaceutical BiotechnologyNanjing University School of Life SciencesNanjing 210093China Department of Virology University of California School of Public HealthBerkeleyCA 94720USA

Colostrum provides essential nutrients and immunologically active factors that are beneficial to *** previous work demonstrated that milk contains large amounts of miRNA that is largely stored in milk-derived microvesicles(MVs).In the present study,we found that the MVs from colostrum contain signifi cantly higher levels of several immune-related *** hypothesized that the colostrum MVs may transfer the immune-related miR-NAs into cells,which contribute to its immune modulatory *** isolated colostrum MVs by ultracentrifugation and demonstrated several immune modulation features associated with *** also provide evidence that the physical structure of milk-derived MVs is essential for transfer miRNAs and following immune modulation ***,we found that colostrum powder-derived MVs also contains higher levels of immune-related miRNAs that display similar immune modulation *** together,these results show that MV-containing immunerelated miRNAs may be a novel mechanism by which co-lostrum modulates body immune response.

关键词： colostrum miRNAs microvesicles immune modulation

Synthesis Characterization Non-isothermal Kinetics of the Thermal Decomposition and Redox Properties Derived from Copper(Ⅱ) Binuclear Coordination Compound of 1,4-Bis-(1'-Phenyl-3'-Methyl-5'-Pyrazolone-4')-1,4-Butanedione

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Chinese Chemical Letters 1997年第5期8卷 455-458页

作者： Cun SHAN Dian zen.JIA xi xiA(Department of Chemistry,, xinjiang University, Urumqi,830046). Department of Chemistry Xinjiang University Urumqi 830046 China

The paper reports the synthetic procedure and character of Copper(II) binuclearcoordination compound of 1,4-bis-(1'-phenyl-3'-methyl-5'-pyrazolone Thenon-isothermal kinetics of thermal decomposition of the complex has been stUdied from the TG-DTGcurves by means of the Achar et al. and Coats-Redfern methods,the most probab1e kinetic equation canbe expressed as dofdtrAe -E / RT * l /(2Q).The corresponding kinetic compensation effect expressions arefound to be lnuA=0. 1794E+0. *** non-isothermal thermal decomposition process of the complex isone-dimensional *** electrochemical studies of the complex(Cu2L'2)from cyclic voltamrnetriccurves by means of powder microelectrodes technique'',shows one two-electron irreversible process.

关键词： Methyl-5 Phenyl-3 Pyrazolone-4 Synthesis Characterization Non-isothermal Kinetics of the Thermal Decomposition and Redox Properties Derived from Copper Binuclear Coordination Compound of 1,4-Bis

Selective secretion of microRNA in CNS system

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Protein & Cell 2013年第4期4卷 243-247页

作者： Qipeng Zhang Jie Xu Qun Chen xi Chen Ke zen.Chen-Yu Zhang Jiangsu Engineering Research Center for microRNA Biology and Biotechnology State Key Laboratory of Pharmaceutical BiotechnologySchool of Life SciencesNanjing UniversityNanjing 210093China

EXTRACELLULAR miRNAs ARE PRESENT IN VARIOUS BODY FLUIDS MicroRNAs(miRNAs)are a diverse class of endogenous small non-coding RNAs,which posttranscriptionally regulate gene expression by interacting with their binding sites in target ***,several studies have demonstrated that miRNAs are also detectable outside cells,and these miRNAs may be called extracellular *** miRNAs are found in various body fluids,including plasma and serum(Chen et al.,2008),saliva(Park et al.,2009),amniotic fl uid(Weber et al.,2010),Bronchial lavage(Molina-Pinelo et al.,2012;Weber et al.,2010),sputum(xie et al.,2010),tears and urine(Weber et al.,2010),bile(Shigehara et al.,2011),seminal plasma(Wang et al.,2011),breast milk(Zhou et al.,2012),peritoneal fluid(Chen et al.,2012a).Finding the extracellular miRNAs extended the research field of miRNAs,and got a lot of fruitful research fi ndings.

关键词： al. Weber endogenous

Identification of Ebola virus microRNAs and their putative pathological function

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Science China(Life Sciences) 2014年第10期57卷 973-981页

作者： LIANG HongWei ZHOU Zhen ZHANG SuYang zen.Ke CHEN xi ZHANG ChenYu Jiangsu Engineering Research Center for microRNA Biology and Biotechnology State Key Laboratory of Pharmaceutical BiotechnologySchool of Life Sciences Nanjing University

Ebola virus(EBOV),a member of the filovirus family,is an enveloped negative-sense RNA virus that causes lethal infections in humans and ***,more than 1000 people have been killed by the Ebola virus disease in Africa,yet no specific treatment or diagnostic tests for EBOV are *** this study,we identified two putative viral microRNA precursors(pre-miRNAs)and three putative mature microRNAs(miRNAs)derived from the EBOV *** production of the EBOV miRNAs was further validated in HEK293T cells transfected with a pcDNA6.2-GW/EmGFP-EBOV-pre-miRNA plasmid,indicating that EBOV miRNAs can be produced through the cellular miRNA processing *** also predicted the potential target genes of these EBOV miRNAs and their possible biological ***,this study reports for the first time that EBOV may produce miRNAs,which could serve as non-invasive biomarkers for the diagnosis and prognosis of EBOV infection and as therapeutic targets for Ebola viral infection treatment.

关键词： microRNA Ebola virus function

Accurate quantification of 3'-terminal 2'-O-methylated small RNAs by utilizing oxidative deep sequencing and stem-loop RT-qPCR

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Frontiers of Medicine 2022年第2期16卷 240-250页

作者： Yan Kong Huanhuan Hu Yangyang Shan Zhen Zhou Ke zen.Yulu Sun Rong Yang Zheng Fu xi Chen Nanjing Drum Tower Hospital Center of Molecular Diagnostic and Therapy State Key Laboratory of Pharmaceutical BiotechnologyJiangsu Engineering Research Center for MicroRNA Biology and BiotechnologyNJU Advanced Institute of Life Sciences(NAILS)Institute of Artificial Intelligence BiomedicineSchool of Life SciencesNanjing UniversityNanjing 210023China Department of Urology Drum Tower HospitalMedical School of Nanjing UniversityInstitute of UrologyNanjing UniversityNanjing 210008China Department of General Surgery Drum Tower HospitalMedical school of Nanjing UniversityNanjing 210008China Research Unit of Extracellular RNA Chinese Academy of Medical SciencesNanjing 210023China

The continuing discoveries of novel classes of RNA modifications in various organisms have raised the need for improving sensitive,convenient,and reliable methods for quantifying RNA *** particular,a subset of small RNAs,including microRNAs(miRNAs)and Piwi-interacting RNAs(piRNAs),are modified at their 3'-terminal nucleotides via 2'-***,quantifying the levels of these small RNAs is difficult because 2'-0-methylation at the RNA 3'-terminus inhibits the activity of polyadenylate polymerase and T4 RNA *** two enzymes are indispensable for RNA labeling or ligation in conventional miRNA quantification *** this study,we profiled 3'-terminal 2'-0-methyl plant miRNAs in the livers of rice-fed mice by oxidative deep sequencing and detected increasing amounts of plant miRNAs with prolonged oxidation *** further compared the efficiency of stem-loop and poly(A)-tailed RT-qPCR in quantifying plant miRNAs in animal tissues and identified stem-loop RT-qPCR as the only suitable ***,stem-loop RT-qPCR was superior to poly(A)-tailed RT-qPCR in quantifying 3'-terminal 2'-0-methyl piRNAs in human seminal *** summary,this study established a standard procedure for quantifying the levels of 3'-terminal 2'-0-methyl miRNAs in plants and *** measurement of the 3'-terminal 2'-0-methylation of small RNAs has profound implications for understanding their pathophysiologic roles in biological systems.

关键词： small RNAs 2'-0-methylation sequencing RT-qPCR

Small RNA existed in commercial reverse transcriptase： primary evidence of functional small RNAs

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Protein & Cell 2015年第1期6卷 1-5页

作者： Jie Xu xi Chen Donghai Li Qun Chen Zhen Zhou Dongxia Hou Jin Wang Qipeng Zhang Ke zen.Chen-Yu Zhang Jiangsu Engineering Research Center for microRNA Biology and Biotechnology State Key Laboratory of Pharmaceutical Biotechnology School of Life Sciences Nanjing University Nanjing 210093 China

SMALL RNA IN REVERSE TRANSCRIPTASE： TOO SMALL TO BE FOUND Significant background in no-template or no-primer RT-PCR is frequently noted. However, the underlying mechanism is still unclear. In this issue, Jie Xu and colleagues reveal that the com- mercial reverse transcriptases contain small RNAs. In AMV reverse transcriptase, they detected microRNAs derived from the virus host chick or other birds. This surprising finding at least partially explains the high background in some AMV reverse transcriptases based RT-PCR.

关键词： Reverse Transcriptase Small RNAs miRNA Profile miRNA Expression Profile Solexa Sequencing

VlicroRNA-495 induces breast cancer ce migration by targeting JAM-A

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Protein & Cell 2014年第11期5卷 862-872页

作者： Minghui Cao Weiwei Nie Jing Li Yujing Zhang xin Yan xiaoxiang Guan xi Chen Ke zen.Chen-yu Zhang xiaohong Jiang Dongxia Hou State Key Laboratory of Pharmaceutical Biotechnology Jiangsu Engineering Research Center for microRNA Biology and Biotechnology School of Life Sciences Nanjing University Nanjing 210093 China Department of Medical Oncology Jinling Hospital School of Medicine Southern Medical University Guangzhou 510282 China The Comprehensive Cancer Center of Drum Tower Hospital Medical School of Nanjing University and Clinical Cancer Institute of Nanjing University Nanjing 210008 China

MicroRNAs （miRNAs） are small, non-coding RNAs that function as post-transcriptional regulators of gene expression. The deregulated expression of miRNAs is associated with a variety of diseases, including breast cancer. In the present study, we found that miR-495 was markedly up-regulated in clinical breast cancer samples by quantitative real time-PCR （qRT-PCR）. Junctional adhesion molecule A （JAM-A） was predicted to be a potential target of miR-495 by bioinformatics analysis and was subsequently verified by luciferase assay and Western blotting. JAM-A was found to be negatively correlated with the migration of breast cancer cells through loss-of-function and gain-offunction assays, and the inhibition of JAM-A by miR- 495 promoted the migration of MCF-7 and MDA-MB-231 cells. Furthermore, overexpression of JAM-A could restore miR-495-induced breast cancer cell migration. Taken together, our findings suggest that miR-4g5 could facilitate breast cancer progression through the repression of JAM-A, making this miRNA a potential therapeutic target.

关键词： miR-495 JAM-A breast cancer migration

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慧能禅学与阳明心学之比较

国学(汉斯) 2024年第4期12卷 633-638页

作者：席若凡贵州大学哲学学院贵州贵阳

六祖慧能为唐代禅宗高僧,虽为文盲却仍得禅宗最高法门,对当时及后世影响巨大,尤其是宋明理学,王阳明也在六祖慧能的禅学中汲取智慧,在王阳明的心学思想中都能看到六祖慧能禅学的思想。阳明心学在三教合一的背景下,汇各家之所长,被称之... 详细信息

六祖慧能为唐代禅宗高僧,虽为文盲却仍得禅宗最高法门,对当时及后世影响巨大,尤其是宋明理学,王阳明也在六祖慧能的禅学中汲取智慧,在王阳明的心学思想中都能看到六祖慧能禅学的思想。阳明心学在三教合一的背景下,汇各家之所长,被称之为儒中慧能,禅宗也被誉为佛教中的心学,这两者虽各有特点又有相同之处,本文尝试从两者的心性论与功夫论的思想内容比较其异同,使我们透过两位不同时代大能思想中体悟到禅学与儒学之间的内在关联,又能明了禅学与儒学之间不同的价值取向。

关键词：六祖慧能王阳明禅学儒学关联